2011 YÜksek lisans tez özetleri

New Generation Two Dımensıonal Lıquıd Chromotography (2d-Lc) System Usıng On Wheat (Triticum Aestivum L.) Proteomıcs Studıes

Proteom studies allow the analysis of all proteins which have a role in the biological process in a biological system at a particular phase. Proteomic is very important to  produce the efficient data about extremely complex response against to the biotic/abiotic stress in plants. However  in plants the number of the proteomic study is very  low compared with other organisms because of their rigid cell walls, complex and a wide variety of secondary metabolites, pigments, proteases, polyphenols, polysaccharides, starch and lipids. In the present thesis is aimed to reduce the metodological restirictions by using the new techonologies and contribute of the dissemination of the plant proteomics.

In a proteomic study, a protein mixture occurs as a result of disintegration of tissue which are composed of many different cells at different phases. First and most important step of proteomic is seperation of all proteins in this mixture. Two-dimensional gel electrophoresis (2D-PAGE) are widely used for his step. But this metod has lots of disadvantages such as loss of hidrofobic proteins, application difficulties, low reproducibility, take a long time vs. Therefore in recent year, two-dimensional liquid chromatography (2D-LC) systems are developed to provide faster, reliable and powerful separation of large number of total protein samples. In the scope of the thesis PF-2D which is a 2D-LC system, is optimized for using the plant proteomic studies. After completion of the optimization studies,  a model study carried out for demostrate the utility of the system

For this purpose, a study on wheat  was performed to determine of the proteins that have role on  resistance mechanism against to yellow rust disease which is caused by Puccinia striiformis f. sp. tritici. At the 72. hours after inoculation, the leaf samples were harvested from infected and non-infected control plants to isolate total proteins.  Afterwards the proteins are seprated by PF-2D system and compared one by one. In this comparasions differentialy expressed proteins were selected and identified by LC-ESI-MS/MS.  
  

NARİN Sevinç

Danışman : Prof. Dr. Şehnaz BOLKENT

Anabilim Dalı : Biyoloji

Programı : Zooloji

Mezuniyet Yılı : 2011

Tez Savunma Jürisi : Prof. Dr. Şehnaz BOLKENT

Prof. Dr. Seyhan ALTUN

Prof. Dr. Refiye YANARDAĞ

Doç. Dr. N. Ömür BULAN

Doç. Dr. Gül Özcan ARICAN