Aim: to set up a trans-channel centre for the elaboration of green intelligent “smart” biomaterials
tarix 29.10.2017 ölçüsü 445 b. #19982
Aim: to set up a trans-channel centre for the elaboration of green intelligent “smart” biomaterials Duration : October 2010 – 2013 University of Rouen Polymeres, Biopolymeres, Surfaces laboratory (PBS) , Dr L. Lebrun to prevent the growth of micro-organisms by using natural biocides or anti-biofilm molecules (polysaccharides and peptides produced by algae) to screen new molecules able to inhibit biofilm formation to share knowledge and complementary expertise of the partners to develop new support modification technologies In order to prevent colonization, many algae have developed chemical defences In order to prevent colonization, many algae have developed chemical defences variety of activities ( anti-bacterial, anti-inflammatory ….) Sustainable resources Biodiversity Availability of the biomass Known to be unfouled Green tide in Europe are becoming more frequent More than 100 000 m3 of Ulva sp . collected in North Brittany in 2011 Extract from Ulva sp : inhibitory effects on dinoflagellates (Jeong et al. 2000) and development of invertebrates (Johnson and welsh, 1985) Ulvans are structural acid polysaccharides present in cell wall of Ulva sp. , they represent 8-29% of the dry weight Ulva clathrata Invasive species : chemical defense strategy Undaria sp . and Sargassum sp . Fucoidan Polyphenol 96 wells-microplates 96 wells-microplates 4 concentrations : 0.01, 0.1,1 and 10 µg/ml 6 replicates Sterilization under UV 8 strains of bacteria : 100µl / well, incubation 48h at 24°C, measurement of OD620 nm and determination of Minimum Inhibition Concentration (MIC) (µg/ml) 3 strains of microphytobenthic microalgae Media F/2 (Guillard et al. 1962) Constant light 140 µmol m-2 s-1 T° 20° C Chlorophyll a extraction : Chlorophyll a extraction : Filtration of 10 ml of microalgae suspension Addition of methanol and extraction at 4°C in the dark Fluorescence measurement : excitation 485 nm – emission 645 nm Bioassay : 100 µL microalgal suspension (0.1 mg[Chl a ] L-1) 4 concentrations : 0.01, 0.1, 1, 10 µg/ml 6 replicates 5 days incubation : 20 °C, constant light 140 µmol m-2 s-1 Chlorophyll a extraction and measurement of fluorescence : excitation 485nm - emission 645 nm Determination of MIC ( µg/ml) All strains are inhibited by at least one product U3 show inhibition activity at low concentration against 3 of the vibrio strains Inhibition against v.aesturianus , a known pathogen for oyster : possible application in aquaculture 4 ulvans and 1 fucoidan inhibit V.harveyi , responsible for a disease that affects commercially farmed penaid prawns : possible application in aquaculture inhibition at low concentration against Pseudoalteromonas elyakovii U1-6 show growth inhibition activity against 4 of the strain tested Differents activities for ulvans extracted from U.armoricana 7 compounds show inhibition activies at low concentration against marine bacteria and microalgae 7 compounds show inhibition activies at low concentration against marine bacteria and microalgae Ulvans from U.compressa looks particularly promising Immobilisation of the most promising compounds on TiO2 Inclusion of the most promising compound in hydrogel Field assays University of Rouen Polymeres, Biopolymeres, Surfaces laboratory (PBS) , Dr L. Lebrun Dostları ilə paylaş:
