Basingstoke and north hampshire hospital pathology user handbook



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Reducing substances - faecal

 see Sugars







 

 

 

 




10 DAYS

Reducing substances - urine

 see Sugars







 

 

 

 




10 DAYS

Renin:

Lavender Top Vacuette

3mL

EDTA Plasma

 

mu/L

TO LAB AT ONCE - SAMPLE MUST BE FROZEN IN 30 MINUTES



Lab Note: SAS -20°C




Levels may be affected by posture, and antihypotensive or mineralosteroid drug therapy

Specialist Biochemistry

Mail Pont 8 Level C

South Block Southampton General Hospital

Tremona Road Southampton

SO16 6YD 02380 798717


14 DAYS

Supine

2 - 30

Ambulant

3 - 40

Children:

 

< 7d

4 - 340

1wk - 1yr

4 - 100

1 - 11yrs

4 - 85







Reticulin antibodies

see Coeliac screen







 

 

 

Lab Note: COEL 4°C




 







Ri antibodies

 see Purkinje







 

 

 

Lab Note: SAS -20°C

NEUR





 







Ro antibodies

 

 

see ENA

 

 




Lab Note: SAS 4°C

ANAE





 

 

 

RNP antibodies

 

 

see ENA

 

 

 

Lab Note: SAS 4°C

ANAE





 

 

 



TEST

SPECIMEN CONTAINER

VOLUME NEEDED

SAMPLE TYPE

REFERENCE RANGES

UNITS

SPECIAL PRECAUTIONS

KEY FACTORS

REFERRAL LABORATORY

(INC ADDRESS)

TURN – AROUND

(working days)

SAA

 see Amyloid A




 




 

 

 

 

 

 


Salicylate


Gold Top Vacuette

4mL

Serum

Analgesic 120 – 300

Toxic Risk >400


mg/L

 

 

 

4 HOURS


Scl 70 antibodies

 see AIP








 

 

 

Lab Note: SAS 4°C

ANAE





 

 

 

Selenium

Dark Blue

Vacuette


6mL

Serum

0.8 - 2.0

umol/L

 


Lab NB: Teklab -20ºC




 

Trace Metals Unit Chemical Pathology

Level D South Block Southampton General Hospital

Tremona Road Southampton

SO16 6YD

02380 796237


14 DAYS

Sex hormone binding globulin (SHBG)

Gold Top Vacuette

4mL

Serum

M 10 - 70

nmol/L

 

 




7 DAYS

F 30 - 120

Sirolimus

 Lavender Top

Vacuette


3mL

EDTA WB

 

 

 


Lab Note: WB 4ºC




 

Analytical Unit

St. Georges Hospital

London

SW17 0RE


0208 725 5345

14 DAYS

Skin antibodies

Gold Top Vacuette

4mL

Serum

 

 

 

Lab Note: SAS 4°C

PEMP





 

Southampton Immunology Mailpoint 8 Level C Southampton General Hospital

Tremona Road Southampton

SO16 6YD

02380 796229 or

02380 796650


10 DAYS

Slophylline

 see Theophylline

 




 

 

 

 








































TEST

SPECIMEN CONTAINER

VOLUME NEEDED

SAMPLE TYPE

REFERENCE RANGES

UNITS

SPECIAL PRECAUTIONS

KEY FACTORS

REFERRAL LABORATORY

(INC ADDRESS)

TURN – AROUND

(working days)




Sm antibodies

 see Liver antibodies







 

 

 

Lab Note: SAS 4°C

LIV





 

 

 




Smooth muscle antibodies

 see Liver antibodies







 

 

 

Lab Note: SAS 4°C

LIV








 

 

 




Sodium

Gold Top Vacuette

4mL

Serum

134 - 147

mmol/L

 

 

 

4 HOURS




Sodium - sweat

Plain 2mL Tube




Sweat

< 40

mmol/L

CONTACT LAB TO BOOK APPOINTMENT







24 HOURS




Sodium - urine 24h

Plain 24h bottle




Urine

130 - 220

mmol/d

24h Collection

Lab Note: White top Vacuette, Advia tray










24 HOURS




Sodium - urine

Plain (white top) universal

10mL

Urine




mmol/L

Random Sample

Lab Note: White top Vacuette, Advia tray










24 HOURS




Somatomedin C

 see IGF1







 

 
















Somatostatin

 see Gut Hormones







 

 
















Specific IgE

 see RAST







 

 










10 DAYS




SSA antibodies (Ro)

Gold Top Vacuette

4mL

Serum

_

_



Lab Note: SAS 4°C

ANAE








Southampton Immunology Mailpoint 8 Level C Southampton General Hospital

Tremona Road Southampton SO16 6YD 02380 796229 or

02380 796650


10 DAYS




SSB antibodies (La)

Gold Top Vacuette

4mL

Serum

_

_



Lab Note: SAS 4°C

ANAE








Southampton Immunology Mailpoint 8 Level C Southampton General Hospital

Tremona Road Southampton SO16 6YD 02380 796229

or 02380 796650


10 DAYS



TEST

SPECIMEN CONTAINER

VOLUME NEEDED

SAMPLE TYPE

REFERENCE RANGES

UNITS

SPECIAL PRECAUTIONS

KEY FACTORS

REFERRAL LABORATORY

(INC ADDRESS)

TURN – AROUND

(working days)

Steroid profile

Plain (white top) universal or 24hr plain bottle

10mL

Urine

 

 

Random Sample

or

24hr collection




Lab Note: Univ 4ºC







Dept. of Clinical Biochemistry

Kings College Hospital

Denmark Hill

London


SE5 9RS

0203 299 3856



28 DAYS

Sugars - faecal

Plain (orange top) specimen pot




Faeces

 

 

Deliver to lab immediately







10 DAYS

Sugars - urine

Plain (white top) universal

10mL

Urine

 

 

Deliver immediately








10 DAYS

Tacrolimus

Lavender Top Vacuette

3mL

EDTA Whole Blood

 Suggested therapeutic range

1.0 – 12.0



ug/L



Lab Note: WB 4ºC







Immunosuppressive Drug Monitoring Insitute of Liver Studies King's College Hospital Denmark Hill London SE5 9RS 0203 2993147

10 DAYS

Tau protein

 see Beta-2 transferrin







 

 











































Tegretol

 see

Carba-mazepine









 

 













Testosterone

Gold Top Vacuette

4mL

Serum

M 10.0 - 35.0



 

 











24 HOURS

F 0.5 - 2.6




TEST

SPECIMEN CONTAINER

VOLUME NEEDED

SAMPLE TYPE

REFERENCE RANGES

UNITS

SPECIAL PRECAUTIONS

KEY FACTORS

REFERRAL LABORATORY

(INC ADDRESS)

TURN – AROUND

(working days)

Thyroglobulin
& Anti -Thyroglobulin antibodies

Gold Top Vacuette

4mL

Serum

<1.0 post thyroid ablation

0-40 


ug/L

IU/L


 


Lab Note:SAS -20°C

TGAB





 

Chemical Pathology Lab

Level D


South Block Southampton General Hospital

Tremona Road Southampton

SO16 6YD 02380 796427


14 DAYS

TFT

Gold Top Vacuette

4mL

Serum

TSH

0.35 - 4.50



mu/L

 

TSH, FT3 and FT4 levels may all be affected by non-thyroidal illness

 

24 HOURS

Theophylline

Gold Top Vacuette

4mL

Serum

7.5 - 20.0

mg/L

Ideal sampling times: 1)oral: 8-12h post dose 2) IV: 6-8h after start of IV. Stop infusion for 15 minutes before taking sample

Adult levels only

 

24 HOURS

Thyroid peroxidase antibodies

Gold Top Vacuette

4mL

Serum

0 - 60

u/mL

 

 

 

24 HOURS

Tissue transglutaminase antibodies

Gold Top Vacuette

4mL

Serum

0 - 4

u/mL

 

Lab Note: COEL 4°C




 

 






TEST

SPECIMEN CONTAINER

VOLUME NEEDED

SAMPLE TYPE

REFERENCE RANGES

UNITS

SPECIAL PRECAUTIONS

KEY FACTORS

REFERRAL LABORATORY

(INC ADDRESS)

TURN – AROUND

(working days)

TMPG

Tubular Reabsorption

of Phosphate

Plain (white top) Universal

10mL

Urine

Birth 1.43–3.43

3-5m 1.48-3.30

6-23m 1.15-2.60

2-15y 1.15-2.44

Adult 0.80-1.35


mmol/L

glomer-ular

fluid


Must have paired blood sample for Phosphate and creatinine

Lab Note: Urine - white top vacuette , Advia tray










4 DAYS

Topiramate

Gold Top Vacuette

4mL

Serum

5 - 20

mg/L

 


Lab Note: SAS -20°C




 

Medical Toxicology Unit

4th Floor

North Wing

St Thomas’ Hospital

London SE1 7EH

02071 888689



10 DAYS

Total Protein

Gold Top Vacuette

4mL

Serum

63 - 79

g/L

 

Results may be increased by haemolysis

 

4 HOURS

Toxicology - General Screen

Plain (white top) universalplus

Lavender Top Vacuette

plus

Gold Top Vacuette



10mL

plus


1 x 3mL
plus

1 x 4mL


Urine

plus


EDTA

Whole Blood

plus

Serum


 

 

Random Sample


Lab Note: Univ 4ºC

Plus WB 4ºC



Plus SAS 4ºC




 

Medical Toxicology Unit

4th Floor

North Wing

St Thomas’ Hospital

London SE1 7EH

02071 888689



14 DAYS

TPMT

Thiopurine methyl transferase


Lavender Top Vacuette

3mL

EDTA Whole Blood

Normal 26 - 50. Carrier 10 - 25. Deficiency <10 

 

 

Lab Note: WB 4ºC




 

Purine Lab

4th Floor,

Block 7

North Wing

St Thomas’ Hospital

London SE1 7EH



02071 881265

21 DAYS



TEST

SPECIMEN CONTAINER

VOLUME NEEDED

SAMPLE TYPE

REFERENCE RANGES

UNITS

SPECIAL PRECAUTIONS

KEY FACTORS

REFERRAL LABORATORY

(INC ADDRESS)

TURN – AROUND

(working days)

Trace Metals


See Cu, Zn and Selenium


 




 

 

 

 

 

 

Transferrin

Gold Top Vacuette

4mL

Serum

1.8 - 3.2

g/L

 







4 HOURS

Transglutaminase antibodies

see Coeliac screen







 

 

 

Lab Note: COEL 4°C










10 DAYS

Triglycerides

Gold Top Vacuette

4mL

Serum

0.28 - 2.20

mmol/L

 

Patients must be fasting. There is some interference from haemolysis.




4 HOURS

1st or 2nd Trimester Screening

 See Triple Test







 

 

 










Triple Test

Red Top Vacuette

4mL

Serum

 

 

 


Lab Note: SAS -20°C

plus Stored -20°C

(unless small sample when all sent to QAH)








Chemical Pathology Queen Alexandra Hospital

Cosham Portsmouth

PO6 3LY 02392 286903


10 DAYS

Trimethylamine

Plain (white top) universal

10mL

Urine

 

 

Random Sample

(Early morning urine preferred)




Lab Note: Univ 4ºC







Clinical Chemistry and Molecular Genetics, Children's Hospital Western Bank Sheffield

S10 2TH 0114 2 71 7445



6 WEEKS


Troponin I


Gold Top Vacuette

4mL

Serum

< 0.05

ug/L

 







4 HOURS




TEST

SPECIMEN CONTAINER

VOLUME NEEDED

SAMPLE TYPE

REFERENCE RANGES

UNITS

SPECIAL PRECAUTIONS

KEY FACTORS

REFERRAL LABORATORY

(INC ADDRESS)

TURN – AROUND

(working days)

Tryptase

Gold Top Vacuette

4mL

Serum

2 - 14

ug/L

 

Lab Note: SAS -20ºC







Sheffield Protein Department of Immunology

Protein Reference Unit PO Box 894 Sheffield S5 7YT 0114 2 715552



14 DAYS

TSH

 see TFT








 

 

 







24 HOURS

TSH receptor

Antibodies

TRAB

Gold Top Vacuette

4mL

Serum

 

 

 


Lab Note: SAS -20°C

TRAB








Firs Laboratories

RSR Ltd


Parc Ty Glas

Llanishen

Cardiff

CF14 5DU

029 2076 5550


14 DAYS

UDPD

Plain (white top) universal

10mL

Urine

3.0 - 7.4

 

Random Sample


Lab Note: Univ -20ºC







Specialist Biochemistry

Mail Point 8 Level C

South Block Southampton General Hospital

Tremona Road Southampton

SO16 6YD

02380 798717



18 DAYS

Uniphylline

see Theophylline 







 

 

 










Urea

Gold Top Vacuette

4mL

Serum

2.6 - 6.0

mmol/L

 

Levels are greatly reduced during pregnancy




4 HOURS





TEST

SPECIMEN CONTAINER

VOLUME NEEDED

SAMPLE TYPE

REFERENCE RANGES

UNITS

SPECIAL PRECAUTIONS

KEY FACTORS

REFERRAL LABORATORY

(INC ADDRESS)

TURN – AROUND

(working days)

Urea - urine

Plain 24h bottle




Urine

199 - 332

 mmol/d

24h collection

Lab Note: White top Vacuette, Advia tray




 




24 HOURS

Uric acid - joint

Plain (white top) universal




Joint Fluid

 

mmol/L

 

 




24 HOURS

Uric acid - serum

Gold Top Vacuette

4mL

Serum

0.12 - 0.42

mmol/L

 

 




4 HOURS

Uric acid - urine

Plain 24h bottle




Urine

1.48 - 4.43

mmol/d

24h collection

Lab Note: White top Vacuette, Advia tray




 




24 HOURS

Urobilinogen

 




see Bile Pigments

 

 

 

 




4 HOURS

Urine Steroid Profile (USP)

See steroid profile

























U&E - serum

Gold Top Vacuette

4mL

Serum

Na 134 - 147

K 3.5 - 5.0

Urea 2.6 - 6.0

Creat 62 -124 



 mmol/L

 

 




4 HOURS

1 Hour for urgent

U&E - urine

Plain 24h bottle




Urine

Na 130 – 220

K 25 – 100



Urea 199 -332 

mmol/d 

24h collection

Lab Note: White top Vacuette, Advia tray




 




24 HOURS

Valproate

Gold Top Vacuette

4mL

Serum

50 - 100

mg/L

Take sample before next dose.



Standardise sampling times if possible as levels may vary during the day




24 HOURS

Vancomycin

Gold Top Vacuette

4mL

Serum




mg/L










24 HOURS


Very long chain fatty acids

Gold Top Vacuette

4mL

Serum

 

 

 


Lab Note: SAS -20°C




 

Newborn Screening & Biochem Genetics Southmead Hospital Westbury-on-Trym Bristol BS10 5NB 0117 3235556

21 DAYS

Pristinic acid

Phytanic acid



TEST

SPECIMEN CONTAINER

VOLUME NEEDED

SAMPLE TYPE

REFERENCE RANGES

UNITS

SPECIAL PRECAUTIONS

KEY FACTORS

REFERRAL LABORATORY

(INC ADDRESS)

TURN – AROUND

(working days)

Vigabatrin

Gold Top Vacuette

4mL

Serum

5-35 

 mg/L

 


Lab Note: SAS -20°C




 

Medical Toxicology Unit

4th Floor

North Wing

St Thomas’ Hospital

London SE1 7EH

02071 888689



28 DAYS

VIP

 see Gut Hormones







 

 

 

 

 

 

Vitamin A

Green Top Vacuette

4mL

Li Heparin

Plasma


1.07 – 3.55

umol/L

 


Lab Note: SAS -20°C




 

Chemical Pathology Lab

Level D


South Block Southampton General Hospital

Tremona Road Southampton

SO16 6YD 02380 796427


14 DAYS

Vitamin B12

Gold Top Vacuette




4mL

Serum

161 - 531

ng/L

 

 




3 DAYS

Vitamin D

Gold Top Vacuette

4mL

Serum

See report for interpretive comments

nmoL/L


Lab Note: SAS -20°C




 

Homerton University Hospital

Homerton Row

London

E9 6SR


0208 510 7887

10 DAYS



TEST

SPECIMEN CONTAINER

VOLUME NEEDED

SAMPLE TYPE

REFERENCE RANGES

UNITS

SPECIAL PRECAUTIONS

KEY FACTORS

REFERRAL LABORATORY

(INC ADDRESS)

TURN – AROUND

(working days)

Vitamin D –

1,25 di(OH)

DHVD

Gold Top Vacuette

4mL

Serum

72 - 125 

pmol/L

MUST BE RECEIVED IN LAB WITHIN 30 MINUTES


Lab Note: SAS -20°C




 

Specialist Biochemistry

Mail Point 8 Level C

South Block Southampton General Hospital

Tremona Road Southampton

SO16 6YD 02380 798717


10 DAYS

Vitamin E

Green Top Vacuette

4mL

Plasma

11.6 – 37.1

umol/L




Lab Note: SAS -20°C




 

Chemical Pathology Lab

Level D


South Block Southampton General Hospital

Tremona Road Southampton

SO16 6YD 02380 796427


14 DAYS

VLCFA

see Very long chain fatty acids







 .

 

 

 

 

21 DAYS

VMA/Metadrenalins (adult)

Acidified 24h Urine bottle




Urine

 

 

umol/d


24h collection

Lab Note: Univ 4ºC




Levels may be affected by antihypertensive and antiprophylatic drug therapy and stress

Dept of Chemical Pathology

Level D


South Block Southampton General Hospital

Tremona Road Southampton

SO16 6YD 02380 796427


14 DAYS

Adrenaline

0 – 0.1

Dopamine

0.49 – 2.85

Metadrenaline

< 1.4

Noradrenaline

0.07 – 0.48

Normetadrenaline

< 3.0



TEST

SPECIMEN CONTAINER

VOLUME NEEDED

SAMPLE TYPE

REFERENCE RANGES

UNITS

SPECIAL PRECAUTIONS

KEY FACTORS

REFERRAL LABORATORY

(INC ADDRESS)

TURN – AROUND

(working days)

VMA (child)

+ HVA

Plain (white top) universal

10mL

Urine

Age related  

umol/mmolCr

Random sample MUST BE RECEIVED IN LAB WITHIN

1 HOUR


Lab Note: Univ 4ºC




 

Chemical Pathology

Level D


South Block Southampton General Hospital

Tremona Road Southampton

SO16 6YD 02380 796427


14 DAYS

Voltage-gated Ca antibodies

Gold Top Vacuette

4mL

Serum

 

 

 



Lab Note: SAS -20°C

VGCC





 

Immunology

Churchill Hospital

Headington

Oxford


OX3 7LJ

01865 225995



28 DAYS

Voltage-gated K antibodies

Gold Top Vacuette

4mL

Serum

 

 

 


Lab Note: SAS -20°C

VKAB





 

Immunology

Churchill Hospital

Headington

Oxford


OX3 7LJ

01865 225995



18 DAYS

White cell enzymes

see Lysosomal enzymes







 

 

 

 








Yo antibodies

 see Purkinje







 

 

 

Lab Note: SAS -20°C

NEUR





 







Xanthochromia

Universal

1 mL

CSF






See Clinical procedure



Lab Note: Special 4ºC













Zinc

Dark Blue

Vacuette


6mL

Plasma

11.0 – 24.0

umol/L

 

Must be bled in Phlebotomy



Lab NB: Teklab -20ºC

Li Hep may be used for Paeds






 

Trace Metals Unit Chemical Pathology

Level D


South Block Southampton General Hospital

Tremona Road Southampton

SO16 6YD 02380 796237


14 DAYS



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