HAEMATOLOGY |
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TEST
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SAMPLE & COLLECTION INSTRUCTIONS
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REFERENCE RANGE
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UNITS
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COMMON INDICATIONS
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Turnaround time (working days)
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Antenatal screen
(Group and antibody screen)
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Pink top 6ml Transfusion tube
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1
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Antibodies (specific identification)
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Pink top 6ml Transfusion tube
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2
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Blood Group and save serum
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Pink top 6ml Transfusion tube
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1
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Bone marrow film
(with Trephine biopsy)
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Please contact Clinical Haematologist for patient review for potential bone marrow
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Haematological malignant disorders
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2
7
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Cold Agglutinins
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Pink top 6ml Transfusion tube (keep sample at 37oC)
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< 1/32
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Cold agglutinin disease; Raynaud’s
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2
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Direct Antiglobulin Test
(Coombes Test)
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Lavender 3ml EDTA tube
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Haemolysis - antibody mediated
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4 hours
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Donath Landsteiner (PCH)
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Pink top 6ml Transfusion tube - Contact Consultant
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2
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Genotypes and Phenotypes
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Pink top 6ml Transfusion tube
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1
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HLA B27
(HLA B27 samples received Monday to Thursday only; to reach BNHFT Haematology Dept. by 12 noon the same day the sample is collected, as it is sent to the Regional Centre in Southampton).
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1 x large 6ml Lavender top EDTA tube or 2 x 3ml Lavender topped EDTA tubes
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Ankylosing spondylitis
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21
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Kleihauer test
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Pink top 6ml Transfusion tube
and lavender top EDTA tube
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Trans-placental bleed
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4 hours
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Anti Xa assay
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Light Blue citrate tube
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iu/mL
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Sub cutaeous Heparin monitoring
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On request
or 4
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Beta 2 Microglobulin
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Yellow SST gel tube
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0.0 – 1.7
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ug/mL
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10
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Bleeding time
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Appointment required - please ring Principal BMS Jane Needham
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3.5 – 9.5
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min
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TEST
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SAMPLE & COLLECTION INSTRUCTIONS
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REFERENCE RANGE
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UNITS
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COMMON INDICATIONS
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Turnaround time (working days)
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Chromosomes
Refer - please speak to laboratory
(samples received Monday to Thursday only; to reach the Department by 4.00 pm the same day the sample is collected, as it is sent to the Regional Centre in Salisbury)
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14
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CSF
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Cerebal Spinal fluid
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Coagulation screen
Prothrombin Time
INR Therapeutic
APTT
APTT Therapeutic
Thrombin time
Fibrinogen
Fibrinogen Antigen
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Light Blue top citrate tube
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10.9 – 13.9
2 - 4
25.4 – 34.6
75.0 – 125.0
11.2 – 13.6
1.5 – 4.0
1.43 – 3.55
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secs
secs
secs
secs
g/L
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Bleeding; bruising
Pre operation
Warfarin control
Heparin monitoring
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3 hours
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D-Dimer
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Light Blue citrate tube
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<0.0 – 0.5
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mg/L
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Fibrinolysis; DIC; DVT
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3 hours
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ESR
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Lavender top 3ml EDTA tube
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Male < 20
Female < 20
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mm/hr
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Temporal arteritis; Inflammatory marker
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4 hours
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Factor assays
Factors 2, 5, 7, 9, 10,11,12
Factor 13
Factor 13 antigen
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2 x Light Blue top citrate tubes
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50 – 150
>2
10.0 – 17.3
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iu/dL
iu/dL
mg/L
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Investigation of abnormal coagulation
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14
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Factor Inhibitor
Factors VIII, IX, porcine inhibitors, screens
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2 x Light Blue top citrate tubes
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Bethesda
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Investigation of abnormal coagulation
Not response to factor therapy
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On request or
14
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FBC (Full Blood Count)
[includes blood film
if required]
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Lavender top 3ml EDTA tube
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Male
Hb 130 - 180
Female
Hb 110 - 165
WBC 4.0 - 11.0
Platelets 150 - 500
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g/L
g/L
10*9/L
10*9/L
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4 hours
[With blood film 3 -5 days]
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TEST
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SAMPLE & COLLECTION INSTRUCTIONS
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REFERENCE RANGE
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UNITS
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COMMON INDICATIONS
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Turnaround time
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G6PD
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Lavender top 3ml EDTA tube -
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4.6 – 13.5
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u/g Hb
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Jaundice; Haemolysis post infection
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2
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Glandular Fever screen
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Lavender top 3ml EDTA tube
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Infectious mononucleosis; EBV
May be positive in lymphoma & others
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4 hours
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Hb HPLC
Hb electrophoresis
HbA2
HbF
HbS, HbC, HbD, HbE
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Lavender top 3ml EDTA tube
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1.6 – 3.1
0.1 – 1.0
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%
%
%
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Haemoglobinopathy
Thalassaemia
Antenatel and newborn screening programme
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3
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Abnormal Haemoglobins
Sample for reference Centre
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2x Lavender top 3ml EDTA tube
Sent to Haemoglobin reference centre Oxford
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Dependant on abnormality.Up to 3 months
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Haptoglobin assay
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Yellow top SST gel tube
To arrive at BHNFT Haematology by 12 noon on day sample is taken as sample sent to Southampton
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0.7 - 3.8
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g/L
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Haemolysis marker
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4
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Heparin Induced Thrombocytopenia HITS
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Yellow top SST gel tube
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Platelet count dropping by 50% in 24 hours when heparin infusion started
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On request or 7
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INR (International
normalised ratio)
and warfarin dosing
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Light Blue citrate Tube
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Therapeutic
2.0 - 4.0
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Warfarin control
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4 hours
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NAP test
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Lavender top EDTA tube
- Discuss with laboratory
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30 - 100
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2
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Lupus inhibitor
Silca clot time
Dilute Russel Viper Venom time
IgM Cardiolipin
IgG Cardiolipin
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4 x Light Blue citrate tubes
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0.8 – 1.2
0.8 – 1.2
0 – 12
0 – 13
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MPL
GPL
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14
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Red cell Parasities
Malaria film
Malaria antigens
Microfilariae
Trypanosomes
Leishmaniasis
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Lavender top EDTA tube to Haematology for Film.
Positive results sent to Hospital for Tropical Diseases
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4 hours
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TEST
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SAMPLE & COLLECTION INSTRUCTIONS
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REFERENCE RANGE
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UNITS
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COMMON INDICATIONS
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Turnaround time
(Working days)
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PK (Pyruvate kinase - red cell enzyme)
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Lavender top EDTA tube - Contact haematology
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Pyruvate kinase deficiency
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4
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Paroxysmal Noctural Haemoglobinuria
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Lavender top EDTA tube.
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100
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%
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Haemolysis – anaemia
Haemoglobinuria
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2
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Platelet Function Analysis
Collagen / EPI
Collagen / ADP
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2 x Light Blue citrate tubes
Test need to be done ASAP after collection. Please liaiase with Coagulation ext 3294
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87 – 167
59 – 111
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Secs
Secs
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3 hours
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Platelet Aggregation
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Special tubes and by appointment only; please contact the Principal BMS Jane Needham
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Red cell survival studies
Red cell membrane studies
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Special tubes and by appointment only; please contact the Consultant Haematologist
Sent to Bristol
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Up to two months
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Reticulocyte count
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Lavender top EDTA tube
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0.5 - 2.5%
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109/l
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Red cell production; Aplasia;
Bleeding haemolysis
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4 hours
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Rheumatoid factor
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Yellow top gel tube
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RA
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2
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Sickle test
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Lavender top EDTA tube
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3 hours
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Thromboelastography
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2 x Light Blue citrate tubes
Test need to be done ASAP after collection. Please liaiase with Coagulation ext 3294
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4 hours
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Thrombophilia screen:
Antithrombin III
Protein C
Protein S Free
APCR
FVIIIc
FV Leiden
ProtrombinGene Mutation
MTHFR
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4 x Light blue citrate and 2 x Lavender EDTA tubes
Sent to Hammersmith
Sent to Hammersmith
Sent to Cambridge
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81.4 – 126.6
88.3 – 163.5
3.5 – 10.4
2.5 – 4.9
50 – 150
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iu/dl
iu/dl
iu/dl
l/l
%
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Thrombophilia
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On request or 14
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TEST
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SAMPLE & COLLECTION INSTRUCTIONS
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REFERENCE RANGE
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UNITS
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COMMON INDICATIONS
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Turnaround time
Working days)
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Additional Thrombophilia tests
Anti Thrombin III antigen
Protein C Antigen
Total Protein S
Plasminogen
Homocysteine
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4 x Light blue citrate
Other rare Thrombophilia tests are available on request please discuss with Principal BMS Jane Needham
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224.0 – 367.0
2.40 – 4.64
19.1 – 34.3
93.8 – 147.8
Male 5.64 – 14.88
Female3.0 –12.52
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mg/L
mg/L
ug/mL
iu/dl
umol/L
umol/L
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On request or 14
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von Willebrand’s screen VWF
VIIIc
VWFAntigen
VWF Ristocetin co-factor
VWF Normandy
VWF Collagen binding
VIII Multimers
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3 x Light Blue citrate tubes
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50 – 150
50 – 150.0
50 – 150
50 – 150
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iu/dL
iu/dL
iu/dL
iu/dL
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von Willebrand’s disease
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14
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White cell markers
CD3
CD4
CD8
CD16+56
CD19
LEUK
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1 Lavender EDTA
samples received Monday to Thursday
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0.72 – 2.74
0.40 – 1.61
0.22 – 1.12
0.08 – 0.72
0.08 – 0.61
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10*9/L
10*9/L
10*9/L
10*9/L
10*9/L
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On request or 2
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