Basingstoke and north hampshire hospital pathology user handbook

SEROLOGY

TEST	SAMPLE & COLLECTION INFORMATION	REFERENCE RANGE	UNITS	COMMON INDICATORS	TURNAROUND TIMES (WORKING DAYS)
Alpha virus	Gold top gel tube				Referred - 21 Days
Adenovirus antibody	Refer to viral antibody screen
Amoebic IFAT	Gold top gel tube and a faeces sample required also				Referred - 21 Days
Antenatal screen (Rubella IgG, HIV, Hepatitis B surface antigen, Syphilis)	Gold top gel tube (request form MUST be signed)				8 (max) Tests performed on WEDNESDAY only.
Antistaphylolysin	Gold top gel tube			Staphylococcal disease	Referred - 21 Days
Antistreptolysin O (ASO)	Gold top gel tube			Streptococcal disease	Referred - 21 Days
Aspergillus precipitins	Gold top gel tube			Farmers lung	Referred - 21 Days
Avian precipitins	Gold top gel tube				Referred - 21 Days
Bartonella antibodies	Gold top gel tube			Cat scratch disease	Referred - 21 Days
Bilharzia antibodies	Gold top gel tube
Bordetella pertussis antibodies	Gold top gel tube				Referred - 21 Days
Borrelia burgdorferi antibodies	Gold top gel tube Collection 8 weeks after bite.			Lyme disease	1
Brucella antibodies	Brown top 4.7ml gel tube			Brucellosis	Referred - 21 Days
Cat scratch disease	Refer to Bartonella antibodies				21 Days

TEST	SAMPLE & COLLECTION INFORMATION	REFERENCE RANGE		UNITS	COMMON INDICATORS	TURNAROUND TIMES (WORKING DAYS)
Chlamydia psittaci	Refer to viral antibody screen
Chlamydia Trachomatis antibodies	Gold top gel tube (By arrangement ONLY)					Referred - 21 Days
Cytomegalovirus antigen	Urine in a 25ml sterile universal container or respiratory secretions					Referred - 21 Days
Cytomegalovirus IgM	Gold top gel tube					1
Cytomegalovirus IgG	Gold top gel tube					1
Dengue antibody	Gold top gel tube					Referred - 21 Days
Enterovirus IgM (includes antibodies to Coxsackie A and B and Echovirus infections)	Gold top gel tube					Referred - 21 Days
Epstein Barr virus antibodies	Gold top gel tube					Referred - 21 Days
Filarial antibodies	Gold top gel tube					Referred - 21 Days
Galactomanan	Gold top gel tube					Referred - 21 Days
Haemophilus B antibody	Gold top gel tube					Referred - 21 Days
Helicobacter pylori antigen	Stool sample in 60ml universal					8 Testing performed Friday ONLY

TEST	SAMPLE & COLLECTION INFORMATION	REFERENCE RANGE	UNITS	COMMON INDICATORS	TURNAROUND TIMES (WORKING DAYS)
Hepatitis A IgG and IgM	Gold top gel tube				1
Hepatitis B surface antigen (NOT Antenatal)	Gold top gel tube				7
Hepatitis B antibodies	Gold top gel tube. 10 weeks post vaccination			Immunity	8 Performed Tuesday ONLY
Hepatitis B markers	Gold top gel tube				1 after +ve screen
Hepatitis B viral load	Gold top gel tube				14
Hepatitis C antibody	Gold top gel tube				5 Mon – Thurs ONLY
Hepatitis C viral load	Gold top gel tube				Referred -21 Days
HIV 1 and 2 antibodies (NOT Antenatal)	Gold top gel tube (request form MUST be signed and state Patient has consented to test)				1
HIV viral load	Lavender top EDTA tube				Referred -21 Days
Influenza A antibodies	Refer to viral antibody screen
Influenza B antibodies	Refer to viral antibody screen
Legionella antibodies	Gold top gel tube			Atypical pneumonia	Referred -21 Days
Legionella urinary antigen	25ml sterile universal				1
Leishmania antibodies	Gold top gel tube				Referred -21 Days
Leptospira antibodies	Gold top gel tube				Referred -21 Days
Malaria	Lavender top EDTA to haematology for Film and call Microbiologist for advice. Antibodies rarely required.

TEST	SAMPLE & COLLECTION INFORMATION	REFERENCE RANGE	UNITS	COMMON INDICATORS	TURNAROUND TIMES (WORKING DAYS)
Measles antibodies IgG IgM	Gold top gel tube Gold top gel tube			Ring CCDC if suspected	1 Referred -21 Days
Meningococcal PCR	Lavender top EDTA tube. By prior arrangement with the laboratory.			Meningococcal disease Ring CCDC.	Referred -21 Days
Mumps antibodies	Gold top gel tube			Ring CCDC if suspected.	Referred -21 Days
Mycoplasma antibodies	Refer to viral antibody screen				Referred -21 Days
Parvovirus B19 IgM	Gold top gel tube			Suspected infection in pregnancy	Referred -21 Days
Q fever (Coxiella burnetii) antibodies	Refer to viral antibody screen			Puo flu like illness, returning traveller, animal exposure, culture negative Endocarditis	Referred -21 Days
RSV antibodies	Refer to viral antibody screen
Rubella IgG	Gold top gel tube			Immunity	7
Rubella IgM	Gold top gel tube. Date of onset essential.			Infection. If suspected ring CCDC	Referred -21 Days
Schistosome antibodies	Gold top gel tube			Bilharzia	Referred -21 Days
Strongyloides serology	Gold top gel tube				Referred -21 Days
Syphilis serology	Gold top gel tube			Infection	10

TEST	SAMPLE & COLLECTION INFORMATION	REFERENCE RANGE	UNITS	COMMON INDICATORS	TURNAROUND TIMES (WORKING DAYS)
ToRCH screen (Toxoplasma, Rubella IgM and IgG, CMV IgM, Herpes)	Gold top gel tube				Referred -21 Days
Toxoplasma antibodies	Gold top gel tube			Infection	2
Tropical screen	Gold top gel tube – Discuss with Microbiologist
Varicella zoster antibodies	Gold top gel tube			Immunity	1
Viral respiratory antibody screen	Gold top gel tube, collected between day 1-3 (acute) and day 10-21 (convalescent). The acute specimen will be stored until the convalescent is received. (Adenovirus, Chlamydia psittaci, CMV, C.burnetii, Influenza A and B, RSV, Mycoplasma). The range of investigations performed will depend on clinical details; accurate and full information will ensure the relevant tests are performed.				Referred -21 Days
Yersinia antibodies	Gold top gel tube				Referred -21 Days