Component 8 Research & Innovation Colleges

Partners

S. No	Name of the Industry	Roles and responsibility
1	ITC Life Sciences and Technology Centre, Bengaluru	Formulation of identified active biomolecules and Technology Transfer Formulation of the active biomolecules in cosmetic products
2	Tablets India Limited, Chennai	Field trials of novel bioactive molecules against aquaculture pathogens

2. 
   Definition and significance/value of concreteoutcomes
   

In order to sustain the development and to foster Science & Technology further, we need to improve further in terms of Research Infrastructure, procurement of vital equipment. Thus, the infrastructure/equipment/ resources envisaged through RUSA will further augment the Research potential of the Researchers and will enhance the quality of research output to International standards.

2. 
   Inter-disciplinary/multi-disciplinary orientation and multi-institutionnetworking
   

The work envisaged under RUSA aims to strengthen the existing research capability of the Coordinator’s Group through interdisciplinary research by joining hands with researchers from the various University. Hence, the proposed objectives and targets can be achieved with the available complementary expertise within the stipulatedtime

2. 
   Anticipated Outcome (s) of the proposal
   

1. 
   Identification of new bioactive molecules from marine and other natural sources that may act as potential alternates for antibiotics in the treatment of several microbial related pathogenesis affecting human and animals and validation through in vitro and in vivo systems
   
2. 
   Bioactive lead molecules with immune enhancing property will be madeavailable
   
3. 
   Mass propagation of hairy roots from Indian medicinal plants for value rich therapeutically important secondary metabolites.
   
4. 
   Development of iPSC technology for in vitro modelling for neurologicaldiseases.
   

5. 
   Development of economically viable organic and metallo-drugcandidates
   

6. 
   Production of coatings with improved corrosion resistance, toughness, self lubricating properties and better wear & tear resistance
   
7. 
   Process or Product patent for novel bioactive molecules.
   

2. 
   Research and Innovation culture creation among affiliatedcolleges
   

• 
  Workshops and Hands on training will be provided to the faculty members working in the area of biosciences of affiliated colleges
  
• 
  Faculty members of the affiliated colleges will be encouraged to utilize the facilities created under this programme
  
• 
  The expertise developed under this scheme will be transferred to the benefit of Faculty members of the affiliated colleges to augment the research projects from the funding agencies and Industrial partners
  
• 
  Research Guidance will be provided to the Students/Faculties of the affiliated colleges/neighboring Institutions
  

Publications relevant to the Focus Area (Health- New bioactive molecules for humanand

animal health)

1. 
   Gowrishankar S, Kamaladevi A, Ayyanar KS, Balamurugan K and Pandian SK (2015). Bacillus amyloliquefaciens-secreted cyclic dipeptide- cyclo (L-Leucyl- L- Prolyl) inhibits biofilm and virulence in methicillin-resistant Staphylococcus aureus. RSC Advances [Royal Society of Chemistry] (Impact Factor.3.84) (inpress).*
   
2. 
   Sethupathy S, Nithya C and Pandian SK (2015). 2-furaldehyde diethyl acetal from tender coconut water (Cocos nucifera L: Arecaceae) attenuates biofilm formation and quorum sensing-mediated virulence of Chromobacterium violaceum and Pseudomonas aeruginosa. Biofouling [Taylor & Francis, UK] (Impact Factor: 3.415) (in press).*
   
3. 
   Sethupathy S, Shanmuganathan B, Devi KP and Pandian SK (2015). Alpha-bisabolol from brown macroalga Padina gymnospora mitigates biofilm formation and quorum sensing controlled virulence factor production in Serratia marcescens. Journal of Applied Phycology [Springer] (IF- 2.559) (in press).*
   

4. Padmavathi AR, Bakkiyaraj D, Thajuddin N and Pandian SK (2015). Effect of 2, 4-di- tert-butylphenol on the growth and biofilm formation of an opportunistic fungus Candida albicans. Biofouling.[Taylor & Francis, UK] (Impact Factor: 3.415) (in press).*

5. 
   Sivasankar C, Ponmalar A, Bhaskar JP and Pandian SK (2015). Glutathione as a promising Anti-Hydrophobicity agent against Malassezia spp. Mycoses [Wiley] (Impact Factor: 2.239) (in press).*
   
6. 
   Ashwinkumar Subramenium G, Vijayakumar K and Pandian SK (2015). Limonene inhibits streptococcal biofilm formation by targeting surface associated virulence factors. Journal of Medical Microbiology [Society for General Microbiology] (Impact Factor: 2.266) (in press).*
   
7. 
   Nithyanand P, Beema Shafreen RM, Muthamil S and Pandian SK (2015). Usnic acid inhibits biofilm formation and virulent morphological traits of Candida albicans. Microbiological Research DOI:10.1016/j.micres.2015.06.009 [Elsevier, USA] (Impact Factor: 2.561).*
   
8. 
   Salini R and Pandian SK (2015). Interference of quorum sensing in urinary pathogen Serratia marcescens by Anethum graveolens. Pathogens and Disease [Formerly FEMS Immunology & Medical Microbiology] [Wiley-Blackwell, UK] (Impact Factor: 2.554) (Accepted).*
   
9. 
   Ashwinkumar Subramenium G, Viszwapriya D, Prasanth MI, Balamurugan K and Pandian SK (2015). covR mediated antibiofilm activity of 3-Furancarboxaldehyde increases the virulence of Group A Streptococcus. PLOS ONE [Public Library of Science] 10(5): e0127210 (Impact Factor: 3.534)*
   
10. 
    Santhakumari S, Kannappan A, Pandian SK, Thajuddin N, Rajendran RB and Ravi AV (2015). Inhibitory effect of marine cyanobacterial extract on biofilm formation and virulence factor production of bacterial pathogens causing vibriosis in aquaculture. Journal of Applied Phycology (in press) [Springer] (Impact factor: 2.492).*
    
11. 
    Nithyanand P, Beema Shafreen RM, Muthamil S, Murugan R and Pandian SK (2015) Essential oils from commercial and wild Patchouli modulate Group A Streptococcal biofilms. Industrial Crops and Products 69:180-186 [Elsevier] (Impact Factor: 3.208).*
    
12. 
    Padmavathi A, Periyasamy M and Pandian SK (2015). Assessment of 2, 4-Di-tert- butylphenol induced modifications in extracellular polymeric substances of Serratia marcescens. Bioresource Technology DOI: 10.1016/j.biortech.2015.01.049 [Elsevier] (Impact Factor: 5.039).*
    

5. 
   Salini R, Sindhulakshmi M and Pandian SK (2015). Inhibition of quorum sensing mediated biofilm development and virulence in uropathogens by Hyptis suaveolens. Antonie van Leeuwenhoek Journal of Microbiology 107:1095-1106 [Springer] (Impact Factor: 2.137).*
   
6. 
   Nithyanand P, Beema Shafreen RM, Muthamil S and Pandian SK (2015). Usnic acid, a lichen secondary metabolite inhibits Group A Streptococcus biofilms. Antonie van Leeuwenhoek. 107:263-272 [Springer] (Impact Factor: 2.137). *
   
7. 
   Padmavathi A, Abinaya A and Pandian SK (2014). Phenol, 2, 4- bis (1,1- dimethylethyl) of marine bacterial origin inhibits quorum sensing mediated biofilm formation in an uropathogen Serratia marcescens. Biofouling 30:1111-1122 [Taylor & Francis, UK] (Impact factor: 3.415)*
   
8. 
   Vinoj G, R Pati, A Sonawane, Vaseeharan B (2015). In vitro cytotoxic effects of gold nanoparticles coated with functional AHL Lactonase protein from Bacillus licheniformis and its antibiofilm activity against Proteus species. Antimicrobial agents and chemotherapy, 2: 763-771 (Impact Factor: 4.451).*
   
9. 
   Vinoj G, Jayakumar R, Chen JC, Boonsirm W, Shanthi S, Vaseeharan B (2015). N- hexanoyl-L-homoserine lactone-degrading Pseudomonas aeruginosa PsDAHP1 protects zebrafish against Vibrio parahaemolyticus infection. Fish and Shellfish Immunology, 1: 204-212 (Impact Factor: 3.034).*
   
10. 
    Vinoj G, Vaseeharan B, Thomas S, Spiers AJ, Shanthi S (2014). Quorum-Quenching Activity of the AHL-Lactonase from Bacillus licheniformis DAHB1 Inhibits Vibrio Biofilm Formation In Vitro and Reduces Shrimp Intestinal Colonisation and Mortality. Marine Biotechnology. (DOI:10.1007/s10126-014-9585-9) (Impact Factor: 2.7).*
    
11. 
    Vinoj G, Vaseeharan B, DavidJayaseelan B, Rajakumaran P, Ravi C (2013). Inhibitory effects of Bacillus licheniformis (DAB1) and Pseudomonas aeruginosa (DAP1) against Vibrio parahaemolyticus isolated from Fenneropenaeus indicus. Aquaculture International 21: 1121-1135. (Impact Factor:1.04).*
    
12. 
    Vinoj G, Vaseeharan B, G Brennan (2013). Green fluorescent protein visualization of Vibrio parahaemolyticus infections in Indian white shrimp Fenneropenaeus indicus (H Milne Edwards). Aquaculture Research 1–12. (Impact Factor: 1.42).*
    
13. 
    Meiyazhagan G, Raju R, Winfred SB, Mannivanan B, Bhoopalan H, Shankar V, Sathiya S, Deepa Parvathi V, Ravishankar P, Venkatesh babu N, Malini T, Kandaswamy D, Jeyakanthan J, Raghunathan R, Ganesh V (2015). Bioactivity Studies of β-Lactam Derived Polycyclic Fused Pyrroli-Dine/Pyrrolizidine Derivatives in Dentistry: In Vitro, In Vivo and In Silico Studies. PLOS ONE, 10(7): e0131433 (Impact Factor: 3.234).*
    
14. 
    Patil B, Sablok G, Kumar S, Singh SK, Chainy GBN (2015). Investigating the Conformational Structure and Potential Site Interactions of SOD Inhibitors on Ec- SOD in Marine Mud Crab Scylla serrata: A Molecular Modeling Approach. Interdiscipilinary ScienceS Computational Life Sciences, 7: 1-7 (Impact Factor: 0.66).*
    
15. 
    Sivakamavalli J, Selvaraj C, Singh SK, & Vaseeharan B (2015). Molecular cloning, relative expression and structural analysis of pattern recognition molecule β-glucan binding protein from mangrove crab Episesarma tetragonum. Biotechnology and Applied Biochemistry, 3:416-23 (Impact Factor: 1.362).*
    
16. 
    Karthikeyan M, Shridevi V, Rajiv Rose, Anandan B, Singh KhD, Shanmugasundaram S, Mohan D, Ramesh, Jayaraman G (2013). Angiotensin Gene Polymorphisms (T174M and M235T) are Significantly Associated with the Hypertensive Patients of Tamil Nadu, South India. International Journal of Human Genetics 4: 201-207 (Impact Factor:0.37).*
    

5. 
   Singh KD, Karthikeyan M (2013). Molecular Modeling, Quantum Polarized Ligand Docking and Structure Based 3D-QSAR study of imidazole series as Dual Endothelin and Angiotensin II Receptor Antagonists. Acta Pharmacologica Sinica (NPG), 34:1592-1606, (Impact Factor: 2.912).*
   

Budget Summary

S.No.	Items	Alagappa Univ		Annamalai Univ		Bharathidasan Univ		Bharathiyar Univ		Madurai Kamaraj Univ		Manonmaniam Sundaranar Univ		Periyar Univ		Univ of Madras		Total (in Lakhs)
A) Non-recurring
		Year I	Year II	Year I	Year II	Year I	Year II	Year I	Year II	Year I	Year II	Year I	Year II	Year I	Year II	Year I	Year II
1	Equipment	735	0	224	0	395	0	234	0	105	0	87	0	30	0	532	0	2342
Total (A)		735	0	224	0	395	0	234	0	105	0	87	0	30	0	532	0	2342
B) Recurring
1	Manpower	46.08	46.08	11.52	11.52	19.2	19.2	15.36	15.36	19.2	19.2	5.76	5.76	5.76	5.76	15.36	15.36	276.48
2	Contingencies	150	150	70	50	50	50	40	40	100	100	30	10	6	4.5	10	10	870.5
3	Travel	9.42	9.42	4.076	3.076	3.46	3.46	2.768	2.768	5.96	5.96	1.785	0.785	0.588	0.513	1.265	1.265	56.569
4	Overhead (5%)	9.42	9.42	4.076	3.076	3.46	3.46	2.768	2.768	5.96	5.96	1.785	0.785	0.588	0.513	1.265	1.265	56.569
Total (B)		214.92	214.92	89.672	67.67	76.12	76.12	60.896	60.896	131.12	131.12	39.33	17.33	12.936	11.29	27.89	27.89	1260.118
Total (A+B)		949.92	214.92	313.67	67.67	471.12	76.12	294.896	60.896	236.12	131.12	126.33	17.33	42.936	11.29	559.89	27.89	3602.118

Budget requested for Year I	Rs. in Lakhs 2994.884
Budget requested for Year II	607.234
Grand Total	3602.118

34

58

Budget for Alagappa University

S.No	Items	Year I	Year II	Total (inRs)
A) Non-recurring
1	Nano LC-MS/MS	22000000		22000000
2	Multilabel Reader, GC-MS	5000000		5000000
3	Next Generation Sequencer	20000000		20000000
4	DIGE Typhoon Scanner and Decyder software	10000000		10000000
5	Millipore Water Purification System, Flow cells, HPLC columns, Micro centrifuges, Icemaker, Thermal Cyclers, Laminar Air Flow Chambers, Deep Freezers, Gel Docs ,	5000000		5000000
6	Isothermal Titration Calorimetry	6000000		6000000
7	Ultra Centrifuge	3000000		3000000
8	Fluorescence Spectrometer	2500000		2500000
Total (A)		73500000		73500000
B) Recurring
1	Manpower (Project Fellow) Rs 16000 per month; 24 Numbers	4608000	4608000	9216000
2	Contingency	15000000	15000000	30000000
3	Travel	942000	942000	1884000
4	Overhead (5% of Recurring Expenditure)	942000	942000	1884000
Total (B)		21492000	21492000	42984000
Total (A+B)				116484000

Budget for Annamalai University

S.No	Items	Year I	Year II	Total (in Rs)
A) Non-recurring
1	HPTLC, GC Mass/LC Mass, Deep Freezer, Lyophilizer, Stereotaxic Instrument	22400000		22400000
Total (A)		22400000		22400000
B) Recurring
1	Manpower (Project Fellow) Rs 16000 per month; 6 Numbers	1152000	1152000	2304000
2	Contingency (Inclusive of Discovery Studio 4.5/Schrodinger)	7000000	5000000	12000000
3	Travel	407600	307600	715200
4	Overhead (5% of Recurring Expenditure)	407600	307600	715200
Total (B)		8967200	6767200	15734400
Total (A+B)				38134400

Budget for Bharathidasan University

S.No	Items	Year I	Year II	Total (inRs)
A) Non-recurring
1	Liquid Nitrogen Plant	15000000		15000000
2	Fluorescent Activated Cell Sorter	7500000		7500000
3	Preparative HPLC	2500000		2500000
4	Freezing Microtome	1500000		1500000
5	Walk in Cold Room	2000000		2000000
6	Lyophilizer	1000000		1000000
7	Dynamic Light Scattering	2000000		2000000
8	Flash Column Chrometography	3000000		3000000
9	Fermentor	3500000		3500000
10	FTIR	1500000		1500000
Total (A)		39500000		39500000
B) Recurring
1	Manpower (Project Fellow) Rs 16000 per month; 10 Numbers	1920000	1920000	3840000
2	Contingency	5000000	5000000	10000000
3	Travel	346000	346000	692000
4	Overhead (5% of Recurring Expenditure)	346000	346000	692000
Total (B)		7612000	7612000	15224000
Total (A+B)				54724000

Budget for Bharathiyar University

S.No	Items	Year I	Year II	Total (inRs)
A) Non-recurring
1	Bio-safety hood	1000000		1000000
2	Fully Automated Karyotyping Software	6000000		6000000
3	Real Time PCR	1400000		1400000
4	Inverted and Fluorescence Microscope	2500000		2500000
5	^CO2 ^Incubator	800000		800000
6	-80° Deep Freezer	700000		700000
7	Millipore Water	1500000		1500000
8	HPLC	1200000		1200000
9	UV Spectrophotometer	700000		700000
10	Nanodrop	900000		900000
11	2D gel electrophoresis	2500000		2500000
12	Cooling Centrifuge	700000		700000
13	Freeze Dryer	1500000		1500000
14	Plant Growth Chamber	1000000		1000000
15	20 KVA UPS	1000000		1000000
Total (A)		23400000		23400000
B) Recurring
1	Manpower (Project Fellow) Rs 16000 per month; 8 Numbers	1536000	1536000	3072000
2	Contingency	4000000	4000000	8000000
3	Travel	276800	276800	553600
4	Overhead (5% of Recurring Expenditure)	276800	276800	553600
Total (B)		6089600	6089600	12179200
Total (A+B)				35579200

Budget for Madurai Kamaraj University

S.No	Items	Year I	Year II	Total (inRs)
A) Non-recurring
1	Desktop Computers with WiFi (60 Numbers)	3000000		3000000
2	Multimode Cell Imaging & Assay System	7500000		7500000
Total (A)		10500000		10500000
B) Recurring
1	Manpower (Project Fellow) Rs 16000 per month; 10 Numbers	1920000	1920000	3840000
2	Contingency	10000000	10000000	20000000
3	Travel	596000	596000	1192000
4	Overhead (5% of Recurring Expenditure)	596000	596000	1192000
Total (B)		13112000	13112000	26224000
Total (A+B)				36724000

Budget for Manonmaniam Sundaranar University

S.No	Items	Year I	Year II	Total (inRs)
A) Non-recurring
1	High speed centrifuge	1200000		1200000
2	Fermentor with computer accessories	2500000		2500000
3	Lyophilizer	1500000		1500000
4	Preparative HPLC	1500000		1500000
5	Real Time PCR	2000000		2000000
Total (A)		8700000		8700000
B) Recurring
1	Manpower (Project Fellow) Rs 16000 per month; 3 Numbers	576000	576000	1152000
2	Contingency (Chemicals/Glasswares/Renovation and upgradation of Animal House)	3000000	1000000	4000000
3	Travel	178800	78800	257600
4	Overhead (5% of Recurring Expenditure)	178800	78800	257600
Total (B)		3933600	1733600	5667200
Total (A+B)				14367200

Budget for Periyar University

S.No	Items	Year I	Year II	Total (inRs)
A) Non-recurring
1	HPLC	1800000		1800000
2	FTIR	1200000		1200000
Total (A)		3000000		3000000
B) Recurring
1	Manpower (Project Fellow) Rs 16000 per month; 3 Numbers	576000	576000	1152000
2	Contingency	600000	450000	1050000
3	Travel	58800	51300	110100
4	Overhead (5% of Recurring Expenditure)	58800	51300	110100
Total (B)		1293600	1128600	2422200
Total (A+B)				5422200

Budget for University of Madras

S.No	Items	Year I	Year II	Total (inRs)
A) Non-recurring
1	IR-NIR Imaging Microscope	15000000		15000000
2	RAMAN imaging system with 4 laser line	18000000		18000000
3	Electrospinning Unit	3000000		3000000
5	Microwave Digestion system	2000000		2000000
6	ESR Spectrometer	13000000		13000000
7	Real Time PCR	1500000		1500000
8	80 Deep freezer	700000		700000
Total (A)		53200000		53200000
B) Recurring
1	Manpower (Project Fellow) Rs 16000 per month; 8Numbers	1536000	1536000	3072000
2	Contingency	1000000	1000000	2000000
3	Travel	126800	126800	253600
4	Overhead (5% of Recurring Expenditure)	126800	126800	253600
Total (B)		2789600	2789600	5579200
		55989600	2789600
Total (A+B)				58779200

Annexure I

Preliminary work done/Leads already obtained relevant to the focus area

• 
  List of identified anti-QS and anti-biofilmagents
  

• 
  Antibacterial activity of the candidate lead compound(s)
  

• 
  Antibiofilm activity of the candidate lead compound(s)
  

• 
  Purification/Characterization of candidate lead compound(s)
  

• 
  Proteomics analysis to identify the mode of action
  

• 
  In vivo analysis using model systems (C. elagans)
  

•