Dr lalji singh
B. ACADEMIC AND SERVICE AFFILIATIONS
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B. ACADEMIC AND SERVICE AFFILIATIONS
C. Research Interests 1. Molecular Basis of Sex Determination 2. DNA Fingerprinting 3. Wildlife Conservation 4. Human Genome Studies 5. Ancient DNA Studies
1. Shirly George Panicker Isolation and characterization of 1994 Bkm-satellite DNA from snake (Bungarus fasciatus) and a Bkm- association Y chromosome- specific clone M34 from mouse 
2. K R Rajyashri Molecular mechanism of sex- 1994 determination: Isolation and characterization of an unusually conserved testis determining gene
3. Raghavender Nagaraj Studies on sex and tissue-specific 1995 Bkm-binding protein in mouse
4. Narendra Pathak Molecular characterization of 1998 gene(s) expressed specifically in snake oocytes
5. Sanjay Sachdev Molecular analysis of Bkm- 1998 associated DNA sequences in Drosophila. ___________________________________________________________________ 6. Anju Singh Identification and Characterization 2000 of a Highly Conserved Gene, which is Predominantly Expressed in the Human, Testis, in Silkworm Bombyx mori ___________________________________________________________________ 7. S. Subramanian Molecular Analysis of Y Chromo- 2003 some DNA in Human 
Of tiger Panthera tigris tigris ________________________________________________________________________ 9. V V Suryanarayana Molecular status of detoxification 2008 genes and functional Proteomics in recurrent early pregnancy loss __________________________________________________________________ Postdoctoral Fellows worked/working with Dr Lalji Singh
4. Dr Sokka Reddy 5. Dr Qurratulain Hasan 6. Dr Saswati Ghosh 7. Dr C S Shashikant 8. Dr Vanya Shah 9. Dr Sanjeev Shangary 10 Dr Bhupendra Narain Singh 11. Dr Divyendu Saha 12. Dr Moinak Banerjee
D. Research Papers Published (1968-2008) 
1. Singh, L., Sharma, T. and Ray-Chaudhuri, S.P.: W. chromosome in the Indian water snake (checkered keel back) Natrix piscator (Colubridae). Experientia, (Basel) 24, 79-80 (1968). 2. Singh, L., Sharma, T. and Ray-Chaudhuri, S.P.: Chromosomes and classification of the snakes of the family Boidae. Cytogenetics, 7, 161-168 (1968). 3. Ray-Chaudhuri, S.P., Sharma, T., Pathak, S. and Singh, L.: Chromosomes and the karyotype of the Pangolin, Manis pentadactyla L. (Pholidota Mammalia). Experientia, (Basel) 26, 1167-1168 (1969). 4. Ray-Chaudhuri, S.P., Singh, L. and Sharma, T.: Sexual dimorphism in somatic interphase nuclei of snakes. Cytogenetics, 9, 410-423 (1970). 5. Singh, L., Sharma, T. and Ray-Chaudhuri, S.P.: Multiple sex chromosomes in the common Indian krait, Bungarus caeruleus Schneider. Chromosoma (Berl.), 31, 386-391 (1970). 6. Singh, L., Sharma, T. and Ray-Chaudhuri, S.P.: Chromosome numbers and sex chromosomes in few Indian species of amphibian and reptiles. Mammal. Chrom. Newslett. 11, 91-94 (1970). 7. Ray-Chaudhuri, S.P., Singh, L. and Sharma, T.: Evolution of sex chromosomes and formation of W-chromatin in snakes. Chromosoma (Berl.), 33, 239-251 (1971). 8. Singh, L.: Multiple W chromosome in a sea snake, Enhydrina schistosa Daudin. Experientia (Basel), 28, 95-97 (1972). 9. Ray-Chaudhuri, S.P. and Singh, L.: DNA replication pattern in sex chromosome of snakes. Nucleus (Calcutta), 15, 200-210 (1972). 10. Singh, L.: Evolution of karyotypes in snakes. Chromosoma (Berl.), 38, 185-236 (1972). 11. Singh, L.: Karyological study of three species of turtles from India. Indian Biologist, 4, 64-69 (1972). 12. Singh, L. and Ray-Chaudhuri, S.P.: DNA replication pattern in the chromosomes of Crocodylus palustris (Lesson). Nucleus (Calcutta), 16, 33-37 (1973). 13. Singh, L.: The present status of sex chromosomes in amphibians. Nucleus (Calcutta), 17, 17-27 (1974). 14. Singh, L.: Study of mitotic and meiotic chromosomes in seven species of lizards. Proc. Zool. Soc., Calcutta, 27, 57-79 (1974). 15. Singh, L.: Chromosomes of six species of Indian snakes. Herpetologica, 30, 419-429 (1974). 16. Gupta, P., Singh, L. and Ray-Chaudhuri, S.P.: Chromosomes of Indian breeds of cattle. Nucleus (Calcutta), 17, 129-132 (1974). 17. Singh, L. and Ray-Chaudhuri, S.P.: Localization of C-band in the W sex chromosome of Common Indian krait, Bungarus caeruleus Schneider. Nucleus (Calcutta), 18, 163-166 (1975). 18. Singh, L., Purdom, I.F. and Jones, K.W.: The chromosomal localization of satellite DNA in Ptyas mucosus (Ophidia), Colubridae). Chromosoma (Berl.), 57, 177-184 (1976). 19. Singh, L., Purdom, I.F. and Jones, K.W.: Satellite DNA and evolution of sex chromosomes. Chromosoma (Berl.), 59, 43-62 (1976). 20. Singh, L., Purdom, I.F. and Jones, K.W.: Effect of different denaturing agents on the detectability of specific DNA sequences of various base compositions by in situ hybridization. Chromosoma (Berl.), 60, 377-389 (1977). 21. Singh, L., Purdom, I.F. and Jones, K.W.: Behaviour of sex chromosomes associated satellite DNAs in somatic and germ cells in snakes. Chromosoma (Berl.), 71, 167-181 (1979). 22. Singh, L., Ray-Chaudhuri, S.P., Majumdar, K., Purdom, I.F. and Jones, K.W.: Sex specific chromosome polymorphisms in the Common Indian krait, Bungarus caeruleus schneider (Ophidia, Elapidae). Chromosoma (Berl.), 73, 93-108 (1979). 23. Singh, L., Purdom, I.F. and Jones, K.W.: Sex chromosome associated satellite DNA: Evolution and conservation. Chromosoma (Berl.), 79, 137-157 (1980). 24. Singh, L., Purdom, I.F. and Jones, K.W.: Conserved sex chromosome-associated nucleotide sequences in eukaryotes. Cold Spring Harbor Symp. Quant. Biol., 45, 805-813 (1981). 25. Jones, K.W. and Singh, L.: Conserved repeated DNA sequences in vertebrate sex chromosomes. Hum. Genet. 58, 46-53 (1981). 26. Jones, K.W. and Singh, L.: Conserved sex-associated repeated DNA in vertebrates. In: “Genome Evolution” (Eds. G. Dover and R. Flavell), Academic Press, London, pp.135-154 (1982). 27. Singh, L. and Jones, K.W.: Sex reversal (Sxr) in the mouse (Mus musculus) is caused by a recurrent non-reciprocal crossover involving the X and an aberrant Y chromosome. Cell 28, 205-216 (1982). 28. Muller, U., Singh, L. Grund, S. and Jones, K.W.: Ovarian cells participate in the formation of tubular structures in mouse/rat heterosexual gonadal cocultures. Differentiation, 22, 136-138 (1982). 29. Jones, K.W., Singh, L. and Phillips, C.: Conserved Nucleotide sequences on sex chromosomes. Proc. John Inn’s Symp. 265-287 (1983). 30. Singh, L., Phillips, C. and Jones, K.W.: The conserved nucleotide sequences of Bkm, including those, which define Sxr in the mouse, are transcribed. Cell 36, 111-120 (1984). 31. Singh, L. and Jones, K.W. : The use of heparin as a simple cost-effective means of controlling background in nucleic acid hybridization procedures. Nucl. Acids Res., 8, 5627-5637 (1984). 32. Jones, K.W. and Singh, L.: Snakes and the evolution of sex chromosomes. Trends Genet. 1, 55-61 (1985). 33. Singh, L. and Jones, K.W.: Bkm sequences are polymorphic in humans and are clustered in pericentric regions of various acrocentric chromosomes including the Y. Hum. Genet. 73, 304-308 (1986). 34. Singh, L., Matsukuma, S. and Jones, K.W.: Testis development in a mouse with 10% of XY cells. Dev. Biol., 122, 287-290 (1987). 35. Jones, K.W., Singh, L. and Edwards, R.G.: The use of probes for the Y chromosome in preimplantation embryo cells. Hum. Reprod. 2, 439-445 (1987). 36 Jones, K.W., Olszewska, E., and Singh, L.: Rapidly evolving Bkm DNA is associated with hypervariable domains. In: “Chromosomes Today”, 9, 22-29. (Eds. A. Stahl, J.M. Luciani & A.M. Vagner - Capodano. Allen & Unwin Suffolk, G.B.) (1987). 37. Matsukuma, S., Singh, L., and Jones, K.W.: Microinjection of Bkm related male specific mouse DNA into autologous zygotes. J. Genet. 66, 163-176 (1987). 38. Singh, L., Matsukuma, S., and Jones, K.W.: The use of Y chromosome specific repeated DNA sequences in the analysis of testis development in an XX/XY mouse. Development, 101 (supplement), 143-150 (1987). 39. Singh, L., Winking, H., Jones, K.W., and Gropp, A.: Restriction fragment polymorphism in the sex-determining region of the Y chromosomal DNA of European wild mice. Mol. Gen. Genet. 212, 440-449 (1988). 40. Singh, L.: DNA profiling and its applications. Curr. Sci., 60, 580-585 (1991). 41. Aggarwal, R.K., Lang, J.W., Singh, L.: Isolation of high molecular weight DNA from small samples of blood having nucleated erythrocytes, collected, transported and stored at room temperature. Genetic Analysis: Techniques and Applications, 9(2), 54-57 (1992). 42. Singh, L. and Majumdar, K.C.: Striking similarity in molecular organization of sex chromosomes is a reflection of their common mode of action. In: "Sex Chromosomes and Sex-determining Genes" (Eds. Ken C Reed and Jennifer A Marshall Graves), pp. 337-356 (1993). 43. Lang, J.W., Aggarwal, R.K., Majumdar, K.C. and Singh, L.: Individualisation and estimation of relatedness in crocodilians by DNA fingerprinting with Bkm derived probe. Mol. Gen. Genet. 238, 49-58 (1993). 44. Bhargava, P.M. and Singh, L.: What should India be doing on the human genome? Curr. Sci., 65(9), 663-664 (1993). 45. Panicker, S.G. and Singh, L.: Banded krait minor satellite (Bkm) contains sex and species-specific repetitive DNA. Chromosoma, 103, 40-45 (1994). 46. Singh, L., Panicker, S.G., Nagaraj, R. and Majumdar, K.C.: Banded krait minor-satellite (Bkm)-associated Y chromosome-specific repetitive DNA in mouse. Nucl. Acids Res., 22(12), 2289-2295 (1994). 47. Aggarwal, R.K., Majumdar, K.C., Lang, J.W. and Singh, L.: Generic affinities among crocodilians as revealed by DNA fingerprinting using a Bkm-derived probe. Proc. Natl. Acad. Sci., (USA) 91, 10601-10605 (1994). 48. Singh, L. and Majumdar, K.C.: Behaviour of microchromosome associated satellite DNA in Bungarus fasciatus (Ophidia, Elapidae). J. Genet. 73(1),1-15 (1994). 49. Singh, L., Wadhwa, R., Naidu, S., Nagaraj, R. and Ganesan, M.: Sex and tissue-specific Bkm (GATA)-binding Protein in the Germ Cells of heterogametic sex. J. Biol. Chem., 269, 25321-25327 (1994). 50. Singh, L., Panicker, S.G., Nagaraj, R. and Majumdar, K.C.: Functional Significance of Molecular organization of sex chromosomes. Proc. Indian Natl. Sci. Acad., 360, 455-470 (1994). 51. Aggarwal, R.K., Singh, P. and Singh, L.: Genetic fingerprinting: an overview with special reference to its application in agriculture. In: Proc. Golden Jubilee Symp. Genetic Research and Education: Current Trends and Next Fifty Years. (Eds. B. Sharma et al.), Ind. Soc. Genet. & Plant Breeding, 4, pp: 1909-1925 (1995). 52. Singh, L., and Rajyashri, K.R.: Molecular basis of sex determination and differentiation in mammals. Molecular Genetics & Gene Therapy: The New Frontier, Scientific Communications, Amsterdam, pp 21-44 (1995). 53. Singh, L.: Biological significance of minisatellites. Electrophoresis, 16, 1586-1595 (1995). 54. Nagaraju, J., Sharma, A., Sethuraman, B. N., Rao, G. V. and Singh, L.: DNA fingerprinting in silkworm Bombyx mori using banded krait minor satellite DNA-derived probe. Electrophoresis, 16, 1639-1642 (1995). 55. Rajyashri, K.R. and Singh, L.: A Bkm-associated human Y-chromosomal DNA is conserved and transcribed in the testis of mouse. Chromosoma, 104, 274-281 (1995). 56. Majumdar, K.C., Shetty, S., Wadhwa, R., Bhaskar, S., Ganesan, M. and Singh, L.: Detection and purification of sequence specific DNA binding protein. Anal. Biochem., 241, 23-29 (1996). 57. Jobling, M.A., Samara, V., Pandya, A., Fretwell, N., Bernasconi, B., Mitchell, R.J., Gerelsaikhan, T., Dashnyam, B., Sajantila, A., Salo, P.J., Nakahori, Y., Disteche, C.M., Thangaraj, K., Singh, L., Crawford, M.H. and Tyler-Smith, C.: Recurrent duplication and deletion polymorphisms on the long arm of the Y chromosome in normal males. Hum. Mol. Gen., 5, 1767-1775 (1996). 58. Nagaraju, J.G. and Singh, L.: Assessment of genetic diversity by DNA profiling and its significance in improvement of silkworm, Bombyx mori. Electrophoresis, 18, 1676-1681 (1997). 59. Shankaranarayanan, P., Banerjee, M., Kackar, R.K., Aggarwal, R.K. and Singh, L.: Genetic variation in Asiatic lions and Indian tigers. Electrophoresis, 18, 1693-1700 (1997). 60. Thangaraj, K., Nalini J Gupta, Chakravarty, B., and Singh, L.: XXY Female: An unique case of sex reversal. THE LANCET, 352 (9134), 1121 (1998). 61. Shankaranarayanan, P. and Singh, L.: A rapid and simplified protocol for isolation of DNA from Scat samples. Curr. Sci., 75 (9), 883-884 (1998). 62. Shankaranarayanan, P. and Singh, L.: Mitochondrial DNA sequences divergence among big cats and their hybrids. Curr. Sci., 75(9), 919-923 (1998). 63. Vanaja, D.K., Sivakumar, B., Rachel, A.J., Singh, L., Janardhanasarma, M.K. and Habibullah, C.M.: In vivo identification, survival and functional efficacy of transplanted hepatocytes in acute liver failure mice model by FISH using Y-chromosome probe. Cell Transplantation 7(3), 267-273 (1998). 64. Arpita Pandya, Turi E. King, Fabricio R. Santos, Paul G. Taylor, Kumarasamy Thangaraj, Lalji Singh, Mark A. Jobling and Chris Tyler-Smith: A polymorphic human Y-chromosomal G to A transition found in India. Ind. J. Hum. Genet. 4, 52-61 (1998). 65. Parmar, S.N.S., Singh, L., Rao, G.V., Mishra, S.K. and Reddy, A.R. DNA fingerprinting in poultry using multilocus probe Bkm-2(8). Indian Veterinary Journal, 75, 371-374 (1998). 66. Singh, L.: DNA fingerprinting technology and emerging legal and ethical issues. In: Human Genome Research: Emerging Ethical, Legal, Social and Economic Issues (Eds. M G K Menon, P N Tandon, S S Agarwal and V P Sharma), pp.116-135 (1999). 67. Siva Kumar, S., Subramanian, V., Walimbe, S.R. and Singh, L.: Current Trends in 'Ancient DNA studies' - A Review. Curr. Sci., 76(7), 101-107 (1999). 68. Narendra Kumar, A., Chandak, G.R., Rajasekhar, A., Reddy, N.C.K., and Singh, L.: Fetus-in-Fetu: A case report with molecular analysis. J. Ped. Surg., 34 (4), 1-5 (1999). 69. Niranjan, Y., Chandak, G.R., Veerraju, P and Singh, L.: Some Atypical and Rare Sickle Gene Haplotypes in Populations of Andhra Pradesh, India. Hum. Biol., 71 (3), 335-342 (1999). 70. Sharma, A., Niphadkar, M.P., Kathirvel, P., Nagaraju, J. and Singh, L.: DNA fingerprint variability within and among the Silkworm Bombyx mori genotypes and estimation of their genetic relatedness using Bkm-derived probe. J. Hered. 90, 315-319 (1999). 71. Thangaraj, K., Ramana, G.V. and Singh, L.: Y-chromosome and mitochondrial DNA polymorphisms in Indian populations. Electrophoresis, 20, 1743-1747 (1999). 72. Hurles, M.E., Veitia, R., Arroyo, E., Armenteros, M., Betranpetit, J., Perez-Lezaun, A., Bosch, E., Shlumukova, M., Cambon-Thomsen, A., McElreavey, K., Lopez de Munian, A., Rohl, A., Wilson, I.J., Singh, L., Pandya, A., Santos, F.R., Tyler-Smith, C. and Jobling, M.A.: Recent male-mediated gene flow over a linguistic barrier in Iberia suggested by analysis of a Y-chromosomal DNA polymorphism. American Journal of Human Genetics, 65, 1437-1448 (1999). 73. Singh, L., Pathak, N.H., Rachel, A.J. and Thangaraj, K.: Snake's Eye View of Adam and Eve in Reproductive Immunology (Ed. Satish K Gupta) pp. 132-148 (1999). 74. Tatiana Zerjal, Arpita Pasndya, Fabricio R. Santos, Raju Adhikari, Eduardo Tarazona, Lars Beckman, Manfred Kayser, Oleg Evgrafov, Lalji Singh, Kumaraswamy Thangaraj, Giovanni Destro-Bisol, Mark G. Thomas, Rahell Qamar, Qasim Mehdi, Zoe H. Rosser, Matt E. Hurles, Mark A. Jobling and Chris Tyler-Smith: The use of Y-chromosomal DNA variation to investigate population history: recent male spread in Asia and Europe. In: Genomic Diversity: Applications in Human Population Genetics (Ed. Papiha, Deka and Chakraborty) pp.91-101 (1999). 75. Hema, M.G., Zeenath, J., Ahuja, Y.R., Singh, L. Analysis of SRY : the testis determining factor in individuals with 46XY gonadal dysgenesis. Medical Science Research, 27, 95-97 (1999).
78. Anil K Mandal, Amar Pal Singh, Laxmi Rao, Lalji Singh, Stella Hornby and Barry Jones: Robert’s Pseudothalidomide Syndrome. Arch Ophthalmol 118, 1462-1463 (2000). 79. P. K. Rout, K. Thangaraj, A Mandal, L Singh: DNA Finger Printing of Indian goats with a BKM-derived probe. 7th International Conference on Goats, France, 15-21 May 2000. 80. G.V. Ramana, L.Singh, R Chakraborty: The SRY-1532 site of the Human Y chromosome is subject to recurrent single nucleotide mutations. Human Biology, 73, 71-80, (2001). 81. G.V. Ramana, Bing Su, Li Jin, Lalji Singh, Ning Wang, Peter Underhill and Ranajit Chakraborty: Y-chromosome SNP haplotypes suggest evidence of gene flow among caste, tribe, and the migrant Siddi populations of Andhra Pradesh, South India. European J. Hum. Genetics, 9, 695-700 (2001). 82. Thangaraj, K., Reddy, A.G. and Singh, L. Is amelogenin gene reliable for gender identification in forensic casework and prenatal diagnosis? Intl. J. Legal Med., 116, 121-123 (2002). 83. GR Chandak, MM Idris, DN Reddy, S Bhaskar, PVJ Sriram, L Singh. “Mutations in pancreatic secretory trypsin inhibitor gene (PSTI/SPINK1) rather than cationic trypsinogen gene (PRSS1) are significantly associated with tropical calcific pancreatitis.” J Med. Genet., 39, 347-351 (2002). 84. David B. Everman, Cynthai F. Bartels, Yue Yang, Niranjan Yanamandra, Frances R. Goodman, J. Roberto Mendoza-Londono, Ravi Savarirayan, Susan M. White, John M. Graham Jr., Robert Peter Gale, Eva Svarch, William G. Newman, Albert R. Kleckers, Clair A. Francomano, Vinukonda Govindaiah, Lalji Singh, Stuart Morrison, J. Terrig Thomas and Mathew L. Warman. “The mutational spectrum of brachydactyly type C”. American Journal of Medical Genetics, 112, 291-296 (2002). 85. R. K. Aggarwal, V V Shenoy, J. Ramadevi, R. Rajkumar, L. Singh. Molecular characterization of some Indian Basmati and other elite rice genotypes using fluorescent-AFLP. Thero Appl Genet., 105, 680-690 (2002). 86. K. Thangaraj, M. Joshi, A.G. Reddy, N.J. Gupta, B. Chakravarty, L. Singh: CAG repeat expansion in the androgen receptor gene is not associated with male infertility in Indian Populations. J. Andrology, 23, 815-818 (2002). 87. A. Singh, K. Shaijala, A. Gaur and L. Singh: Development and characterization of novel microsatellite markers in the Asiatic lion (Panthera leo persica). Molecular Ecology Notes, 2, 542-543 (2002). 88. G.R. Chandak, M. Uma Sridevi, C.J. Vas, D.M. Panikker and L. Singh: Apolipoprotein E and Presenilin-1 Allelic Variation and Alzheimer’s Disease in India. Human Biology, v.74, no.5, pp. 683-693 (2002). 89. Subbaya Subramanian, Vamsi M Madgula, Ranjan George, Rakesh K Mishra, Madhusudhan W Pandit, Chandrashekar S Kumar and Lalji Singh: MRD: a microsatellite repeats database for prokaryotic and eukaryotic genomes. Genome Biology 3, 11.1-11.13 (2002). 90. Christopher N. Balakrishnan, Steven L. Monfort, Ajay Gaur, Lalji Singh and Michael D. Sorenson: Phylogeography and conservation genetics of Eld’s deer (Cervus eldi). Molecular Ecology, 12, 1-10 (2003). 91. Thangaraj, K., Subramanian S., Reddy A.G., Singh L.: Unique Case of Deletion and Duplication in the Long Arm of the Ychromosome in an Individual with Ambiguous Genitalia. Am. J. Med. Genet., 116A, 205-207 (2003). 92. Kumarasamy Thangaraj, Lalji Singh, Alla G. Reddy, V Raghavendra Rao, Subhash C Sehgal, Peter A Underhill, Melanie Pierson, Ian G.Frames and Erika Hagelberg: Genetic Affinities of the Andaman Islanders, a Vasnishing Human Population. Current Biology, 13, 68-93 (2003) 93. Subbaya Subramanian, Rakesh K Mishra and Lalji Singh: Genome-wide analysis of microsatellite repeats in human: Abundance and their density in specific genomic regions. Genome Biology, 4, R13.1-R1310 (2003). 94. S.K. Verma and L. Singh: Novel universal primers establish identity of an enormous number of animal species for forensic application. Molecular Ecology Notes, 3,28-31 (2003). 95. Priyadarshini, P., Murthy, B. S., Nagaraju, J., Singh, L.: A GATA-binding protein expressed predominantly in the pupal ovary of the silkworm, Bombyx mori. Insect Biochemistry & Molecular Biology, 33, 185-195 (2003) 96. Subbaya Subramanian, Vamsi M Madgula, Ranjan George, Rakesh K Mishra, Madhusudhan W Pandit, Chandrashekar S Kumar and Lalji Singh: Triplet repeats in human genome: distribution and their association with genes and other genomic regions. Bioinformatics,19, 549-552 (2003). 97. Subbaya Subramanian, Rakesh K Mishra and Lalji Singh: Genome-wide analysis of Bkm sequences (GATA repeats): predominant association with sex chromosomes and potential role in higher order chromatin organization and function. Bioinformatics, 19, 6, 681-685 (2003). 98. Kumarasamy Thangaraj, Manjunath B. Joshi, Alla G. Reddy, Avinash A Rasalkar, and Lalji Singh: Sperm Mitochondrial Mutations as a Cause of Low Sperm Motility. Journal of Andrology, 24, No.3, 388-392 (2003) 99. Bhupendra N. Singh, Amritha Suresh, Gogineni Uma Prasad, Subbaya Subramanian, Mehar Sultana, Sandeep Goel, Satish Kumar and Lalji Singh: A highly conserved human gene encoding a novel member of WD-repeat family of proteins (WDR13), Genomics, 81, 315-328 (2003). 100. Kumarasamy Thangaraj, Nalini J Gupta, K Pavani, Alla G. Reddy, Subbaya Subramanian, Deepa Selvi Rani, Bibaswan Ghosh, Baidyanath Chakravarty and Lalji Singh: Y Chromosome Deletions in Azoospermic Men in India. Journal of Andrology, 24, 588-597 (2003). 101. Subramanian S, Vamsi M Madugula, Ranjan George, Satish Kumar, Madhusudhan W Pandit and Lalji Singh: SSRD: simple sequence repeat database for human genome. Comp Funct Genom., 4, 342-345 (2003). 102. Shivaji, S., Kholkute, S.D., Verma, S. K., and Lalji Singh (19 authors). Conservation of wild animals by assisted reproduction and molecular marker technology. Indian Journal of Experimental Biology, 2003, 41, 710-723. 103. Verma, S.K., Prasad K., Nagesh, N., Sultana, M., Singh, L. Was elusive carnivore a panther? DNA typing of faeces reveals the mystery. Forensic Science International, 137, 16-20 (2003). 104. A. Gaur, A. Singh, V. Arunabala, G. Umapathy, K Shailaja and L. Singh. Development and characterization of 10 novel microsatellite markers from Chital deer (Cervus axis) and their cross-amplification in other related species. Molecular Ecology Notes, 3, 607-609 (2003). 105. MW Pandit and Lalji Singh. Science of establishing identity – Past, present and future. Palaeobotanist, 52, 1-11 (2003). 106. Raghavendra V Rao, Kumarasamy Thangaraj, Alla G Reddy, V Sridhar, Lalji Singh. Pairwise MtDNA-HVRII sequence differences and geographic maternal distances among Korku, an Austro-Asiatic tribe in Central India. Indian Journal of Human Genetics, 9, 25-28 (2003). 107. Aggarwal, R. K., J. Ramadevi, L. Singh). Ancient origin and evolution of the Indian wolf: evidence from mitochondrial DNA typing of wolves from Trans-Himalayan region and Pennisular India. Genome Biology, 4: P6 (2003). 108. Lakshmi Rao, Arvind Babu, Murthy Kanakavalli, Venkata Padmalatha, Amar Pal Singh, Prashant Kumar Singh, Mamata Deenadayal and Lalji Singh. Chromosomal abnormalities and Y-chromosome microdeletions in infertile men with varicocele and idiopathic infertility of South Indian origin. Journal of Andrology, 25, 147-153 (2004) 109. Kumarasamy Thangaraj, Alla G Reddy and Lalji Singh. Mutation in STR Locus D21S11 of Father Causing Allele Mismatch in the Child. Journal of Forensic Science, 49, (2004) 110. Manjunatha B Joshi, Pramod K Rout, Ajoy K Mandal, Chris Tyler-Smith, Lalji Singh and Kumarasamy Thangaraj. Phylogeography and Origin of Indian Domestic Goats. Molecular Biology and Evolution, 21(3), 454-462 (2004). 111. Ramesh K Aggarwal, T P Velavan, D Udaykumar, P S Hendre, Kartik Shanker, B C Choudhury and Lalji Singh. Development and characterization of novel microsatellite markers from the olive ridley sea turtle (Lepidochelys olivacea). Molecular Ecology Notes, 4, 77-79 (2004). 112. Anju Singh, Ajay Gaur, K. Shailaja, B. Satyare Bala, Lalji Singh. A novel microsatellite (STR) marker for forensic identification of big cats in India. Forensic Science International, 141, 143-147 (2004). 113. G.R. Chandak, M.M. Idris, D.N. Reddy, K.R. Mani, S. Bhaskar, G.V. Rao, L. Singh. Absence of PRSS1 mutations and association of SPINK1 trypsin inhibitor mutations in hereditary and non-heriditary chronic pancreatitis. Gut, 53, 723-728 (2004). 114. K Arvind Babu, K Lakshmi Rao, M K Kanakavalli, W Surayanarayana, Mamata Deenadayal, Lalji Singh. CYP1A1, GSTM1 and GSTT1 genetic polymorphism is associated with susceptibility to polycystic ovaries in South Indian women. Reproductive BioMedicine, Vol.9. No.2, 194-200 (2004). 115. Ramesh K Aggarwal, D. Udaykumar, P.S. Hendre, A. Sarkar and Lalji Singh. Isolation and characterization of six novel microsatellite markers for mulberry (Morus indica). Molecular Ecology Notes, 4, 477-479 (2004). 116. H. Dixit, M. Deendayal and L. Singh. Mutational analysis of mature peptide region of inhibin genes in Indian women with ovarian failure. Human Reproduction, Vol.19(8), 1760-1764 (2004). 117. Sunil Kumar Verma, Ravindra Kumar Sinha and Lalji Singh. Phylogenetic position of Platanista gangetica: insights from the mitochondrial cytochrome b and nuclear interphotoreceptor retinoid-binding protein gene sequences. Molecular Phylogenetics and Evolution, Vol 33, 280-288 (2004). 118. Langstiesh BT, Reddy BM, Thangaraj K, Singh L. Genetic Diversity and Relationships among the tribes of Meghalaya compared to other Indian and Continental Populations. Hum Biol. 76:569-590 (2004). 119. Venkata Suryanarayana, Mamata Deenadayal and Lalji Singh. Association of CYO1A1 gene polymorphism with recurrent pregnancy loss in the South Indian population. Human Reproduction, Vol. 19, No.11 2648-2652 (2004). 120. K. Shanker, J. Ramadevi, B.C. Choudhury, L. Singh, R.K. Aggarwal. Phylogeography of olive ridley turtles (Lepidochelys olivacea) on the east coast of India: implications of conservation theory. Molecular Ecology 13(7) 1899-1909 (2004). 121. Lakshmi Rao, Arvind Babu, Venkata Padmalatha, Murthy Kanakavalli, Mamata Deenadayal and Lalji Singh. Novel X-chromosomal defect associated with abnormal ovarian function. J. Obstet. Gynaecol. Res. Vol. 31 (1), 12-15 (2005). 122. Kumarasamy Thangraj, Vempati Sridhar, Toomas Kivisild, Alla G. Reddy, Gyaneshwer Chaubey, Vijay Kumar Singh, Suminder Kaur, Pooja Agarwal, Amit Rai, Jalaj Gupta, Chandana Basu Mallick, Niraj Kumar, Thrimulaisamy P Velavan, Rajanbabu Suganthan, Divi Udaykumar, Rashmi Kumar, Rachana Mishra, Arif Khan, Chitikineni Annapurna, Lalji Singh. Different population histories of the Mundari- and Mon-Khmer-speaking Austro-Asiatic tribes inferred from the mtDNA 9-bp deletion/insertion polymorphism in Indian populations. Hum. Genet. Vol. 116, 507-517 (2005). 123. Reddy BM., Naidu VM, Madhavi VK, Thangaraj K, Langtieh BT, Venkataramana P, Kumar V, Singh L. STR Data for the Amp FlSTR Profiler Plus Loci Among 27 Populations of Different Social Hierarchy from Southern Part of Andhra Pradesh, India. Forensic Sci. Intl. 149: 81-97 (2005). 124. K Thangaraj, Gyaneshwar, Thomas Kivisile, A.G. Reddy, Vijay Singh, Avinash Rasalkar and Lalji Singh. Reconstructing the origin of Andaman Islanders. Science Vol. 308; 996 (2005). 125. M. Sachdev, R. Sankaranarayanan, P. Reddanna, K. Thangaraj, L. Singh. Major histocompatibility complex class I polymorphism in Asiatic lions. Tissue Antigens Vol. 66: 9-18 (2005). 126. K Lakshmi Rao, K Arvind Babu, M K Kanakavalli, V V Padmalatha, Mamata Deenadayal and Lalji Singh. Prevalence of chromosome defects in azoospermic and oligoasthenoteratozoospermic South Indian infertile men attending infertility clinic, Reproductive Biomedicine Online Vol. 10, No. 4. 467–472 (2005). 127. Sandeep Kumar Gupta, Sunil Kumar Verma and Lalji Singh. Molecular insight into a wildlife crime: the case of peafowl slaughter, Forensic Science International Vol. 154 No.2-3. 214-217 (2005). 128. K Anil Kumar, K Lakshmi Rao, Suryanarayana V Vedula, Kanakavalli M K, Padmalatha V Vaddamani, Mamta Deendayal and Lalji Singh. Screening of Galactose-1-Phosphate Uridyltransferase gene in Indian women with ovarian failure, Reproductive Bio Medicine Online Vol.11.No 4. 44-448 (2005). 129. Hridesh Dixit, Lakshmi K. Rao, Venkata Padmalatha, Murthy Kanakavalli, Mamatha Deenadayal, Nalini Gupta, Baidyanath N. Chakravarty, Lalji Singh (2005). Mutational screening of coding region of Growth Differentiation Factor 9 gene in Indian women with ovarian failure, Menopause Vol 12 No.6. 749-754 (2005). 130. K Lakshmi Rao, M K Kanakavalli, K Arvind Babu, V V Padmalatha, Mamata Deenadayal and Lalji Singh. Chromosome inversions and a novel chromosome insertion associated with recurrent miscarriages in South India, Archives of Obstetrics and Gynecology 272(4) 273-7 (2005). 131. Lalji Singh, Thangaraj, G R Chandak, et al from CCMB: The Indian Genome Variation Consortium. Human Genet (2005) Vol. 1-11 132. Rajender Singh, Selvi R. Deepa, Sakhamuri Madhavi, Nalini J. Gupta, Baidyanath Chakravarty, Lalji Singh, Kumarsamy Thangaraj. Male infertility: No Evidence of Involvement of Androgen Receptor Gene among Indian Men. J. Androl. 27(1) 102-105 (2006). 133. Kumarasamy Thangaraj, Gyaneshwer Chaubey, Toomas Kivisild, Alla G Reddy, Vijay Kumar Singh, Avinash A Rasalkar and Lalji Singh: Response to Comment on “Reconstructing the Origin of Andaman Islanders”. Science: Vol. 311. 470b (2006). 134. Singh Rajender, Vutukuri Rajani, Nalini J. Gupta, Baidyanath Chakravarty, Lalji Singh, Kumarasamy Thangaraj: SRY negative 46,XX male with normal genitals, complete masculinization and infertility. Mol Hum Reprod. doi:10.1093/molehr/gal030). 135. Thangaraj K, Deepa SelviRani, Pavani K, Gupta NJ, Reddy P, Reddy AG, Chakravarty BN, Singh L (2006) A to G transitions at 260, 386 and 437 in DAZL gene are not associated with spermatogenic failure in Indian population. Intl. J. Androl. (doi:10.1111/j.1365-2605.2006.00685.x) 136. Thangaraj K, Chaubey G, Singh VK, Reddy AG, Pavate PP, Singh L Genetic Profile of Nine Autosomal STR Loci Among Halakki and Kunabhi Populations of Karnataka, India. J. Forensic Sci. 51: 190-192 (2006). 137. Vikrant Kumar, Banrida T. Lasngstieh, Komal V Madhavi, Vegi M. Naidu, Hardeep Pal Singh, Silpak Biswas, Kumarasamy Thangaraj, Lalji Singh, B. Mohan Reddy Global patterns in Human mtDNA Versus Y-chromosome Variation Caused by Spatial Instability of the Local Cultural Processes. PloS Genetics Vol.2, Issue 4 e53 (2006). 138. Golla N. Prasad Prasad, Ayyasamy Vanniarajan, Cyril Emmanuel, Kotturathu Mammen Cherian, Lalji Singh, Kumarasamy Thangaraj K. Novel mitochondrial DNA mutations in a rare variety of hypertrophic cardiomyopathy. Intl. J. Cardiol. 109: 432-433 (2006). 139. Vanniarajan A, Nayak D, Reddy AG, Singh L, Thangaraj K. Clinical and genetic uniqueness in an individual with MELAS. Am. J. Med. Genet 141B: 440-444 (2006) 140. Kumarasamy Thangaraj, Gyaneshwer Chaubey, Vijay Kumar Singh, Ayyasamy Vanniarajan, Ismail Thanseem, Alla G. Reddy, Lalji Singh In situ origin of deep rooting lineages of mitochondrial Macrohaplogroup M in India BMC Genomics 7:151 (2006) 141. Vanniarajan A, Rajshekher GP, Joshi MB, Reddy AG, Singh L, Thangaraj K (Novel mitochondrial mutation in the ND4 gene associated with Leigh Syndrome. Acta Neurol Scand DOI: 10.1111/j.1600-0404.2006.00673.x (2006). 142. Venkata Suryanarayana, Lakshmi Rao, Murthy Kanakavalli, Venkata Padmalatha, Mamata Deenadayal, Lalji Singh. Recurrent early pregnancy loss and endothelial nitric oxide synthase gene polymorphisms. Arc. Gynecol. Obstet. 274, 119-124 (2006). 143. Rajender Singh, Prabhakar K Shastry, Avinash A Rasalkar, Lalji Singh and K Thangaraj. A novel androgen receptor mutation resulting in complete androgen insensitivity syndrome and bilateral Leydig cell hyperplasia. Journal of Andrology (2006) 144. Gupta SK, Thangaraj K, Singh L. A Simple and Inexpensive Molecular Method for Sexing and Identification of the Forensic Samples of Elephant Origin. J. Forensic Sci. 51 (2006). http://www.blackwell-synergy.com/doi/abs/10.1111/j.1556-4029.2006.00154.x 145. Singh Rajender, Kumarasamy Thangaraj, Nalini J. Gupta, N. Leelavathy, Deepa Selvi Rani, Renjini G Nambiar, Vadivelu Kalavathy, Sathiyavedu T Santhiya, Sayee Rajangam, Puthiya M. Gopinath, Baidyanath Chakravarty, Lalji Singh. A Novel human sex-determining gene linked to Xp11.21-11.23. J. Clin. Endocrin. Metab. (2006) 4028-4036. 146. Kumar V, Langsiteh BT, Biswas S, Babu JP, Rao TN, Thangaraj K, Reddy AG, Singh L, Reddy BM. Asian and Non-Asian Origins of Mon-Khmer and Mundari Speaking Austro-Asiatic populations of India. Am. J. Hum. Biol. 18: 461-469 (2006) 147. Thanseem I, Thangaraj K, Chaubey G, Singh VK, Bhaskar LVKS, Reddy BM, Reddy AG, Singh L. Genetic Affinities Among the Lower Castes and Tribal Groups of India: Inference from Y Chromosome and Mitochondrial DNA. BMC Genetics 7:42 (2006). 148 Hridesh Dixit, Lakshmi Rao, Venkata V Padmalatha, Murthy Kanakavalli, Mamata Deenadayal, Nalini Gupta, Baidyanath Chakrabarty, Lalji Singh. Missensemutations in the BMP15 gene are associated with overrian failure.Human Gennet (2006) 119: 408-417 148. H.Dixit, Lakshmi Rao, V V Padmalatha, Murthy Kanakavalli, Mamata Deenadayal, Nalini Gupta, B.N. Chakrabarty, Lalji Singh Missence Mutations in the BMP15 gene ara associated with ovarian, Human Gennet (2006 ) 119: 408 - 415 149. H.Dixit, Lakshmi Rao, V V Padmalatha, Murthy Kanakavalli, Mamata Deenadayal, Nalini Gupta, B.N. Chakrabarty, Lalji Singh. Mutational analysis of the betaglycan gene-coding region in susceptibility for overian failure Human Reproduction (2006) 1-6 150. H. Dixit, M. Deendayal and Lalji singh. Mutational analysis of the mature peptide region of inhibin genes in Indian women with ovarian failure Human Reproduction Vol.19,No.8 pp.1760-1764,(2004) 151. Ramana Gutala, Denise R. Carvalho-Silva, Li Jin, Bryndis Yngvadottir, Vasanthi Avadhanula, Khaja Nanne, Lalji Singh, Ranajit Chakraborty and Chris Tyler-Smith: A shared Y-chromosomal heritage between Muslims and Hindus in India. Hum Genet (2006) DOI 10.1007/s00493-006-0234-x 152. Nikita Thakur, Nageshwar D Reddy, Venkateshwar G Rao, Mohan P Krishna,,Lalji Singh and Giriraj R Chandak : A novel mutation in STK11 gene is associated with Peutz-Jeghers Syndrome in Indian patients. BMC Medical Genetics (2006) doi:10.1186/1471/-2350-7-73. 153. Tatiana Zerjal, Arpita Pandya, Kumarasamy Thangaraj, Edmund Y. S. Ling, Jennifer Kearley, Stefania Bertoneri, Silvia Paracchini, Lalji Singh, Chris Tyler-Smith: Y-chromosomal insights into the genetic impact of the caste system in India" Human Genet (2007) 121:137-144 (DOI 10.1007/s00439-006-0282-2). 154. Swapna Mahurkar, Mohammed M Idris, Nageshwar D Reddy, Seema Bhaskar, Venkateshwar G Rao, Varghese Thomas, Lalji Singh and Giriraj Ratan Chandak: Association of cathepsin B gene polymorphisms with tropical Calcific pancreatitis. Gut (2006) doi:10.1136/gut.2005.087403. 155. Deepa-SelviRani, Vanniarajan A, Gupta NJ, Chakravarty BN, Singh L, Thangaraj K: A novel missense mutation C11994T in the mitochondrial ND4 gene as a cause of low sperm motility in the Indian subcontinent. Fertility and Sterility (2006) doi:10.1016/j.fertnstert.2006.04.044. 156. Bhagavatula J, Singh L. Genotyping faecal samples of Bengal tiger Panthera tigris tigris for population estimation: a pilot study. BMC Genet. 2006 Oct 17;7:48. http://www.biomedcentral.com/1471-2156/7/48 157. Ajay Gaur, Kesaraju Shailaja, Anju Singh, Veluri Arunabala, Borusu Satyarebala & Lalji Singh: Twenty polymorphic microsatellite markers in the Asiatic lion (Panthera leo persica). Conservation Genetics (2006) 7:1005-1008. DOI 10.1007/s10592-006-9121-1. 158. Singh Rajender, Lalji Singh, Kumarasamy Thangaraj: Phenotypic heterogeneity of mutations in androgen receptor gene. Asian J Androl (2007); 9:147-149. 159. Zeenath Jehan, Sambandam Vallinayagam, Shrish Tiwari, Suman Pradhan, Lalji Singh, Amritha Suresh, Hemakumar M. Reddy, Y.R. Ahuja and A. Jesudasan: Novel noncoding RNA from human Y distal heterochromatic block (Yq12) generates testis-specific chimeric CDC21_2. Genome Research (2007), 17: 433-440. 160. Seema Bhaskar, DN Reddy, Swapna Mahurkar, GV Rao, Lalji Singh and Giriraj Chandak: Lack of significant association of an insertion/deletion polymorphism in the angiotensin converting enzyme (ACE) gene with tropical calcific pancreatitis. BMC Gastroenterology published online December 12, 2006. doi:10.1186/1471-230X-6-42. 161. Mala Ganesan, Khanderao R. Paithankar, Medicharla V. Jagannadham, Curam S. Sundaram, Bulusu S. Murthy, Lalji Singh: Characterization of novel DNA-binding proteins expressed in snake oocyte cDNA library. J. Protein Expression and Purification 53 (2007)164-178; doi: 10.1016/j.pep.2006.11.007. 162. R K Aggarwal, T Kivisild, J Ramadevi and L Singh: Mitochondrial DNA coding region sequences support the phylogenetic distinction of two Indian wolf species. J Zool Syst Evol Res (2007) 45(2), 163-172; doi: 10.1111/j.1439-0469.2006.00400.x. 163. Vikrant Kumar, Arimanda N S Reddy, Jagadish P Babu, Tipirisetti N Rao, Banrida T Langstieh, Kumarasamy Thangaraj, Alla G Reddy, Lalji Singh and Battini M Reddy: Y-chromosome evidence suggests a common paternal heritage of Austro-Asiatic populations. BMC Evolutionary Biology (2007) 7:47; doi:10.1186/1471-2148-7-47 164. A. Khattri, R.K. Pandey, N.J. Gupta, B. Chakravarty, M. Deendayal, L. Singh and K. Thangaraj: CA repeat and Rsal polymorphisms in ER gene are not associated with infertility in Indian men. International Journal of Andrology, 30, 1-7 (2007). 165. V. R. Rao, L.V.K.S.Bhaskar, C Annapurna, A.G.Reddy, K Thangaraj, A.Papa Rao, Lalji Singh: Single Nuceotide Polymorphisms in Alcohol Dyhydrogenase Genes Among Some Indian Population. American Journal of Human Biology 338-344 (2007) 166. K. Thangaraj, G. Chaubey, A.G. Reddy, V.K. Singh, L. Singh. Autosomal STR data on the enigmatic Andaman Islanders. Forensic Sci. Intl. 169, 247-251 (2007). 167. Venkata V Suryanarayana, Lakshmi Rao, Murthy K Kanakavalli, Venkata V Padmalatha, Mamata Deenadayal, Lalji Singh. Role of CYP17 and CYP19 polymorphisms in idiopathic recurrent miscarriages among South Indian Women. Reproductive BioMedicine Online. 14, 341-347 (2007) 168. Thangaraj K, Chaubey G, Singh VK, Reddy AG, Chauhan P, Malvee R, Pavate PP, Singh L:.Y-Chromosomal STR Haplotypes in Two Endogamous Tribal Populations of Karnataka, India. J Forensic Sci. 52(3):751-3 (2007).
178. Kumarasamy Thangaraj, Gyaneshwer Chaubey, Toomas Kivisild, Deepa Selvi Rani, Vijay Kumar Singh, Thanseem Ismail, Denise Carvalho-Silva, Mait Metspalu, LVKS Bhaskar, Alla G. Reddy, Sharat Chandra, Veena Pande, B. Prathap-Naidu, Niharika Adarsh, Abhilasha Verma, Inaganti Amara Jyothi, Chandana Basu Mallick, Nidhi Shrivastava, Ragala Devasena, Babita Kumari, Amit Kumar Singh, Shailendra Kumar Dhar Diwedi, Shefali Singh, Geeta Rao, Pranav Gupta, Vartika Sonvane, Kavita Kumar, Afsar Basha, K. R Bhargavi, Albert Lalremruata, Aravind Kumar Gupta, Gurukamal Kaur, K. K. Reddy. A. Papa Rao, Richard Villems, Chris Tyler-Smith, Lalji Singh: Maternal Footprints of Southeast Asians in North India. Human Heredity 66(1):1-9 (2007).DOI: 10.1159/000114160. 179. Pramod K Rout, Manjunath B Joshi, Ajoy Mandal, D Laloe, Lalji Singh and Kumarasamy Thangaraj: Microsatellite-based phylogeny of Indian domestic goats. BMC Genetics 9:11 (2008) doi: 10.1186/1471-2156-9-11. 180. Rajender S, Pandu G, Annapurna Ch, Sharma JD, Gandhi KPC, Sing L, Thangaraj K: Reduced CAG repeats length in androgen receptor gene is associated with violent criminal behavior. Intl. J. Legal Med (Published online: 26 March 2008).
181. Bhaskar LVKS, Thangaraj K, Mulligan CJ, Rao AP, Pardhasaradhi G, Kumar KP, Shah AM, Sabeera B, Reddy AG, Singh L, Rao VR (2008) Allelic variation and haplotype structure of the Dopamine receptor gene DRD2 in 9 Indian populations. Genet. Testing 12:153-160 Research Papers (In press) 1. Rani DS, Carlus JS, Poongothai J, Jyothi A, Pavani K , Gupta NJ, Reddy AG, Rajan MM, Chakravarty BN, Singh L, Thangaraj K (2008) CAG repeat variation in the mtDNA polymerase (POLG) is not associated with oligoasthenozoospermia. Intl. J. Androl. (in press). 2. Suryavathi V, Khattri A, Gopal K, Rani DS, Panneerdoss S, Gupta NJ, Chakravarty BN, Deendayal M, Singh L, Thangaraj K (2008) Novel variants in UBE2B gene and idiopathic male infertility. J. Androl. (in press) Research Report 1. Shanker Kartik, B. C. Choudhary, R. K.Aggarwal, Lalji Singh (2000) Conservation genetics of the Olive Ridley sea turtle (Lepidochelys olivacea) on the east coast of India- a report. Wildlife Institute of India, Dehradun, India. Research Papers in Conference Proceedings 1. Aggarwal, Ramesh K., R. Rajkumar, P. Rajendrakumar, Prasad S. Hendre, A. Baruah, R. Phanindranath, V. Annapurna, N. S. Prakash, A. Santaram, C. S. Srinivasan, Lalji Singh (2004). Fingerprinting of Indian Coffee Selections and Development of Reference DNA Polymorphism Panels for creating Molecular IDs for Variety Identification. In: Proceedings of ASIC 2004: 20th International Conference on Coffee Sciences, Banglore, India, 11 –15 October 2004. 2. Aggarwal, Ramesh K., A. Baruah, V. Naik, Prasad S. Hendre, A. Ashraf, P. Rajendrakumar, R. Rajkumar, V. Annapurna, R. Phanindranath, N. S. Prakash, C. S. Srinivasan, Lalji Singh (2004). Development and Characterization of Coffee Specific Microsatellite Markers for use as Potential Genetic Markers. In: Proceedings of ASIC 2004: 20th International Conference on Coffee Sciences, Banglore, India, 11–15 October 2004. 3. Bhat, Prasanna R., V. Krishnakumar, P. S. Hendre, P. Rajendrakumar, Lalji Singh, Ramesh K. Aggarwal (2004). Identification of Putative Resistance Gene Analogues in Coffea and related Psilanthus taxa. In: Proceedings of ASIC 2004: 20th International Conference on Coffee Sciences, Banglore, India, 11 –15 October 2004. E. LIST OF PATENTS AND POPULAR ARTICLES Patents: 1. Verma, S.K., and Singh L,: Universal primers to establish the identity of animal parts and products 2003/7489 dated 24.11.2004 (South Africa – Granted) The US patent was granted on November 28, 2006. Its number is 7,141,364 2. Indian patent on Bkm and Bkm-derived probes has been filed (Application No. 1000 DEL 88), CSIR, New Delhi. Popular Articles:
3. “DNA Testing, Evidence Act and Expert Witness” published in The Indian Police Journal – December 2000 4. Science for Children: an Interview at the Indian Science Congress: 88th Session. In: Food Nutrition and Environmental Security: The Road Ahead. A publication brought out by the National Institute of Science Communication, New Delhi pp.371-375, 2001.
6. Human Genome and Beyond– published in CSIR News Vol. 51 No. 8 April 2001. 7. An article on “The story of a village where birth of identical twins is common” published in EENADU Telugu daily, July 31, 2003. 8. Several articles in Hindi in “Jigyasa’ a periodical brought out by CCMB. 9. Book titled “You Deserve, We Conserve: A Biotechnological Approach to Wildlife Conservation” (by Dr Lalji Singh and Dr M W Pandit) unveiled by H.E. Dr A P J Abdul Kalam, Hon’ble President of Indian on the occasion of dedication of LaCONES to the nation on 1st February 2007. 10. Book Titled “Scientoonic Tell-Tale of Genome and DNA” (by Dr Lalji Singh, Dr M W Pandit and Dr Pradeep Srivastava) F. INFORMATION ON AND RELEVANCE OF RESEARCH CONTRIBUTIONS 1. Development of a universal probe for DNA fingerprinting Dr Singh and his colleagues in the CCMB, at Hyderabad, developed a probe called Bkm-derived probe for DNA fingerprinting, as a fall out of their earlier internationally well-known work on the mechanisms of determination of sex. This indigenously developed probe is being extensively in use for forensic investigations, paternity determinations and seed stock verifications. DNA fingerprinting evidence was presented in the court and for the first time in the annals of Indian history this result was accepted as an infallible evidence in the court of law. This verdict was upheld by the Kerala High Court. Since then, this technology was used in 500 cases of paternity disputes, identification of missing children, identification of mutilated bodies, identification of exchanged babies in hospitals and rape and murder cases, etc. These include sensational cases of assassination of the late Prime Minister Shri Rajiv Gandhi, assassination of Punjab Chief Minister, Swami Premananda case and the famous tandoor case of Naina Sahni. Setting up of CDFD A separate autonomous "Centre for DNA Fingerprinting and Diagnostics" (CDFD) has been set up by the Department of Biotechnology, Government of India to apply and further develop this technology for the benefit of the country. Dr Lalji Singh, as the First In-Charge Officer on Special Duty to this Centre, had immensely contributed to its present status (From Oct.1995 to Feb.1999) in addition to his research and other responsibilities in the CCMB. The Centre is already providing DNA diagnostic services for many genetic disorders such as Thalassemia, Sickle Cell Anemia, Mental Retardation, Fragile-X Syndrome, Duchenne Muscular Dystrophy, Huntington's Disease, Azoospermia, etc. The ultimate aim of the Centre is to develop, acquire and standardize the protocols for carrier detection, prenatal diagnosis and genetic counseling for all the genetic disorders prevalent in our country. This is an important achievement for CCMB and CSIR. In recognition of this contribution, Dr Singh and his group have recently been awarded the CSIR Technology Award. 2. DNA fingerprinting using indigenously developed Bkm-derived probe for phylogenetic analyses DNA Fingerprinting has so far been used only in individual identification and establishment of biological relationships owing to its extreme power of resolving individual specific variation present in the genome. At the CCMB, Dr Singh and his colleagues by using the indigenously developed Bkm-derived probe, which is successfully used for human DNA fingerprinting, have shown, for the first time, that DNA fingerprinting can effectively be used to infer the generic affinities among related group of animals like crocodilians. This was hitherto thought not to be feasible largely because the fingerprint profiles are believed to evolve too rapidly to be informative over large time intervals. Based on qualitative differences in the fingerprints and quantitative differences in the copy number of Bkm-related sequences in the genomes, they have been able to infer generic affinities among different species/genera of crocodilians, which are in agreement with the consensus phylogeny reconstructed using various other approaches together. This observation is of great importance as it establishes, for the first time, the potential utility of this molecular technique in the study of evolutionary relationships of plants and animals. Dr Lalji Singh is presently using this for wild life preservation and better management of endangered species in our zoos and also for identification and isolation of genes for useful characters in silkworm races. This technology is also being used for medical diagnosis. 3. Studies on molecular basis of sex determination Isolation of highly conserved sex chromosome-specific satellite DNA, 'Bkm', from the female Indian snake, the Banded Krait, made the beginning of the understanding of the molecular basis of sex-determination, which is one of the most important unsolved problems in modern biology. Now, this has also become the basis of our understanding of sex-reversal in humans. For the first time, Dr Lalji Singh and his colleagues have reported a unique case of sex reversal of an individual who has the 47,XXY chromosome constitution but a female phenotype. This finding of the occurrence of XXY female with normal SRY, ZFY and SOX9 genes testifies the involvement of other gene(s) in sex determination. 4. Sex and germ cell-specific Bkm-binding protein Bkm sequences in snakes, Bkm-associated Y-specific sequences p102d(2) in human and M34 in mouse, are interspersed amongst other sequences along the entire length of the sex-determining chromosomes. The consensus sequence in these sex chromosome specific repeats is the conserved tetranucleotide repeat GATA component of Bkm. Dr Singh and his associates have purified a sex- and germ-cell-specific protein from snake ovary, which specifically binds GATA repeats of Bkm. This may be the potential signal responsible for the decondensation (activation) of the sex-determining chromosome. In silkworm Bombyx mori BKM-binding protein (BBP) is expressed predominantly in pupal ovary suggesting its probable role in bringing about coordinated conformational changes in chromatin to activate genes present in associated chromosomal domains. 5. Wildlife Conservation- Genetic variation in Asiatic lions and Indian tigers Previous reports suggested that Asiatic lions and tigers in India are highly inbred and exhibit very low levels of genetic variation. Dr Singh and his colleagues' analyses on these species have shown much higher degree of polymorphism than reported. Analysis of 38 Asiatic lions, which exist as a single population in the Gir Forest Sanctuary in India, revealed an average heterozygosity of 25.82%. In Indian tigers, microsatellite analysis and multilocus fingerprinting on a population of 22 individuals revealed a heterozygosity of 22.65%. Microsatellite analysis has enabled the identification of the pure Asiatic lions from the hybrids. Similar analysis of hair samples enabled them to identify hybrids of Indian and Siberian tigers. Microsatellite analysis was performed on 50-125 years old skin samples from museum specimens. Their results show similar levels of genetic variability as in the present population (21.01%). Studies of Asiatic lions and tigers have further helped in identifying individuals with high genetic variability which can be used for conservation breeding programmes. Recently Dr Singh’s group isolated highly polymorphic microsatellite loci from a partial genomic library of the Asiatic lion, which show much higher levels of variation. Setting up of LaCONES Based on the studies of Dr Lalji Singh and his colleagues on Wildlife Conservation, Government of India has taken a decision to set up a “Laboratory for the Conservation of Endangered Species (LaCONES)” in Hyderabad close to the Nehru Zoological Park in collaboration with the Central Zoo Authority of India, New Delhi; the Dept. of Forests, Govt. of A.P, Hyderabad; the Centre for Cellular & Molecular Biology (CCMB), Hyderabad and the Dept. of Biotechnology (DBT), New Delhi. The civil construction of the facility is now complete to the extent of 80-90%. The scientists have already developed a semen cryobank of tigers, lions, and leopards and hope to create egg, embryo and cell banks for future use. 6. A novel universal approach of species identification for forensic applications A PCR-based approach has been established which, without knowing the history of a forensic sample, can reveal whether the source of the sample is human or animal, and if animal, which of the 221 animal species. Primers used in these studies are universal, and thus can be used for number of other species. This approach could be an ultimate solution for the identification of species for forensic applications. 7. Conservation genetics of Eld’s deer Eld’s deer is a highly endangered cervid distributed historically throughout much of South Asia and Indo-China. Mitochondrial DNA control region was analyzed in these species to get better understanding of genetic population structure and evolutionary history. The results suggest a strong degree of phylogeographic structure, both between subspecies and among population within subspecies, indicating that dispersal of individuals between populations has been very limited historically. These findings will be useful for continued managements of these species. 8. Genetic affinities of Andaman Islanders Dr Lalji Singh and his group have undertaken the study on genetic diversity in primitive tribes of India including the tribal populations of Andaman and Nicobar islands using Y-chromosomal markers and mtDNA sequences. Data indicates that the Andamanese have closer affinities to Asian than to African populations, and suggests that they are the descendants of the early Paleolithic colonizers of Southeast Asia – the hunter gatherers and the first migrants moved out of Africa about 60,000-100,000 years ago. 9. DNA based molecular diagnostics During the studies on various patients from Andhra Pradesh, Dr Lalji Singh and his group came across several interesting cases which many a times become the basis for further clinical research in terms of their genetic association and allelic variation. Sickle cell anemia, Alzheimer’s disease, BCD, male infertility, pancreatitis, and Robert’s syndrome are some such examples. These studies have further helped in better understanding of the role played by genetic factors in these diseases, and the possibility of developing better diagnostic tools for the detection and the management of such diseases. 10. A highly conserved novel human gene implicated in testicular development Dr Lalji Singh and his group identified and characterized a novel human gene, which is predominantly expressed in testis showing two special variants. The over-expressed GFP fusion protein localizes to the nucleus, suggesting a regulatory function to the protein. The knock out of the gene resulted in sterility in male mice with abnormal testis completely devoid of germ cells; this testifies involvement of this gene in testicular development. Yüklə 332,01 Kb. Dostları ilə paylaş:
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