Dr. Md. Talib Hussain is an Assistant Professor of Pharmacology at College of Pharmacy, University of Haill, KSA. He holds more than nine years (including 2 Yrs. Post Ph.D. experience) of research and teaching experience. He is an associate member of Quality and development unit in the College of Pharmacy, UOH.
Research Keywords: Evaluation of natural and synthetic compounds for acute (LD50) determination and subacute toxicity, for their specific pharmacological in-vivo studies like hepatocellular carcinoma (HCC), anti-hepatotoxicity, anti-ulcer, anti-diabetic, diuretic, anti-diarrhoeal, immunomodulatory, anti-inflammatory, analgesic, neurotoxicity, behavior activity and cerebral ischemia in experimental animals as well as in-vitro techniques used to evaluate different anticancer activity by using cell line etc..
Techniques keywords: DNA estimation, Commet assay and Gel electrophoresis, animal handling and dissection of different organs such as liver, kidney, brain, lungs, spleen, heart and stomach etc. , haemopoietic, enzymetic and biochemical estimations (SOD, CAT, GSH, LPO, GPx, LFT etc.), handling of various screening equipment’s used in experimental pharmacology.
Dr. Md. Talib Hussain has extensively published research and review articles in international database. Recently he has authored one book– Phytochemical studies and Antibacterial effects of Coccinia indica. (2012). LAMBERT Academic Publishers, Germany. ISBN 978-3-8484-8711-0, pp 1-116
He has taught both Post graduate course and undergraduate courses. He has supervised 12 Post graduate thesis research project at the Integral University, India. His favorite part of teaching is helping a struggling student to achieve their goals.
Apart from theory classes he use to take practical classes also which include Computer Simulated experiments andanimal model such as:
Evaluation of drugs on isolated tissues viz. frog heart, rat ileum, stomach strip, colon..
Matriculation- Bihar Secondary Examination Board, Patna, India 65.40 %
Research techniques summary
Worked as Project fellow in ICMR sponsored project from May 2006 - Oct 2006 at Ethnopharmacology Division, National Botanical Research Institute (CSIR), Lucknow, India.
During this period I carried out the following research activities-
Collection and systematic extraction of the plant materials with solvent of different polarity followed by fractionation.
Quality control parameter investigated as Preliminary phytochemical screening of the different fraction plant for the identification of various active constituents viz. alkaloid, carbohydrate, flavonoid, amino acid, saponin, phytosterol, phenolic compound, tannin, gum and resin, etc.,
HPTLC analysis was performed.
Toxicological studies for the actual LD50 determination was done by using mice of both sexes.
Induction of hepatocellular carcinoma (HCC) in rats were given a single intraperitoneal injection of N-nitrosodiethylamine (NDEA) (200 mg/kg body weight) followed by subcutaneous injections of carbon tetrachloride (CCl4) (3 ml/kg body weight/week) for 6 weeks.
Carried out detailed chemical biomarker investigations like Serum was analyzed for the serum glutamic oxaloacetic transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP) bilirubin (BL) and gamma glutamate transpeptidase (GGT).
Liver was also analyzed for superoxide dismutase (SOD), catalase (CAT), lipid peroxide (LPO), glutathione peroxidase(GPx), and glutathione-S-transferase (GST) showing in treatment of hepato cellular carcinoma.
Statistical analysis was carried out by using one way ANOVA.
Area of expertise Evaluation of natural (phytoconstituent)/synthetic compounds/formulation for acute (LD50) and subacute toxicity, for their specific pharmacological in-vivo studies like hepatocellular carcinoma (HCC), anti-hepatotoxicity, anti-ulcer, anti-diabetic, diuretic, anti-diarrhoeal, immunomodulatory, anti-inflammatory, analgesic, neurotoxicity and behavior activity as well as in-vitro techniques used to evaluate different anticancer activity by using cell line etc.
Design and development of different biological screening model in rodents.
Different molecular Pharmacological techniques like DNA estimation, Commet assay and Gel electrophoresis.
Evaluation of drugs on isolated tissues viz. frog heart, rat ileum, stomach strip, colon.
Evaluation of 3/4 point bioassay of different drugs.
Development/investigation of liver/lung cancer in rats.
Animal handling and dissection of different organs such as liver, kidney, brain, lungs, spleen, heart and stomach etc.
Collection of blood by retro orbital, tail vein as well as heart puncture.
Administration of drugs by oral, i.p, i.m, i.v. and s.c. route.
Haemopoietic, enzymetic and biochemical estimations.
Participated in INSA anniversary meet 2013, “mentoring session for Final Year PhD students by INSA Young Scientist Awardees” held on 28th of December.
Participated in national seminar on recent trends in drug development Held on 9th January 2014, organized by Amity University, Lucknow campus.
Certificate of achievement is awarded for the participation and successful completion of the 1st national level workshop on “Intellectual property system:India today and tomorrow” organized by intellectual property rights management cell (IPRMC),CCG&D, Integral University ,lucknow in collaboration intellectual property owners association(IPOA),New Delhi, held on 10th January 2014.
Research Paper Published (as per impact factor)
Abdul Qadir, Md Faiyazuddin, Md Talib Hussain, Sarfaraz Ahmad, Thamer M. Alshammari, Faiyaz Shakeel,"Critical steps and energetics involved in a successful development of a stable nanoemulsion",Journal of Molecular Liquids214 (2016), 7-18. (IF-2.52)
Usama Ahmad, Md Faiyazuddin, Md Talib Hussain, Sarfaraz Ahmad, Thamir M Alshammari, Faiyaz Shakeel. Silymarin: an insight to its formulation and analytical prospects. Acta Physiologiae Plantarum November 2015, 37:253. (IF-1.58)
Talib Hussain, Hefazat H. Siddiqui, Sheeba Fareed, Vijayakumar M., Chandana V. Rao. Chemopreventive evaluation of Tephrosia purpurea against NDEA induced hepatocarcinogenesis in Wistar rats. Journal of Pharmacy and Pharmacology 2012; 64: 1195–1205.(IF-2.17)
Talib Hussain, Hefazat H. Siddiqui, Sheeba Fareed, Vijayakumar M., Chandana V. Rao. Evaluation of chemopreventive effect of Fumaria indica against N-nitrosodiethylamine and CCl4-induced hepatocellular carcinoma in Wistar rats. Asian Pacific Journal Tropical Medicine 2012; 5: 623-629. (IF-1.06).
Talib Hussain, Ramesh K Gupta, K Sweety, Bavani Eswaran, M Vijayakumar, Chandana Venkateswara Rao. Nephroprotective activity of Solanum xanthocarpum fruit extract against gentamicin–induced nephrotoxicity and renal dysfunction in experimental rodents. Asian Journal of Tropical Medicine 2012; 5: 686-691. (IF-1.06)
Ramesh K Gupta, Talib Hussain, G Panigrahi, Avik Das, Gireesh Narayan Singh, K Sweety, Md Faiyazuddin, Chandana Venkateswara Rao. Hepatoprotective effect of Solanum xanthocarpum fruit extract against CCl4 induced acute liver toxicity in experimental animals. Asian Pacific Journal Tropical Medicine 2011; 4: 964-968. (IF-1.06)
Shrestha Bajpai, Ravi Pathak, Talib Hussain. Anti-Inflammatory Activity of Ethanobotnical Plants Used as Traditional Medicine: A Review. Research and Reviews: Journal of Botanical Sciences 2014; 3: 8-18.
Ramesh Kr. Guptaa, Rajnish Kr. Singh, Talib Hussain, Jagannath Sahoo, Ashok Kumar Gupta, Sudhansu Ranjan Swain, Umakant Bajaj, Chandana Venkateswara Rao. Antihepatotoxic and antioxidant potential of Hedyotis corymbosa extract against antitubercular drugs induced hepatotoxicity in experimental animal. The Journal of Toxicology and Health 2013; 103: 162-170.
Muhammad Arif, Sheeba Fareed, Talib Hussain, Mohammad Ali. Adaptogenic activity of lanostane triterpenoid isolated from Carissa carandas fruit against physically and chemically challenged experimental mice. Pharmacognosy Journal 2013;5: 216-220.
Talib Hussain, Hefazat H. Siddiqui, Sheeba Fareed, Vijayakumar M., Chandana Venkateswara Rao. Acute and subacute oral toxicity evaluation of Tephrosia purpurea extract in rodents. Asian Pacific Journal of Tropical Disease2012; 2: 129-32.
Talib Hussain, Hefazat H Siddiqui, Sheeba Fareed, K Sweety, M Vijayakumar, Chandana V Rao. Chemopreventive effect of Fumaria indica that modulates the oxidant-antioxidant imbalance during N-nitrosodiethylamine and CCl4-induced hepatocarcinogenesis in Wistar rats. Asian Pacific Journal of Tropical Biomedicine 2012; 2: S995-S1001.
Ramesh Kr. Gupta, Rajnish Kr. Singh, Sudhansu Ranjan Swain, Talib Hussain, Chandana Venkateswara Rao. Anti–hepatotoxic potential of Hedyotis corymbosa against D–galactosamine induced hepatopathy in experimental rodents. Asian Pacific Journal of Tropical Biomedicine2012; 2: S1542-S1547.
Talib Hussain, Ramesh K Gupta, Sweety K, Mohd Sajid Khan, Md Sarfaraj Hussain, Md Arif, Arshad Hussain, Md Faiyazuddin, Chandana Venkateswara Rao. Evaluation of antihepatotoxic potential of Solanum xanthocarpum fruit extract against antitubercular drugs induced hepatopathy in experimental rodents. Asian Journal of Traditional Biomedicine 2012; 6: 454-460.
Md. Talib Hussain, Arti R. Verma, M. Vijayakumar, Alok Sharmaand Ch. V. Rao. Rutin, a natural flavonoid, protects against gastric mucosal damage in experimental animals. Asian Journal of Traditional Medicine 2009; 4: 188-197.
Sharma Alok , Vijayakumar M., Verma Arti Raj, Talib Md., Unnikrishnan M. K., Rao Ch.V.In vitro amylase inhibition and antihyperglycemic activity of Helicteres isora in Streptozotocin-Induced rats. International Journal of Pharmaceutical and Clinical Research 2009; 1: 15-18.
Ch V. Rao, Md. Talib Hussain, M.Vijay Kumar, Arti R. Verma, Nishant Kumar, M. Vijayakumar, G.D. Reddy. Evaluation of the analgesic and anti-inflammatory activity of Moringa concanesis tender fruits. Asian Journal of Traditional Medicine 2008; 3; 95-103.