Electronic Posters: Cardiovascular

Wednesday 13:30-15:30 Computer 40

13:30 3749. RGD-Functionalized Superparamagnetic Nanoemulsions for Target-Specific Imaging of Tumor Angiogenesis

Lisette Helene Deddens¹, Peter A. Jarzyna², Arjan W. Griffioen³, Zahi A. Fayad², Rick Michiel Dijkhuizen¹, Willem JM Mulder<sup>2

1</sup>Image Sciences Institute, University Medical Center Utrecht, Utrecht, Netherlands; ²Imaging Science Laboratories, Mount Sinai School of Medicine, New York, United States; ³Angiogenesis Laboratory Amsterdam, VU Medical Center, Amsterdam, Netherlands

Nanoemulsions represent an attractive delivery platform for hydrophobic compounds since they improve their bioavailability and make their intravenous administration possible. This abstract demonstrates that the nanoemulsion platform, developed for passive delivery of hydrophobic compounds to tumor tissue, is also very suitable for targeted applications. Data show the applicability of αvβ3-specific RGD nanoemulsions in targeting tumor angiogenesis visualized by MRI, fluorescence microscopy and immunohistochemistry.

14:00 3750. Angiogenesis and Cell Tracking with Iron Oxide-Labeled Tumor Cells: Correlation Between Cell Growth and the Formation of the Tumor Vascular Bed Using High Resolution Magnetic Resonance (MR) Angiography, T1, T2 and T2* Mapping and Histology

Piotr A. Wielopolski¹, Gyula Kotek¹, Sandra van Tiel¹, Gabriela Doeswijk¹, Lejla Alic², Gabriel P. Krestin¹, Bernsen Monique<sup>1

1</sup>Radiology, Erasmus Medical Center, Rotterdam, zuid-holland, Netherlands; ²Informatics and Radiology, Erasmus Medical Center, Rotterdam, zuid-holland, Netherlands

To correlate super paramagnetic iron oxide (SPIO) labeled tumor cell growth and distribution with high resolution magnetic resonance (MR) angiography, T1, T2 and T2* parametric mapping and histology

14:30 3751. Targeted New Peptide Based Nanoparticles Toward High EGFR Expressing Cancer Cells for MRI

Ming-Hung Chen¹, Gin-Chung Liu^2,3, Twei-Shiun Jaw^2,4, Yu-Ting Kuo^2,3, Chiao-Yun Chen^2,3, Yun-Ming Wang<sup>1

1</sup>Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan; ²Department of Medical Imaging, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; ³Department of Radiology,Faculty of Medicine,College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; ⁴Department of Radiology,Faculty of Medicine,College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

In this study, the various core sizes of manganese ferrite nanoparticles (MnFe2O4) conjugated with D4 peptide (MnFe2O4-PEG-D4) were synthesized. The high relaxivity MnFe2O4 nanoparticles were obtained by thermal decomposition of Iron acetylacetonate and manganese acetylacetonate in hydrophobic solution at high boiling process. The surface of MnFe2O4 nanoparticles were coated with polyethylene glycol (PEG) and EGFR peptide ligand (D4: Leu-Ala-Arg-Leu-Leu-Thr) to improve their dispersion and ability to target EGFR. The negative signal enhancement of EGFR expressing cancer cells (SKBR-3 and PC-3) were significantly higher than that of low EGFR expressing cells (HEK-293).

15:00 3752. In Vivo Detection of a PARACEST Agent in Mouse Brain Tumors

Alex Xuexin Li¹, Mojmir Suchy², Chunhui Li¹, Claire Poppe¹, Joseph Gati¹, Susan Meakin¹, Robert H.E. Hudson², Ravi S. Menon¹, Robert Bartha<sup>1

1</sup>Robarts Research Institute, London, ON, Canada; ²The University of Western Ontario

A methodology to detect the on-resonance paramagnetic chemical exchange effects (OPARACHEE) of a PARACEST contrast agent: Tm³⁺-DOTAM-Glycine (Gly)-Lysine (Lys) in a mouse brain tumor model was developed. The OPARACHEE effect was isolated from the relaxation effects induced by the PARACEST agent using a control image and an OPARACHEE image. Isolated OPARACHEE contrast (1-3%) was observed in all animals. Immediately after contrast agent injection OPARACHEE contrast was observed and maintained at 1~2% in the hour following injection.