Formats for submission of projects

Finally, implementation of this work will bring together material science, engineering and biological sciences in a unique knowledge-sharing and developmental platform.

300. BUDGET ESTIMATES: SUMMARY

N n	Item	BUDGET					(in lakhs of rupees)
		1^st  Year	2^nd Year	3^rd  Year			Total
A.	Recurring	N	Nn				N
N	1. Salary	N 4.32	4.44.32	4.8			N9. 13.44
N	2. Consumables	222.5	N n2.5	1.0			N 6.05.
N	3. Travel	N.0.250	N 0.30	0.25			N 0.8
N	4. Contingency	N 0.4	N 0.3	0.3			N 1.0
B.	Equipment	28.2	20 0	0			N 28.2
N	Total (A+B) Grand total (A+B+20% overhead) Total FEC*	N	N				Rs. 49.44 lakhs Rs. 59.328 lakhs28 47,774 US$ and 3,257 Euro

*FEC - Foreign Exchange Component 1 US$ = 53 Rs., 1 Euro = 70 Rs. as of Dec., 2011.
310. BUDGET FOR SALARIES/WAGES

		1st Year	2nd Year	3rd Year	Total (Rs.)
Designation & number of persons	Monthly Emoluments	18,000 per month x 2 students for doing PhD	18,000 per month x 2 students for doing PhD	20,000 per month x 2 students for doing PhD
Total		4,32,000	4,32,000	4,80,000	13,44,000

311. Justification for the manpower requirement

The salaries will be used for recruiting two students to do PhD (Rs. 18000/- per month, per student x 12 months = Rs. 2,16,000/- per students annually, for the first two years and, Rs. 20000/- per month, per student x 12 months = Rs. 2,40,000/- per students annually, for the third year). One of them will work on nanoparticle modeling and simulation techniques, developing new hybrid models to address issues of anisotropic particles, reaction and nucleation in large single molecules like dendrimers and specifically adapting existing models for optimizing Ag-AC systems.
The second student will exclusively focus on synthesis and characterization of Ag nanoparticles of controlled size, shape and composition. Colloidally stable and biocompatible Ag samples will be embedded by him to make Ag-GC composite for antibacterial tests, leading to a filter design. A combined synergistic approach between these two students and the PI, handling modeling and experimental parts of the work, respectively, will accelerate towards the goals of the work.
320. BUDGET FOR CONSUMABLE MATERIALS

		BUDGET
Item		1st Year	2nd Year	3rd Year	Total (Rs.)
	Q*	-	-	-	-
	B**	2,50,000	2,50,000	1,00,000	6,00,000
	F***	-	-	-	-
Total	B	2,50,000	2,50,000	1,00,000	6,00,000
	F	-	-	-	-

*Q: Quantity or number, **B: Budget, **F: Foreign Exchange Component in US$

321. Justification for consumables

The consumables budget is required for purchasing chemicals, solvents, surfactants, polymers, additives, liquid nitrogen, nitrogen and helium gas, glassware and plasticware.
330. BUDGET FOR TRAVEL

		BUDGET
		1st Year	2nd Year	3rd Year	Total (Rs.)
	Travel (only inland travel)	25,000	30,000	25,000	80,000

331. Justification for travel

Travel amount is required for presenting research results in conferences and DST review meetings. On an average, results will be presented annually in one or two national conferences.
340. BUDGET FOR OTHER COSTS/CONTINGENCIES

		BUDGET
		1st Year	2nd Year	3rd Year	Total (Rs.)
	Other costs/Contingency Costs	40,000	30,000	30,000	1,00,000

341. Justification for contingency

Contingency amount is needed for covering stationary, printing, photocopying and other incidental charges. It will in addition be utilized for other things like, buying small accessories, repair of small equipments or charge for instrumental analysis in various instrumental and analytical facilities.

350. Detailed Budget for Equipment

Sr. No.	Generic name of the equipment	Imported/indigenous	Estimated costs (in lakhs of rupees, L)	Spare time for other users (in %)
1. 2. 3. 4. 5. 6.	High temperature furnace with controller Fluorescence spectrometer Homogenizer Constant temperature circulator Refrigerator Sonicator	Imported Imported Imported Imported Local Local	3.80 L 20% 14.87 L 30% 6.65 L 30% 2.28 L 30% 0.25 L 0.35 L 30%

351. Justification for the proposed equipment
High temperature furnace with controller: Synthesis and impregnation of nanoparticles requires heating to a high temperature with a controlled heating rate and maintaining a constant temperature during reaction. A furnace with a PID controller is necessary for this.
Fluorescence spectrometer: It is needed to measure cell viability. For example, the signal from SYTOX green nucleic acid stain is proportional to the number of cells that have lost membrane integrity. So it will directly quantify the cell death or inactivity and rank the relative efficacy of antibacterial properties of different Ag-GC composites. In addition, an anionic dye (sulforhodamine 101) measures total protein by forming complexes with basic amino acid residues. Fluorescence spectrometry technique can be used to detect changes in cell size or population over a range of 100 to more than 100,000 cells.
Homogenizer: This is required for making dispersions and emulsions of two immiscible liquids, which will be directly useful in synthesizing Ag nanoparticles and for further impregnation. For example, a homogenizer can produce emulsions of quite monodisperse droplets of a well-controlled droplet diameter. Thus one can systematically vary droplet diameter and hence diameter of nanoparticles (synthesized in emulsion droplets), to study its effect on amount of Ag impregnation and its antibacterial properties.
Constant temperature circulator: One can maintain reaction temperature between -20 °C to 100 °C, by using a circulating heating/cooling water bath, thereby studying the effect of reaction temperature on nanoparticle synthesis. Lower temperature synthesis will result in almost no aggregation of particles, enhancing surface area of Ag for enhanced contact with bacteria.
Refrigerator: Many chemicals and samples for further analysis (like dendrimer, nanoparticle dispersions) need controlled temp. storage at low temperatures. So a refrigerator is essential.
Sonicator: It is necessary for making stable dispersions of particles for any analytical sampling, and TEM sample preparation for imaging particle size and shape.

4
9.Explore bacteria-carbon-metal contact

10. Time Schedule of Activities through Bar Diagram

8. Address flow issues in small water disinfection unit

7. Optimization and adhesion enhancement of Ag in AC

6. Modeling of size, shape and composition and validation

5. Synthesis in surface modified AC and investigation of the enhancement of E. Coli removal

4. Synthesis of dendrimer and emulsion template Ag in AC and anti-microbial property testing

3. Synthesis of citrate stabilized Ag in AC and anti-microbial property testing

Activity

2. In-situ synthesis of Ag in AC and anti-microbial property testing

1. Literature Review & Equipment Installation

420. List of facilities being extended by the parent institution for the project implementation

A: Infrastructural Facilities

Sr. No.	Infrastructural Facility	Yes/No/Not required Full or sharing basis
1.	Workshop facility	Available but not required
2.	Water & Electricity	Yes
3.	Laboratory Space/Furniture	Yes
4.	Power Generator	Yes
5.	AC Room or AC	Yes
6.	Telecommunication including email & fax	Yes
7.	Transportation	Available but not required
8.	Administrative/Secretarial support	Yes (sharing)
9.	Information facilities like Internet/Library	Yes (sharing)
10.	Computational facilities	Yes (sharing)
11.	Animal/Glass House	Not required
12.	Any other special facility being provided	Central equipment facility, like XRD, TEM, SEM, EDAX, BET, FTIR

B. Equipment available with the Institute/Group/ Department for the project

Equipment available with	Generic Name of Equipment	Manufacturer, Model & Year of purchase	Remarks including accessories available and current usage of equipment
PI & his group	Photo Resist Spinner Electronic Balance Fume Hood High Speed Centrifuge Rotary Vacuum Pump Laboratory stirrers Laser Particle Sizer Plasma cleaner Ellipsometer	Ducom, 2007 Merck, 2008 Labguard, 2009 Remi Instruments, R-24, 2007 Vacuum Technique, 2007 Remi Instruments Malvern, 2009 Harrick Plasma, 2009 J A Woollam, 2009	Regular usage -do- -do- -do- -do- -do- -do- -do-
PI's Department	UV-Visible Spectrophotometer Surface Area & Pore Size Analyzer	Perkin Elmer, 2009 Micromeritics, ASAP 2020	Available Available
Other department (within IIT Bombay)	Transmission Electron Microscope Scanning Electron Microscope X-Ray Diffractometer	Philips CM 200 JEOL, JSM 6400 Panalytical, Rigaku	Available on booking -do- -do-

430. Detailed Bio-data of the Principal Investigator