Formulaire pour demande d' homologation cati

2. From AcypiCyc to ArthropodaCyc
CycADS has been successfully used to generate AcypiCyc1, the pea aphid BioCyc database, and we decided to build a metabolic network database for other arthropods, for which the genome sequence is available. We kept the same workflow parameters for collecting data from all used annotation methods (Blast2GO, KAAS, PRIAM). The generated ArthropodaCyc2 database includes, at present, metabolic reconstructions for 11 arthropods: Acyrthosiphon pisum, Aedes aegypti, Anopheles gambiae, Apis mellifera, Culex quinquefasciatus, Daphnia pulex, Ixodes scapularis, Nasonia vitripennis, Pediculus humanus corporis, Tribolium castaneum and Drosophila melanogaster (for this last species, both the CycADS version and the FlyCyc database manually curated by the FlyBase team are available). Collecting and organizing information into databases is useful for the researchers studying the metabolism of their newly sequenced model organisms (more arthropod genomes will be sequenced in the near future through the i5K Arthropod Sequencing initiative), and it allows them to better understand different aspects of arthropod biology through comparative studies.

Thanks to the CycADS software, we included, in each database, information on annotation sources and links to genomics databases (including AphidBase, BeetleBase, VectorBase, Hymenoptera Genome Database, FlyBase and wFleaBase). Future plans include adding other sequenced genomes to ArthropodaCyc and the generation of another BioCyc-like database centred on the arthropod endosymbiosis for which both host and symbiont genomes have been sequenced (a beta version of ArtSymbioCyc is already available).