Figure 3: Reverse phase HPLC profile of monohydroxy-derivatives of linoleic (HODEs) and arachidonic (HETEs) acids of biological relevance, with detection at 235 nm. Sensitivity can be evaluated from the less represented metabolite, 15-HETE, as the signal corresponds to around 10 pmol.
Figure 4: Prostanoid generation from arachidonic acid. PGG2 and PGH2 from arachidonic acid are shown as an example of what may occur from dihomogammalinolenic and eicosapentaenoic acids, with the production of PGG1/H1 and PGG3/H3, respectively. The formation of the other prostanoids also occurs from the latter two PUFA, except for PGI1 that cannot be formed because of the lack of double bond at carbon 5. The same metabolic pathways have been reported for dihomo-arachidonic or adrenic acid with the products called dihomo-prostanoids.
PG: prostaglandin; Tx: thromboxane; PG-S: PG synthase; PGH-S is a bi-functional enzyme protein with two activities: cyclooxygenase producing PGG, and hydroperoxidase converting PGG into PGH.
