N
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Topics
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Hours
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1
|
Microbiology laboratory. The methods of microbiology investigation. Microscopes. The working manner with microscopes
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2
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2
|
Microscopic method. Smears preparation from different pathological specimens and pure cultures. Morphology and classification of bacteria. Aniline stains. The simple stain
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2
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3
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Structure of bacterial cell. Gram and Neisser stains
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2
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4
|
Structure of bacterial cell. Acid-fast bacteria and Ziehl Neelsen stain. Spores and spore stain
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2
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5
|
Structure of bacterial cell. Capsule. Gins-Burry stain. Burry stain. Flagella. Investigation of bacterial motility. Vital staining
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2
|
6
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Morphology and classification of Spirochetes, Rickettsiae, Chlamydia, Mycoplasma and Actinomyces (Giemsa stain)
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2
|
7
|
Morphology and classification of fungi protozoa and viruses
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2
|
8
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Metabolism and nutrition of microorganisms, nutrition media. Action of physical and chemical factors on microorganisms. Sterilization and disinfection
|
2
|
9
|
Respiration and multiplication of microorganisms. Cultivation of aerobes and anaerobes
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2
|
10
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Bacteriological method. Isolation of the pure culture of aerobes and anaerobes (the first day)
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2
|
11
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Isolation of the pure culture of aerobes and anaerobe (the second day). Cultural character of microorganisms
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2
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12
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Isolation of the pure culture (the third day). Biochemical character of microorganisms
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2
|
13
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Cultivation of viruses, rickettsia and chlamydia. Bacteriophages. Obtaining and studying of bacteriophages
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2
|
14
|
Obtaining and classification of antibiotics. Antibiotic sensitivity tests
|
2
|
15
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Ecology of microorganisms. Microflora of air, water, soil and human organism
|
2
|
16
|
Conclusion lesson
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2
|
17
|
Infection. Laboratory animal inoculation. Determination of pathogenecity and virulence of microorganisms
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2
|
18
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Immunity. Non specific protection factors of host. Phagocytosis. Determination of phagocytes’ activity
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2
|
19
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Serological reactions. Agglutination reactions. Hemagglutination reactions, passive hemagglutination test. Coombs test
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2
|
20
|
Precipitation reactions. Neutralization tests (toxin neutralization and virus neutralization tests). Lysis reactions. Complement fixation test (CFT)
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2
|
21
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Immunofluorescence method. Enzyme linked immunosorbent assay (ELISA). Radioimmunoassay method. Immunoblotting. Polymerase chain reaction (PCR)
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2
|
22
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The principles of immunoprevention and immunotherapy. The vaccine and antiserum
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2
|
23
|
Conclusion lesson
|
1
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