| Dependent variables determined
The vase life of gladiolus cut flowers was determined by containing the number of florets open, length of stem, diameters inflorescence, was also recorded change carbohydrate in leaf and petal flower although instrument content chlorophyll in leaf. Soluble carbohydrate were extracted from leaf and petal (days 1, 5, 10) 0/25 gr chopped material of perianth tissue was fixed in the ethanol, the material was macerated and centrifuged (3500x, 10 min) the supernatants were pooled and use for the estimation of carbohydrate. Carbohydrate were estimated by the method of pakvain and lechacer [1979] using Antron as standard Soluble proteins were extracted from leaves (1, 5, 10 days) and petal in an extraction buffer (0,01 Mtris-HCL…) and protein assay was carried out according to method of Bradford, content chlorophyll in leaf: At each stage (1, 5, 10 d) 0/5 gr chopped material of perianth tissue was fixed in water deionizer, the tissue was homogenized in 10 ml acetone the material was macerated and centrifuged (3500X , 10 min) the supernatants were pooled and use for the estimation of, Absorbance of extracts were measured using a WAPS 105 spectrophotometer according to (Ausati 1980) the leaf chlorophyll content was determined as absorbance of these extracts at 663 and 645 nm [Ausati, 1980]. The following equation was used to calculate the relative total chlorophyll content [Ausati, 1980].
Data analysis
Data were analysis using one – way ANOVA with the generalized liner model procedure of sas (Version 9.1, sas institute Inc., carry, NC, USA). Where significant (P≤0.05) treatment effects were determined by ANOVA, data means were separated by LSD test at P=0.05.
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