Figure 2: Expression of genes implicated in mitochondrial biogenesis and in mtDNA replication. mRNA levels of mitochondrial biogenesis (A) and mtDNA replication (B) genes, determined by real-time RT-PCR, in gastrocnemius muscle of SD and HFHSD mice, after 4 and 16 weeks of diet (n=6). Results were expressed as fold change versus the SD conditions set to one unit (dotted line). * p< 0.05. PGC1: PPAR gamma coactivator 1, NRF: nuclear respiratory factor, mtTFA: mitochondrial transcription factor, ERRestrogen-related receptor , Mfn2: mitofusin 2, POLG: polymerase gamme, SSBP1: single strand binding protein.
Figure 3: Alterations in mitochondrial structure in skeletal muscle of 16 week HFHSD-fed mice. A-B: Transmission electronic microscopy images (magnification x 25,000 (A) and x 100,000 (B)) of subsarcolemmal and intermyofibrillar mitochondria in gastrocnemius muscle of 16 weeks SD and HFHSD-fed mice. C: Quantification of subsarcolemmal and intermyofibrillar mitochondria area in gastrocnemius muscle of 16 week HFHSD mice (analysis of 5 images in 3 mice per group). Results were normalized by the mean value of the 16 week SD condition set to one unit.* p<0.05, **p<0.01.
