The effects of environmental factors on the toxin producing genes in cyanobacteria
Water is an indispensable source for life. However, if domestic and industrial wastes reach to the water sources like rivers, lakes and seas, without enough treatment serious ecological problems will arise. Eutrophication as a result of deterioration in water quality and formation of algal blooms causes fish deaths and affects public health negatively because of toxicity problems. Microcystis is the most common cyanobacteria in fresh waters and is usually toxic. Determination of microcystin producing organisms and toxin amounts in water sources, that are used for drinking, irrigation, swimming and other recreational activities is very important to be protected against deadly effects of microcystin. Nowadays, many countries monitor cyanobacteria cell density and toxin concentrations in their water sources. Determination the effects of environmental factors on toxin production of cyyanobacteria in experimental conditions faciliated to understand their ecological and physiological roles in their habitat. The aim of this study is to determine the changes in the expression of toxin genes depending on stress factors. Light and iron concentration will be used as a stres factors on Microcystis aeruginosa in cultured conditions. Real-time PCR was used to detect the changes in the expression of toxin genes according to different light and iron concentration. The result was revealed that iron starvation and continuous light stress enhanced mcyB gene expression and as a result microcystin production, however the growth of M. aeruginosa cells were affected negatively.
GÜRÜN Sevan
Danışman : Prof. Dr. Gülşen ALTUĞ
Anabilim Dalı : Su Ürünleri Temel Bilimleri
Programı : Deniz Biyolojisi
Mezuniyet Yılı : 2014
Tez Savunma Jürisi : Prof. Dr. Gülşen ALTUĞ
Prof. Dr. Bayram ÖZTÜRK
Prof. Dr. Meral BİRBİR
Prof. Dr. Ayşe OGAN
Prof. Dr. Nuray BALKIS
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