Traditional Posters: Miscellaneous

Haoyang Xing^1,2, Hao Shen³, Hehan Tang¹, Xiaoqi Huang¹, Qiyong Gong¹, Xiaohong Joe Zhou<sup>2,4

1</sup>Center for MR Research, West China Hospital, Sichuan University, Chengdu, Sichuan, China; ²Center for MR Research, University of Illinois Medical Center, Chicago, IL, United States; ³GE Healthcare, Beijing, China; ⁴Departments of Radiology, Neurosurgery, and Bioengineering, Chicago, IL, United States

Characterization of phosphocreatine (or PCr) in biological tissues can provide valuable information on tissue physiology and metabolism and may lead to sensitive disease markers. We have developed 2D and 3D spectrally selective PCr imaging techniques on a human 3T MRI scanner. Low resolution PCr images (32x32) can be obtained in 10.8 minutes with an SNR of ~4, boding well for further developing these techniques for human applications.

996. in Vivo Molecular Imaging of Angiogenesis in Murine Urogenital Organs with Targeted Perfluorocarbon Nanoparticles and ¹⁹F ultrafast Turbo Spectroscopic Imaging (F-uTSI)

Muhammed Yildirim^1,2, Anke De Vries², Monique Berben¹, Caren van Cammen³, Klaas Nicolay², Holger Gruell^1,2, Rolf Lamerichs<sup>1

1</sup>Philips Research, Eindhoven, Netherlands; ²Biomedical NMR, Faculty of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, Netherlands; ³Maastricht University, Maastricht, Netherlands

MR molecular imaging method based on 19F targeted nanoparticles and novel imaging sequences promises potential use in examining angiogeneic processes taking place in the ovaries and the endometrium in situ. Angiogenesis in the urogenital organ of the femal plays a vital role endometriosis, endoendometrial cancer and infertility.

997. ¹⁹F Magnetic Resonance Quantification of SiRNA Delivery Via Perfluorocarbon Nanoparticle Emulsions

Jacob Wheatley Myerson¹, Megan M. Kaneda¹, Gregory M. Lanza^1,2, Samuel A. Wickline<sup>1,2

1</sup>Biomedical Engineering, Washington University, Saint Louis, MO, United States; ²Department of Medicine, Washington University, Saint Louis, MO, United States

Perfluorocarbon nanoparticles were functionalized to deliver VCAM-1 siRNA to human melanoma cells. Delivery was quantified via ¹⁹F magnetic resonance spectroscopy. For future in vivo studies, an MR-trackable delivery agent would aid in the determination of localization of siRNA delivery to specific tissues.

998. The Loss of Sodium Homeostasis and Apoptosis During Rodent Glioma Chemotherapy

Victor D. Schepkin¹, Cathy W. Levenson², Fabian F. Calixto-Bejarano², William W. Brey¹, Petr L. Gor'kov<sup>1

1</sup>CIMAR, NHMFL/FSU, Tallahassee, FL, United States; ²College of Medicine, FSU, Tallahassee, FL, United States

The goal of the present study was to assess the hypothesis that an in vivo increase of intracellular sodium is one of the first and a crucial stage during cancer therapy. Experiments were performed using high resolution sodium and diffusion MRI at 21.1T and rodent glioma model. During efficient BCNU chemotherapy, tumor sodium reaches a plateau indicating a complete loss of Na homeostasis at day 4 following the initiation of therapy. Dose dependent responses of intracellular sodium can serve as a very early biomarker for the onset of apoptosis and forecast tumor elimination.

999. In Vivo Multicolor Imaging of Perfluorocarbon Emulsions Using Ultrafast Spectroscopic Imaging (F-UTSI)