Traditional Posters: Miscellaneous
Hyperpolarized Carbon-13 & Other Nuclei
Yüklə 355,76 Kb.
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| Hyperpolarized Carbon-13 & Other Nuclei
Hall B Wednesday 13:30-15:30 1012. Metabolic Imaging of the Perfused Rat Heart Using Hyperpolarized [1-13C]Pyruvate Philip Lee1, Marie Schroeder2, Daniel Ball2, Kieran Clarke2, George Radda1, Damian Tyler2 1Singapore Bioimaging Consortium, Biomedical Sciences Institute, Singapore, Singapore; 2Department of Physiology, Anatomy & Genetics, University of Oxford, United Kingdom Imaging of cardiac metabolism using 13C-MRS is currently hindered by both a low sensitivity and a low natural abundance of 13C. The recent development of liquid state Dynamic Nuclear Polarization (DNP) techniques has dramatically increased the signal available from 13C-MRS experiments and has opened up new possibilities for metabolic imaging of the heart. By injecting hyperpolarized 13C-labeled pyruvate into a perfused rat heart, followed by high spatial resolution chemical shift imaging (CSI) during an optimum acquisition window, we were able to image the bio-distribution of lactate, bicarbonate and alanine within 46 s. 1013. Investigation of Hepatic Metabolism of DNP Hyperpolarized 1,4-13C2 Succinate Jeremy W. Gordon1, Kang Wang1, Sean B. Fain1,2, Ian J. Rowland2 1Medical Physics, University of Wisconsin-Madison, Madison, WI, United States; 2Radiology, University of Wisconsin-Madison, Madison, WI, United States This work describes the successful hyperpolarization of 1,4-13C2 succinate using dynamic nuclear polarization (DNP). By optimizing the solubility of succinic acid in aqueous solution, levels of solid state similar to that of pyruvate can be reproducibly obtained. 3.0M solutions of hyperpolarized succinate were utilized to investigate hepatic metabolism in vivo. 13C spectra and imaging show that the biodistribution of succinate within the liver is homogenous. No metabolites were observed in the time frame of the hyperpolarized experiments. Metabolites were also not observed in ex vivo organ homogenates. 1014. Imaging TCA Cycle Metabolism by PHIP Hyperpolarization of 1,3C Succinate In Vivo Niki Zacharias Millward1, William Perman2, Brian Ross1, Pratip Bhattacharya1 1Enhanced Magnetic Resonance Laboratory, Huntington Medical Research Institutes, Pasadena, CA, United States; 2Saint Louis University In vivo metabolic imaging of reactions in the Krebs TCA cycle using hyperpolarization was performed using 13C deuterated fumarate that was hydrogenated to 1-13C succinate and hyperpolarized to ~8% by PHIP. On tail-vein injection of hyperpolarized succinate in tumor-bearing mice, hyperpolarized metabolic products were detected with 20,000 fold increased sensitivity over 3-5 minutes. The metabolic fate of hyperpolarized succinate differed in two tumors: in RENCA renal carcinoma metabolic products malate, fumarate, glutamate and citrate were defined, and in Lymphoma A20 the metabolic products were limited to malate. These differences are tentatively assigned to the presence of hypoxia inducing factor HIF1α. 1015. Exploring Multi-Shot Non-CPMG for Hyperpolarized 13C Metabolic MR Spectroscopic Imaging Yi-Fen Yen1, Patrick Le Roux2, Dirk Mayer3,4, Atsushi Takahashi1, James Tropp1, Dan Spielman3, Adolf Pfefferbaum4,5, Ralph Hurd1 1Global Applied Science Laboratory, GE Healthcare, Menlo Park, CA, United States; 2Global Applied Science Laboratory, GE Healthcare, France; 3Radiology, Stanford University, Stanford, CA, United States; 4Neuroscience Program, SRI International, Menlo Park, CA, United States; 5Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, United States We explored the feasibility of a multi-shot non-CPMG sequence for hyperpolarized 13C metabolic imaging. The sequence is designed to stabilize the longitudinal magnetization while keeping the transverse magnetization refocused, permitting echo-train readouts following multiple low-flip-angle excitations. Thus, acquisition strategies can be developed to take advantage of the long T1 and T2 relaxation times of hyperpolarized 13C metabolites. We demonstrate two of the potential applications, 2D T2 mapping and 3D MR spectroscopic imaging, on 13C phantoms and animals with hyperpolarized 13C-pyruvate injections. 1016. Single-Shot Spiral Chemical Shift Imaging in the Rat In Vivo with Hyperpolarized [1-13C]-Pyruvate Dirk Mayer1,2, Yi-Fen Yen3, Atsushi Takahashi3, James Tropp3, Brian K. Rutt2, Ralph E. Hurd3, Daniel M. Spielman2, Adolf Pfefferbaum1,4 1Neuroscience Program, SRI International, Menlo Park, CA, United States; 2Radiology, Stanford University, Stanford, CA, United States; 3GE Healthcare, Menlo Park, CA; 4Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, United States Using undersampled 13C spiral chemical shift imaging (CSI) in combination with a high-performance gradient insert we achieved single-shot hyperpolarized 13C metabolic imaging of the rat in a clinical 3T MR scanner. With an acquisition time of 125 ms, the method produced metabolic images of pyruvate and its metabolic products lactate and alanine with similar quality as with conventional CSI using phase encoding, despite an almost 200-fold reduction in acquisition time. Because the longitudinal magnetization does not recover in hyperpolarized MRI, there is no intrinsic SNR disadvantage of the faster imaging method. 1017. Double Spin-Echo Spiral Chemical Shift Imaging for Rapid Metabolic Imaging of Hyperpolarized [1-13C]-Pyruvate Yüklə 355,76 Kb. Dostları ilə paylaş:
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