ODTP-P4 ANIDRASE CARBÔNICA VI NA SALIVA E BIOFILME: REVISÃO DE LITERATURA
Faculdade de Odontologia de Piracicaba - Unicamp
Autores: Picco DCR*, Nobre-dos-Santos M
Apoio Finaceiro: FAPs - Fapesp N° 2012/02886-3
Carbonic anhydrase VI in saliva and plaque: a literature review
Abstract: Carbonic anhydrase VI (CA VI) is the only way of isoenzyme which can be secreted into the saliva in order to provide a higher buffering capacity, and promote the retention of HCO3- level in salivary glands. The high pH of the saliva would be required to maintain the appropriate medium for the multiplicity of enzymes that are present, playing a remarkable role in protecting teeth against tooth decay. This literature review aimed to discuss the mechanisms by which the CA VI in saliva and biofilm acts in the buffering of acids present in the oral environment as well as the scientific evidence on the relationship between the expression/activity of CA VI in saliva or plaque and caries experience. The buffer effect of isoenzyme derives from the fact that CA VI catalyze the reversible reaction of carbon dioxide in the reaction CO2 + H2O ↔ H + + HCO3-. Furthermore, the CA VI is adsorbed on the tooth surface remaining enzymatically active. The analysis of the literature shows conflicting results between the concentration of CA VI in saliva/plaque and caries experience. Furthermore, there is evidence that a high concentration of CA VI saliva/biofilm may not necessarily mean that all isoenzyme is active and thus capable of neutralizing the acid after a cariogenic challenge. There are no reports on the possible relationship between the concentration and activity of AC VI in saliva and dental plaque.
ODTP-P5 APARELHO MANTENEDOR DE ESPAÇO COLADO: UMA ALTERNATIVA PARA PERDA PRECOCE DE DENTES DECÍDUOS - RELATO DE CASO CLÍNICO
Faculdade São Leopoldo Mandic
Autores: LamarãoMCM, VilhenaDHM, AlmeidaJT, Vasconcelos A, GirãoDC, Moura BF, BoniniG
Appliance space maintainer glued: an alternative to early loss of deciduous teeth - Clinical Case Report
Abstract: The early loss of primary teeth can cause a lot of harm, leaving sequelae that unbalance physiology of the oral cavity.The space maintainers are devices that can be used when a primary tooth is lost prematurely in order to preserve the integrity of the occlusion, keep teeth involved in their respective positions, to preserve space resulting from the premature loss of deciduous teeth and allow the permanent tooth erupcione in normal position.The following work aims to present the case of a 7 year old, male white, convex profile, mixed dentition, Class I molar relationship, whose chief complaint was "stuck tooth" (according to information gathered). On clinical examination, there was an open cavity in the second lower right deciduous molar (85) and fistula. The element has undergone endodontic treatment, having unsatisfactory biological response.We opted for the element extraction and subsequent installation of a space maintainer appliance glued (AMEC), to wait for the eruption of permanent fixture. The maintainer of this selection was made on the basis of their properties, because it has advantages such as low cost, ease of preparation, good resistance, easy to clean and does not require laboratory phase and is indicated in cases of early loss of a single element dental later.According to the established schedule for the case in question, was performed prior to extraction molding for obtaining model and fabrication of orthodontic wire loop with 0.7. To install the maintainer etching was done with 37% phosphoric acid in the vestibular region 46 of the element 84 and, after 15 seconds acid was removed by washing with water while application of the product, then application was made Single Bond and curing light for 20 seconds, then by inserting the composite Filtek Z250 buccal teeth mentioned above, and subsequently positioning the bent wire on the resin and curing for 40 seconds. The unit should be kept until the eruption of the tooth successor. Thus, it can be concluded that the AMEC can be a viable option for cases of early loss of deciduous molars and can obtain satisfactory results and success.
ODTP-P6 APLICAÇÃO DE MATERIAIS RESINOSOS COMO MEDIDA PREVENTIVA EM ESMALTE INICIALMENTE ERODIDO, SUBMETIDO A DESAFIO EROSIVO – ESTUDO IN VITRO
Faculdade de Odontologia de Bauru - Universidade de São Paulo (USP)
Autores: Oliveira GC*, Boteon AP, Ferrairo BM, Gonçalves PSP, Alencar CRB, Honório HM, Rios D.
Application of resin-based materials as a preventive measure for eroded enamel, subjected to erosive challenge - in vitro study.
Objective: The aim of this study was to evaluate the effect of resin-based materials application on enamel with initial erosion lesion on the prevention of dental wear. The application of materials was performed with and without prior enamel surface conditioning. The bovine enamel blocks were immersed in HCl 0. 01 M, pH 2.3 per 30 seconds for formation of the initial erosion lesion. Then the blocks were random divided in 9 groups according to the material applied (n=12): EC-control without treatment, EH-sealant Helioseal Clear®, EA-self-etch adhesive Adhese®, ET-conventional adhesive Tetric N-bond®, EI-infiltrant Icon®, EHs, EAs, ETs groups received the same materials without surface conditioning. After the treatments the specimens were immersed in HCl for 2 min, followed by immersion in artificial saliva for 120 min, 4xdays, during 5 days (erosive cycling). Analysis of enamel wear and material thickness were conducted using perfilometry and the results submitted to Kruskal-Wallis and Dunn test (p>0.05). The groups EH, EA, EI, ETs and EIs did not show enamel alterations, being effective in inhibiting erosion progression. Based on the results of this study, it is concluded that the application of pits and fissure sealant and infiltrant (regardless of the surface condition) on eroded enamel was effective in inhibit the progression of dental erosion.
ODTP-P7 ARTROGRIPOSE MÚLTIPLA CONGÊNITA ASSOCIADA À SÍNDROME DO PTERÍGIO MÚLTIPLO - RELATO DE CASO
Faculdade de Odontologia de Araraquara - Universidade Estadual Júlio de Mesquita Filho (UNESP)
Autores: Oliveira RVD*, Brighenti FL, Jeremias F
Arthrogryposis multiplex congenita associated to Multiple Pterygium Syndrome: case report
Abstract: Arthrogryposis multiplex congenita associated to Multiple Pterygium Syndrome is a rare, autosomal recessive disease characterized by nonprogressive articular contractures and skinfolds. It causes movement limitation, stiffness and periarticular thickening. The aim of this study is to describe a case report of a patient with this condition assisted at Araraquara Dental School – UNESP. D.R.S.N., male, 10 years old showed joints distortion of upper and lower limbs and of the hip, limitation of secondary movement, muscle weakness, postural problems and short stature. The facial characteristics include: convex profile, vertical growth, elongation of the lower third, retruded chin and smile line inverted. During clinical exam, it was observed interincisaldiastema, anterior open bite, atypical swallowing with lingual inter- position, severe maxillary atresia, mouth breathing, limited mouth opening and teeth crowding. The observed modifications in face and in oral cavity may hamper oral hygiene and dental treatment. The extensive esthetic and functional committed emphasize the importance of an early transdisciplinary approach in order to improve life quality of this patients. Very little is known about facial-cranium-dental peculiarities of this condition, which justifies its study.
ODTP-P8 ASSOCIAÇÃO ENTRE DIFERENTES TIPOS DE MÁ OCLUSÃO E IMPACTO NA QUALIDADE DE VIDA DE CRIANÇAS PRÉ-ESCOLARES
Autores: Ramos-Jorge J, Sá-Pinto AC*, Motta-Rêgo T, Pereira CLS, Paiva SM, Marques LS, Ramos-Jorge ML
Association between different types of malocclusion and impact in quality of life of preschool children
Objective: The aim of the present study was to evaluate the association between different types of malocclusion and impact in quality of life among preschool children and their families. Methods: A population-based cross-sectional study was carried out with 451 children between three and five years of age. A clinical exam was performed for the evaluation of malocclusion: anterior and posterior open bite, anterior and posterior crossbite and increased overjet (>3 mm). Dental caries and traumatic dental injury was evaluated as possible confounding variables. Parents/caregivers answered the Child Impact Section and Family Impact Section of the Early Childhood Oral Health Impact Scale (ECOHIS) for the assessment of Oral Health-Related Quality of Life (OHRQoL) and a questionnaire on socioeconomic and demographic characteristics. Data analysis involved descriptive statistics, the chi-square test, Mann-Whitney test and hierarchically adjusted Poisson regression models. Results: The prevalence of malocclusion was 28.4%. The most frequent conditions were posterior crossbite (20.4%), anterior open bite (9.5%) and increased overjet (8.4%). A significant association was found between the anterior open bite and OHRQoL in terms of impact on both the children and family (p<.001). The adjusted analysis confirmed the association between anterior open bite and a negative impact on quality of life (RR = 2.55; 95% CI: 1.87 to 3.47; p < .001). Conclusion: Anterior open bite was associated with a negative impact in the quality of life of preschoolers.
ODTP-P9 AVALIAÇÃO DA DENSIDADE TUBULAR E DUREZA SUPERFICIAL EM DENTINA DECÍDUA
Faculdade de Odontologia da Universidade de São Paulo
Autores: Calvo AFB*, Tedesco TK, Domingues GG, RaggioDP
Evaluation of tubular density and hardness in primary dentin
Objective: Based on the difficulty for acquisition human teeth for research, the possibility of using more fragments of the same specimen would be an option to reduce the total number of teeth. The aim of the present study was to evaluate properties of surface hardness and tubule density contiguous surfaces of primary molars, in order to enable the use of parts of the same tooth for in vitro studies. Ten first primary molar were sectioned perpendicular to the long axis, with double face diamond disc, in occlusal third. Resulting in a top and bottom substrates. The surface hardness was measured with Knoop (25g/5sec) indenter and the density of dentinal tubules was evaluated at 100x magnification in confocal laser microscopy. Two trained examiners had analyzed the images. The mean (± standard deviation) of superficial hardness were 49.63 (± 5.65) and 51.11 (± 9.04) for the upper and lower portions, respectively. The mean tubular density (± standard deviation) of dentinal tubules in the contiguous surfaces were 33,181.71 (± 7,022.85) for the superior portion and 27,664.22 (± 6,620.83) for the inferior. It can be concluded that there is no difference between the values of surface hardness and tubule density and thus contiguous surfaces of the same tooth could be used in in vitro studies.
ODTP-P10 AVALIAÇÃO DA PERFORMANCE MASTIGATÓRIA E FORÇA DE MORDIDA EM CRIANÇAS E ADOLESCENTES COM DISFUNÇÃO TEMPOROMANDIBULAR
Faculdade de Odontologia de Piracicaba - FOP - UNICAMP
Autores: Marquezin MCS*, Kobayashi FY, Montes ABM, Gavião MBD, Castelo PM
Evaluation of masticatory performance and bite force in children and adolescents with temporomandibular dysfunction
Objective: Temporomandibular dysfunction (TMD) involves changes in the structure and function of the masticatory muscles and temporomandibular joints. Thus, the aim of this study was to evaluate the masticatory performance (MP) and bite force (BF) in TMD subjects. TMD group comprised 50 subjects (age 10.94 ±2.02 years) and 50 subjects comprised the control group (10.8 ±2.13), matched for gender, stage of dentition and orthodontic treatment need. MP was evaluated by the individual’s ability to comminute an artificial test food in order to determine median particle size (X50) and distribution of particles in the different sieves (“b”). BF was determined using a digital gnatodynamometer with fork strength of 10 mm. TMD was evaluated using the Research Diagnosis Criteria. The results were submitted to descriptive statistics, Shapiro Wilk test, "t" test, Mann Whitney and Wilcoxon. TMD group median values (interquartile range) for X50 and "b" were 4.68 (2.09) and 2.20 (0.44); control group values were 4.96 (2.16) and 2.10 (0.53), with no statistical difference between the groups. The mean values (±SD) for BF were 355.60N (±86.12) and 345.18N (±111.42) for TMD and control groups, respectively. There was also no significant difference between groups for BF. In conclusion, the presence of TMD did not alter the masticatory parameters evaluated in the sample studied.
ODTP-P11 AVALIAÇÃO DA RELAÇÃO ENTRE FORÇA DE MORDIDA E ÍNDICE DE MASSA CORPORAL EM CRIANÇAS JOVENS: ESTUDO PRELIMINAR
Faculdade de Odontologia de Piracicaba - Universidade Estadual de Campinas (UNICAMP)
Autores: Mamani MH, Araujo DS, Marquezin MCS, Barbosa TS, Gavião MBD, Castelo PM
Auxílio FAPESP 2012/04492-2 e 2011/15621-5
Evaluation of the relationship between the bite force and body mass index in young children: a preliminary study
Objective: Little is known about the influence of masticatory function on nutritional status in children, but it is assumed to be of importance. Thus, the aim of this preliminary study was to evaluate the relationship between maximum bite force (BF) and body mass index (BMI) in 39 children aged 7-8 years (7.42 y ± 0.49), in the early mixed dentition . BF was measured using a gnathodynamometer digital fork 10mm thick, in the left and right sides. Occlusion was evaluated using the Index of Orthodontic Treatment Need (IOTN). Data were analyzed using descriptive statistics, Komolgorov-Smirnov test and multiple linear regression to determine the relationship between BMI and the studied independent variables: age , gender , IOTN scores and right and left BF. The mean right BF was 291.65 N (±99.62) and mean left BF was 295.26 N (±109.01), with no significant difference between them. There was no significant relationship between BMI and the variables under study. It was concluded that there was no relationship between maximum BF and body mass index in the studied sample, and future studies in a larger number of subjects are needed to determine the influence of masticatory function on nutritional status in children.
ODTP-P12 AVALIAÇÃO DA RUGOSIDADE DE SUPERFÍCIE DE MATERIAIS RESINOSOS APÓS POLIMENTO E DEGRADAÇÃO QUÍMICA
Faculdade de Odontologia de Piracicaba - FOP/UNICAMP
Autores: Inagaki LT*, Cardoso M, Naufel FS, de Paula AB, Sobrinho LC, Puppin-RontaniR
Roughness evaluation of resin-based materials after polishing and chemical degradation
Objective: This study evaluated the influence of polishing systems and chemical degradation on surface roughness (SR) of composites. 72 samples (7 mm diameter x 2 mm thick) were prepared and randomly divided into 8 groups (n=9) in accordance with polishers Soflex (3M/ESPE) and Jiffy (Ultradent); composites (Filtek Z350XT and Vitalescense); and degradation solution (coffee (CF) and artificial saliva (AS)-control). After 24h at 37°C, samples were polished and initial SR was measured (Ra). Then, each sample was immersed in 4 mL of staining solution (CF: for 15 min, three times a day for 14 days). After that, final SR was measured and obtained ΔRa values. Data normality was verified by Kolmogorov-Smirnov test (p>0.05) and submitted to tree way ANOVA and Tukey test (5%). There were significant interaction between composites and polishers (p<0.001) and CF and AS showed no effect on RS. Independent of solutions, Vitalescence showed no significant change in RS when polished with Soflex (ΔRa=-0.05) and Jiffy (ΔRa=-0.09). However, Z350 showed greater variation in RS for Jiffy (ΔRa=-0.25) compared with SofLex (ΔRa=-0.01). After degradation, SofLex did not promote variations in SR for composites. However, Jiffy promoted smaller variations in SR for Vitlescence (ΔRa=-0.09) than Z350 (ΔRa=-0.25). In conclusion, the solutions did not affect the SR of composites. After degradation, Z350 polished with Jiffy showed decrease in SR, and composites polished with SofLex showed SR maintenance.
ODTP-P13 AVALIAÇÃO DA TEMPERATURA TRANSMITIDA À CÂMARA PULPAR DE DENTES RESTAURADOS COM CIMENTO DE IONÔMERO DE VIDRO E SUBMETIDOS ÀS ONDAS DE ULTRASSOM
Faculdade de Odontologia de Araraquara - UNESP
Autores: Zuanon ACC*, Mata M, Santos-Pinto LAM, Lins EC
Evaluation of the Pulp Chamber Temperature in Glass-Ionomer-Restored Teeth Submitted to Ultrasound Waves
Objective: The objective of the present study was to assess variations in the pulp chamber temperature of 48 human healthy premolars, which were divided into four experimental groups according to the glass-ionomer cement being used: Ketac Molar control (KC), Ketac Molar with ultrasound (KU), Vitremer control (VC) and Vitremer with ultrasound (VU). Methodology: After sectioning the apices and removing the root and coronal pulp, an orifice of 1.5 mm diameter x 2 mm depth was made in the buccal face of the teeth for insertion of the cements and later application of ultrasound. After filling the space left in coronal and root pulp with a thermo-conductor paste, a thermistor was inserted through the root canal to the pulp chamber for temperature rise. Ultrasound was applied to the cements for 45 seconds and photopolymerisation, if needed, for 40 seconds. Data were found to be normal, and linear regression tests, Pearson’s correlation, ANOVA and Student t-test were used for analysis. Results: The results showed that application of ultrasound to conventional and resin-modified glass Ionomer cements for 45 seconds increases the pulp chamber temperature by 0.07oC/s and 0.06oC/s, respectively, at a 95% confidence interval. Conclusion: One can conclude that the increased temperature found in the pulp chamber as a result of ultrasound application is safe for clinical use.
ODTP-P14 AVALIAÇÃO DAS FUNÇÕES ORAIS DE CRIANÇAS COM ASMA POR MEIO DO NORDIC OROFACIAL TEST-SCREENING
Faculdade de Odontologia de Piracicaba - UNICAMP
Autores: Amato JN*, Barbosa TS, Gavião MBD
Evaluation of oral functions of children with asthma using the Nordic Orofacial Test-Screening
Objective: This study aimed to evaluate the oral functions of children with asthma using the NordicOrofacial Test- Screening ( NOT- S ) protocol.Methods: Forty children with asthma (19 ♂, 21♀) and forty-two without asthma (19 ♂, 23♀), aged 7-9 yr-old, were selected from specialized clinic and public schools of Piracicaba/SP, respectively. The Brazilian Portuguese version of NOT-S was applied by a trained examiner by interview and clinical evaluation. The signs and symptoms of sleep bruxism and malocclusion (Index of Orthodontic Treatment Need) were also evaluated. Descriptive statistics, bivariate and multivariate analysis were used for data analysis, considering the level of significance of α = 0.05.Results: The NOT-S total scores ranged from 0 to 8; scores 3 and 2 were the most frequent observed in groups with (30.0%) and without asthma (33.3%). Group with asthma presented higher NOT-S total and NOT-S interview scores than those without asthma (p<0.05). Asthmatic children were approximately three times more likely to report impaired sensory function than children without asthma (p<0.05).Conclusion: The presence of asthma was associated with reports of impairment of sensory functions and presence of oral habits, as well as with clinical signs of respiratory dysfunction.
ODTP-P15 AVALIAÇÃO DAS PROPRIEDADES FÍSICO-QUÍMICAS DE MATERIAIS OBTURADORES UTILIZADOS EM DENTES DECÍDUOS
Universidade Estadual de Campinas/FOP-UNICAMP
Autores: Joaquim NM*, Varanda T, Iwamoto AS, Zeferino PV, Puppin-Rontani RM, PasconFM
Evaluation of physico-chemical properties of filling materials used in primary teeth
Objective: The aim of the study was to evaluate the OH- release and flow of filling materials used in primary teeth. For the first analysis, 60 bovine primary teeth were endodontically prepared and filled according to the groups (n=10): Control (without material) (C), Zinc Oxide Eugenol (ZOE), CalenÒ (CA), CA+OZ (CAOZ), CA+Iodofórmio (CAI), VitapexÒ (V). Teeth were immersed in deionized water and the pH was measured at 1 hour, 1, 7, 15 and 30 days. For flow analysis was used 0.05 ml of the materials placed on glass plates and pressed by another plate associated with 100g weight (ISO 6786, ANSI/ADA, nº57). The diameters were measured with a digital caliper. Data were analyzed by factorial ANOVA, ANOVA and Tukey tests (α=0.05). It was observed that OH- release in 1 hour showed no significant difference among groups (p>0.05). In one day, CA released more OH- and remained constant for 7, 15 and 30 days (p>0.05). The OH- release of CAOZ remained constant for all times and lower than all other materials. V released more OH- in 30 days. For the flow analysis, it was observed significant difference among materials studied (p<0.0001). Higher flow rate was observed for CA (21.28 ± 0.83), V (13.13 ± 0.19), CAI (10.57 ± 0.56), OZE (9.06 ± 0.49) and CAOZ (6.97 ± .26). It was concluded that among the materials studied, CalenÒ showed the greatest OH- release and higher flow ability compared to other materials.
ODTP-P16 AVALIAÇÃO DO BRILHO DE COMPÓSITOS SUBMETIDOS A DIFERENTES SISTEMAS DE POLIMENTO APÓS DEGRADAÇÃO QUÍMICA
Faculdade de Odontologia de Piracicaba - Unicamp
Autores: Cardoso M*, Inagaki LT, Naufel FS, de Paula AB, Correr-Sobrinho L, Puppin-Rontani RM.
Evaluation of brightness composites submitted to different polishing systems after chemical degradation
Objective: To evaluate the influence of staining in the glow of composites after polishing. Methodology: Seventy-two cylindrical samples (7x2mm) were made from Filtek Z350XT and Vitlescense composites. After 24h at 37 ° C, there were polished with Soflex systems (3M) and Jiffy (Ultradent) and randomly assigned into 8 groups (n = 9) according to the composite, polishing and staining solution [Coffee (CF) and saliva artificial (SA)]. Initial brightness measurements were performed. Then, each sample was immersed in 4 mL of CF for 15 min, 3x daily for 14 days. After, the brightness was measured again, allowing the calculation of variation (Δbrightness). The data were submitted to Kolmogorov-Smirnov tests, ANOVA factors 3 and Holmom-Sidak test (p<0,05). Results: There was significant interaction between restorative material and polishing system (p=0.007). Regardless of the solutions presented no significant ΔbrightnessVitlescence when polished with SL (3.80) and Jiffy (4.68). However, for Δbrightness Z350 was higher with SL (10.32) than with Jiffy (5.85). After degradation, regardless of polishers, Vitlescence showed no significant Δbrightness in CF and SA; already Z350 Δbrightness in SA showed lower (6.14) than in CF (10.03). Conclusion: After degradation in CF, Z350 showed higher Δbrightness when polished by SL; Vitlescence hasn’t presented Δbrightness. Therefore, in this study the brightness of the composite was dependent on the solution, the material and the polishing system.
ODTP-P17 AVALIAÇÃO IN VITRO DA LIBERAÇÃO DE ÍONS FLUORETO DE VERNIZES EM DIFERENTES SOLUÇÕES
Faculdade de Odontologia de Piracicaba - FOP/Unicamp
Autores: Varanda T*, Joaquim NM, Puppin-Rontani RM, PasconFM
In vitro fluoride release analysis of fluoride varnishes in different solutions
Objective: The aim of this study was to analyze the fluoride ions (F) release in vitro of 3 fluoride varnishes submitted to demineralization (DES) and remineralization (RE) solutions, as a function of time. For this, it was used polyester strips that received a standardized thin layer of varnishes. The procedure was performed by a trained and calibrated researcher. The specimens were randomly divided into three groups (n=5): Va (Vanish™-NaF varnish with 5% Tri-Calcium Phosphate), DR (Duraflur -varnish 5% NaF) and DP (Duraphat®-varnish 5% NaF) and 2 solutions: DES and RE. The specimens were individually immersed in 10 mL of each solution for 1, 3 , 7, 24 , 48, 72 and 120 hours, and changed in pre-established times. The determination of F release in the solutions was performed in duplicate, using a potentiometer (Orion Expandable Ion Analyzer 940 EA), which was expressed in mV and converted into ppm F. Data were submitted to ANOVA factorial and Tukey tests (α=0.05). It was not observed significant interaction among the studied factors: material, time and solutions (p>0.05). VA (0.693) released more F when compared to DR (0.624) and DP (0.565) (p<0.05). The highest concentration of F was observed in 72 (0.955) hours, with a significant difference for all times (p<0.05). Regarding solutions, the highest fluoride release it was observed for DES compared to RE. It could be concluded that the fluoride varnishes showed different fluoride release patterns, with highest release in 72 hours and for demineralization solution.
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