Supplemental text

E. coli type 1 pilus
fimA	no	HD0281 38% aa identity	no
fimB	no	no	no
fimH	no	no	no
fimC	no	HD0284 32% aa identity	no
fimD	no	HD0283 28% aa identity	no

C. Adheins and outer membrane proteins

Gene	H. somnus 129Pt	H. ducreyi 35000HP	H. influenzae Rd
H. somnus autotransporters
possible large adhesin	HS_0209	no	no
possible large adhesin	HS_0383	no	no
possible large adhesin	HS_0478	no	no
possible large adhesin	HS_0589	no	no
possible large adhesin	HS_0602	no	no
possible large adhesin	HS_0790	no	no
possible large adhesin	HS_1058	no	no
possible large adhesin	HS_1085	no	no
possible large adhesin	HS_1154	no	no
possible large adhesin	HS_1234	no	no
Autotransporter adhesin	HS_1543	no	no
possible large adhesin	HS_1616	no	no
possible large adhesin	HS_1632	no	HI1718
periplasmic zinc transporter/adhesin B precursor	HS_1256	HD0198	HI0119
	no	HD0281 major pilin protein precursor/adhesin B precursor	no
H. influenzae adhesins
hmw1A HMW1	no	no	no
hmw2A HMW2	no	no	no
hia	no	no	no
hsf	no	no	no
hap	no	no	no
H. ducreyi autotransporters
dsrA	HS_1543	HD0769	no
ncaA	no	HD1920	no
Outer membrane proteins
oapA	HS_1287	HD0651	HI0330
oapB	no	HD0652	HI0331
P2	no	HD1433 HD1435	HI0139
P1	HS_1548	HD1078	HI0401
P4	no	HD1170	HI0693
P5	HS_1035	HD0045 HD0046	HI1164
P6	HS_0263	HD1772	HI0381

Table S12. Genes for iron transport and utilization

Gene	H. somnus 129Pt	H. ducreyi 35000HP	H. influenzae Rd
TonB system
tonB	HS_0689	HD0327	HI0251
exbD	HS_0690	HD0328	HI0252
exbB	HS_0691	HD0329	HI0253
Fe-S cluster metabolism
cyaY	HS_1712 N-terminal	HD0473	HI0727.1 N-terminal
Fur
fur ferric uptake regulation protein	HS_1211	HD0367	HI0190
Heme biosynthesis
gltX glutamyl-tRNA synthetase	HS_0456	HD0320	HI0274
hemA glutamyl-tRNA reductase	HS_0810	no	no
hemB 5-aminolevulinate dehydratase (porphobilinogen synthase)	HS_0548	no	no
hemC hydroxymethylbilane synthase (porphobilinogen deaminase)	HS_0044	no	no
hemD uroporphyrinogen-III synthase	HS_0045	HD1742	no
hemE uroporphyrinogen decarboxylase	HS_1540	no	no
hemF coproporphyrinogen III oxidase	no	no	no
hemG protoporphyrin flavoprotein oxidase	HS_1569	no	no
hemH ferrochelatase	HS_1047	no	HI1160
hemL glutamate-1-semialdehyde aminotransferase	HS_1229	no	no
hemM (lolB) outer membrane lipoprotein LolB precursor	HS_0998	HD1629	HI1607
hemN coproporphyrinogen oxidase, oxygen-independent (coproporphyrinogen-III oxidase)	HS_0472	HD1668 HD0037 this one may be a paralog	HI0463
hemR hemin receptor, outer membrane protein	HS_0728	HD0388	HI0113
hemX uroporphyrin-III C-methyltransferase	HS_0046	HD1741	HI0603
hemY porphyrin biosynthesis protein	HS_0047	HD1740	HI0602
cyoE protohaeme IX farnesyltransferase	no	no	no
cysG siroheme synthase	no	no	no
aroB 3-dehydroquinate synthase	HS_1114	HD0422	HI0208
Transferrin binding proteins
tbpA transferrin-binding protein A	HS_0097* C-terminal region	no	HI0994
	HS_0098* central fragment	no
	HS_0102* N-terminal region	no
tbpB  transferrin-binding protein B	HS_0448	no	HI0995
tbpA   transferrin-binding protein A	HS_0449	no	HI0994
tbpA transferrin binding protein A	HS_0582	no	HI0994
transferrin-binding protein, putative	no	no	HI1217
Iron-regulated proteins
iron-regulated outer membrane protein	HS_0069	no	no
iron-regulated outer membrane protein	HS_1306	no	no
Heme utilization proteins
heme utilization protein hugZ	HS_0722	HD0620	no
heme utilization protein	HS_0723	no	no
tdhA TonB-dependent heme receptor A	no	HD0388	no
hxuC heme-hemopexin utilization protein C	no	no	HI0262
hxuB heme-hemopexin utilization protein B	no	no	HI0263
hxuA heme-hemopexin utilization protein A	no	no	HI0264
Hemoglobin binding proteins
hgbB	no	no	HI0635 20 CAAC repeats
hgbA	HS_0721 N-terminal HS_0720 C-terminal no repeats	HD2025 no repeats	HI0661 20 CAAC repeats near beginning of gene
hgbB	no	no	HI0712 36 CAAC repeats near beginning of gene
hemoglobin binding protein	no	no	HI1565 18 CAAC repeats near beginning of gene
TonB-dependent receptor, putative	no	no	HI1567
Iron transporters	15 total	9 total	17 total
Chelated iron ABC
yfeD	HS_0325	HD1024	HI0359
yfeC	HS_0326	HD1025	HI0360
yfeB	HS_0327	HD1817	HI0361
yfeA	HS_0328	HD1816	HI0362
Heme ABC
ccmA	HS_0393	HD0786	HI1089
ccmB	HS_0394	HD0787	HI1090
ccmC	HS_0395	HD0789	HI1091
ccmD	HS_0396	HD0790	HI1092
		HD0215	HI0853
Fe3+ /Iron III ABC
afuC	HS_0639	no	HI0126
afuB	HS_0640	no	HI0129
afuA	HS_0641	no	HI0131
afuA	HS_0643	no	no