Supplementary Online Material

Supplementary Online Material:

“Impact of sustaining a controlled residual growth on polyhydroxybutyrate yield and production kinetics in Cupriavidus necator”

Estelle Grousseau ^{a, b, c, d, *}, Elise Blanchet ^{a, b, c, d}, Stéphane Déléris ^d, Maria G. E. Albuquerque ^d, Etienne Paul ^{a, b, c}, Jean-Louis Uribelarrea<sup>a, b, c

a</sup> Université de Toulouse; INSA, UPS, INP; LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France

^b INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France

^c CNRS, UMR5504, F-31400 Toulouse, France

^d VEOLIA Environnement, Centre de Recherche sur l’Eau, Chemin de la Digue, BP 76, F-78603 Maisons-Laffitte cedex, France

The supporting information contains twelve pages, including metabolic network in C. necator with butyric acid as carbon source (S1), the anabolic network (S2), the catabolic network (S3), the list of metabolites (S4) of the metabolic descriptor; the feeding strategy with flow calculations (and indicators to readjust the feed to the biological requirement) (S5), the schematic flux distribution during different growth conditions (S6), and the references.

Abbreviations

ED, Entner-Doudoroff Pathway; G6PD, GXS, glyoxylate shunt; MFA, metabolic flux analysis; PCK, PEP carboxykinase; PEP, Phospho-Enol-Pyruvate; PHA, polyhydroxyalkanoate; PHB, polyhydroxybutyrate, PHV, polyhydroxyvalerate; PPP, Pentoses-phosphates pathway; S, Substrate; TCA, tricarboxylic acid cycle; THF, tetrahydrofolate; Triose-P, triose-phosphate = glyceraldehyde-3-phosphate or dihydroxyacetone-phosphate; VFA, volatile fatty acid; Xr, Residual Biomass.