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Supplementary figure for the materials and methods section
Fig. S1. Examples of data matrices corresponding to the modelling of branching scenarios. 1a, Data matrix corresponding to the dextran model. 1b, Representation of isomaltopentaose branching for the simulated scenario T. 1c, Representation of isomaltopentaose branching for the simulated scenario A (see Table 1).
Fig. S2. 1H NMR spectra of anomeric regions of gluco-oligosaccharides 1, 2 and 3 (see Fig. 2).
The NMR peaks are designated by the proton position on the residue and followed by the letter of the D-Glcp unit. For example, H1B -(1→4) indicates that the anomeric proton of D-Glcp unit B has the -configuration and is linked to the C4 of D-Glcp unit A.
Fig. S3. HMBC spectrum of tetrasaccharide 1 recorded in deuterium oxide at 298 K.
The code H-1/C-6 indicates a long-range coupling between C-6 of a D-Glcp unit and H-1 of a D-Glcp unit.
The code H-1/C-2, C-4/C-6 indicates a long-range coupling between C-2, C-4 or C-6 of a D-Glcp unit and H-1 of another D-Glcp unit.
Fig. S7. LC-MS analyses of the products obtained after hydrolysis by Aspergillus niger amyloglucosidase of -(1→2) glucosylated pentasaccharide 4 (-D-Glcp-(1→6)--D-Glcp-(1→6)--D-Glcp-(1→6)--D-Glcp-(1→4)-D-Glcp).
Numerals refer to the structures drawn in Fig. 4. Time value over each chromatogram indicates the reaction time. (♦), (*) before and after the Aspergillus nigeramyloglucosidase hydrolysis, respectively.
Fig. S8. 1H NMR spectra of anomeric regions of gluco-oligosaccharides 4, 6a or 6b and 7.
The NMR peaks are designated by the proton position on the D-Glcp unit and followed by the letter of the unit. For example, H1B -(1→4) indicates that the anomeric proton of D-Glcp unit B has the -configuration and is linked to the C4 of unit A.
Fig. S9. Percentage of glucose released from -(1→2) branched dextrans after the action of Aspergillus niger amyloglucosidase. The hydrolysis ratio is expressed as the molar ratio of the released D-Glcp units versus the D-Glcp units contained in the branched dextrans.