Table of Contens

Prerequisites: Foundation of Chemistry, Biology and Biochemistry

Evaluation: Written examination

Total number of hours: 22.5h (3 ECTS)

3. 
   B203: Biosensors and DNA biochips
   

Main professor: Dr. Bruno Le Pioufle (ENS Cachan)

Dr. Bruno Le Pioufle

http://www.ens-cachan.fr/le-pioufle-bruno-4464.kjsp

Objectives: The aim of this course is to make an overview of the biosensors technology, and more particularly on the DNA microarray, peptide and protein–soluble or not soluble- analysis chips, and cell analysis biochips. The course will permit to deepen some of the main physical or biophysical principles used for the fabrication and use of these biosensors.

Outline (with number of hours per part)	Lect	PSS	LW
Fabrication principle of DNA microarrays Soluble protein or peptide biochips --------2D electrophoresis --------Mass spectrometer coupling --------peptide microarrays – surface functionnalization aspects Membrane protein biochips --------Planar patch clamp technology --------miniaturization and noise Cell biochips --------Cell sorting using DEP, or microfluidic principles --------Cell detection and analysis using impedancemetry --------Ampérometric detection on a chip – advantage of miniaturized electrodes	2 1 1 1 2 2 2 2 2	2 2	4