| Detection Of Immun Response To Invivo Argulus Foliaceus L. Infestation In Carp (Cyprinus Carpio L.) By Elisa Technique
The carp, that have been cultivated since 1970s in Turkey, is the second important fish species reared in freshwaters of Turkey after rainbow trout. Argulus foliaceus, that is an Crustacean ectoparasite and called Carp loose, causes heavy infections in carp fish, particularly in juvenil fish, and economic loses in carp aquaculture.
In this study, 120 juvenil carp weighing 10-12 g were obtained from Istanbul University Sapaca Freshwater Fish Production Research and Applied Unit and placed in fiberglas tanks in Experimental Fish Diseases Laboratory of İstanbul University, Faculty of Aqutic Sciences. Fish were either infected with Argulus foliaceus by immersion or vaccinated with parasite exctract by intraperitonal injection , during two months. Experiments about rabbit immünizasyon were realized in DETAE, and results got from rabbit serum were compared with immünised fish serum’s.
In fish, vaccinated by immersion, immune response was not observed. However, it was observed in fish that were vaccinated by intraperitonal injection. Amount of antibodies the humoral response occuring in immunized fish and rabbit was determined by ELISA. Antigen extract with emulsifield adjuvant, was higher than without adjuvant. Finally, highest antibodies had been obtained immunized fish serum’s during 8 weeks. Response of immunized rabbit was also obtained higher than fish.
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