| Apoptotic And Antiproliferative Effects Of Paclitaxel İn Tissue Culture
In this thesis working, anti-proliferative and apoptotic effects of Paclitaxel, which is itself an anti-chemotherapeutic agent, to FM3A cell line originated from mouse mammary carcinoma at 7 different doses is researched. For this purpose, 7 different doses of Paclitaxel (P1 = 3 nM, P2 = 7.5 nM, P3 = 15 nM, P4 = 30 nM, P5 = 60 nM, P6 = 120 nM, P7 = 240 nM) is experimented to cells for 24 and 48 hours. Growth rate measurements, which are gained according to hemositometer method, showed that living cell number is decreased and number of dead cells is increased at statistically meaningful rates (p<0.05). Acquired growing rates are supported by mitochondrial dehydrogenises enzyme activity method.
Loss of volume, protrusions at plasma membrane (bleb formations), nuclear condensations and fragmentations, and apoptotic body formations are observed at cells which morphologic criteria examined by phase contrast, phase contrast with Giemsa and fluorescent microscope. According to apoptosis index rates determined by DAPI, most intense apoptotic cell formation is observed for P2 dose. Furthermore, at this dose, which is accepted as critical for this cell line, DNA fragmentation is showed by agaroz gel electrophoresis method.
Acquired deductions showed that between 7 different doses of Paclitaxel, P2 (7.5 nM) is the best dose to induce apoptosis in FM3A cells for 24 and 48 hours.
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