The sequence is given according to Genebank: af039855, U20477-88, U20510
Yüklə 123,76 Kb.
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| last update: 18.02.2007 RPE65 The sequence is given according to Genebank: AF039855, U20477-88, U20510. Exons are taken from (16) and sequences were completed with additional data obtained in the editor’s lab Primers are given according to (2) and (15) Nucleotide numbering Aminoacid numbering Sequence variations (Polymorphism, rare variant, LCA2, ARRP, LCA2 or ARRP) Mutations detectable by a mutation micro array are given in italics, and shown in grey if no reference can be allocated to these -170 -160 -150 -140 -130 -120 -110 |-----------------------> E1-f (2) |---------------------------> RPE65-1f (15) gaga gctga aagca acttc tgttc cccct ccctc agctg aaggg gtggg gaagg gcctc ccaaa gccat aactc ctct cgact ttcgt tgaag acaag gggga gggag tcgac ttccc caccc cttcc cggag ggttt cggta ttgag
-100 -90 -80 -70 -60 | -50 -40 -30 ctttt aaggg attta gaagg cataa aaagg cccct ggctg agaac ttcct tcttc attct gcagt tggtg ccaga gaaaa ttccc taaat cttcc gtatt tttcc gggga ccgac tcttg aagga agaag taaga cgtca accac ggtct C=Thr(1,14) a (2,3,13,7,17,23,24) (7) a -20 -10 1| 11 | | actct ggatc ctgaa ctgga agaaa ATG TCT ATC CA gtaag tatct ctggg agact ttttt aaaac acctt tgaga cctag gactt gacct tcttt TAC AGA TAG GT cattc ataga gaccc tctga aaaaa ttttg tggaa M S I Q Met Ser Ile Gln 1 2 3 4
gtacc taagt actat ttaca cgata acatt cccga ggact tctct t <------ag------------------| RPE65-1r (15) <---------------------------| E1-R (2) IVS 1 |-----------------------> E2-F (2) |---------------------------> RPE65-2f (15) t ctatc tctgc ggact ttgag catca acatg ggctt cttcc ttatt cttcc accat ttcag a gatag agacg cctga aactc gtagt tgtac ccgaa gaagg aataa gaagg tggta aagtc del (5) C=Phe (1) | C=Pro (6,25) 12 15 30 45 | |-|60 | 75 G GTT GAG CAT CCT GCT GGT GGT TAC AAG AAA CTG TTT GAA ACT GTG GAG GAA CTG TCC TCG CCG C CAA CTC GTA GGA CGA CCA CCA ATG TTC TTT GAC AAA CTT TGA CAC CTC CTT GAC AGG AGC GGC Q V E H P A G G Y K K L F E Thr V E E L S S P Gln Val Glu His Pro Ala Gly Gly Tyr Lys Lys Leu Phe Glu Thr Val Glu Glu Leu Ser Ser Pro 4 5 10 15 20 25 insT (2,14) | 94
CTC ACA GCT CAT GTA ACA G gttgg tctcg cccat cttga agcca tcctc tttta tgtca gttcc tctct GAG TGT CGA GTA CAT TGT C caacc agagc gggta gaact tcggt aggag aaaat acagt caagg agaga L T A H V T G <------ Leu Thr Ala His Val Thr Gly <-x-------- 30 32
agaga ccgaa ggata aggaa ccgca gagag tctcg tctga ggggg acacc cagtg tccct ctccc tagtc cacca ----------------| RPE65-2r (15) --------| E2-R (2) ttggg ctttt tatag caaga accca ttttc tttca ttcgc cttta cttta aaccc gaaaa atatc gttct tgggt aaaag aaagt aagcg gaaat gaaat IVS 2 agggc tggaa atgaa aatca ctgcc agctc tatga ggaag aagct gccca atcag gctgc tgata tcccg acctt tactt ttagt gacgg tcgag atact ccttc ttaga cgggt tagtc agacg actat |----------------------> E3-F (2) (22) t |---------------------------> RPE65-3f (15) | tactc tgcct tacca aggac aagcc tagcc caagg caggg ataag aagca atgtt ctgtc tcccc ttcat cacag atgag acgga atggt tcctg ttcgg atcgg gttcc gtccc tattc ttcgt tacaa gacag agggg aagta gtgtc del (27) A=Ser (1,17,23,24) T=Val (27) |---------| | (17) A=Gln delG (13,23,24) | 105 | | | | 135 | 150 GC AGG ATC CCC CTC TGG CTC ACC GGC AGT CTC CTT CGA TGT GGG CCA GGA CTC TTT GAA GTT GGA CG TCC TAG GGG GAG ACC GAG TGG CCG TCA GAG GAA GCT ACA CCC GGT CCT GAG AAA CTT CAA CCT G R I P L W L T G S L L R C G P G L F E V G Gly Arg Ile Pro Leu Trp Leu Thr Gly Ser Leu Leu Arg Cys Gly Pro Gly Leu Phe Glu Val Gly 32 35 40 45 50
165 180 | 195 |-| | 225 TCT GAG CCA TTT TAC CAC CTG TTT GAT GGG CAA GCC CTC CTG CAC AAG TTT GAC TTT AAA GAA GGA AGA CTC GGT AAA ATG GTG GAC AAA CTA CCC GTT CGG GAG GAC GTG TTC AAA CTG AAA TTT CTT CCT S E P F Y H L F D G Q A L L H K F D F K E G Ser Glu Pro Phe Tyr His Leu Phe Asp Gly Gln Ala Leu Leu His Lys Phe Asp Phe Lys Glu Gly 55 60 65 70 75
| C=His (14) | | 240 245 CAT GTC ACA TAC CAC AGA AG gtaaa gcagc actcc atgcc actcc tcctc ctcaa agtag ggcct agctt GTA CAG TGT ATG GTG TCT TC cattt cgtcg tgagg tacgg tgagg aggag gagtt tcatc ccgga tcgaa H V T Y H R R RPE65-3r (15) <---------------------------| His Val Thr Tyr His Arg Arg 80 82
ccgag gagga gggta cactc aaaag tggag acttg agtcc tccgt tggat atatg ggtga aagaa gagtg ttaca
tggac ccggt cacac agacg tacct atgct ccctt gtaat agtac IVS 3 taata aaacc cttta ttctt catgt tgtgc attta tgatt gtgac ttgat gagga cacat agaat attat tttgg gaaat aagaa gtaca acacg taaat actaa cactg aacta ctcct gtgta tctta |---------------------> E4-F (2) |---------------------------> RP4f (15) ggcca ttcta agctc cacat gggct gtacg gattg ctcct gtcta tactc ttccc tatgt ttcaa tgtcc ttcag ccggt aagat tcgag gtgta cccga catgc ctaac gagga cagat atgag aaggg ataca aagtt acagg aagtc T=Trp (1,14) |A=Gln (8,14,23,24,17) |G=Pro del20bp (13,14,18) |ins A |-----------------------| || | ter=T (19)
246 | 270 || | | 300 | | G TTC ATC CGC ACT GAT GCT TAC GTA CGG GCA ATG ACT GAG AAA AGG ATC GTC ATA ACA GAA TTT C AAG TAG GCG TGA CTA CGA ATG CAT GCC CGT TAC TGA CTC TTT TCC TAG CAG TAT TGT CTT AAA R F I R T D A Y V R A M T E K R I V I T E F Arg Phe Ile Arg Thr Asp Ala Tyr Val Arg Ala Met Thr Glu Lys Arg Ile Val Ile Thr Glu Phe 82 85 90 95 100
| 315 330 345 353 | GGC ACC TGT GCT TTC CCA GAT CCC TGC AAG AAT ATA TTT TCC AG gttac tgaac ccaaa ctgaa tgtta CCG TGG ACA CGA AAG GGT CTA GGG ACG TTC TTA TAT AAA AGG TC caatg acttg ggttt gactt acaat G T C A F P D P C K N I F S R Gly Thr Cys Ala Phe Pro Asp Pro Cys Lys Asn Ile Phe Ser Arg 105 110 115 118
gagtt ctgta aaata taatc gggaa aaaga gagta aaaat ataat cggga aaaag agag RPE65-4r <--------------------------|
|------------------------> RPE65-5f (15) 354 360 | 375 ggc ttgaa aatta ctgga ctgaa aaatt cattt gtttc tacag G TTT TTT TCT TAC TTT CGA GGA GTA ccg aactt ttaat gacct gactt tttaa gtaaa caaag atgtc C AAA AAA AGA ATG AAA GCT CCT CAT R F F S Y F R G V Arg Phe Phe Ser Tyr Phe Arg Gly Val 118 120 125
| C=Leu (1) | T=Tyr (1) | 390 | | 405 420 | | 435 | GAG GTT ACT GAC AAT GCC CTT GTT AAT GTC TAC CCA GTG GGG GAA GAT TAC TAC GCT TGC ACA GAG CTC CAA TGA CTG TTA CGG GAA CAA TTA CAG ATG GGT CAC CCC CTT CTA ATG ATG CGA ACG TGT CTC E V T D N A L V N V Y P V G E D Y Y A C T E Glu Val Thr Asp Asn Ala Leu Val Asn Val Tyr Pro Val Gly Glu Asp Tyr Tyr Ala Cys Thr Glu 130 135 140 145 ins g 450 465 480 495 | ACC AAC TTT ATT ACA AAG ATT AAT CCA GAG ACC TTG GAG ACA ATT AAG CAG gtggg acaca gtgct TGG TTG AAA TAA TGT TTC TAA TTA GGT CTC TGG AAC CTC TGT TAA TTC GTC caccc tgtgt cacga T N F I T K I N P E T L E T I K Q Thr Asn Phe Ile Thr Lys Ile Asn Pro Glu Thr Leu Glu Thr Ile Lys Gln 150 155 160 165
tccac tacaa gtcct taaat cttaa acctt gaatt ttaat taagt ggttg tattt aataa gtacg acgct tacat RPE65-5r (15) <-------------------------t-| <----------------
actaa gattg aacta ctcct gtgtg tctta ctggt aaggt tcgag gttta ---------| E5-R (2)
tctcc catct taggt ctctc taccc ttctt tctct tcacg tgaat cctac tctca agttc cccat cacta |----------------------------> R6-F (2) |---------------------------> RPE65-6f (15) gacct cagac ctagg gacaa aggta taatg tatct tcctt ctctc aactg gagga cattc acttt acttc cgtag ctgga gtctg gatcc ctgtt tccat attac ataga aggaa gagag ttgac ctcct gtaag tgaaa tgaag gcatc C=Asn (22) T=Tyr (14) T=Tyr (1,23,24,17) 498 | 510 525 540 | 555 GTT GAT CTT TGC AAC TAT GTC TCT GTC AAT GGG GCC ACT GCT CAC CCC CAC ATT GAA AAT GAT GGA CAA CTA GAA ACG TTG ATA CAG AGA CAG TTA CCC CGG TGA CGA GTG GGG GTG TAA CTT TTA CTA CCT V D L C N Y V S V N G A T A H P H I E N D G Val Asp Leu Cys Asn Tyr Val Ser Val Asn Gly Ala Thr Ala His Pro His Ile Glu Asn Asp Gly 166 170 175 180 185
| G=Ser (1) T=Tyr (17) | |del (14) | 585 600 | ||-| ACC GTT TAC AAT ATT GGT AAT TGC TTT GGA AAA AAT TTT TCA ATT GCC TAC AAC ATT GTA AAG ATC TGG CAA ATG TTA TAA CCA TTA ACG AAA CCT TTT TTA AAA AGT TAA CGG ATG TTG TAA CAT TTC TAG T V Y N I G N C F G K N F S I A Y N I V K I Thr Val Tyr Asn Ile Gly Asn Cys Phe Gly Lys Asn Phe Ser Ile Ala Tyr Asn Ile Val Lys Ile 190 195 200 205
| a (14) 630 643 | | CCA CCA CTG CAA GCA G gtgag tttac caatc tgtcc tcttc tgaaa ataag tgtct atatt gtgag aaagt GGT GGT GAC GTT CGT C cactc aaatg gttag acagg agaag acttt tattc acaga tataa cactc tttca P P L Q A D <- Pro Pro Leu Gln Ala Asp <------------ 210 215
atgac ataac actct ttttt attct ccgac aaggt ttcg... ---------------------c ----| RPE65-6r (15) ----------------| E6-R (2)
RPE65-7f |del (20) c (20) |t |---------------------------> || | || ctg ttcta aatgc tttgt attaa aaaaa aaata agagg ctgtt ccaat gcctt ttaaa accac tttat ttcag gac aagat ttacg aaaca taatt ttttt tttat tctcc gacaa ggttt cggaa aattt tggtg aaata aagtc
| 660 675 690 | 705 AC AAG GAA GAT CCA ATA AGC AAG TCA GAG ATC GTT GTA CAA TTC CCC TGC AGT GAC CGA TTC AAG TG TTC CTT CTA GGT TAT TCG TTC AGT CTC TAG CAA CAT GTT AAG GGG ACG TCA CTG GCT AAG TTC D K E D P I S K S E I V V Q F P C S D R F K Asp Lys Glu Asp Pro Ile Ser Lys Ser Glu Ile Val Val Gln Phe Pro Cys Ser Asp Arg Phe Lys 215 220 225 230 235
| 720 725 | CCA TCT TAC GTT CAT AG gtaac ttgaa ggcct gctat gaatc ttcag gaaaa ctcaa gttta aagat ttgct GGT AGA ATG CAA GTA TC cattg aactt ccgga cgata cttag aagtc ctttt gagtt caaat ttcta aacga P S Y V H S <------------------- Pro Ser Tyr Val His Ser 240 242
aacgg atgga --------| RPE65-7b
...aa ctatg gttag gacta gtgtt ataaa aaaac gtctt ttgat ttaaa tttgt attaa ttaaa gtaca tccgt RPE65-8a |--------------------------> ctgtt gattc ttgta actga gcata ccaca tatat acaaa tttgt ctgtg gcttg agaat cagcc ctttc attca gacaa ctaag aacat tgact cgtat ggtgt atata tgttt aaaca gacac cgaac tctta gtcgg gaaag taagt
caagc ccatt tgtgt ttctg aacag T TTT GGT CTG ACT CCC AAC TAT ATC GTT TTT GTG GAG ACA CCA gttcg ggtaa acaca aagac ttgtc A AAA CCA GAC TGA GGG TTG ATA TAG CAA AAA CAC CTC TGT GGT S F G L T P N Y I V F V E T P Ser Phe Gly Leu Thr Pro Asn Tyr Ile Val Phe Val Glu Thr Pro 242 245 250 255 del (2,14) |---------| 795 810 825 GTC AAA ATT AAC CTG TTC AAG TTC CTT TCT TCA TGG AGT CTT TGG GGA GCC AAC TAC ATG GAT TGT CAG TTT TAA TTG GAC AAG TTC AAG GAA AGA AGT ACC TCA GAA ACC CCT CGG TTG ATG TAC CTA ACA V K I N L F K F L S S W S L W G A N Y M D C Val Lys Ile Asn Leu Phe Lys Phe Leu Ser Ser Trp Ser Leu Trp Gly Ala Asn Tyr Met Asp Cys 260 265 270 275
840 855 858 | | TTT GAG TCC AAT GAA ACC ATG GGG gtaag tctta gatat atttg tcaag tttgt gattc tgaag aagat AAA CTC AGG TTA CTT TGG TAC CCC cattc agaat ctata taaac agttc aaaca ctaag acttc ttcta F E S N E T M G <---------- Phe Glu Ser Asn Glu Thr Met Gly 280 285 286
cacaa attat tacat gtg --------------------| RPE65-8b
gt acact ttttt ccttt ttaaa tgcat caaaa tattt tcctc atttt tcaag ca tgtga aaaaa ggaaa aattt acgta gtttt ataaa aggag taaaa agttc
| del (17) C=Phe (13) | | del (13) T=ter (8) | 870 | 885 | | 900 | 915 GTT TGG CTT CAT ATT GCT GAC AAA AAA AGG AAA AAG TAC CTC AAT AAT AAA TAC AGA ACT TCT CCT CAA ACC GAA GTA TAA CGA CTG TTT TTT TCC TTT TTC ATG GAG TTA TTA TTT ATG TCT TGA AGA GGA V W L H I A D K K R K K Y L N N K Y R T S P Val Trp Leu His Ile Ala Asp Lys Lys Arg Lys Lys Tyr Leu Asn Asn Lys Tyr Arg Thr Ser Pro 290 295 300 305
930 945 | 960 || 975 | | TTC AAC CTC TTC CAT CAC ATC AAC ACC TAT GAA GAC AAT GGG TTT CTG ATT GTG GAT CTC TGC TGC AAG TTG GAG AAG GTA GTG TAG TTG TGG ATA CTT CTG TTA CCC AAA GAC TAA CAC CTA GAG ACG ACG F N L F H H I N T Y E D N G F L I V D L C C Phe Asn Leu Phe His His Ile Asn Thr Tyr Glu Asp Asn Gly Phe Leu Ile Val Asp Leu Cys Cys 310 315 320 325 330
TGG AAA GG gtaag aaagg acact ggaca aatgg gacac ctccc attgt tcctg gaaat tacgg ggttt ttaca ACC TTT CC cattc tttcc tgtga cctgt ttacc ctgtg gaggg taaca aggac cttta atgcc ccaaa aatgt W K G <------------------------| RPE65-9b Trp Lys Gly 333
ctcca aaaat gtctc gacga cgtga gttag actta gtgta gattt tgtcg ttctc aatac actct cagtt tttac
attaa ccttt tcgtc atcct tcaaa tccat tagac ttgta gtttt gatac taatt actaa ctttt gatta gccca ccacg aaccc cacct gaaaa caaaa gattc tgtga cctga gaggt gaact atttc aactg tttt ggtgc ttggg gtgga ctttt gtttt ctaag acact ggact ctcca cttga taaag ttgac aaaa IVS 9|-----------------------> RPE65-10a agaat catct ctcta aaatt atttg tctta gtaga gagat tttaa taaac (1)Ser=C 999 1005 1020 | tcatt gcctg tgctc atgtt tgact tttta tttt tgcag A TTT GAG TTT GTT TAT AAT TAC TTA TAT TTA agtaa cggac acgag tacaa actga aaaat aaaa acgtc T AAA CTC AAA CAA ATA TTA ATG AAT ATA AAT G F E F V Y N Y L Y L Gly Phe Glu Phe Val Tyr Asn Tyr Leu Tyr Leu 333 335 340
| G=Glu (15) | | A=Glu (1,17) | | | del (3,14,15) | | | | ins1bp (13,23,24) | | | | | C=Pro | | | | | | A=Thr (2,14) 1035 |1050 | | | | | | 1095 GCC AAT TTA CGT GAG AAC TGG GAA GAG GTG AAA AAA AAT GCC AGA AAG GCT CCC CAA CCT GAA GTT CGG TTA AAT GCA CTC TTG ACC CTT CTC CAC TTT TTT TTA CGG TCT TTC CGA GGG GTT GGA CTT CAA A N L R E N W E E V K K N A R K A P Q P E V Ala Asn Leu Arg Glu Asn Trp Glu Glu Val Lys Lys Asn Ala Arg Lys Ala Pro Gln Pro Glu Val 345 350 355 360 365
|G=Cys del (11,25) || 1110 | 1128 AGG AGA TAT GTA CTT CCT TTG AAT ATT GAC AAG gtaac ctgct tctct gtaga tttca gattt aacca TCC TCT ATA CAT GAA GGA AAC TTA TAA CTG TTC cattg gacga agaga catct aaagt ctaaa ttggt R R Y V L P L N I D K <-- Arg Arg Tyr Val Leu Pro Leu Asn Ile Asp Lys 370 375 376 |---------------------------> RPE65-11a gaatg tttca tctct ctcag IVS10 gttt gaatt ctttc ctgct cactg aggtt tctgt tatct tctct cctag cttac aaagt agaga gagtc caaa cttaa gaaag gacga gtgac tccaa agaca ataga agaga ggatc ----------------------| RPE65-10b A=Thr (1,17) G=Gly (13,23,24) 1131 1140 | 1170 | 1185 GCT GAC ACA GGC AAG AAT TTA GTC ACG CTC CCC AAT ACA ACT GCC ACT GCA ATT CTG TGC AGT GAC CGA CTG TGT CCG TTC TTA AAT CAG TGC GAG GGG TTA TGT TGA CGG TGA CGT TAA GAC ACG TCA CTG A D T G K N L V T L P N T T A T A I L C S D Ala Asp Thr Gly Lys Asn Leu Val Thr Leu Pro Asn Thr Thr Ala Thr Ala Ile Leu Cys Ser Asp 377 380 385 390 395
1200 || | 1215 | | 1230 1243 GAG ACT ATC TGG CTG GAG CCT GAA GTT CTC TTT TCA GGG CCT CGT CAA G gtgag atgat ctaga gaaaa CTC TGA TAG ACC GAC CTC GGA CTT CAA GAG AAA AGT CCC GGA GCA GTT C cactc tacta gatct ctttt E T I W L E P E V L F S G P R Q A Glu Thr Ile Trp Leu Glu Pro Glu Val Leu Phe Ser Gly Pro Arg Gln Ala 400 405 410 415
cttca cacgg gagtg aacaa atgtt tcttt caaag agatt aaga gtttt cctaa gcatg tgctc tattt cgtag gaagt gtgcc ctcac ttgtt tacaa agaaa gtttc tctaa ttct caaaa ggatt cgtac acgag ataaa gcatc
| (27) Cys=G | | (1) Ala=C | |
1245 | 1260 1275 1290 | | | | | CA TTT GAG TTT CCT CAA ATC AAT TAC CAG AAG TAT TGT GGG AAA CCT TAC ACA TAT GCG TAT GGA GT AAA CTC AAA GGA GTT TAG TTA ATG GTC TTC ATA ACA CCC TTT GGA ATG TGT ATA CGC ATA CCT A F E F P Q I N Y Q K Y C G K P Y T Y A Y G Ala Phe Glu Phe Pro Gln Ile Asn Tyr Gln Lys Tyr Cys Gly Lys Pro Tyr Thr Tyr Ala Tyr Gly 415 420 425 430 435
1320 1338 | CTT GGC TTG AAT CAC TTT GTT CCA GAT AGG gtaat taatc cttct tacta atatt tgaac agtgc tttga GAA CCG AAC TTA GTG AAA CAA GGT CTA TCC catta attag gaaga atgat tataa acttg tcacg aaact L G L N H F V P D R Leu Gly Leu Asn His Phe Val Pro Asp Arg 440 445 446
gtata tgcta gtcaa gtaaa gcata ttgac tgatt gcttg attga ttttt ctttc tcaca aacag catat acgat cagtt cattt cgtat aactg actaa cgaac taact aaaaa gaaag agtgt ttgtc
| G=Gly (1) | T=ter (13) 1341 | | 1365 | 1380 | 1395 CTC TGT AAG CTG AAT GTC AAA ACT AAA GAA ACT TGG GTT TGG CAA GAG CCT GAT TCA TAC CCA TCA GAG ACA TTC GAC TTA CAG TTT TGA TTT CTT TGA ACC CAA ACC GTT CTC GGA CTA AGT ATG GGT AGT L C K L N V K T K E T W V W Q E P D S Y P S Leu Cys Lys Leu Asn Val Lys Thr Lys Glu Thr Trp Val Trp Gln Glu Pro Asp Ser Tyr Pro Ser 447 450 455 460 465 A=Asp (1,3,23,24) 1410 | 1425 1440 1450 GAA CCC ATC TTT GTT TCT CAC CCA GAT GCC TTG GAA GAA GAT GAT G gtaat gaaag caatt gttgt CTT GGG TAG AAA CAA AGA GTG GGT CTA CGG AAC CTT CTT CTA CTA C catta ctttc gttaa caaca E P I F V S H P D A L E E D D G Glu Pro Ile Phe Val Ser His Pro Asp Ala Leu Glu Glu Asp Asp Gly 470 475 480 484
cagac ttatg agaag aatga cgtca agaca tacaa tcaag tcatc ctttc ctcat tccaa ctaat aaggc attat <---------------------------| RPE65-13b ctcaa aaacg atcct taata ctata aatag agcag tactg aagaa ttaaa actat ttgaa gagtt tttgc tagga attat gatat ttatc tcgag atgac ttctt aattt tgata aactt IVS 13 tca gaaaa agaag tcagg tcata tggtt ttcta atttg tcaat gtaat acctc ctata agt ctttt tcttc agtcc agtat accaa aagat taaac agtta catta tggag gatat t (17) |---------------------------> RPE65-14 | ttatt tcaat gacat tcaat ctata gcttg ggctt ttaaa aactc aatat tgcct aattt acttc tgata aacag aataa agtta ctgta agtta gatat cgaac ccgaa aattt ttgag ttata acgga ttaaa tgaag actat ttgtc A=Asp | 1455 1470 1485 1500 1515 GT GTA GTT CTG AGT GTG GTG GTG AGC CCA GGA GCA GGA CAA AAG CCT GCT TAT CTC CTG ATT CTG CA CAT CAA GAC TCA CAC CAC CAC TCG GGT CCT CGT CCT GTT TTC GGA CGA ATA GAG GAC TAA GAC G V V L S V V V S P G A G Q K P A Y L L I L Gly Val Val Leu Ser Val Val Val Ser Pro Gly Ala Gly Gln Lys Pro Ala Tyr Leu Leu Ile Leu 485 490 495 500 505
1530 | | 1575 AAT GCC AAG GAC TTA AGT GAA GTT GCC CGG GCT GAA GTG GAG ATT AAC ATC CCT GTC ACC TTT CAT TTA CGG TTC CTG AAT TCA CTT CAA CGG GCC CGA CTT CAC CTC TAA TTG TAG GGA CAG TGG AAA GTA N A K D L S E V A R A E V E I N I P V T F H Asn Ala Lys Asp Leu Ser Glu Val Ala Arg Ala Glu Val Glu Ile Asn Ile Pro Val Thr Phe His 510 515 520 525
| | 1602 1622 1632 1642 1652 1662 GGA CTG TTC AAA AAA TCT TGA gcata ctcca gcaag atatg ttttt ggtag caaaa ctgag aaaat cagct CCT GAC AAG TTT TTT AGA ACT cgtat gaggt cgttc tatac aaaaa ccatc gtttt gactc tttta gtcga G L F K K S * <---------- Gly Leu Phe Lys Lys Ser * 530 533
tcagg tctgc aatca aattc tgttc aattt tagcc tgcta tatgt catgg tttta acttg cagat gcgca caatt agtcc agacg ttagt ttaag acaag ttaaa atcgg acgat ataca gtacc aaaat tgaac gtcta cgcgt gttaa ----------------| RPE65-14b 1742 1752 1762 1772 1782 1792 1802 1812 ttgca atgtt ttaca gaaag cactg agttg agcaa gcaat tcctt tattt aaaaa aaaaa gtacg tattt agata aacgt tacaa aatgt ctttc gtgac tcaac tcgtt cgtta aggaa ataaa ttttt ttttt catgc ataaa tctat
atcat acttc ctctg tgaga caggc cataa ctgaa aaact cttaa atatt tagca atcaa atagg aaatg aatgt tagta tgaag gagac actct gtccg gtatt gactt tttga gaatt tataa atcgt tagtt tatcc tttac ttaca
ggact tacta aatgg ctttt aattc ctatt ataag agcat atttt aggta cctat ctgct ccaat tatat tttta cctga atgat ttacc gaaaa ttaag gataa tattc tcgta taaaa tccat ggata gacga ggtta atata aaaat
acatt taaaa accaa agtcc tctac acttg attta tatta tatgt ggctt tgctg agtca aggaa gtatc atgca tgtaa atttt tggtt tcagg agatg tgaac taaat ataat ataca ccgaa acgac tcagt tcctt catag tacgt
ataag gctta attac taaat gtcaa accaa acttt ttctc aaacc aggga ctatc atcta agatt aatta cagta tattc cgaat taatg attta cagtt tggtt tgaaa aagag tttgg tccct gatag tagat tctaa ttaat gtcat
attat tttgc gtata cgtaa ctgct caaag attat gaatc ttatg aatgt taacc tttcc gttta ttaca agcaa taata aaacg catat gcatt gacga gtttc taata cttag aatac ttaca attgg aaagg caaat aatgt tcgtt
gtact attat ttctg atttt ataat aagaa aatct atgtt taatc aactg aggcc tctca accaa ataac atctc catga taata aagac taaaa tatta ttctt ttaga tacaa attag ttgac tccgg agagt tggtt tattg tagag
agaga ttaag ttata tatta aaagc ttatg taaca taaaa gcaag tacat atagt agtga ctata tttaa aaaaa tctct aattc aatat ataat tttcg aatac attgt atttt cgttc atgta tatca tcact gatat aaatt ttttt
cagag cataa aatgc ttaaa aatgt aatat ttact aaaat cagat tatgg gataa tgttg cagga ttata cttta gtctc gtatt ttacg aattt ttaca ttata aatga tttta gtcta atacc ctatt acaac gtcct aatat gaaat
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