Traditional Posters: Miscellaneous

Miriam Scadeng¹, Ellen Breen², Nathan Gray¹, David Dubowitz<sup>1

1</sup>Radiology, UC San Diego, San Diego, CA, United States; ²Medicine, UC San Diego, San Diego, CA, United States

Being a relatively new animal model there is no comprehensive 3D anatomical atlas onto which temporal or spatial data can be projected. In addition, methods for in-vivo imaging of adult fish are needed if zebrafish researchers are to benefit from functional MR imaging techniques such as MRS, MEMRI, BOLD and even ASL that are routinely being used in mouse models of disease. The major challenges include the very small size of the fish, and imaging the live fish in water. We present methods for in-vivo MRI of zebrafish, and a 3D atlas of zebrafish anatomy.

1046. Visualization of Vascular Casts Using 3D MR Imaging

Ian Rowland¹, Joseph Heintz², Douglas Steeber³, Ralph Albrecht<sup>2

1</sup>Department of Radiology, University of Wisconsin, Madison, WI, United States; ²Department of Animal Sciences, University of Wisconsin, Madison, WI, United States; ³Department of Biological Sciences, University of Wisconsin, Milwaukee, WI, United States

This study demonstrates that at 4.7T, using a standard 3D gradient echo sequence, images of vascular casts prepared using established corrosion casting techniques may be obtained with an isotropic resolution <50um. Immersion of the casts in aqueous contrast agent enabled 3D representations of the casts to be generated via the absence of MR signal. Despite the lower resolution of MR, surface rendering of the data is able to guide SEM studies to specific regions of interest. Accurate 3D data from vascular casts can be compared with in vivo angiography studies and may also be used to validate direct and indirect methods of measuring vascular dimensions.

1047. EPI Microscopy with Uniplanar Magnetic Field Gradient Coils

Lin Zhao¹, Andrey V. V. Demyanenko¹, J. Michael Tyszka<sup>1

1</sup>California Institute of Technology, Pasadena, CA, United States

A localized small gradient design generates negligible eddy currents and is well-suited for EPI microscopy. Here single shot EPI is demonstrated in both an ex vivo mouse embryo sample and in vivo mouse brain using a prototype three-axis uniplanar gradient design.

1048. MR-Microscopy on a Human 7T-Scanner

Andreas Berg¹, Andreas Potthast², Piotr Starewicz<sup>3

1</sup>MR-Center of Excellence, Center for Biomedical Engineering and Physics, Wien, Vienna, Austria; ²Healthcare Sector, Imaging & IT Division, Magnetic Resonance, Siemens AG, Erlangen, Germany; ³Resonance Research, Inc., Billerica, MA, United States

MR-microscopy can be looked upon as key technological tool in MRI-based molecular imaging in biological models and human pathology samples. Is it possible to achieve a pixel-size below 100 µm on high-field human MR-scanners (B≥7T) within 21min at acceptable Signal-to-Noise-Ratio? We present microscopic images at voxel-size of 25x51x200 µm³ and document a spatial resolution higher than 34µm using specifically designed grid-phantoms. The data, according to our knowledge, represents the highest documented spatial resolution obtained yet on such a scanner. The technical equipment based on a high gradient-strength insert coil and sensitive detectors is shortly explained.

1049. An In-Ovo Longitudinal µ-Magnetic Resonance Imaging Study of Quail Eggs and Embryonic Development

Suzanne Louise Duce<sup>1

1</sup>College of Life Sciences, University of Dundee, Dundee, United Kingdom

Avian embryos are important models for understanding embryonic development. We undertook a 3D longitudinal μMRI study using 7.1T Bruker of quail eggs imaged daily for 8 days, changes in the extra- and non-embryonic components as well as in the embryo were studied. At Day 0 the yolk, albumen, latebra and chalaza are visible, additional aqueous region is visible from Day 2. The growth of the embryo in-ovo is visualised. The 3D surface reconstructions of the embryo, yolk, albumen, embryonic fluid, and latebra were produced, their respective volumes calculated and changes over time presented graphically.