Traditional Posters: Miscellaneous

Frank Risse¹, Jelena Pesic¹, Simon Young², Lars E. Olsson<sup>1

1</sup>DECS Imaging & Antibodies, AstraZeneca R&D, Mölndal, Sweden; ²Bioscience, AstraZeneca R&D, Charnwood, United Kingdom

The effect of steroid (budesonide) treatment on lung inflammation in rats caused by ovalbumin can be visualised using hyperpolarised ³He MRI. The aim was to quantify the changes in lung ventilation in this asthma model using texture analysis. Four groups were investigated: controls, vehicle-treated, low and high dose budesonide-treated. First-order texture, geometrical features and features based on second-order statistics using run-length and grey-level co-occurrence matrices were calculated. Additionally, wavelet transforms were applied to compute first-order statistics on multiple scales. The texture analysis showed significant differences between the untreated and the budesonide-treated groups, which was in agreement with a biological marker.

1070. Influence of Drug Substances with Different Solubility on Hydration Processes of Prolonged Release Tablets

Anna Mlynarczyk¹, Marco Gruwel², Piotr Kulinowski¹, Krzysztof Jasinski¹, Przemyslaw Dorozynski³, Boguslaw Tomanek, ¹², Wladyslaw P. Weglarz<sup>1

1</sup>Department of Magnetic Resonance Imaging, Institute of Nuclear Physics Polish Academy of Sciences, Krakow, Poland; ²Institute for Biodiagnostics, National Research Council of Canada, Winnipeg, Manitoba, Canada; ³Department of Pharmaceutical Technology and Biopharmaceutics, Jagiellonian University, Krakow, Poland

MR microscopy was applied for study of water mobility and concentration in tablets, used for drug delivery. The tablets made of Hydroxypropylmethylcellulose-HPMC with and without addition of a drug substance of different solubility (L-dopa and ketoprofen) were tested. Two-dimensional T2 and proton density (PD) maps and their histograms were obtained. Spatial T2 and PD distributions along a tablet radius were also analyzed. Different mechanisms of tablets hydration were observed due to composition of the formulations, eg. for formulation with ketoprofen (low solubility of 0,24 mg/mL) the hydrogel formation was negligible.

1071. Hepato and Renal Protective Effect of Bacosides Against Aluminium Insult in Rats: A Proton Magnetic Resonance Study on Serum and Urine

Deepa Prajapati¹, Sundeep Tripathi², Sanjay Annarao¹, Abbas Ali Mahdi², Mahdi Hasan³, Farzana Mahdi⁴, Raja Roy¹, Chunni Lal Khetrapal<sup>1

1</sup>CBMR, Centre of Biomedical Magnetic Resonance, Lucknow, Uttar Pradesh, India; ²CSM Medical University; ³Anatomy, CSM Medical University; ⁴Era's Lucknow Medical College

Prolonged aluminium insults results in oxidative damage leading to hepato and nephrotoxicity in rats.1H NMR study of rat’s serum and urine have been utilized to evaluate the comparative protective effects of Bacosides and Donepezil drugs over continuous Aluminium- induced metabonomic changes. Bacosides was found to markedly attenuate the oxidative stress induced by Aluminium over long term (90 days) dose compared to Donepezil. This might be interpretated in terms of strong antioxidative property of Bacosides compared to Donepezil. The study suggests that 1H NMR metabolic profiling is an efficient method to explore the efficacy of potent molecules over metal toxicity.

1072. Effect of Varenicline on BOLD and Neural Networks in Awake Animals

Wei Chen¹, Joseph R. DiFranza², Jean King<sup>3

1</sup>Department of Psychiatry, University of Massachusetts Medical School, Worcester, MA, , United States; ²Department of Family Medicine and Community Health,, University of Massachusetts Medical School, Worcester; ³Department of Psychiatry, University of Massachusetts Medical School, Worcester, MA, United States

Tobacco dependence is the most preventable cause of death. Varenicline is a partial nicotinic receptor agonist/antagonist that is widely used as a smoking cessation aid. How varenicline helps smokers is unknown. We sought to compare the brain Blood oxygenation level dependent (BOLD) activation produced by a high dose varenicline to a lower dose varenicline in awake naïve animals, which hopes to assist in understanding the action of this partial nicotinic receptor agonist/antagonist in patients.