Webmaster's Note: As Gerard says below, his Fourth Report came in two parts
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- From Gerard
- Listing of Best Homolog
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Webmaster's Note: As Gerard says below, his Fourth Report came in two parts. I have incorporated the "Listing of Best Homolog" portion into this file, while the Excel Spreadsheet is accessible via a separate "click" on the main Genome page, following the “click” that got you to this document. From Gerard: "My fourth report is composed of 2 documents. Actually both were made by my colleague Michel Kress in Villejuif and myself. The first one "List of Physarum traces-hits" is a Microsoft Excel listing of 651 traces of the Physarum pilot assay, showing what we believe are 'significant homologies' to previously known proteins. This was done by comparing the 22,616 translated Physarum reads to the "nr" bank. The second document "listing of best homolog" shows for each of these reads an alignment corresponding to the best score. This second document is also helpful in obtaining the Ti number of each sequence. Example: You are interested by the PCNA homology of reads #33 of the Excel document. The read identity is POAA-aab24f11. Searching POAA-aab24f11 in the second document gives you access to the "ti" 818415229 code which you can use to get access to the sequence in the trace archive for a closer examination. We did not include the many reads showing homology to reverse transcriptase, integrase and the various polyproteins encoded by repeated retransposon-like sequences that are obviously abundant in the Physarum genome. The Excel document is presented sorted in alphabetical order of the organism with the closest homolog, to show that Dictyostelium is the closest relative. ******************************************************************************* Listing of Best Homolog gnl|ti|820216927 name:POAA-aab46c04.b1 mate_pair:818409684 (914 letters)
Sequences producing significant alignments: (bits) Value dbj|BAB33421.1| putative senescence-associated protein [Pis... 70 8e-011 ref|XP_676219.1| conserved hypothetical protein [Plasmodium... 46 0.002 gb|AAS66225.1| LRRG00134 [Rattus norvegicus] 37 1.3 gb|AAZ10060.1| hypothetical protein, conserved [Leishmania ... 36 2.1 ref|XP_414278.1| PREDICTED: similar to PHD finger protein 7... 36 2.1 ref|ZP_00345902.1| hypothetical protein Npun02000359 [Nosto... 34 8.1 >dbj|BAB33421.1| putative senescence-associated protein [Pisum sativum] Length = 282 Score = 70.5 bits (171), Expect = 8e-011 Identities = 43/77 (55%), Positives = 46/77 (59%) Frame = +2
SA T N FLSY +LL H GKTNLSHDGL PAHVP+ VNNPTL Sbjct: 34 SAMTNCANQRFLSYYVELLLRHCHQ---------WGKTNLSHDGLIPAHVPYWWVNNPTL 84
EFCF+ RADIEG K Sbjct: 85 GEFCFTMIGRADIEGSK 101 ----------------- gnl|ti|818409684 name:POAA-aab46c04.g1 mate_pair:820216927 (900 letters)
Score E
Sequences producing significant alignments: (bits) Value gb|AAK21974.1| rRNA promoter binding protein [Rattus norveg... 67 6e-010 ref|XP_578859.1| PREDICTED: similar to hypothetical protein... 67 8e-010 ref|XP_724980.1| hypothetical protein PY04651 [Plasmodium y... 60 8e-008 gb|EAA21327.1| putative senescence-associated protein [Plas... 52 4e-005 ref|XP_767406.1| hypothetical protein GLP_748_1200_211 [Gia... 50 8e-005 gb|AAS66225.1| LRRG00134 [Rattus norvegicus] 44 0.006 dbj|BAA10929.1| cytochrome P450 like_TBP [Nicotiana tabacum] 43 0.013 pir||A35664 PpoI endonuclease - slime mold (Physarum polyce... 40 0.14 gb|AAB65764.1| endonuclease [Physarum polycephalum] 40 0.14 pir||T02955 probable cytochrome P450 monooxygenase - maize ... 38 0.42 >gb|AAK21974.1| rRNA promoter binding protein [Rattus norvegicus] ref|NP_671477.1| rRNA promoter binding protein [Rattus norvegicus] Length = 295
Identities = 43/83 (51%), Positives = 48/83 (57%), Gaps = 1/83 (1%) Frame = -2
AS+ A PA L P+ TQP+EPILIPKLRIRLA+FPY PE HLGDLLRI Sbjct: 135 ASSSRGARPAPLRAPARPTQPLEPILIPKLRIRLADFPYLHCSNMPEAVHLGDLLRI--W 192
G +P F G R RT Sbjct: 193 VRPGARFTPSPPDFQGPARAHRT 215 ---------- gnl|ti|818409685 name:POAA-aab46c05.g1 mate_pair:820216928 (883 letters)
Score E
Sequences producing significant alignments: (bits) Value sp|P09353|SR3A_PHYPO Spherulin 3A (S3A) >gi|3114470|pdb|1AG... 58 4e-007 pdb|1HDF|B Chain B, Evolution Of The Eye Lens Beta-Gamma-Cr... 56 1e-006 gb|AAY42820.1| spherulin 3b [Physarum polycephalum] 54 9e-006 emb|CAG01157.1| unnamed protein product [Tetraodon nigrovir... 38 0.41 ref|NP_473177.2| hypothetical protein [Plasmodium falciparu... 34 7.7 ref|YP_003356.1| cytoplasmic membrane protein [Leptospira i... 34 7.7 ref|NP_714491.1| Peptidase family M23/M37 [Leptospira inter... 34 7.7 >sp|P09353|SR3A_PHYPO Spherulin 3A (S3A) pdb|1AG4| Nmr Structure Of Spherulin 3a (S3a) From Physarum Polycephalum, Minimized Average Structure gb|AAA29981.1| spherulin 2b precursor prf||1402254D spherulin 3a Length = 103
Identities = 30/30 (100%), Positives = 30/30 (100%) Frame = -2
LEESSQVTDLTTRNLNDAISSIIVATFESA Sbjct: 74 LEESSQVTDLTTRNLNDAISSIIVATFESA 103 ------------- gnl|ti|820216967 name:POAA-aab46g04.b1 mate_pair:818409721 (603 letters)
Score E
Sequences producing significant alignments: (bits) Value gb|AAO51154.1| similar to Oryza sativa (japonica cultivar-g... 68 2e-010 ref|XP_644233.1| hypothetical protein DDB0217574 [Dictyoste... 68 2e-010 ref|XP_478533.1| putative 1-acyl-sn-glycerol-3-phosphate ac... 65 2e-009 ref|NP_568925.1| acyltransferase [Arabidopsis thaliana] >gi... 62 1e-008 ref|XP_769292.1| hypothetical protein GLP_302_47616_48917 [... 41 0.023 gb|AAQ88467.1| EGAE2753 [Homo sapiens] >gi|16550554|dbj|BAB... 35 1.3 ref|XP_517181.1| PREDICTED: similar to hypothetical protein... 35 1.3 ref|XP_535637.2| PREDICTED: similar to CG3209-PB, isoform B... 35 1.3 dbj|BAD97263.1| hypothetical protein MGC11324 variant [Homo... 35 2.2 gb|AAH97362.1| Hypothetical protein LOC305166 [Rattus norve... 33 4.9 >gb|AAO51154.1| similar to Oryza sativa (japonica cultivar-group). P0681F05.34 protein [Dictyostelium discoideum] Length = 493
Identities = 27/38 (71%), Positives = 30/38 (78%) Frame = +2
L DEF+KCF S E AWNWNIYLYP WL G F+RYC+L Sbjct: 114 LDDEFSKCFNSPEIHAWNWNIYLYPGWLFGCFIRYCIL 151 Score = 51.2 bits (121), Expect = 2e-005 Identities = 22/37 (59%), Positives = 28/37 (75%) Frame = +3 Query: 189 VNASSWPIFTPTNSTEADEISKKIEEKLIKQLNEQTN 299 +N W + PTN++E DEISK IEEKL+KQLN+Q N Sbjct: 56 INQDPWSLLLPTNTSEIDEISKLIEEKLLKQLNKQVN 92 ----------- gnl|ti|818409828 name:POAA-aab47a12.b1 mate_pair:818410148 (869 letters)
Score E
Sequences producing significant alignments: (bits) Value ref|XP_643268.1| hypothetical protein DDB0169506 [Dictyoste... 66 2e-009 gb|AAU01379.1| MPXV-WRAIR169 [Monkeypox virus] 49 2e-004 gb|AAY97786.1| serine protease inhibitor-like SPI-1 protein... 49 2e-004 gb|AAY97588.1| serine protease inhibitor-like SPI-1 protein... 49 2e-004 gb|AAY97388.1| serine protease inhibitor-like SPI-1 protein... 49 2e-004 gb|AAY97186.1| serine protease inhibitor-like SPI-1 protein... 49 2e-004 gb|AAX09270.1| MPXV-COP-169 [Monkeypox virus] 49 2e-004 ref|XP_645916.1| hypothetical protein DDB0201701 [Dictyoste... 46 0.001 ref|XP_682005.1| hypothetical protein AN8736.2 [Aspergillus... 44 0.006 ref|NP_704458.1| hypothetical protein [Plasmodium falciparu... 42 0.021 >ref|XP_643268.1| hypothetical protein DDB0169506 [Dictyostelium discoideum] gb|AAS38755.1| similar to Dictyostelium discoideum (Slime mold). MkpA protein gb|EAL69352.1| hypothetical protein DDB0169506 [Dictyostelium discoideum] Length = 2473 Score = 65.9 bits (159), Expect = 2e-009 Identities = 56/221 (25%), Positives = 80/221 (36%), Gaps = 4/221 (1%) Frame = +1
VWVDLQDS+WD Y G ++G +I AL+ Y GITT Sbjct: 1316 VWVDLQDSIWDPYRGEMTKKGRIQNLIDALDHYHQEGITT-------------------- 1355
+Y+MG L+ + P CPTDR D+ + I +F Q++ K Sbjct: 1356 --------LYVMGLLEKLPSNQPLCPTDR---------DLANRLIGGDQMFSQLVNRAKQ 1398
I++ +L+ H +VCH Sbjct: 1399 L---------------GIKIVIDSTTRLSSKAAHRKYKGVVCH----------------- 1426
L N S S EF W +C +N RKL+ W++ V Sbjct: 1427 --TLDNNGQLSRLEGTDSSEFTWNDCVSMNMRKLSVWEMFV 1465 Score = 53.1 bits (126), Expect = 1e-005 Identities = 25/59 (42%), Positives = 36/59 (61%) Frame = +3 Query: 423 LGGEKDFLDLVAAAKARNIKLVVDSTSKLGARKAHRKYKALTCLSQDKKGYTIPAVGND 599 +GG++ F LV AK IK+V+DST++L ++ AHRKYK + C + D G G D Sbjct: 1383 IGGDQMFSQLVNRAKQLGIKIVIDSTTRLSSKAAHRKYKGVVCHTLDNNGQLSRLEGTD 1441 ------------- gnl|ti|818410148 name:POAA-aab47a12.g1 mate_pair:818409828 (830 letters)
Score E
Sequences producing significant alignments: (bits) Value ref|XP_643268.1| hypothetical protein DDB0169506 [Dictyoste... 70 1e-010 ref|XP_800514.1| PREDICTED: hypothetical protein XP_795421,... 60 7e-008 ref|XP_799835.1| PREDICTED: hypothetical protein XP_794742 ... 60 7e-008 dbj|BAC87222.1| unnamed protein product [Homo sapiens] 60 9e-008 gb|AAX27955.1| unknown [Schistosoma japonicum] 60 9e-008 ref|XP_800651.1| PREDICTED: hypothetical protein XP_795558 ... 60 9e-008 emb|CAE75230.1| Hypothetical protein CBG23181 [Caenorhabdit... 60 9e-008 ref|XP_331555.1| hypothetical protein [Neurospora crassa] >... 59 2e-007 ref|XP_646781.1| hypothetical protein DDB0191040 [Dictyoste... 59 2e-007 emb|CAE59812.1| Hypothetical protein CBG03276 [Caenorhabdit... 59 2e-007 >ref|XP_643268.1| hypothetical protein DDB0169506 [Dictyostelium discoideum] gb|AAS38755.1| similar to Dictyostelium discoideum (Slime mold). MkpA protein gb|EAL69352.1| hypothetical protein DDB0169506 [Dictyostelium discoideum] Length = 2473 Score = 69.7 bits (169), Expect = 1e-010 Identities = 30/50 (60%), Positives = 36/50 (72%) Frame = -3
+ WHD VFGFARF E I+AINF + D +FYVDCSPLAKI +DD + Sbjct: 1739 HATGWHDRVFGFARFSGNEVAIIAINFDNTDTEFYVDCSPLAKICSDDTI 1788 ----------- gnl|ti|818410149 name:POAA-aab47b01.g1 mate_pair:818409829 (870 letters)
Score E
Sequences producing significant alignments: (bits) Value emb|CAA10977.1| threonine deaminase [Arxula adeninivorans] ... 72 2e-011 emb|CAD60619.1| unnamed protein product [Podospora anserina] 67 8e-010 ref|ZP_00565150.1| Threonine dehydratase I [Methylobacillus... 66 1e-009 ref|XP_331226.1| hypothetical protein [Neurospora crassa] >... 66 1e-009 ref|NP_902763.1| threonine dehydratase [Chromobacterium vio... 65 2e-009 gb|EAA69706.1| hypothetical protein FG00296.1 [Gibberella z... 65 3e-009 emb|CAG99933.1| unnamed protein product [Kluyveromyces lact... 64 5e-009 ref|XP_502328.1| hypothetical protein [Yarrowia lipolytica]... 64 5e-009 ref|ZP_00775746.1| Threonine dehydratase I [Pseudoalteromon... 64 5e-009 ref|XP_367299.1| hypothetical protein MG07224.4 [Magnaporth... 64 7e-009 >emb|CAA10977.1| threonine deaminase [Arxula adeninivorans] sp|O42615|THDH_ARXAD Threonine dehydratase, mitochondrial precursor (Threonine deaminase) Length = 550 Score = 72.0 bits (175), Expect = 2e-011 Identities = 31/45 (68%), Positives = 41/45 (91%) Frame = -3
+G+FADGAAVK++GE TF++C+QVVDE+VLV+ DE+CAAIK FT Sbjct: 261 VGLFADGAAVKILGEETFRLCQQVVDEIVLVSTDEICAAIKDVFT 305 --------------------- gnl|ti|818410159 name:POAA-aab47b11.g1 mate_pair:818409839 (864 letters)
Score E
Sequences producing significant alignments: (bits) Value ref|XP_781611.1| PREDICTED: similar to RAB1, member RAS onc... 59 3e-007 emb|CAG13909.1| unnamed protein product [Tetraodon nigrovir... 55 3e-006 dbj|BAD87657.1| Ras-related protein RIC1 [Oryza sativa (jap... 53 1e-005 gb|AAW46571.1| ras-related protein ypt1, putative [Cryptoco... 53 1e-005 ref|NP_957436.1| hypothetical protein LOC394117 [Danio reri... 53 1e-005 ref|NP_001028800.1| GTP binding protein Rab1a [Bos taurus] ... 53 1e-005 ref|NP_918377.1| putative RIC1_ORYSA RAS-RELATED PROTEIN RI... 53 1e-005 ref|XP_515516.1| PREDICTED: hypothetical protein XP_515516 ... 53 1e-005 ref|NP_914429.1| putative GTP-binding protein [Oryza sativa... 53 1e-005 gb|AAV38334.1| RAB1A, member RAS oncogene family [synthetic... 53 1e-005 >ref|XP_781611.1| PREDICTED: similar to RAB1, member RAS oncogene family [Strongylocentrotus purpuratus] Length = 218
Identities = 29/34 (85%), Positives = 30/34 (88%) Frame = -2
P+SDYLFKLLLIGDSGVGKSCLLLRFAV L S Sbjct: 3 PNSDYLFKLLLIGDSGVGKSCLLLRFAVISSLFS 36 Score = 38.9 bits (89), Expect = 0.23 Identities = 22/36 (61%), Positives = 25/36 (69%), Gaps = 1/36 (2%) Frame = -3 Query: 337 LVRFI*CLMIFSCSLILF*-DDTYTESYISTIGVDF 233 L+RF +FS L + DDTYTESYISTIGVDF Sbjct: 25 LLRFAVISSLFSLFLSFYLQDDTYTESYISTIGVDF 60 >emb|CAG13909.1| unnamed protein product [Tetraodon nigroviridis] Length = 59
Identities = 26/27 (96%), Positives = 26/27 (96%) Frame = -2
P SDYLFKLLLIGDSGVGKSCLLLRFA Sbjct: 1 PHSDYLFKLLLIGDSGVGKSCLLLRFA 27 Score = 43.5 bits (101), Expect = 0.009 Identities = 20/24 (83%), Positives = 21/24 (87%) Frame = -3 Query: 304 SCSLILF*DDTYTESYISTIGVDF 233 SC L+ F DDTYTESYISTIGVDF Sbjct: 20 SCLLLRFADDTYTESYISTIGVDF 43 ------------- gnl|ti|818409842 name:POAA-aab47c03.b1 mate_pair:818410161 (915 letters)
Score E
Sequences producing significant alignments: (bits) Value gb|AAX30301.1| unknown [Schistosoma japonicum] 87 8e-016 emb|CAH00945.1| unnamed protein product [Kluyveromyces lact... 86 2e-015 dbj|BAD26579.1| cytochrome P450 like_TBP [Citrullus lanatus] 74 5e-012 gb|AAX27748.1| unknown [Schistosoma japonicum] 69 3e-010 pir||T02955 probable cytochrome P450 monooxygenase - maize ... 66 2e-009 emb|CAB51041.1| putative transcription factor [Periplaneta ... 65 3e-009 ref|XP_578859.1| PREDICTED: similar to hypothetical protein... 65 3e-009 dbj|BAA10929.1| cytochrome P450 like_TBP [Nicotiana tabacum] 63 1e-008 gb|EAA16545.1| hypothetical protein [Plasmodium yoelii yoelii] 54 6e-006 gb|EAA21327.1| putative senescence-associated protein [Plas... 47 7e-004 >gb|AAX30301.1| unknown [Schistosoma japonicum] Length = 55 Score = 87.0 bits (214), Expect = 8e-016 Identities = 40/44 (90%), Positives = 41/44 (93%) Frame = +2
LI DAHEW+NEIPTVPTYYLAKPQPRERAW N RGKKTLLSLTL Sbjct: 5 LISDAHEWMNEIPTVPTYYLAKPQPRERAWRNQRGKKTLLSLTL 48 ---------- gnl|ti|818410192 name:POAA-aab47f04.g1 (902 letters) Score E
Sequences producing significant alignments: (bits) Value gb|AAF56326.1| CG13628-PA [Drosophila melanogaster] >gi|546... 60 1e-007 gb|ABA91866.1| dna-directed rna polymerase ii 8.2 kda polyp... 59 2e-007 gb|EAA00426.2| ENSANGP00000020255 [Anopheles gambiae str. P... 59 2e-007 ref|NP_176363.1| DNA-directed RNA polymerase [Arabidopsis t... 59 2e-007 ref|XP_577644.1| PREDICTED: similar to Ab1-108 [Rattus norv... 59 2e-007 gb|AAP92539.1| Ab1-108 [Rattus norvegicus] 59 3e-007 ref|NP_001016343.1| hypothetical protein LOC549097 [Xenopus... 59 3e-007 ref|XP_874715.1| PREDICTED: similar to DNA-directed RNA pol... 59 3e-007 ref|XP_521707.1| PREDICTED: similar to Ab1-108 [Pan troglod... 59 3e-007 ref|XP_697922.1| PREDICTED: similar to DNA-directed RNA pol... 59 3e-007 >gb|AAF56326.1| CG13628-PA [Drosophila melanogaster] gb|EAL26851.1| GA12420-PA [Drosophila pseudoobscura] gb|AAM51044.1| SD08670p [Drosophila melanogaster] sp|Q9VC49|RPB10_DROME DNA-directed RNA polymerase II 7.6 kDa polypeptide (RPB10) (RPB7.6) ref|NP_651280.1| CG13628-PA [Drosophila melanogaster] Length = 67 Score = 59.7 bits (143), Expect = 1e-007 Identities = 24/27 (88%), Positives = 27/27 (100%) Frame = +1
RCFTCGKVIGNKW++YLGLLQA+YTEG Sbjct: 6 RCFTCGKVIGNKWESYLGLLQAEYTEG 32 --------- gnl|ti|818409881 name:POAA-aab47g01.b1 mate_pair:818410200 (900 letters)
Score E
Sequences producing significant alignments: (bits) Value gb|AAG41774.1| Cat [Promoter probe vector pPR9TT] 466 e-130 gb|ABB17069.1| chloramphenicol acetyl transferase [Moss gen... 465 e-129 ------------- gnl|ti|818410200 name:POAA-aab47g01.g1 mate_pair:818409881 (884 letters) Score E
Sequences producing significant alignments: (bits) Value sp|P03851|YPB3_ECOLI Hypothetical 9.4 kDa protein 179 1e-043 gb|AAB59124.1| 10.2 kd ORF [Plasmid ColE1] >gi|9507257|ref|... 122 1e-026 pir||JQ1541 hypothetical 16.9K protein - Salmonella typhimu... 118 3e-025 gb|AAR02632.1| conserved hypothetical protein [Salmonella t... 93 1e-017 ref|YP_355421.1| hypothetical protein pCollet_06 [Escherich... 90 9e-017 ref|NP_758764.1| hypothetical protein pEA28_01 [Erwinia amy... 90 1e-016 gb|AAB59125.1| 13.8 kd ORF [Plasmid ColE1] >gi|9507258|ref|... 89 2e-016 ref|ZP_00374729.1| conserved hypothetical protein [Wolbachi... 81 5e-014 ref|NP_995570.1| hypothetical protein pPCP04 [Yersinia pest... 74 7e-012 sp|P03845|YPA1_ECOLI Hypothetical protein 1 72 3e-011 >sp|P03851|YPB3_ECOLI Hypothetical 9.4 kDa protein Length = 84 Score = 179 bits (453), Expect = 1e-043 Identities = 83/84 (98%), Positives = 84/84 (100%) Frame = -3
+HEPPVQPDRCALSGNYRLESNPVRHDLSPLAAATGNRISRARYVGGATEFLKWWPNYGY Sbjct: 1 MHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAATGNRISRARYVGGATEFLKWWPNYGY 60
TRRTVFGICALLKPVTFGKRVGSS Sbjct: 61 TRRTVFGICALLKPVTFGKRVGSS 84 -----
gnl|ti|818410208 name:POAA-aab47g10.g1 mate_pair:818409889 (903 letters)
Score E
Sequences producing significant alignments: (bits) Value gb|AAG22284.1| CG12352-PA [Drosophila melanogaster] >gi|179... 65 4e-009 dbj|BAD52531.1| Mak3 protein-like protein [Oryza sativa (ja... 64 6e-009 gb|EAL26071.1| GA11576-PA [Drosophila pseudoobscura] 64 7e-009 ref|NP_196695.1| N-acetyltransferase [Arabidopsis thaliana]... 64 9e-009 gb|AAH78316.1| Mak3 homolog [Danio rerio] >gi|57525643|ref|... 64 9e-009 emb|CAG04228.1| unnamed protein product [Tetraodon nigrovir... 63 2e-008 ref|XP_595378.1| PREDICTED: similar to Mak3 homolog, partia... 62 3e-008 ref|XP_526267.1| PREDICTED: similar to Mak3 homolog [Pan tr... 62 3e-008 dbj|BAE40319.1| unnamed protein product [Mus musculus] 62 3e-008 dbj|BAE39286.1| unnamed protein product [Mus musculus] 62 3e-008 >gb|AAG22284.1| CG12352-PA [Drosophila melanogaster] gb|AAL48448.1| AT27602p [Drosophila melanogaster] gb|AAF34715.1| separation anxiety protein [Drosophila melanogaster] sp|Q9NHD5|SAN_DROME Probable acetyltransferase san (Separation anxiety protein) ref|NP_524779.1| CG12352-PA [Drosophila melanogaster] Length = 184 Score = 64.7 bits (156), Expect = 4e-009 Identities = 44/115 (38%), Positives = 55/115 (47%), Gaps = 3/115 (2%) Frame = -2
YNDKFY V + G KL Y YNDI VGAVCCR+ E Sbjct: 31 YNDKFY---VDVLEAGELAKLAY-------------------YNDIVVGAVCCRIDNTEN 68
+ ++YIMTLG L+PYR+L IG +F + D G S+F HV I+ Sbjct: 69 QRRLYIMTLGCLSPYRRLGIGTVMFEHIMNFAEKD-------GNFDSIFLHVQIN 116 Score = 60.5 bits (145), Expect = 8e-008 Identities = 40/115 (34%), Positives = 53/115 (46%) Frame = -3 Query: 469 GTELLEYVINLCKKHPQVQQIYPAMVFFLDFKTY*LTVSTYLHVQVNNDAAIEFYKRFGF 290 GT + E+++N +K I+ LHVQ+NN+ AIEFYK+FGF Sbjct: 89 GTVMFEHIMNFAEKDGNFDSIF-------------------LHVQINNNGAIEFYKKFGF 129
EI +T + Y KRIEP D +VL KTL + TAPN Sbjct: 130 EIVDTKEQYY----------------------KRIEPADAHVLQKTLRR--TAPN 160 ------------------ gnl|ti|818410210 name:POAA-aab47g12.g1 mate_pair:818409891 (756 letters)
Score E
Sequences producing significant alignments: (bits) Value gb|AAL31950.1| CDH1-D [Gallus gallus] 85 2e-015 ref|XP_428325.1| PREDICTED: similar to Ac1147, partial [Gal... 62 2e-008 gb|AAP92584.1| Ab2-057 [Rattus norvegicus] >gi|32492570|gb|... 57 5e-007 ref|XP_520857.1| PREDICTED: hypothetical protein XP_520857 ... 57 5e-007 ref|XP_358030.2| PREDICTED: similar to Ac1147 [Mus musculus] 57 8e-007 ref|XP_578848.1| PREDICTED: similar to Ac1147 [Rattus norve... 43 0.010 ref|XP_578845.1| PREDICTED: similar to Ac1147 [Rattus norve... 43 0.010 ref|XP_578905.1| PREDICTED: similar to Ac1147 [Rattus norve... 41 0.048 ref|XP_578906.1| PREDICTED: similar to Ac1147 [Rattus norve... 39 0.14 ref|XP_578847.1| PREDICTED: similar to Ac1147 [Rattus norve... 39 0.14 >gb|AAL31950.1| CDH1-D [Gallus gallus] Length = 451 Score = 85.1 bits (209), Expect = 2e-015 Identities = 61/132 (46%), Positives = 71/132 (53%), Gaps = 1/132 (0%) Frame = +1
NDP+AGSPT TLLRLLLPL V + + V P L +PIGS+DGRCVQR Sbjct: 250 NDPSAGSPTETLLRLLLPLDSQVRPSSRRSGRAVADPAGADPRTSLNHPIGSSDGRCVQR 309
AGT+ST+ D LL IPR IIA P G PR +GT S C+ R Sbjct: 310 AGTYSTRAYDPHLLGIPRSWGIIAIPDPHHEWGSTGYPRLPAKGTHKLSQSVQRACSPRT 369
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