Wilhelm bernhard workshop on the cell nucleus

Marco Biggiogera

International Committee

F. Amalric, France

M. Biggiogera, Italy

C. de la Torre, Spain

E. Egyhazi, Sweden

S. Fakan, Switzerland

R. Hancock, Canada

D. Hernandez-Verdun, France

E.J. Hidvegi, Hungary

P. Hozak, Czech Republic

E.G. Jordan, UK

S. Moreno Diaz de la Espina, Spain

M. Muramatsu, Japan

A. Pombo, Portugal

L. Rothblum, USA

U. Scheer, Germany

M. Schmidt-Zachmann, Germany

C. Sekeris, Greece

K. Smetana, Czech Republic

J. Sommerville, UK

R.M. Tanguay, Canada

M. Thiry, Belgium

F. Wachtler, Austria

P. Widlak, Poland

O. Zatsepina, Russia


PREVIOUS WORKSHOPS

1969 Liblice, Czechoslovakia 1987 Stevensbeek, The Netherlands

1971 Lake Balaton, Hungary 1989 Suzdal, USSR

1973 Abisko, Sweden 1991 Les Diablerets, Switzerland

1975 Varna, Bulgary 1993 Balatonaliga, Hungary

1977 Salamanca, Spain 1995 Spa, Belgium

1979 Weimar, Germany 1998 Lac-Delage, Canada

1981 Safed, Israel 1999 Prague, Czech Republic

1983 Banyuls-sur-Mer, France 2001 Arcachon, France

1985 Krakow, Poland

Local Organizing Committee

Marco Biggiogera - President

Vittorio Bertone

Teresa Bosini

Maria Grazia Bottone

Francine Flach

Daniela Necchi

Carlo Pellicciari

Francesca Ronchetti

Elda Scherini

Cristiana Soldani

Lorella Vecchio

Paola Veneroni

CONTENTS

• 
  Program p. 4
  
• 
  Scientific sessions p. 5
  
• 
  Wilhelm Bernhard Lecture p. 9
  
• 
  Oral Presentations
  

• 
  Transcription and splicing p. 11
  
• 
  Gene expression and nucleus-to-cytoplasm transport p. 15
  
• 
  Nucleolus p. 18
  
• 
  Cell death and nuclear markers of disease p. 23
  
• 
  Wilhelm Bernhard Junior Lecture p. 26
  
• 
  Cell cycle p. 27
  
• 
  Chromatin p. 29
  
• 
  Nuclear proteins, matrix and bodies p. 31
  

• Posters p. 34

• Author index & Addresses p. 45

18^th Wilhelm Bernhard Workshop on the cell nucleus – Program

Day	Morning	Afternoon	Evening
Sept. 4	Registration	Opening ceremony Wilhelm Bernhard Lecture Stan Fakan Visit to the Museum for the History of the University of Pavia	Cocktail
Sept. 5	Transcription and splicing 1 (Chairperson: G. Vazquez-Nin – A. Kumar) Coffee break Transcription and splicing 2 (Chairperson: G. Vazquez-Nin – A. Kumar)	Gene expression and nucleus to cytoplasm transport (Chairperson: L. Rothblum – R. Hancock ) Coffee break Poster session
Sept. 6	Nucleolus 1 (Chairperson: T. Pederson – J. Sommerville) Coffee break Nucleolus 2 (Chairperson: T. Pederson – J. Sommerville)	Excursion	Social dinner
Sept. 7	Cell death and nuclear markers of disease (Chairperson: M.C. Dabauvalle – E. Falcieri) Coffee break Wilhelm Bernhard Junior Lecture	Cell cycle (Chairperson:E. Prosperi – I. Raska) Coffee break Round table Committee meeting	Concert & Farewell party
Sept. 8	Nuclear proteins, matrix and bodies (Chairperson: O. Zatsepina – R. Benavente) Coffee break Chromatin (Chairperson: W. Hennig – P. Debey) Closing ceremony

18^th Wilhelm Bernhard Workshop on the cell nucleus – Scientific sessions

September 4^th 5 pm Aula Scarpa, University	WILHELM BERHNARD LECTURE page 9 Ultrastructural analysis is a major tool in investigating the nuclear functional architecture Stan Fakan
September 5 8.30- 13 am Collegio Nuovo	TRANSCRIPTION AND SPLICING page 11 NUCLEAR TRANSLATION. AN OLD STORY Iborra F.J. and Cook P.R. RECOGNITION OF OPEN READING FRAMES IN pre-mRNAs WITHIN THE CELL NUCLEUS Sperling R. and Sperling J. EVIDENCES SUGGESTING THAT TRANS-CRIPTION IS INVOLVED IN HOMOLOGUE CHROMOSOME RECOGNITION AND ALIGNMENT PREVIOUS TO PAIRING IN EARLY MEIOTIC PROPHASE Vázquez-Nin G, Echeverría O., Ortiz R., Scassellati C., Ubaldo E., and Fakan S. ß-Actin, TAta binding protein (TBP) and transcription by RNA polymerase II (RNAPII) de Lanerolle P., Mavrommatis E., Johnson, T., Hoffman W., Stojiljkovic L., Fuchsova B., Philimonenko V. and Hozak P. REGULATION OF PROCESSIVE TRANSCRIPTION Zhou M., Deng L.^, Kashanchi F., Shatkin A. and Kumar A. THE MULTIFUNCTIONAL TUMOUR SUPPRESSOR ZINC FINGER PROTEIN WT1 BINDS TO TRANSCRIPTS IN VIVO Ladomery M., Hastie N., Woolner S., Slight J. and Sommerville J. THE DEAD BOX RNA HELICASE P68: A TRANSCRIPTIONAL CO-ACTIVATOR AND REPRESSOR Bates G., Wilson, B., Nicol S. and Fuller-Pace F. THE SUPRASPLICEOSOME: A MACRO-MOLECULAR MACHINE FOR MAKING FUNCTIONAL mRNA Sperling J. and Sperling R. CDC2L5, A NUCLEAR CDC2-RELATED KINASE CONTAINING AN RS DOMAIN IS INVOLVED IN PRE-MRNA SPLICING Durieux S.#, Even Y., Géraud M.L., Weil D.*, Genevière A.M. MODULATION OF TRANSCRIPTION IN WCH-17 CELLS AFTER HYPOTERMIC STIMULUS Vecchio L.^1, Baldelli B.^2, Malatesta M.^2and Biggiogera M.^1
September 5 2.30 – 4.30 pm Collegio Nuovo September 5 2.30 – 4.30 pm Collegio Nuovo 5-7 pm Collegio Volta	GENE EXPRESSION & NUCLEUS-TO-CYTOPLASM TRANSPORT page 15 TOPOLOGICAL ORGANIZATION OF GENE EXPRESSION IN DINOFLAGELLATES Alverca E, Franca S and Moreno Díaz de la Espina, S. HSP70 AND HSP90 ARE DIFFERENTIALLY EXPRESSED AND RELOCATED IN THE NUCLEUS AFTER INDUCTION OF MICRO-SPORE EMBRYOGENESIS BY STRESS Seguí J.M., Testillano P.S., and Risueño M.C. Lack of epigenetic reprogramming in early sheep embryos produced by somatic cell nuclear transfer Beaujean N., Taylor J., Gardner J., Wilmut I., Meehan R. and Young L. MAMMALIAN RRN3 BECOMES INACTIVATED IN THE PROCESS OF TRANSCRIPTION Hirschler-Laszkiewicz, I., Cavanaugh, A., and Rothblum, L. ACTIVE MAPKs TRANSLOCATE TO THE NUCLEUS AND ARE TARGETED TO DEFINED DOMAINS DURING PLANT DIFFERENTIA-TION AND PROLIFERATION Coronado M.J., Seguí J.M., González-Melendi P., Ramírez C., Barany, I., Testillano P.S., and Risueño M.C. Enhancement of nuclear uptake of therapeutic genes and macro-molecules by using NLS and PTD containing viral peptides Sanders N, Vandenbroucke R, De Smedt S, Vanderleyden E, Lucas B, Remaut K Demeester J evidence that The nuclear lamina is an elastic structure which exerts pressure on intranuclear macro-molecules Hancock R. POSTER SESSION page 34
September 6^th 8.30- 13 am Collegio Nuovo September 6^th 8.30- 13 am Collegio Nuovo	NUCLEOLUS page 18 UBF INDUCES CHROMATIN REMODELING Chen D., Belmont A. S. and Huang S. ARTIFICIALLY UNRAVELED NUCLEOLI AS A MODEL TO STUDY THE NUCLEOLUS FUNCTIONAL ORGANIZATION Dudnik O.A. and Smirnova O.Iu. EFFECT OF ACTINOMYCIN-D UPON UP-STREAM BINDING FACTOR LOCALIZATION: A (3D + TIME) STUDY Elias E., Tchélidzé P., Benassarou A., Sauvage C., Bobichon H., Ploton D. and O’Donohue M.F. CONNECTION OF NUCLEOLAR TRANS-CRIPTION AND PROCESSING MACHINERY IS ENERGY-DEPENDENT Louvet E., Junéra H.R., Le Panse S. and Hernandez-Verdun D. TOPOGRAPHY OF RIBOSOMAL DNA (rDNA) DETECTABLE BY FISH IN EARLY MOUSE EMBRYOS Korobova F.V., Noniashvili E.M., Schoefer C., Romanova L.G., Wachtler F., Dyban A.P., and Zatsepina O.V.