Wilhelm bernhard workshop on the cell nucleus



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Marco Biggiogera




International Committee

F. Amalric, France

M. Biggiogera, Italy

C. de la Torre, Spain

E. Egyhazi, Sweden

S. Fakan, Switzerland

R. Hancock, Canada

D. Hernandez-Verdun, France

E.J. Hidvegi, Hungary

P. Hozak, Czech Republic

E.G. Jordan, UK

S. Moreno Diaz de la Espina, Spain

M. Muramatsu, Japan

A. Pombo, Portugal

L. Rothblum, USA

U. Scheer, Germany

M. Schmidt-Zachmann, Germany

C. Sekeris, Greece

K. Smetana, Czech Republic

J. Sommerville, UK

R.M. Tanguay, Canada

M. Thiry, Belgium

F. Wachtler, Austria

P. Widlak, Poland



O. Zatsepina, Russia


PREVIOUS WORKSHOPS

1969 Liblice, Czechoslovakia 1987 Stevensbeek, The Netherlands


1971 Lake Balaton, Hungary 1989 Suzdal, USSR

1973 Abisko, Sweden 1991 Les Diablerets, Switzerland

1975 Varna, Bulgary 1993 Balatonaliga, Hungary

1977 Salamanca, Spain 1995 Spa, Belgium

1979 Weimar, Germany 1998 Lac-Delage, Canada

1981 Safed, Israel 1999 Prague, Czech Republic

1983 Banyuls-sur-Mer, France 2001 Arcachon, France

1985 Krakow, Poland




Local Organizing Committee



Marco Biggiogera - President

Vittorio Bertone

Teresa Bosini

Maria Grazia Bottone

Francine Flach

Daniela Necchi

Carlo Pellicciari

Francesca Ronchetti

Elda Scherini

Cristiana Soldani

Lorella Vecchio

Paola Veneroni





CONTENTS




  • Program p. 4

  • Scientific sessions p. 5

  • Wilhelm Bernhard Lecture p. 9

  • Oral Presentations

    • Transcription and splicing p. 11

    • Gene expression and nucleus-to-cytoplasm transport p. 15

    • Nucleolus p. 18

    • Cell death and nuclear markers of disease p. 23

    • Wilhelm Bernhard Junior Lecture p. 26

    • Cell cycle p. 27

    • Chromatin p. 29

    • Nuclear proteins, matrix and bodies p. 31
  • Posters p. 34

  • Author index & Addresses p. 45


18th Wilhelm Bernhard Workshop on the cell nucleus – Program



Day

Morning



Afternoon


Evening


Sept. 4

Registration

Opening ceremony
Wilhelm Bernhard Lecture

Stan Fakan
Visit to the Museum for the History of the University of Pavia

Cocktail

Sept. 5

Transcription and splicing 1

(Chairperson: G. Vazquez-Nin – A. Kumar)


Coffee break
Transcription and splicing 2

(Chairperson: G. Vazquez-Nin – A. Kumar)

Gene expression and nucleus to cytoplasm transport

(Chairperson: L. Rothblum – R. Hancock )
Coffee break
Poster session




Sept. 6

Nucleolus 1

(Chairperson: T. Pederson – J. Sommerville)
Coffee break
Nucleolus 2

(Chairperson: T. Pederson – J. Sommerville)

Excursion


Social dinner

Sept. 7

Cell death and nuclear markers of disease

(Chairperson: M.C. Dabauvalle – E. Falcieri)
Coffee break
Wilhelm Bernhard Junior Lecture

Cell cycle

(Chairperson:E. Prosperi – I. Raska)
Coffee break
Round table

Committee meeting

Concert

&

Farewell party

Sept. 8

Nuclear proteins, matrix and bodies

(Chairperson: O. Zatsepina – R. Benavente)

Coffee break



Chromatin

(Chairperson: W. Hennig – P. Debey)
Closing ceremony









18th Wilhelm Bernhard Workshop on the cell nucleus – Scientific sessions


September 4th

5 pm
Aula Scarpa, University





WILHELM BERHNARD LECTURE page 9
Ultrastructural analysis is a major tool in investigating the nuclear functional architecture

Stan Fakan




September 5

8.30- 13 am

Collegio Nuovo


TRANSCRIPTION AND SPLICING page 11

NUCLEAR TRANSLATION. AN OLD STORY


Iborra F.J. and Cook P.R.
RECOGNITION OF OPEN READING FRAMES IN pre-mRNAs WITHIN THE CELL NUCLEUS

Sperling R. and Sperling J.


EVIDENCES SUGGESTING THAT TRANS-CRIPTION IS INVOLVED IN HOMOLOGUE CHROMOSOME RECOGNITION AND ALIGNMENT PREVIOUS TO PAIRING IN EARLY MEIOTIC PROPHASE

Vázquez-Nin G, Echeverría O., Ortiz R., Scassellati C., Ubaldo E., and Fakan S.
ß-Actin, TAta binding protein (TBP) and transcription by RNA polymerase II (RNAPII)

de Lanerolle P., Mavrommatis E., Johnson, T., Hoffman W., Stojiljkovic L., Fuchsova B., Philimonenko V. and Hozak P.

REGULATION OF PROCESSIVE TRANSCRIPTION


Zhou M., Deng L., Kashanchi F., Shatkin A. and Kumar A.
THE MULTIFUNCTIONAL TUMOUR SUPPRESSOR ZINC FINGER PROTEIN WT1 BINDS TO TRANSCRIPTS IN VIVO

Ladomery M., Hastie N., Woolner S., Slight J. and Sommerville J.
THE DEAD BOX RNA HELICASE P68: A TRANSCRIPTIONAL CO-ACTIVATOR AND REPRESSOR

Bates G., Wilson, B., Nicol S. and Fuller-Pace F.


THE SUPRASPLICEOSOME: A MACRO-MOLECULAR MACHINE FOR MAKING FUNCTIONAL mRNA

Sperling J. and Sperling R.


CDC2L5, A NUCLEAR CDC2-RELATED KINASE CONTAINING AN RS DOMAIN IS INVOLVED IN PRE-MRNA SPLICING

Durieux S.#, Even Y., Géraud M.L., Weil D.*, Genevière A.M.


MODULATION OF TRANSCRIPTION IN WCH-17 CELLS AFTER HYPOTERMIC STIMULUS

Vecchio L.1, Baldelli B.2, Malatesta M.2 and Biggiogera M.1



September 5

2.30 – 4.30 pm

Collegio Nuovo

September 5

2.30 – 4.30 pm

Collegio Nuovo

5-7 pm

Collegio Volta



GENE EXPRESSION &

NUCLEUS-TO-CYTOPLASM TRANSPORT page 15
TOPOLOGICAL ORGANIZATION OF GENE EXPRESSION IN DINOFLAGELLATES

Alverca E, Franca S and Moreno Díaz de la Espina, S.
HSP70 AND HSP90 ARE DIFFERENTIALLY EXPRESSED AND RELOCATED IN THE NUCLEUS AFTER INDUCTION OF MICRO-SPORE EMBRYOGENESIS BY STRESS

Seguí J.M., Testillano P.S., and Risueño M.C.


Lack of epigenetic reprogramming in early sheep embryos produced by somatic cell nuclear transfer

Beaujean N., Taylor J., Gardner J., Wilmut I., Meehan R. and Young L.
MAMMALIAN RRN3 BECOMES INACTIVATED IN THE PROCESS OF TRANSCRIPTION

Hirschler-Laszkiewicz, I., Cavanaugh, A., and Rothblum, L.


ACTIVE MAPKs TRANSLOCATE TO THE NUCLEUS AND ARE TARGETED TO DEFINED DOMAINS DURING PLANT DIFFERENTIA-TION AND PROLIFERATION

Coronado M.J., Seguí J.M., González-Melendi P., Ramírez C., Barany, I., Testillano P.S., and Risueño M.C.


Enhancement of nuclear uptake of therapeutic genes and macro-molecules by using NLS and PTD containing viral peptides

Sanders N, Vandenbroucke R, De Smedt S, Vanderleyden E, Lucas B, Remaut K Demeester J
evidence that The nuclear lamina is an elastic structure which exerts pressure on intranuclear macro-molecules

Hancock R.

POSTER SESSION page 34

September 6th

8.30- 13 am

Collegio Nuovo

September 6th

8.30- 13 am

Collegio Nuovo



NUCLEOLUS page 18
UBF INDUCES CHROMATIN REMODELING

Chen D., Belmont A. S. and Huang S.


ARTIFICIALLY UNRAVELED NUCLEOLI AS A MODEL TO STUDY THE NUCLEOLUS FUNCTIONAL ORGANIZATION

Dudnik O.A. and Smirnova O.Iu.


EFFECT OF ACTINOMYCIN-D UPON UP-STREAM BINDING FACTOR LOCALIZATION: A (3D + TIME) STUDY

Elias E., Tchélidzé P., Benassarou A., Sauvage C., Bobichon H., Ploton D. and O’Donohue M.F.


CONNECTION OF NUCLEOLAR TRANS-CRIPTION AND PROCESSING MACHINERY IS ENERGY-DEPENDENT

Louvet E., Junéra H.R., Le Panse S. and Hernandez-Verdun D.
TOPOGRAPHY OF RIBOSOMAL DNA (rDNA) DETECTABLE BY FISH IN EARLY MOUSE EMBRYOS

Korobova F.V., Noniashvili E.M., Schoefer C., Romanova L.G., Wachtler F., Dyban A.P., and Zatsepina O.V.


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