Wilhelm bernhard workshop on the cell nucleus

18^th Wilhelm Bernhard Workshop on the cell nucleus – Scientific sessions

September 4^th 5 pm Aula Scarpa, University	WILHELM BERHNARD LECTURE page 9 Ultrastructural analysis is a major tool in investigating the nuclear functional architecture Stan Fakan
September 5 8.30- 13 am Collegio Nuovo	TRANSCRIPTION AND SPLICING page 11 NUCLEAR TRANSLATION. AN OLD STORY Iborra F.J. and Cook P.R. RECOGNITION OF OPEN READING FRAMES IN pre-mRNAs WITHIN THE CELL NUCLEUS Sperling R. and Sperling J. EVIDENCES SUGGESTING THAT TRANS-CRIPTION IS INVOLVED IN HOMOLOGUE CHROMOSOME RECOGNITION AND ALIGNMENT PREVIOUS TO PAIRING IN EARLY MEIOTIC PROPHASE Vázquez-Nin G, Echeverría O., Ortiz R., Scassellati C., Ubaldo E., and Fakan S. ß-Actin, TAta binding protein (TBP) and transcription by RNA polymerase II (RNAPII) de Lanerolle P., Mavrommatis E., Johnson, T., Hoffman W., Stojiljkovic L., Fuchsova B., Philimonenko V. and Hozak P. REGULATION OF PROCESSIVE TRANSCRIPTION Zhou M., Deng L.^, Kashanchi F., Shatkin A. and Kumar A. THE MULTIFUNCTIONAL TUMOUR SUPPRESSOR ZINC FINGER PROTEIN WT1 BINDS TO TRANSCRIPTS IN VIVO Ladomery M., Hastie N., Woolner S., Slight J. and Sommerville J. THE DEAD BOX RNA HELICASE P68: A TRANSCRIPTIONAL CO-ACTIVATOR AND REPRESSOR Bates G., Wilson, B., Nicol S. and Fuller-Pace F. THE SUPRASPLICEOSOME: A MACRO-MOLECULAR MACHINE FOR MAKING FUNCTIONAL mRNA Sperling J. and Sperling R. CDC2L5, A NUCLEAR CDC2-RELATED KINASE CONTAINING AN RS DOMAIN IS INVOLVED IN PRE-MRNA SPLICING Durieux S.#, Even Y., Géraud M.L., Weil D.*, Genevière A.M. MODULATION OF TRANSCRIPTION IN WCH-17 CELLS AFTER HYPOTERMIC STIMULUS Vecchio L.^1, Baldelli B.^2, Malatesta M.^2and Biggiogera M.^1
September 5 2.30 – 4.30 pm Collegio Nuovo September 5 2.30 – 4.30 pm Collegio Nuovo 5-7 pm Collegio Volta	GENE EXPRESSION & NUCLEUS-TO-CYTOPLASM TRANSPORT page 15 TOPOLOGICAL ORGANIZATION OF GENE EXPRESSION IN DINOFLAGELLATES Alverca E, Franca S and Moreno Díaz de la Espina, S. HSP70 AND HSP90 ARE DIFFERENTIALLY EXPRESSED AND RELOCATED IN THE NUCLEUS AFTER INDUCTION OF MICRO-SPORE EMBRYOGENESIS BY STRESS Seguí J.M., Testillano P.S., and Risueño M.C. Lack of epigenetic reprogramming in early sheep embryos produced by somatic cell nuclear transfer Beaujean N., Taylor J., Gardner J., Wilmut I., Meehan R. and Young L. MAMMALIAN RRN3 BECOMES INACTIVATED IN THE PROCESS OF TRANSCRIPTION Hirschler-Laszkiewicz, I., Cavanaugh, A., and Rothblum, L. ACTIVE MAPKs TRANSLOCATE TO THE NUCLEUS AND ARE TARGETED TO DEFINED DOMAINS DURING PLANT DIFFERENTIA-TION AND PROLIFERATION Coronado M.J., Seguí J.M., González-Melendi P., Ramírez C., Barany, I., Testillano P.S., and Risueño M.C. Enhancement of nuclear uptake of therapeutic genes and macro-molecules by using NLS and PTD containing viral peptides Sanders N, Vandenbroucke R, De Smedt S, Vanderleyden E, Lucas B, Remaut K Demeester J evidence that The nuclear lamina is an elastic structure which exerts pressure on intranuclear macro-molecules Hancock R. POSTER SESSION page 34
September 6^th 8.30- 13 am Collegio Nuovo September 6^th 8.30- 13 am Collegio Nuovo	NUCLEOLUS page 18 UBF INDUCES CHROMATIN REMODELING Chen D., Belmont A. S. and Huang S. ARTIFICIALLY UNRAVELED NUCLEOLI AS A MODEL TO STUDY THE NUCLEOLUS FUNCTIONAL ORGANIZATION Dudnik O.A. and Smirnova O.Iu. EFFECT OF ACTINOMYCIN-D UPON UP-STREAM BINDING FACTOR LOCALIZATION: A (3D + TIME) STUDY Elias E., Tchélidzé P., Benassarou A., Sauvage C., Bobichon H., Ploton D. and O’Donohue M.F. CONNECTION OF NUCLEOLAR TRANS-CRIPTION AND PROCESSING MACHINERY IS ENERGY-DEPENDENT Louvet E., Junéra H.R., Le Panse S. and Hernandez-Verdun D. TOPOGRAPHY OF RIBOSOMAL DNA (rDNA) DETECTABLE BY FISH IN EARLY MOUSE EMBRYOS Korobova F.V., Noniashvili E.M., Schoefer C., Romanova L.G., Wachtler F., Dyban A.P., and Zatsepina O.V. Proteomic and functional analysIs of the Arabidopsis nucleolus Shaw P, Beven A, Clark G, Lam Y, Andersen J, Mann M, Lamond A , Brown J FUNCTIONAL PROTEOMIC ANALYSIS OF PLANT NUCLEOLAR PHOSPHOPROTEINS RELATED TO CELL PROLIFERATION González-Camacho, F., and Medina, F.J. TRAFFIC OF NEWLY FORMED RIBOSOMES IN THE NUCLEUS Politz J.C., Tuft R.A. and Pederson, T. RECRUITMENT OF RIBOSOMAL PROTEINS TO PRE-RIBOSOMES AS STUDIED BY EXPRESSION OF GFP-FUSION PROTEINS IN LIVING CELLS Krüger, T., and Scheer, U. LOCALIZATION OF COMPONENTS OF THE SIGNAL RECOGNITION PARTICLE IN THE AMPLIFIED NUCLEOLI OF XENOPUS OOCYTES Sommerville J., Mullin A., Gilchrist K., Lewandowski L.B., Brumwell C.L., Politz J.C. and Pederson T. ROLE OF THE NUCLEOLUS IN HUMAN REPRODUCTION Kittur N., Bazett-Jones D.P.and Meier U.T.
September 7^th 8.30- 11 am Collegio Volta 11.30 Collegio Volta	CELL DEATH & NUCLEAR MARKERS OF DISEASE page 23 FURTHER CONSIDERATIONS ON APOPTOTIC CELL NUCLEAR DOMAINS. Burattini S., Luchetti F., Battistelli M. and Falcieri E. NUCLEAR CHANGES IN BREAST CANCER CELLS DURING EARLY PHASES OF CELL DEATH AND CELL GROWTH. Losa G.A., Castelli C. and Nonnenmacher T.F. MODELING APOPTOTIC CHROMATIN CONDENSATION IN NUCLEI ISOLATED FROM NORMAL CELLS Widlak P. SYNCHRONIZED ONSET OF NUCLEAR AND SURFACE CHANGES DURING APOPTOSIS OF HUMAN, RAT AND MOUSE LYMPHOCYTES Dini L. and Abbro L. FURTHER NOTES ON NUCLEI AND NUCLEOLI IN THE PROGRAMMED CELL DEATH AND PRECEDING TERMINAL MATURATION Smetana K., Čermák J., and Hrkal Z. AGGRESOME-LIKE INCLUSIONS WITHIN THE NUCLEOPLASM: PROTEASOMAL DEGRADATION AND SUB-NUCLEAR PATHOLOGY Chen M., Rockel T., Hemmerich P., and von Mikecz A. MOLECULAR CHARACTERIZATION AND DEVELOPMENTAL EXPRESSION OF THE XENOPUS EMERIN Gareiß M., Zentgraf H., Wilken N., Müller C.R. and Dabauvalle M.-C. WILHELM BERNHARD JUNIOR LECTURE page 26 NUCLEAR MYOSIN I AND ACTIN ARE REQUIRED FOR RNA POLYMERASE I TRANSCRIPTION Philimonenko V.V., Iben S., Dingová H., Kyselá K., Grummt I., de Lanerolle P. and Hozák P.
Sptember 7^th 2.30-4 pm Collegio Volta Sptember 7^th 2.30-4 pm Collegio Volta	CELL CYCLE page 27 50 YEARS NOBEL PRIZE FRITS ZERNIKE IN PHASE CONTRAST MICROSCOPY (1953 – 2003) Gundllach H. DELAYED ENTRY TO QUIESCENCE BY p21^CDKN1A-NULL HUMAN FIBROBLASTS: A POSSIBLE INVOLVEMENT IN THE DISASSEMBLY OF PRE-REPLICATION COMPLEX Stivala L.A., Perucca P., Cazzalini O., E. Prosperi, Madine M. and Laskey R.A.L. LIGHT AND ELECTRON MICROSCOPIC STUDY OF REPLICATION SITES IN HELA CELLS DURING S-PHASE Koberna K., Pliss A., Siegel A.J., Malinsky J., Ligasova A., Dudnik O. Berezney R., and Raska I. REPLICATION FACTORIES AND INTRA-S PHASE CHECKPOINT PATHWAYS Rossi R., Biamonti G and Montecucco A. PERSISTENT DNA DECATENATIONS BETWEEN SISTER CHROMATIDS INDUCE CHECKPOINT DELAYS IN G2 AND EARLY MITOSIS De la Torre C, Moreno Díaz de la Espina S, Clarke DJ and Giménez-Abián JF.
September 8^th 8.30-10.30 am Collegio Nuovo 11-12.30 am	NUCLEAR PROTEINS, MATRIX AND BODIES page 31 DEVELOPMENTAL CHANGES IN THE DISTRIBUTION OF TIGHT DNA-PROTEIN COMPLEXES ALONG BARLEY CHROMOSOMES REVEALED BY MAPPED MOLECULAR MARKERS Sjakste T. and Röder M. Polymorphism of nuclear multi-functional protein XPD and its influence on DNA repair Rzeszowska-Wolny J., Pietrowska M., Polanska J., Palyvoda O., Jaworska J., Butkiewicz D. and Hancock R. NUCLEAR STRESS BODIES REVEAL A LINK BETWEEN CHROMATIN ORGANIZATION AND DISTRIBUTION OF RNA PROCESSING FACTORS. Biamonti G., Rizzi N., Chiodi I., Corioni M., Denegri M., Cobianchi F., Riva S. SIGNAL SEQUENCES IN THE MOLECULE OF THE NUCLEOLAR PROTEIN FIBRILLARIN DIRECTING ITS SPECIFIC LOCALIZATION WITHIN THE NUCLEOLUS ^1,3Mukharyamova K.Sh., ^2Levitsky S.A., ^2Veiko V.P., and ^1,3Zatsepina O.V. MOLECULAR ARCHITECTURE OF THE SYNAPTONEMAL COMPLEX: SCP3 PROTEIN DOMAINS THAT ARE ESSENTIAL FOR POLYMERIZATION Baier, A., Alsheimer, M., and Benavente, R. CHROMATIN page 29 DYNAMICS OF NUCLEAR COMPARTMENTS DURING EARLY EMBRYOGENESIS IN THE MOUSE Martin C., Zatsepina O., Migné C., Chebrout M., Beaujean N., Zink D. and Debey P. HISTONE EXPRESSION IN THE GERM LINE OF DROSOPHILA Hennig W., Becker A., Feng RR, Berger, A., Ye J., Tang X. MALE CHROMATIN REMODELING AFTER FERTILIZATION IN SEA URCHINS: THE ROLE OFA THIOL Concha C, Morín V., Even Y., Oliver M.I., Gutiérrez S., Puchi M., Genevière A.M., and Imschenetzky M. EXPRESSION OF HUMAN METHYLASES IN THE MALE GERM LINE OF DROSOPHILA Weyrich A., Dong M., Ge Y., Xu G., W. Hennig DYNAMICS OF HETEROCHROMATIN PROTEINS MEASURED BY FLUORESCENCE CORRELATION SPECTROSCOPY IN LIVING CELLS Schmiedeberg L., Weißhart K. and Hemmerich P. THE NON-HISTONE CHROMOSOMAL PROTEIN HMGB1 AS A MODULATOR OF CHROMATIN STRUCTURE Polyanichko A., Chikhirzhina E., Skvortsov A., Kostyleva E., Vorob’ev V.

THE WILHELM BERNHARD LECTURE

Ultrastructural analysis is a major tool in investigating the nuclear functional architecture

Stan Fakan

Centre de Microscopie Electronique

Université de Lausanne, Switzerland

ORAL PRESENTATIONS

TRANSCRIPTION AND SPLICING