COURSE CODE: 7MBO1C3 CORE COURSE - III – MICROBIOLOGY AND PLANT PATHOLOGY Course Objectives
To understand about the structure, biology and mode of nutrition of virus and bacteria.
To provide knowledge on pathogens causing diseases in plants and their mode of action.
To provide information on Host parasite interaction.
MICROBIOLOGY Unit – I
Five kingdom system by Whittaker (1969) – Prokaryotic and Eukaryotic microbes – General features of Viruses – Classification, characteristics, ultra structure, isolation, purification, chemical nature – replication and transmission, Virions and prions, phytoplasma (including mycoplasma), Economic importance.
Unit – II
Bergey’s system of Bacterial classification (1984-1991) – Salient features of Eubacteria, Archibacteria, Cyanobacteria and Actinomycetes. General account on Bacteria, ultra structure, nutrition, growth, reproduction (transformation, conjugation, transduction and sex-duction), bacterial culture technique and economic importance.
PLANT PATHOLOGY Unit - III
Plant pathology – general account on organisms and causal factor responsible for plant diseases – methods of studying plant diseases – Koch’s postulates – common terminologies used in plant pathology (symptomology, Etiology, Epidemic disease, Control measures) – Mycotoxins – Aflatoxins, Defense mechanisms in plant – integrated disease management.
Unit – IV
Common plant diseases of India -Tobacco Mosaic, Cucumber mosaic, Little leaf disease of Brinjal, Citrus canker, Rice blight, Tikka disease of groundnut, Anthracnose of mango, Wilt of Cotton, Downy mildew of grapes, White rust of Mustard, Damping off disease of seedlings, Rust of wheat, Root knot of tomato, Leaf galls in Pongamia.
Unit – V
Host parasite interaction: Recognition and entry processes of different pathogens like bacteria, viruses into animal and plant host cells, alteration of host cell behavior by pathogens, virus-induced cell transformation, pathogen-induced diseases in animals and plants, cell-cell fusion in both normal and abnormal cells.
Power and Daginwala (1994). General Microbiology. Himalayan Publishing House,
Pelczar, M. J., Chan, E. C. S. and Krieg, N. R. (1993). Microbiology. Tata McGraw Hill Publishing Co. Ltd., New Delhi.
Mehrota, R. S. (1994). Plant Pathology. Tata McGraw Hill Publishing Co. Ltd., Delhi.
Pandey, B. P. (1982). A Textbook of Plant Pathology, Pathogen and Plant Diseases.
S.Chand and Co. Ltd., New Delhi.
Books for Reference: Microbiology
Dubey, R. C. and Maheshwari, D. K. (2007). A Textbook of Microbiology. S. Chand and Co. Ltd., New Delhi.
Sharma, P. D. (1992). Microbiology. Rastogi & Co., Meerut.
Staley, J. T. et al.. (1991). Bergey’s Manual of Systematic Bacteriology. Vol. I to IV. Williams & Wilkins, London.
Davis, B. D., Dulbecco, R., Eiser, H. N. and Grinsberg, H. S. (1980). Microbiology. Harper & Row, New York.
Carpenter, P. L. (1967). Microbiology. Saunders Co., Philadelphia, USA.
Cooper, J. I. (1995). Viruses and the Environment. 2nd ed. Chapman & Hall, London.
Bilgrami, K. S. and Dube, H. C. (1990). A Textbook of Modern Plant Pathology. Vikas Publishing House Pvt. Ltd., New Delhi.
Singh, R. S. (1990). Plant Diseases. 6th ed., Oxford & IBH, New Delhi.
Rangaswamy, G. and Soumini Rajagopalan. (1973). Bacterial Plant Pathology. Tamil Nadu Agricultural University, Coimbatore.
Rangaswamy, G.(1972) Diseases of Crop Plants in India. Prentice Hall of India P Ltd.
Southey, J. F. (1965). Plant Nematology. Tech. Bull. No.7, Ministry of Agricultural, Fisheries and Food, Her Majesty’s Stationery Office, London.
Smith, K. M. (1957). A Textbook of Plant Virus Diseases. Little Borwn & Co., Boston.
Walker, J. C. (1952). Diseases of Vegetable Crops. McGraw Hill Book Co. Inc., NY
Butler, E. J. and Jones, S. G. (1949). Plant Pathology. Macmillan & Co., London.
I YEAR – I SEMESTER
COURSE CODE: 7MBO1C4 CORE COURSE - IV – CELL BIOLOGY AND BIOPHYSICAL CHEMISTRY Course Objectives
To study and impart knowledge about Cellular Organization.
To impart knowledge about molecules and their interaction relavent to biology.
Unit - I
Membrane structure and function (Structure of model membrane, lipid bilayer and membrane protein diffusion, osmosis, ion channels, active transport, membrane pumps, mechanism of sorting and regulation of intracellular transport, electrical properties of membranes). Structural organization and function of intracellular organelles (Cell wall, nucleus, mitochondria, Golgi bodies, lysosomes, endoplasmic reticulum, peroxisomes, plastids, vacuoles, chloroplast, structure & function of cytoskeleton and its role in motility).
Unit - II
Organization of genes and chromosomes (Operon, unique and repetitive DNA, interrupted genes, gene families, structure of chromatin and chromosomes, heterochromatin, euchromatin, transposons). Cell division and cell cycle (Mitosis and meiosis, their regulation, steps in cell cycle, regulation and control of cell cycle). Microbial Physiology (Growth yield and characteristics, strategies of cell division, stress response)
Unit - III
Structure of atoms, molecules and chemical bonds. Composition, structure and function of biomolecules (carbohydrates, lipids, proteins, nucleic acids and vitamins). Stabilizing interactions (Van der Waals, electrostatic, hydrogen bonding, hydrophobic interaction, etc.). Metabolism of carbohydrates, lipids, amino acids, nucleotides and vitamins.
Unit - IV
Principles of biophysical chemistry (pH, buffer, reaction kinetics, thermodynamics, colligative properties). Bioenergetics, glycolysis, oxidative phosphorylation, coupled reaction, group transfer, biological energy transducers. Principles of catalysis, enzymes and enzyme kinetics, enzyme regulation, mechanism of enzyme catalysis, isozymes
Unit - V
Conformation of proteins (Ramachandran plot, secondary structure, domains, motif and folds). Conformation of nucleic acids (helix (A, B, Z), t-RNA, micro-RNA).Stability of proteins and nucleic acids.
Jain J.L. 2016 Fundamentals of Biochemistry S.Chand & Co., New Delhi.
Conn E.E. and Stump – Outlines of Biochemistry. Wiley Eastern Ltd. Chennai.
Gymnosperms – Lyginopteris, Lagenostoma and Cordaites.
CORE COURSE II METHODS IN BOTANY Experiments
Measure the cell size (either length or breadth/diameter) by micrometry.
Determination of leaf thickness to width ratio
Field study (Local and outside)
Floristic survey, Documentation and evaluation of plants (Ranking of species, Relative importance of species (RI), Importance Value(IVs), Quality Use Value (QUVs) and Cultural Importance Index (CI).
Study of Morphological parameters (Plant height, root and shoot length, root/shoot ratio, index of tolerance, root and branch number, internode and petiole length, Leaf number, Leaf length, Leaf width, Leaf area, petiole leaf ratio, Leaf area index and leaf appearance characteristics)
Physiological parameters (Dust deposition, EC, pH, RWC, Ash content and Air pollution tolerance index of plants).
Preparation of Temporary and Permanent slides
Preparation of Temporary and Permanent Whole Mount
Analysis of ash sample
Camera lucida drawing of permanent slide
Flow cytometry (FACS) Applications of fluorescence microscopy:
Transmission and Scanning electron microscopy
Core course IIIMicrobiology:
Gram’s staining of Bacteria found in Milk, Curd, Root – Nodule.
Microbial analysis of Milk by methylene – blue reduction test
Preparation of culture media agar slant – agar plate.
Isolation of microbes by streak and pour plate method.
Isolation of microbes by soil dilution techniques.
Isolation and identification of Bacteria and Fungi from spoiled food.
Effect of different antibodies on bacterial growth (antibiotic sensitivity)
Cleaning and sterilization methods
Laminar air flow chamber
Leaf gall T.S of Pongamia
Cercospora infected leaf T.S
Study of the following diseases :
Fungal diseases – Rust of Wheat, Wilt of cotton, White rust of mustard
Bacterial diseases – Anthracnose of mango, Citrus canker, Rice blight.
Virus diseases – Tobacco mosaic, cucumber mosaic.
Mycoplasmal disease – little leaf of brinjal.
Core course IV CELL BIOLOGY ANDBIOPHYSICAL CHEMISTRY CELL BIOLOGY Experiments
Determination of cell stages in onion root tip and anther
Micropreparation of cystolith(Ficus leaf), Raphides(Araceae members eg. Arum petiole) and starch grains(Potato and Rice grains)
Cell division and cell cycle
Structure of proteins
Types of DNA (helix (A, B, Z), t-RNA, micro-RNA)
BIOPHYSICAL CHEMISTRY Experiments
Estimation of total and reducing sugar
Estimation of protein by Lowry’s method.
Estimation of amino acids by ninhydrin method
Estimation of total phenols.
Estimation of proline.
Estimation of Nucleic acids.
Separation of photosynthetic pigments by paper chromatography.
Separation of photosynthetic pigments by Column chromatography
Separation and identification of amino acids by paper/TLC method
Extraction of amylase and determination of its activity
Determination of peroxidase activity.
Duly certified record note should be submitted for all the practical examinations and those who do not submit the records need not be permitted to the concerned practical examination.
FIELD STUDY/ TOUR : The students should undertake as part of their course an institutional visit and a field study of Botanical methods under the guidance of the staff for not less than FIVE DAYS with in the state and neighbouring state. Students who have not undertake the above activities shall forfeit the appropriated marks for this purpose (05 marks) for the practical examination.
For CC - V – 50 marks for experiments, 04 marks for Field study and Report and 05 marks for Record.
CORE COURSE-V – PRACTICAL QUESTION 1. Microprepare the given specimens A,B, C & D. Stain and mount in Glycerin. Draw
labeled sketches and identify with reasons. Submit the slides for valuation.
(Micropreparation -1, Identification -1/2, Diagram -1/2, Reason -1) (4x3 =12 Marks) 2. Identify any two microscopic algal types in the given algal mixture E.
To study the principles and practices of Mushroom cultivation.
To provide skills in Mushroom cultivation.
Unit – I
Introduction, history. Nutritional and medicinal value of edible mushrooms; Poisonous mushrooms. Types of edible mushrooms available in India - Volvariella volvacea, Pleurotus citrinopileatus, Agaricus bisporus.
Unit – II
Cultivation Technology : Infrastructure: substrates (locally available) Polythene bag, vessels, Inoculation hook, inoculation loop, low cost stove, sieves, culture rack, mushroom unit (Thatched house) water sprayer, tray, small polythene bag. Pure culture: Medium, sterilization, preparation of spawn, multiplication. Mushroom bed preparation - paddy straw, sugarcane trash, maize straw, banana leaves. Factors affecting the mushroom bed preparation - Low cost technology, Composting technology in mushroom production.
Unit - III
Cultivation techniques of White button mushroom and Oyster mushroom; Management of fungal, bacterial and viral diseases in mushroom; Competitors, pests and nematodes in mushrooms; Post harvesting techniques and Economics of mushroom cultivation.
Unit – IV
Storage and nutrition : Short-term storage (Refrigeration - upto 24 hours) Long term Storage (canning, pickels, papads), drying, storage in saltsolutions. Nutrition - Proteins - amino acids, mineral elements nutrition - Carbohydrates, Crude fibre content - Vitamins.
Unit – V
Food Preparation_: Types of foods prepared from mushroom. Research Centres - National level and Regional level._Cost benefit ratio - Marketing in India and abroad, Export Value. Present status of mushroom industry in India.
Tripathi, D. P. (2005). Mushroom Cultivation. Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi.
Nita Bhal. (2000). Handbook on Mushrooms. 2nd ed. Vol. I and II. Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi.
Alice, D., Muthusamy and Yesuraja, M. (1999). Mushroom Culture. Agricultural College, Research Institute Publications, Madurai.
Pathak, V. N. and Yadav, N. (1998). Mushroom Production and Processing Technology. Agrobios, Jodhpur.
Marimuthu, T. et al. (1991). Oster Mushroom. Department of Plant Pathology. Tamil Nadu Agricultural University, Coimbatore.
Marimuthu, T. Krishnamoorthy, A.S. Sivaprakasam, K. and Jayarajan. R (1991) Oyster Mushrooms, Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore.
Swaminathan, M. (1990) Food and Nutrition. Bappco, The Bangalore Printing and Publishing Co. Ltd., No. 88, Mysore Road, Bangalore - 560018.
To study the history and importance of Ethnobotany.
To learn techniques related to Ethnobotanical studies.
To understand the application aspects of Ethnobotany.
Unit - 1 Ethnobotany: Introduction, concept, scope and objectives; Ethnobotany as an interdisciplinary science. The relevance of ethnobotany in the present context; Major and minor ethnic groups or Tribals of India, and their life styles. Plants used by the tribals: a) Food plants b) intoxicants and beverages c) Resins and oils and miscellaneous uses.
Unit – II Methodology of Ethnobotanical studies: a) Field work b) Herbarium c) Ancient Literature d) Archaeological findings e) temples and
Unit - III Role of ethnobotany in modern Medicine: Medico-ethnobotanical sources in India;Significance of the following plants in ethnobotanical practices (along with their habitat and morphology) a) Azadiractha indica b) Ocimum sanctum c) Vitex negundo. d) Gloriosa superba e) Tribulus terrestris f) Pongamia pinnata g) Cassia auriculata h) Indigofera tinctoria. Role of ethnobotany in modern medicine with special example Rauvolfia sepentina, Trichopus zeylanicus, Artemisia, Withania.