Curriculum Vitae of Dr. M. Balasubramanyam Assistant Director & Senior Scientist
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Curriculum Vitae of Dr.M.Balasubramanyam Dr.M.Balasubramanyam Assistant Director & Senior Scientist Madras Diabetes Research Foundation (MDRF) Gopalapuarm, Chennai – 600 086, India. Email: balusignal@gmail.com Qualifications : MSc., M.Phil., PhD., MNASc. Specialization: Cell Signaling in health and disease & Molecular Medicine with special reference to molecular mechanisms of pathogenesis of diabetes and its vascular complications; Insulin signaling; Role of Proteomics, Epigenetics & miRNA aspects in diabetes research; Unraveling mechanisms of hyperglycemic memory; Redox signaling, Oxidative stress and biomarkers identification; Bioprospecting herbal molecules. Positions held in chronological order: 2007 to till date: Assistant Director & Senior Scientist, Madras Diabetes Research Foundation, Chennai, India 1999 to August 2007: Senior Scientist, Madras Diabetes Research Foundation, Chennai 1996-1999: Faculty, Centre for Biotechnology, Anna University, Chennai 1991-1996: Post Doctoral Fellow, American Heart Association Affiliate, New Jersey Medical School, New Jersey, USA 1988-1991: Research Associate (CSIR), S.V.University, Tirupati 1986-1988: Senior Research Fellow (CSIR), S.V.University, Tirupati 1984-1986: Junior Research Fellow, S.V.University, Tirupati. Additional Responsibilities: 1999-till date: Member, Institutional Ethics Committee, Madras Diabetes Research Foundation 1999-till date: Academic Coordinator, MDRF (Training programmes for BSc/BTech; MSc/MTech students; short-term exposure training and end-semester project training) 2008-till date: Member Secretary, Institutional Animal Ethics Committee, MDRF Research Experience: Possessing > 25 years of post-MSc research experience since 1983. This includes an extensive Post Doctoral Fellow (PDF) training program (1991-95) at the University of Medicine and Dentistry of New Jersey (UMDNJ), New Jersey Medical School, New Jersey, USA, on the subject: “Biochemical machinery and Signal transduction in physiology and pathophysiology”. Training at the University of San Diego, USA (2004) on human skeletal muscle/adipose biopsy and insulin signaling profiling. Teaching experience: 25 years of total teaching experience which include teaching assignments at a) Department of Zoology, S.V.University, Tirupati (1983-1991), b) Centre of Biotechnology, Anna University, Chennai (1995-1999) c) MDRF (1999 -till-to-date). Research Grants: 1993-1995 American Heart Association Fellowship grant (PI) 1996-1997 Third World Academy of Sciences (TWAS), Italy - Research Grant (PI) 2002-2005 Department of Science & Technology (DST), New Delhi (PI) 2002-2007 FIST-DST Grant (Programme Coordinator) 2003-2006 Department of Biotechnology (DBT) (Co-Investigator) 2006-2010 Indian Council of Medical Research (ICMR) (Co-Investigator) 2003-2006 Department of Science & Technology (DST), New Delhi (Co-PI). 2007-2012 Department of Biotechnology (DBT), New Delhi (PI). 2009-2012 Department of Science & Technology, Indo-Korea Joint Research Project (PI) PhD students under my supervision at the Cell and Molecular Biology Department of MDRF (affiliated to the University of Madras) completed/working on the following topics:
Summer training & End-semster project training to students: So far, >200 summer students trained and above 35 project students were directly supervised and guided for their end-semster project work. Nominated & Served as PhD examiner for students outside the MDRF: Conducted PhD viva-voce examination and served as examiner for students from: University of Madras Tuberculosis Research Centre, Department of Biotechnology, CLRI Central University Hyderabad National Institute of Pharmaceutical Education and Research (NIPER) Post-Graduate Institute of Medical Education & Research (PGIMER) Centre for Biotechnology, Anna University Professional Recognition(s): (i) Served as Guest-Editor for a special issue on Diabetes Mellitus in “Current Science” (2002, Vol: 83(12). (ii) Co-organizer of the meetings organized by MDRF including a) Brain Storming session on “Cell transplantation” sponsored by Department of Science & Technology (DST), Govt of India, 2002 at MDRF, Chennai Scientific Organising Secretary: for b) MDRF-ADA Post-graduate Course in Diabetology 2002 c) MDRF –ADA Post-graduate Course in Diabetology 2004 d) MDRF –ADA Post-graduate Course in Diabetology 2006 (iii) Organising Secretary – Indo-Swedish Symposium on Genomics and Proteomics of Diabetes (April 1-2, 2006) (iv) Served as Guest-Editor for a special issue on Diabetes Research in “Indian Journal of Medical Research” (Vol 125) 2007. Referee for various journals including: Molecular and Cellular Biochemistry Indian Journal of Experimental Biology Current Science Indian Journal of Medical Research European Journal of Biology Diabetes Technology & Therapeutics BMC Endorcine disorders DRCP, JCMM, IJEB, Diabetes Care, Metabolism Scientific Services: Member - Task Force committee on Cardio-vascular diseases (CVD) in Department of Biotechnology (DBT), Govt of India Member – Expert Committee on Clinical Proteomics, Department of Biotechnology, Govt. of India Member – Life Sciences Research Board (LSRB) under DRDO, New Delhi. Member – Board of Studies, Biotechnology, Loyola College, Chennai Editorial Board Member: Diabetes Technology & Therapeutics Memberships & Research Awards:
Membership of Academies/Research Societies: Asia-Pacific International Molecular Biology Network (A-IMBN) Member- Madras Science Foundation (MSF) Member Indian Biophysics Society (IBS) Life member Indian Association of Biomedical Scientists (IABMS) Life member Indian Society of Cell Biology (ISCB) Life member-European Calcium Society (ECS, Germany) Member Alliance for Cell Signaling (AFCS, USA) Member Society of Biological Chemists (India) (SBC) Member Indian Science Congress Association (ISCA) Member Inter Academy of Biomedical Forum – (IABF) Research publications in accredited journals:
Talks delivered (2005-2010) by Dr.M.Balasubramanyam Asst. Director & Senior Scientist, MDRF“Oxidative stress in diabetes – Multiple cell stressors and challenges” International Conference on Recent Advances in Free radical Research, Natural Products, Antioxidants and Radioprotectors in Health and 9th Annual Conference of Society for Free Radical Research-India (SFRR-India)” Hyderabad, January 11-13, 2010. “Stress Wars” in Diabetes – Molecular Insights – Guest Lecture at the Division of Metabolic Diseases, Korea National Institute of Health (KNIH), 21st December 2009, South Korea “Accelerated Senescence in Diabesity? New Molecular Insights”. International Conference on Nutrition and Physical Activity in Aging, Obesity and Cancer, 12-15th December 2009, Jeju Island, South Korea. “Role of endoplasmic reticulum (ER) stress in the molecular pathogenesis of insulin resistance and Type 2 diabetes”. International Symposium on “Type 2 diabetes: From molecular insights to therapeutic intervention” Hyderabad, India; September 18-20, 2009. “Mitochondrial dysfunction and ER stress in diabetes”. 37th Annual Meeting of RSSDI, Ahmedabad, 5-8th November 2009. “Basic methods in Eukaryotic Cell Culture” Guest Lecture given at the AICTE-STTP Programme on Animal Tissue Culture in Indian Institute of Technology (IIT), Chennai 21st July 2009 “Palmitate causes insulin resistance by inducing epigenetic alterations in hepatocytes and adipocytes” - 13th ADNAT Convention, International Symposium on Epigenetic Modifications of the Genome: Mechanisms and Implications, at the Centre for Cellular and Molecular Biology (CCMB), Hyderabad during February 23-24, 2009. “Molecular pathogenesis of Type 2 diabetes: Technological struggle and the Gordian Knot” – MDRF-ICMR Workshop on Techniques in Genomics in relation to diabetes and related disorders, MDRF, Chennai, 9th January 2009 “Molecular intricacies of oxytosis and apoptosis in Type 2 diabetes” – 77th Annual Meeting of the Society of Biological Chemists, Indian Institute of Technology, Chennai, 18-20 December 2008. “Prevention is better than cure – Molecular perspective on how this ancient adage is appropriate for diabetes” – State level Symposium on Pre-emptive prevention of human diseases, Loyola College, Chennai, 18th December 2008. “Phytochemicals for diabetes – Time tested or Time to Test?”- International Conference Natural Products in Health" and "21st Annual Conference of the Indian Society for Atherosclerosis Research", Annamalai University, Chidambaram, 8th December 2008. “Functional foods and their molecular mechanisms in relation to diabetes”. 36th Annual meeting of the Research Society for the Study of Diabetes in India (RSSDI), Hyderabad, 23rd November 2008. “Stem cells for diabetes – Where do we stand now?” First International Stem Cell Summit India -2008, Indian Institute of Technology (IIT), Chennai, 14th November 2008. “Oxidative damage and altered HO-1 signaling in Type 2 diabetes” at the Soongsan Biomedical Symposium, Wonkwang University, Korea, 13th October 2008. “Traditional medicine & Diabetes: New molecular insights” at the Workshop on “Opportunities for collaboration through e-IMBL, 11th annual meeting of the A-IMBN Conference, Seoul Korea, 8-9th October 2008. “New insights of translational research opportunities in diabetes”. 11th annual meeting of the A-IMBN Conference, Seoul, Korea, 8-9th October 2008. “Stress Wars” in Diabetes: Patholgoical insights and new therapeutic concepts”. International Congress on Cell Biology, Seoul, Korea, 7-10th October 2008. “Harnessing the benefits of Biotechnology for better diabetes care”. Talk delivered at the Kumararani Meena Muthiah College of Arts and Science, Chennai on 12th September 2008. “Vision care in diabetes: Visualizing beyond glucose control”. KNBIRVO Conference, Chennai, 5th September 2008. “Phytomedicine & Diabetes: New molecular insights” 1st International Euro-India Conference on Holistic Medicine (ICHM-2008), Institute of Holistic Medical Sciences, Kottayam, 21-23 August, 2008. “Diabetic retinopathy: seeing beyond glucose control” 56th TNOA Conference:Research and Ophthalmology/Organizing Research at the Regional Ophthalmic Institute and Govt Ophthalmic Hospital, Chennai, 9th August, 2008. Participated at the International discussion meeting on “Cell replacement therapy for diabetes” (DBT/NCCS), National Centre for Cell Science, Pune, 15-16 July 2008. “Diabetes – in search of an integrated management and interdisciplinary research” at the International Biomedical Modeling School and Workshop, National Centre for Biological Sciences (NCBS), Bangalore, Feb 27- March 2, 2008. “Recent trends in Biotechnology and Healthcare with special reference to diabetes”. Talk by Video Conference, Biotechcellence-2008. Center for Biotechnology, Chennai, 25th February 2008 “Stress wars in diabetes” – role of oxidative stress and endoplasmic reticulum (ER) stress – at the 95th Indian Science Congress, Visakhapatnam, 3-7th January 2008. “Stem Cell Therapy: applications, obstacles and opportunities” – Plenary talk delivered at the State Level Seminar on Emerging Operandum in Stem Cell Biology & Nanotechnology – Chennai, 29th December 2007. “Molecular divergence of O-linked N-acetylglucosaminyl transferase (OGT) silencing, ER stress and Insulin resistance in L6 skeletal muscle cells” – Indo-French Conference on: RNAi in Genome Control, Centre for Cellular and Molecular Biology (CCMB), Hyderabad, December 12 -14, 2007. “Stem cells, diabetes and regenerative medicine” – Frontier Conclave 2007 – International Conference on Stem Cell, Tissue Engineering and Regenerative Medicine. Frontier Lifeline, Chennai, November 30th – December 2nd 2007. “Metabolic Memory and Oxidative Stress – New Insights From Interventional and Epidemiological Studies” talk at the 35th Annual Conference of RSSDI, Kolkatta, 23-25th November 2007 “Molecular understanding of Type 2 diabetes – Triumphs and Trials” talk at the Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR), Bangalore, 12th November 2007. “Exploring novel mechanisms of insulin resistance and Type 2 diabetes using miRNA and RNAi technologies”- presentation at the Department of Biotechnology (DBT), Govt. of India, 26th September 2007. “Multiple diabetes drug targets – A blessing in disguise for Pharma” invited talk given at the National Institute of Pharmaceutical Education & Research (NIPER), Chandigarh, 11th August 2007. “Research needs – Chronic Disease Biology & Diabetes”. Department of Biotechnology (DBT) meeting on Chronic Disease Biology, Trivandrum 7-9th August 2007 “Insulin resistance and Type 2 diabetes: New insights in to the molecular mechanisms” Guest Lecture at the Department of Biotechnology, School of Life Sciences, Central University, Hyderabad on 30th July 2007. “Oxidative stress, Metabolic memory and Diabetic retinopathy” at the 16th Annual Meeting of Indian Eye Research Group (IERG), L.V.Prasad Eye Institute, Hyderabad during 28 –29th July 2007 “Molecular pathogenesis of Type 2 diabetes-Cell and Organellar Insights” at the School of Life Sciences, MDIS, Singapore on 3rd July 2007 Methods in Calcium homeostasis and Redox signaling at the School of Life Sciences, MDIS, Singapore on 29th June 2007 “Pathogenesis of Type 2 diabetes – Technology struggles and the Gordian Knot” at the Department of Biological Sciences, National University of Singapore, Singapore on 3rd July 2007. “Molecular pathogenesis of Type 2 diabetes – Tissues, Issues and Microarray applications” at the “Workshop on advanced microarray techniques”, Genotypic Technology, Bangalore, 3 - 4th May 2007. “Type 2 diabetes – Emerging drug targets and exciting challenges” Talk given at BIOCON, Bangalore on 28th March 2007. “Stem cells, diabetes and bioengineering enterprise”, Guest lecture on Tissue Engineering at the Department of Biotechnology, University of Madras, Chennai, 6th March, 2007. “Molecular pathogenesis of Type 2 diabetes-Cell and Organellar Insights” at the 30th All India Cell Biology Conference (AICBC), Theme: Molecules to Compartments; Corss-talks and Networks. Delhi University, Delhi, 2-4 February, 2007. Diabetes – What medicinal plants can offer? at the National Conference on “Recent Trends in Medicinal Plants Research”, University of Madras, Chennai, 24-25 January, 2007. “Phytochemicals and fight against diabetes mellitus” at the XXIII Annual conference of the Indian Association of Veterinary Pathologists, TamilNadu, Veterinary and Animal Sciences University, Chennai, 27th December 2006. “Telomere shortening at the cross-road of oxidative stress and atherosclerosis” at the 19th Annual Conference of the Indian Society for Atherosclerosis Research (ISARCON-2006), University of Madras, Chennai, December 16-17th , 2006 “Herbal medicine and diabetes mellitus: Where do we stand now?” at the Natioanal symposium on Current trends in the development of herbal drugs & 27th Annual Conference of Indian Association of Biomedical Scientists, Trivandrum 25-27th November, 2006. “Screening techniques on herbals for insulin secretagogue and insulin sensitizing actions” at the IABMS (Indian Association of Biomedical Scientists) Workshop on “Basic tools and techniques in herbal drug research”, Trivandrum 25-27th November, 2006 “Changing trends and translational approaches in studying the molecular pathogenesis of Type 2 diabetes” at the 9th A-IMBN (Asia-Pacific International Molecular Biology Network) Conference & 16th MSMBB Scientific Meeting, Theme: From Genomics and Proteomics to Translational Initiatives, held in Kuala Lumpur, Malaysia during 3rd to 5th September 2006. “Does oxidation/inflammation impose accelerated cellular senescence in adipocytes and modify their secretory profile and function?” – Presentation at the Department of Biotechnlogy (DBT), Govt. of India, 28th August 2006. “War against diabetes mellitus: Phytochemicals revisited?” at the AVT Institute of Advanced Research (AVTAR), Coimbatore on 7th July 2006 “Proteomics and post-translational modifications-Type 2 diabetes context” at the Indo-Swedish Symposium on Genomics and Proteomics of Diabetes held in Chennai during April 1-2, 2006 “Changing trends in studying the molecular pathogenesis of diabetes” at the National Seminar on Molecular basis of diseases – current perspectives and new frontiers, held at the University of Madras, Chennai during 13-14 March 2006 “Time for a translational approach in diabetes research” at the Seminar on ‘Emerging trends in biomedical sciences and their translational applications’ at the Loyola College, Chennai, 1st March 2006. “New insights into the pathogenesis of diabetes and the role of phytochemicals” at the Workshop cum Symposium on “Recent trends in Biochemistry & Biotechnology” at the Annamalai University, Chidambaram, on 26th February, 2006. “Differential regulation of Ca2+ & Endoplasmic reticulum (ER) stress markers during induction of insulin resistance by glucosamine in L6 muscle cells”, at the10th ADNAT convention on: “RNAi & microRNA in development and health”, Center for Cellular & Molecular Biology (CCMB), Hyderabad, during 23-24th February, 2006. “Insulin signaling, diabetes pathogenesis and proteomics aspects” at the National Symposium & Workshop on Genomics, proteomics and computational biology – Today and Tomorrow, in Chennai during 15-17 February, 2006. “Antioxidatns & Metabolic/Vascular diseases – Where do we stand now” at the Joint Annual Meeting of the International Society of Heart Research & International Academy of Cardiovascular Sciences, held at the Madras Medical Mission, Chennai during 12-14th January, 2006 “Oxidative stress and diabetes – New insights for therapeutic intervention” at the 38th Annual Conference of the Indian Pharmacological Society (IPS), Chennai, 29th December 2005. “Diabetes & New Horizons in Drug Development” at the National Conference on “Path to Health – Biotechnology Revolution in India” held at the Anna University, Chennai, 25th November 2005. “Clinical Proteomics and diabetes: Expectations and Emerging Opportunities” at the Expert Committee Meeting of the Department of Biotechnology (DBT) held in Post Graduate Institute of Medical Education & Research (PGIMER), Chandigarh, 7th November 2005. “Herbal medicine, diabetes and oxidative stress as a therapeutic target” at the “International Conference on Drug Development from Medicinal Plants- Issues and Problems” held at CLRI, Chennai on 21st October 2005. Brief Profile: Dr.M.Balasubramanyam is Assistant Director & Senior Scientist at the Cell and Molecular Biology department of the Madras Diabetes Research Foundation (MDRF). He is a member of the Asia-Pacific International Molecular Biology Network (A-IMBN) and had an extensive post-doctoral training (1991-95) at the Hypertension Research Centre, New Jersey Medical School, USA, in the areas of signal transduction in health and metabolic diseases with special reference to molecular pathogenesis of Type 2 diabetes and vascular biology. Having worked as a faculty at the Center for Biotechnology, Anna University, Chennai for 3 years (1996-99) he has joined MDRF in 1999. He is instrumental in setting up the basic infrastructural facilities at MDRF and coordinates various research programmes and educational activities from time-to-time. Mission & Vision: Dr.Balu’s laboratory is involved in studying cell and molecular signatures with special reference to insulin secretion, insulin action and vascular function. Our research integrates physiological and pharmacological approaches with biochemical, molecular biological and proteomics techniques to study mechanisms underlying development and progression of diabetes, its complications and cardiovascular diseases. This challenging multi-disciplinary research specialization dwells around the following themes:
The department now serves as an excellent 'interface' for biomedical/biotechnology/biopharmaceutical work with translational applications. The goal of the department is set to evolve as a ‘Centre for Cellular and Molecular Medicine’ (CCMM) with state-of-the-art facilities for preclinical research, biomarker(s) identification, high-throughput cell-based assay systems, and to offer world-class contract research and consultancy work in the areas of specialization. Research Highlights One of the thrust areas of research at the Madras Diabetes Research Foundation (MDRF) is to use the biochemical and proteomic technologies for identifying novel biomarkers and drug targets in the development and progression of diabetes and its associated complications. In the sub-set of subjects from CURES, we have shown elevated oxidative reactions (Sampathkumar et al 2005) and increased advanced glycation end products (AGEs) (Sampathkumar et al 2004) in patients with Type 2 diabetes and its vascular complications. Related to oxidative damage, our pilot observations indicated that there was increased lipid and protein oxidation as early in the stage of impaired glucose tolerance (prediabetes). In addition, we have seen telomere shortening in prediabetes subjects and patients with Type 2 diabetes (Adaikalakoteswari et al 2005; Adaikalakoteswari et al 2007), arising either from a cumulative burden of oxidative stress and/or other phosphorylation or epigenetic post-translational mechanisms. Another study (Adaikalakoteswari et al 2007) has demonstrated an association of oxidative DNA damage with PARP and NFkB activation, indicating a role for poly (ADP)-ribosylation in the pathogenesis of Type 2 diabetes. Moreover, a role for oxidative stress in diabetes patients was also substantiated with differential expression of NADPH oxidase and hemoxygenase gene (Adaikalakoteswari et al 2006). While these effects have been studied by classic biochemical and molecular biology methods, the recent onset of proteomics methods are expected to allow studying oxidative stress responses on a much wider scale with more emphasis and focus on identification of biomarkers. In this direction, in collaboration with Indian Institute of Science, Bangalore, we have recently identified increased positivity for glutathionylated hemoglobin (HbSSG) as a specific biomarker in diabetic patients with microangiopathy (Sampathkumar et al 2005b). As a prerequisite to study the proteomics & genomics of Type 2 diabetes, we have standardized in-house, the culturing of human skeletal muscle cells (HSMCs). Since insulin resistance of skeletal muscle primarily results from impaired glucose uptake and glycogen synthesis, these two metabolic read-out assays were first standardized in HSMCs (Balasubramanyam, 2006). The fact that defects in these metabolic read-outs are persisted in culture conditions of HSMCs obtained from patients with Type 2 diabetes, indicates that these cells are an excellent model system to study the proteomics & genomics of Type 2 diabetes. From the clinical proteomics view, our pilot studies also indicated protein spots with region-specific differences in plasma samples of subjects exhibiting varying degrees glucose tolerance. Other interesting studies which yielded preliminary data include: convergence of ER stress and insulin resistance in L6 skeletal muscle cells, palmitate-inudced impairment of multimerization of adiponectin in 3T3 adipocytes, characterization of a novel PTP inhibiting activity in prodigiosin, etc. As a part of the NMITLI diabetes project on herbal medicine, both curcumin and gallic acid have been extensively studied and scientifically documented for their specific molecular actions. Gallic acid has been shown to possess novel (hitherto unknown) actions at the interface of transcription factors, membrane transport, down regulation of oxidants and upregulation of antioxidant enzymes etc. Specialized training: Dr.M.Balasubramanyam is a member of the Asia-Pacific International Molecular Biology Network (A-IMBN) and had an extensive post-doctoral training (1991-95) at the Hypertension Research Centre, New Jersey Medical School, USA, in the areas of signal transduction in health and metabolic diseases with special reference to molecular pathogenesis of Type 2 diabetes and vascular biology. Under the Scientific Investigator training programme at AIIMS, New delhi, he has learned both basic and advanced applications of Electron Microscopy techniques. He is well-versed with transport assays, fluorescence imaging techniques and flowcytometry applications. In the year 2003, he has visited the University of San Diego and learned techniques related to human skeletal muscle and adipose tissue culturing, differentiation protocols, and he is well-versed with the insulin signaling assays such as, glucose uptake, lipolysis, tyrosine phosphorylation, PTP inhibition, glycogen synthase activity, lipid accumulation, palmitate oxidation etc. He has participated in several training programmes of frontier technologies including microarray, siRNA, 2-Dgel electrophoresis and mass spectrometry and his team is actively collaborating with Indian Institute of Science (Prof.P.Balaram & Dr.Utpal Tatu) on body fluid and tissue proteomics profiling and JNCASR (Dr.Tapas Kundu) on epigenetic analysis. He is instrumental in setting up the basic science infrastructure in MDRF and with his efforts the tissue culture facility at MDRF is now fully tuned to conduct research on target-specific cells such as human skeletal muscle cells, human retinal endothelial cells, 3T3-L1 adipocytes, L6 muscle cells, THP-1 monocytes and -cells. He is also instrumental in establishing the animal laboratory at MDRF. The training offered at this department is considered unique in that it is tailor-made for the students of biotechnology and pharmaceutical research. Yüklə 75,45 Kb. Dostları ilə paylaş:
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