Designation : Assistant Professor Address



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Dr. Sugapriya. D

Mobile No: 00966-532094216

E-mail: sughaphd@gmail.com   

1. Designation : Assistant Professor
2. Address : Department of Medical Laboratory Sciences

(Haematology/Microbiology),

College of Applied Medical Sciences,

Salmon Bin Abdulaziz University,

Wadi Ad Dawasir, Riyadh,

Kingdom of Saudi Arabia.


3. Phone Number’s : 00966-532094216
4. E-mail : sughaphd@gmail.com
5. Date of Birth : 03.09.1977
5. Education (Highest Degree):
Degree : Ph. D. (2008)

University : University of Madras, India



Specialization : Cancer Biology

Thesis Title : Biochemical, cellular and molecular studies on the pharmacological effects of Semecarpus anacardium Linn on Bcr-Abl+ experimental leukemic model with special reference to apoptosis. 

Education Qualification:

Apr 2004 - Aug 2008 Doctorate of Philosophy (Ph.D.) in Microbiology with Pathology from University of Madras, TN, India.

Jun 2001- Aug 2002 Master of Philosophy (M.Phil) in Microbiology from PRC affiliated to Bharathidasan University, TN, India, Percentage of marks 75.9 and year of passing 2002.

Jun 1998- Apr 2000 Master of Microbiology (M.Sc) from V.H.N.S.N College affiliated to Madurai Kamarajar University, TN, India, Percentage of marks 68.9 and year of passing 2000.



Jun 1995- Apr 1998 Bachelor of Microbiology (B.Sc) from Muthayammal College affiliated to University of Madras, TN, India, Percentage of marks 68.9 and year of passing 1998.

Career Summary:


  • Presently working as an Assistant Professor in Salmon Bin Abdulaziz University, Department of Medical Lab Sciences (Haematology), College of Applied Medical Sciences, Wadi Ad Dawasir, Riyadh, Kingdom of Saudi Arabia.

  • Employed as a Women Scientist by Department of Science and Technology, India at Indian Institute of Technology Madras, Chennai.

  • Recently worked on stem cell differentiation as a Women Scientist (Postdoc) in Indian Institute of Technology Madras, Chennai from Feb 2010. This work is awarded for travel grant of USD$ 1250 and registration fee in 10th Annual meeting of International Stem cell Society for Research (ISSCR) at Yokohama, Japan.

  • Employed as a Research Scholar (worked on anti-leukemic effect experimental animal) in Dr. A.L.M. Post-Graduate Institute of Basic Medical Sciences,University of Madras, Chennai.

  • Worked as a Lecturer in P-G department of Biotechnology, KRMMC, Chennai.

  • Worked as a Lecturer in the P-G Dept. of Microbiology, Valliammal college, Chennai

  • Worked as a Lecturer in U-G department of Microbiology, Bharathi College, Kallakurichi.

Awards/Honors

  • ISSCR Travel Grand Awarded by ISSCR Committee (USD $1250 and $600 for Registration Fee).

  • Conference Travel grant awarded by Department of Science and Technology, Govt. of India, India).

  • Indian National Science Academy (INSA) Travel grant awarded by INSA Academy Govt. of India, India.

  • CICS Award by CICS Oraganisation by Govt. of India, India.

  • Paper Presentation has been selected and wins the Third prize in ICSCC-2010 conference.

  • Selected in Women scientist (WOS-A) (Post doctorate) DST scheme from Feb-2010 to Feb-2013.  

  • Selected as University Research Fellow from April 2006-April 2008.  

  • Selected as Project Associate from April 2004 to March 2006.  

  • D.Sugapriya, P.Sachdanandam and P.Shanthi. Effect of Poly (Leucine – Glutamic acid) on oxidative stress related toxicity in human lymphocytes. Abstract book , P.123, International Conference on antioxidants and free radicals in Health- Nutrition & radio-protectors” and V International Conference of the society for free radical Research in India(SFRR), 16th-18th January, 2006. (Selected for Participate in Young Scientist Award).

  • Gold Medal in my M.SC., degree with Rs. 3000/- Cash award in 2000.

Journal Editorship/ Editorial Members


  • Editorial Team Member of International Journal of Immunotherapy and Cancer Research.

Research Projects:

S.No

Title

Sponsoring Agency

Value

Status

1.

Targeting Therapeutic Nanoparticles coated Recombinant Vasostatin as a Propective Strategy to combat cancer by Modulating Vasculature.

Kingdom of Abdulaziz City of Science and Technology (KACST) – Saudi Arabia. March 2015

5.05 L SAR

Commences in September 2015

2.

Induction of functional hepatocytes-like cells from mouse mesenchymal stem cells by FOXA-2 for liver development.

Department of Biotechnology (DBT) – India.

23 L INR

Completed

ACADEMIC RESEARCH AND TEACHING APPOINTMENTS:

Institute/College

Designation

Role

Duration


Department of Medical Lab Sciences (Haematology), College of Applied Medical Sciences, Salmon Bin Abdulaziz University, Wadi Ad Dawasir, Riyadh, Kingdom of Saudi Arabia.

Assistant Professor

Course coordinator: Clinical Haematology I, Clinical Haematology II, Clinical Microbiological Practice and Immunology

Sep 2014 – Till Date

Indian Institute of Technology Madras, Chennai

Women Scientist *


Research in Stem Cell Biology

Feb 2010 – Feb 2013

Indian Institute of Technology Madras, Chennai

Post Doctoral Fellow

Research in Cancer Cell Biology

Aug 2008 – Jan 2009


University of Madras

Research Scholar

Research in Leukemia (Cancer Biology – in in vitro and in vivo model)

Aug 2008 – Jan 2009


KRMMC (Affiliated with University of Madras)

Lecturer in P-G Dept of Biotechnology

Course coordinator: Molecular Biology, Animal biotechnology, General microbiology, practical for molecular biology and practical for animal biotechnology.


Jun 2003 – April 2004

Valliammal College (Affiliated with University of Madras)

Lecturer in P-G Dept of Biotechnology

Course coordinator: Medical microbiology, Bacteriology and practical for Bacteriology


Sep 2002 – Mar 2003

Bharathi College (Affiliated with University of Madras)

Lecturer in U-G Dept of Biotechnology

Course coordination: Medical microbiology, Molecular biology, Immunology and practical for molecular biology and immunology.

Aug 2000 – Mar 2001

* This work is awarded for travel grant of USD$ 1250 and registration fee (USD$ 600) in 10th International Stem cell Society for Research (ISSCR) at Yokohama, Japan.



INTERNATIONAL REFEREED JOURNALS (STARTING FROM RECENT)
  1. Sugapriya Dhanasekaran, Devilakshmi Sithambaram, Kavitha Govarthananan, Bijesh Biswal and Rama Verma. An Efficient Protocol for Deriving Liver Stem Cells from Neonatal Mice: Validating Its Differentiation Potential. Analytical and Cellular Pathology (In Press).

  2. Shanthi Palanivelu, Sugapriya Dhanasekaran, Haseena Banu Hedayathullah Khan, Sachdanandam Panchanadham. Antileukemic effect of Kalpaamruthaa, a modified Siddha preparation, in BCR–ABL+ cell line induced chronic myeloid leukemic mouse model. Comparative Clinical Pathology 23: 653-664, 2014.


  3. Sugapriya D, Bijesh K.Biswal, Venil.N.Sumantran and Rama S Verma. Augmented Sensitivity to Methotrexate by Curcumin induced overexpression of Folate Receptor in KG-1 Cells. Biochimie 95: 1567-1573, 2013.
  4. Sugapriya Dhanasekaran, Ravindran Jaganathan, Sachdanandam Panchanadham and Shanthi Palanivelu. Induction of Mitochondrial Mediated Apoptosis by Semecarpus anacardium in the BCR-ABL+ 12B1 Leukemia cell line – a possible mechanism of therapeutic action in vivo. Journal of Experimental and Clinical Medicine ; 4(1):30-38 2012.

  5. D.Sugapriya, S.Preethi, P.Shanthi, N.Chandra, G.Jeyaraman, P.Sachdanandam, S.Thilagavathy, S.Venkatadesilalu. BCR-ABL translocation in pediatric acute lymphoblastic leukemia in Southern India. Indian Journal of Hematology and Blood Transfusion.  Volume 28: 1, 37-41 2012.

  6. M. Himabindu, D. Sugapriya Muthamilselvan, Dillip Kumar Bishi and Rama S. Verma Molecular Analysis of Coagulase Gene Polymorphism in Clinical Isolates of Methicilin Resistant Staphylococcus aureus by Restriction Fragment Length Polymorphism Based Genotyping. American Journal of Infectious Diseases 5 (2): 170-176, 2009.

  7. Sugapriya Dhanasekaran, Shanthi Palanivelu and Sachdanandam Panchanadham. Restorative effect of energy metabolism in leukemic mice treated by a Siddha drug - Semecarpus anacardium Linn. nut milk extract. Chemico Biological Interaction 173: 1,9, 43-58, 2008.

  8. Balamurugan.S, Sugapriya.D, Shanthi.P, Thilaka, Venkatadesikalu.S, Pushpa.V, and Madhavan.M. Multidrug resistance 1 (mdr1) gene expression and Agnors in childhood acute leukemias. Indian Journal of Hematology and Blood Transfusion. 23: 3-4, 73-78, 2007.

Book Published

  1. Rama S Verma, Abhilash, Sugapriya M.D and Chithra R. Animal Biotechnology: Applications and Potential Risks. In: Biotechnology in Functional Foods and Nutraceuticals. First editon. Ed Debasis Bagchi, Francis C. Lau, Dilip K. Ghosh. CRC Press, Taylor &. Francis Group, (Boca Raton London NewYork) pp 219-250.

Paper Communicated

  1. Sugapriya D, Devilakhsmi S and Rama S Verma. Induction of functional hepatocytes-like cells from mouse mesenchymal stem cells by FOXA-2 for liver development. (Journal of Hepatology)

Poster / Oral Presentation(S) In Conference

  1. Sugapriya D, P.Shanthi and P. Sachdanandam. Emerging insights into antioxidant-directed therapy by Semecarpus anacardium nut extract (SA) in BCR-ABL+ cell line induced chronic myeloid leukemic mouse model. 34th Annual Conference of Indian Association of Biomedical Scientists will be held at Dr. ALM. PGIBMS, University of Madras, Tharamani Campus, Chennai - 600 113. Oral presentation wins the First prize.

  2. Sugapriya D and Rama S Verma. Induction of functional hepatocytes-like cells from mouse mesenchymal stem cells by FOXA-2 for liver development. International Society for Stem Cell Research (ISSCR), 10th Annual Meeting on June 13-16, 2012 held at Pacifico Yokohama, Japan. Presentation wins Travel Grand Award USD $1250 and Registration Fee (USD $600). (Dept. of Science and Technology selected for travel grant).

  3. Sugapriya D, Prassana V, Rajalakshmi S, Rama S Verma. Increased levels of folate receptor in KG-1 cells by curcumin augmented sensitivity to methotrexate. 3rd International Conference on Molecular Medicine on February 20-23, 2012 held at VIT, Vellore.

  4. Sugapriya DM, Sreejit P and Rama S Verma. Development of a simplified protocol for isolating liver derived hepatocyte stem cell from neonatal mice at International conference on Stem Cells and Cancer (ICSCC-2010): Proliferation, Differentiation and Apoptosis on December 11-14, 2010 held at International Institute of Information Technology, Pune. Poster presentation wins the Third prize

  5. Sugapriya D Muthamilselvan, P.Shanthi and P. Sachdanandam Mechanism of induction of apoptosis in 12B1 leukemic cells by Semecarpus anacardium Linn. nut milk extract at International Conference on Molecular Medicine on 18th – 20th January 2009, held at Indian Institute of Technology Madras, Chennai.

  6. P.Shanthi, Sugapriya D Muthamilselvan and P. Sachdanandam Recuperative efficacy of Kalpaamruthaa (KA), a Siddha preparation on altered antioxidant status in experimental leukemia at International Conference on Molecular Medicine on 18th – 20th January 2009, held at Indian Institute of Technology Madras, Chennai.

  7. M. Himabindu, D. Sugapriya Muthamilselvan, Dillip Kumar Bishi and Rama S. Verma Molecular Analysis of Coagulase Gene Polymorphism in Clinical Isolates of Methicilin Resistant Staphylococcus aureus by Restriction Fragment Length Polymorphism Based Genotyping at International Conference on Molecular Medicine on 18th – 20th January 2009, held at Indian Institute of Technology Madras, Chennai.

  8. Rama S Verma, Bijesh K. Biswal. Abhilasha, Chithra R and Sugapriya D. M. (2009) Innovation and Challenges in Biotechnology (ICICB-08) ISBN: 978-93-80043-16-6

  9. P.Shanthi, D.Sugapriya, S.Preethi, N.Chandra, G.Jeyaraman, P.Sachdanandam S.Thilagavathy, S.Venkatadesilalu BCR-ABL translocation in pediatric acute lymphoblastic leukemia. “APCON-2007” Annual IAPM Conference PGIMER, Chandigarh, November 26th-29th, 2007. Indian J Pathol Microbiol Vol 50, Suppl. 2007 p 44.

  10. D.Sugapriya, P.Sachdanandam and P.Shanthi. Therapeutic Efficacy of semecarpus anacardium Linn. Nut Milk Extract on Carbohydrate Metabolizing Enzymes in Chronic Myeloid Leukemic mice. Abstract book , P.12, ASPUM International Symposium on Science in New Millennium, 12th – 13th June, 2006.

  11. D.Sugapriya, P.Sachdanandam and P.Shanthi. Emerging role of Semecarpus anacardium Linn. nut extract on inhibitory effect of oxidative damage induced by Fe2+, Cu2+ and H2O2 in peripheral blood mononuclear cells. P.13, International Conference on Ethnopharmacology and Alternative Medicine V annual Conference of the National society of Ethnopharmacology 20th-22nd January, 2006.

  12. D.Sugapriya, P.Sachdanandam and P.Shanthi. Effect of Poly (Leucine – Glutamic acid) on oxidative stress related toxicity in human lymphocytes. Abstract book , P.123, International Conference on antioxidants and free radicals in Health- Nutrition & radio-protectors” and V International Conference of the society for free radical Research in India(SFRR), 16th-18th January, 2006. (Participate in Young Scientist Award)

  13. D.Sugapriya, P.Sachdanandam and P.Shanthi. Effect of Gallium Nitrate on Tamoxifen treated breast cancer related hypercalcemia with reference to calcium and magnesium in rats. Abstract book , P.129, International Conference on antioxidants and free radicals in Health- Nutrition & radio-protectors” and IV annual Conference of the society for free radical Research in India(SFRR), 10th-12th January, 2005.

RESEARCH INTEREST

Project # 1


IIT Madras, Chennai, India Feb 2010 – Feb 2013

Project: Induction of Functional Hepatocyte-Like Cells from Mouse Mesenchymal Stem Cells by FOXA-2 for Liver Development

Short Abstract: Induced hepatocyte-like cells have multiple hepatocyte-specific features and aids in reconstituting damaged hepatic tissues after transplantation. To differentiate mouse mesenchymal stem cells into functional hepatic cells using FOXA-2 a master regulator of liver-specific gene expression for liver development. In this study, we examined the role of FOXA-2 in hepatic differentiation from mouse MSCs, which were efficiently generated by transfection and then the expression of hepatic markers of the hepatocyte-like cells were assessed. Outcome: We have developed a robust and efficient method to differentiate mesenchymal stem cells into hepatic like cells, which exhibits characteristics of mouse hepatocytes. Our approach would facilitate the development of hepatocytes for liver engineering and regenerative medicine.

Responsibilities:

  • Isolation, Culture, Expansion and Characterization of MSCs from mouse bone marrow.

  • Cloning, Transfection, G418 Selection, in vitro Hepatogenic Differentiation.

  • Hepatic Markers expression confirmed by Cytological, Molecular and Biochemical analysis.

Experimental platform:

Stem Cell, Cloning of gene interest, Immunocytochemistry, Multilineage differentiation, RNA isolation, Real-Time PCR, Western Blot, PAS (Glycogen storage), Biochemical analysis.


Project # 2


IIT Madras, Chennai, India Feb 2010 – Feb 2013

Project : Development of a Simplified Protocol for Isolation and Characterization of Liver-Derived Stem Cell (LDSCs) from Neonatal Mice

Short Abstract: The ability to isolate and expand liver-derived stem cells (LdSCs) is an important step in the development of tissue engineering approaches for liver repair or regeneration for the treatment of liver damage, as well as for their application in plastic or reconstructive surgery. Here, we describe step-by-step procedures on the basis of frequent medium change in primary culture and diminishing the trypsinization time. In this protocol, we have shortened the enzyme digestion period and LdSCs enrichment step. Outcome: The present study describes an improved method for rapid isolation of LDSCs from neonatal mice, as well as the maintenance and propagation of such cultures for the long term. After isolation and culture, these cells behave similarly to those in vivo, including their ability to proliferate, providing an ideal system for the study of hepatic stem cell proliferation and multilineage differentiation. The successful isolation and cultivation of LdSCs will allow to study their unique biological properties which have almost unlimited proliferation capabilities while retaining the potential to differentiate in vitro into various progenitor used for therapeutic approaches.

Responsibilities:

  • Isolation, Culture and Expansionof LDSCs from neonatal mouse.

  • Characterization of LDSCs by immunophenotyping and Immunocytochemistry

  • Further Characterized by Multilineage Differentiation and Molecular analysis.

Experimental platform:

Liver derived stem cell, Immunophenotyping, Immunocytochemistry, Multilineag differentiation, RNA isolation, RT- PCR.


Project # 3


IIT Madras, Chennai, India Aug 2008 – Jan 2009

Project: Augmented Sensitivity to Methotrexate by Curcumin induced overexpression of Folate Receptor in KG-1 Cells

Short Abstract: Folate receptors are targets of various strategies aimed at efficient delivery of anti-cancer drugs. Therefore, it is important to identify agents which increase expression of folate receptors in cancer cells. The present study aimed to investigate the role of curcumin in augmenting expression of folate receptors in leukemic tumor cells. Outcome: Our results strongly suggest that curcumin’s ability to enhance the cytotoxicity of methotreate in KG-1 leukemic cells, occurs via up-regulation of expression of folate receptor β protein and enhancement of methotreate transport. We hypothesize that administering optimal concentration of curcumin in combination with low-dose of methotreate, could be a promising strategy for treatment of leukemia. This strategy would potentially reduce the genotoxic effects associated with methotreate treatment

Responsibilities:

  • Revival and Maintenance of KG-1 cells.

  • Standardization of curcumin treatment along with and without methotreate.

  • Analysis of folate receptor expression

Experimental platform:

Cancer cell line, Immunocytochemistry, Real-Time PCR, Western blot, Transport mechanism by liquid santillation Counter.


Project # 4


University of Madras, Chennai, India April 2004 – April 2008

Ph.D. Thesis

Topic: Biochemical, cellular and molecular studies on the pharmacological effects of Semecarpus anacardium Linn on Bcr-Abl+ experimental leukemic model with special reference to apoptosis

Summary of the Thesis:

Chronic Myeloid Leukemia (CML) is a malignant clonal disorder of haematopoietic stem cells leading to massive expansion of myeloid lineage cells at all stages of maturation and development. CML is associated with a specific chromosomal translocation known as the “Philadelphia (Ph) chromosome” t (9; 22) (q34; q11), which is a BCR-ABL translocation; this translocation encodes for the BCR-ABL fusion protein, which is a constitutively active tyrosine kinase. A Tyrosine kinase inhibitor, Imatinib (Gleevac) which specifically inhibits BCR-ABL is being used in the chronic phase and in blast crisis. However, imatinib also has side effects. Moreover, the innumerable reports in literature of patients developing resistance to imatinib are of great concern. Thus the search for newer, more effective antileukemic drugs with fewer side effects continues.



Semecarpus anacardium Linn.nut milk extract (SA) has already been shown to have anticancer activity in experimental breast cancer and hepatocellular carcinoma. Using the 12B1 BCR-ABL+ murine cell line (in vitro) and a murine model (in vivo) of BCR-ABL+ leukemia, we have evaluated SA for its antileukemic potential, its possible mode of action via the apoptotic pathway and restorative efficacy on the biochemical and apoptotic changes observed in the disease condition.

The study included assessment of altered marker and lysosomal enzymes, antioxidant and non antioxidant levels at serum, liver and spleen level, altered in energy metabolism, apoptotic bodies (TEM) study, macromolecular damages like single strand DNA breaks and DNA-protein cross links (in vivo). Drug induced apoptosis was studied in vitro by using 12B1 leukemic cell line. Propidium iodide, DNA fragmentation, and protein and mRNA expression of Bcl-2, Bax, Cytochrome c, p53, caspase-3 and 9 by western blot and RT- PCR and the mRNA expression of BCR-ABL was studied by RT-PCR to confirm that the SA induced apoptosis through mitochondrial mediated pathway and also inhibit the BCR-ABL mRNA. Our study concludes the chemotherapeutic action of SA and suggested it as a major therapeutic value against leukemia.



Responsibilities:

  • Antioxidant and antimutagenic property of Semecarpus anacardium in cell free system.

  • Effects of Semecarpus anacardium on the biochemical and cellular functions (Serum/Plasma, Spleen and Liver) in leukemic experimental animals.

  • Mitochondrial alteration and energy metabolism of Semecarpus anacardium in leukemic experimental animals.

  • Analysis of in vitro apoptosis (12B1 cell line) by Semecarpus anacardium

Experimental platform:

Induction of leukemia in experimental animal, Biochemical enzymatic analysis, TEM analysis, Histopathology, RT-PCR, apoptosis analysis, Western blot.


Project # 5


PR College, Bharathidasan University

CLRI, Chennai Jan 2002 – Aug 2002

M.Phil Project: Perturbation and Dysfunction of Intracellular Ca2+ Homeostasis in Mitochondria by Novel Amyloid Forming Model Peptide Poly (Leucine-Glutamic Acid)

Short Abstract: Amyloid forming model peptide Poly (Leucine-Glutamic acid) (LE) toxicity on peripheral blood mononuclear Cells (PBMC) are mediated by oxidative stress inducing apoptosis and macromolecular damage with depletion of GSH, leads to decreases PBMC cell viability. Cell death was prevented by the antioxidant vitamin E shows to be effective in reducing the accumulation of reactive oxygen species (ROS) in cells exposed to poly (LE). Enhanced ROS increases oxidized protein concentration and the leakage of Ca2+ ions from the mitochondria and finally leads to significant increase of intracellular calcium. Based on the above findings, we postulate that poly (LE) fibril directly involves in the disruption of mitochondrial function, contribute to the deficiency of energy metabolism and increased calcium homeostasis leads to DNA fragmentation. Outcome: In conclusion, our studies demonstrate that oxidative stress of poly (LE) fibril toxicity leads to mitochondrial dysfunction responsible for spontaneous generation of intracellular ROS. Present study suggests that vitamin E could be used for prevents the formation of amyloid plaques and we hypothesized that vitamin E may also have some role in reducing the fibril formation of poly (LE) in neurodegenerative diseases such as alzheimer’s and in self-assembly of peptides.

Responsibilities:

  • Isolation and maintenance of Peripheral Blood Mononuclear Cells (PBMC) from blood.

  • Measurement of Cytotoxicity

  • Detection of apoptosis

  • Prevention of amyloid plaques formation by vitamin E.

Experimental platform:

MTT assay, Measurement of intracellular ROS, Analysis of mitochondrial membrane potential, Intracellular calcium levels, Quantification of endogenous GSH, DNA fragmentation.


Project # 6


V.H.N.S.N. College,

Madurai Kamarajar University, Nov 1999 – April 2000

M.Sc Project: Production of Streptomycin and compare the individual and combined effect with penicillin for various organisms. 

Short Abstract: Production and purification of antibiotic from rhizospere isolates and compare the individual and combined effect for various organisms. Isolation of various organisms from rhizospere region for production of streptomycin and penicillin antibiotic. Selection and characterization of strains produces more antibiotic from the isolates. Production and purification of these antibiotics by column chromatography. Compare these antibiotic effects by individual and combination with various organisms. Outcome: In our study the yield of Streptomycin and penicillin production from Rhizosphere isolates is equal to that of ATTC culture. When compare with the individual effects the combined effect is highly sensitive to the penicillin resistant organisms.

Responsibilities:

  • Collection of soil from various rhizosphere regions.

  • Isolation and characterization of antibiotic producing strains.

  • Selection of strains, production and purification of antibiotics.

  • Study the individual and combined effects for various pathogenic organisms.

Experimental platform:

Column Chromatography, Antibiotic sensitivity assay.


Project # 7


Muthayammal College,

University of Madras, Nov 1997 – Mar 1998

B.Sc Project:   Effect of antimicrobial activity on Coleus aromaticus against gram negative organisms.

Short Abstract: Study the effect antimicrobial activity of Coleus aromaticus against gram negative enterobacteriaceae. Collection of Coleus aromaticus leaf and stem dried separately in shades. Prepare water extract and methanol extract separately used for further analysis. Enterobacteriaceae organisms were used for the study of antimicrobial assay for methanol and water extract. Outcome: In our study the leaf extract is highly effective against the Enterobacteriaceae when compare with stem extract. Methanol extract is less effective than water extract. Therapeutic efficacy of Coleus aromaticus is effective to Shigella when compare all other Enterobacteriaceae.

Responsibilities:

  • Collection of stem and leaf of Coleus aromaticus

  • Preparation of methanol and water extract from both.

  • Determination of the efficacy of these extract against enterobaceriaceae.

Experimental platform:

Extract preparation, Antibiotic sensitivity assay.


Technical / Functional (Application) Skills



Skills

Details



Molecular Biology and Protein Chemistry Techniques

Plasmid and genomic DNA isolation, Isolation and purification of proteins, PCR, RT-PCR, Real-Time PCR, Gene cloning and screening of recombinant clones, Southern hybridization, Electroporation, Transfection, Western Blotting, Electrophoresis – PAGE and Agarose.





Tissue Culture Techniques

Isolation, culturing, maintenance of Mesenchymal stem cell and Hematopoietic stem cell from Bone marrow (Human & Mouse) and UCB, Culturing of stem cells in microgravity, Tissue culture and maintenance of cell lines, Cell freezing and cell revival, Primary cell culture, Cytotoxicity assays, Clonogenic assay, Flowcyometry and Cell Sorting, Immunocytochemistry analysis.





Microbiological techniques

Culturing of Microbes, Microbiological techniques, Identification and culturing of pathogenic microbes





Cytochemistry, Histopathology and Handling of Animals.

Special staining for Leukemia, Immuno-cytochemistry, Immuno-histochemistry, In-situ Hybridization, Disease model, Handling of Experimental animals.





Technical Knowledge

DNA sequencing, RFLP, Polyclonal Antibody production, Microarry, Confocal Laser Scanning Microscopy, 2D and Scintillation counter.




Leadership Experience

Over the past eight years I had the blessing to be a small youth group leader to junior project students during my Ph.D. (4 Nos) and in my Postdoctoral work (6 Nos). I meet face to face with them daily for 6 month and most of the time I just talk with them about their research interest, explaining about the research work, problem of their work, solving the problem of contamination in cell culture. During my career as lecturer I was as a mentor for 2 M.Sc students for their research project. I proceeded to tell discuss them about their work and future work plan. I once had that ended badly but affirmed how they dealt with correctly and how they should go about finishing their work strong. During my postdoctoral work I was an organizer committee member for Ist International stem cell Submit 2008 held in IIT-Madras, India. For my postdoctoral work I wrote a project proposal and proposal was sanctioned by Department of Science and Technology, Government of India.



Workshop Training

Animal Cell Culture” on IIT Madras QIP Short Term Training Programme 2008-09 on 20-24th July, 2009, held at Indian Institute of Technology Madras, Chennai.

Indo-UK Workshop on Designing of Doctoral Research for Ph.D Scholars held on 23 rd March 2005 at Chennai, Tamil nadu, India.

International Seminor on Immunotechnology and workshop on immunoenzyme labeling in tissue sections, ELISA, 2D & Pulse Field Gel Electrophoresis and Fermentor held from 20th - 22nd October 2005 at Chennai, Tamil nadu, India.

IIIrd SURGICAL PATHOLOGY UPDATE held on 1st February 2008 at Chennai, Tamil nadu, India.

Certificate in Bioinformatics – Anna University, MIT Campus, Chennai, India-2005.


Summary of Teaching Experience and Pedagogy

Brief employment history

I have taught for a little over a decade as a Lecturer in private college affiliated to University of Madras..  During this time I have taught at three different Colleges, the Bharathi College of arts and Science, Valliammal College, KRMMC College.  While my appointments at KRMMC have been in their Biotechnology Departments and other institutions were as in Microbiology Department. I have also taught in programs related to my research such as Molecular Biology, Animal biotechnology, General microbiology, practical for molecular biology and practical for animal biotechnology in KRMMC College; In Valliammal College I have taught Medical microbiology, Bacteriology and practical for Bacteriology and in Bharathi college I have taught Medical microbiology, Molecular biology, Immunology and practical for molecular biology and immunology. I design my courses with three goals: 1) introduce students to major scholars and themes in the field as it relates to the specific course topic; 2) further develop students’ confidence as intellectuals and thinkers; and 3) further develop students’ practical skills and increase their employability. 

Of course, proper assessment of these and all other activities must be undertaken to ensure that the desired educational goals are being met. In designing assessment tools (notably exams, term papers and lab reports), I again attempt to challenge students to reach beyond the mere facts and to demonstrate critical thinking and a deeper understanding of the subject matter. For this purpose, I utilize predominantly essay and long answer exams, especially in the upper level and graduate courses. After the exam, I provide the answers (via Moodle) before handing back the exams (or before even posting the grades) in the hopes that students will review the exam as an added reinforcement. More recently, I have invited students to use the posted answers to remember and “grade” their exam answers in advance of receiving the actual graded exam, including of details of how and where points would be taken off. With the enticement of extra credit for this assignment, most of the class took part and I believe that this was an even better reinforcement of the material. Additionally, for all written work handed in, I try to provide constructive criticism so that students are not merely earning a grade on these assignments, but can learn how to create an even better product and gaining from the assessment process.

My experiences to date suggest that at least at some level, I am succeeding in my undertakings as teacher. However, I am well aware that there is my room for me to evolve and grow and that I can also actively learn to be a better teacher - from my colleagues and from students’ learning experiences. I believe that my goal of becoming an outstanding teacher is a journey rather than a destination. I am excited to continue on this journey.

I declare that all information provided with this application is true to the best of my knowledge and belief,

Signature of Applicant




Sugapriya Dhanasekaran

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