Executive Summary



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Germany

galactolipid, tocopherol, phylloquinone

Biochemistry and Molecular Biology of Plant Lipids: Galactolipids, Tocopherol, Phylloquinone, Chloroplast Lipids, Phospholipids, Fatty Acids

scientific

Institute of Medical Physics and Biophysics

Medical Faculty, University of Leipzig

++49-341-9715733

04107

Leipzig

Härtelstrasse 16-18

Germany

MALDI, 31P NMR, Phospholipids, Lysophospholipids

We are using using MALDI-TOF mass spectrometry and 31P NMR spectroscopy for the evaluation of physiologicallly relevant phospholipids. Besides methodological work, we are particularly interested in - the activity determination of phospholipases and phosphoinositde kinase - the lipid / phospholipid composition of tissues and body fluids - the changes of the lipid patterns under inflammatory conditions (e.g. in rheumatoid arthritis and atherosclerosis) - the changes of the lipid pattern of cells during differentaition

scientific

Institute for Biology I, Botany

RWTH Aachen University

+49-(0)241-8026641

52074

Aachen

Worringerweg 1

Germany

plant glycerolipid isoprenoid

our research focuses on plant lipid metabolism with special interest in formation/function of glycerolipids (glycerophospholipids) and isoprenoids.

scientific

Institute for Pathology, University Hospital Áachen

RWTH-Aachen

++492418089729

52074

Aachen

Pauwelstr. 30

Germany

acyl-CoA, ceramide, liver

Functional characterisation of acyl-CoA synthetase and its products, esp. in gastrointestinal tract and liver. Cellular localisation, concentration and function of acyl-CoAs and lipids.

scientific

Kekulé Institut f. Organische Chemie und Biochemie

Universität Bonn

++49 228 735346

53121

Bonn

Gerhard-Domagk-Straße 1

Germany

Sphingolipids, Glykosphingolipids, Sphingolipidoses, Lipid transfer proteins

The research of Konrad Sandhoffs group focuses on sphingolipids and sphingolipidoses. Metabolism of sphingolipids and glycosphingolipids, its function, regulation and topology are studied in vitro at the cellular and organismic level. Sphingolipid disorders are analyzed to clarify human diseases and their molecular and cellular pathogenesis. At the biochemical level, we synthesize sphingolipid probes and analogues to analyze their molecular environment in the cells, and to characterize enzymes and sphingolipid activator proteins involved in sphingolipid metabolism and membrane digestion at lipid-water interphases. We develop lipid transfer assays for the analysis of lipid transfer between membranes by transfer proteins.

scientific

Kekulé-Institute for Organic Chemistry and Biochemistry

University Bonn

++49-228-732703

53121

Bonn

Gerhard-Domagk-Str. 1

Germany

sphingosine-1-phosphate, ceramide-1-phosphate, neurodegeneration, postoperative ileus.

A)Sphingosine-1-phosphate signaling in terminally differentiated post-mitotic neurons and its implications for the pathology of neurodegenerative disorders. B)The pro-inflammatory functions of sphingosine-1-phosphate and ceramide-1-phosphate and its implications in postoperative ileus.

scientific

Department of Gastroenterology

University Heidelberg

(+49) 6221 5638960

69120

Heidelberg

INF 410

Germany

Acyl-CoA synthetases, Lipid droplets, Rafts, inflammatory bowel disease

Acyl-CoA synthetases in fatty acid uptake Lipid droplet formation Lipid rafts in fatty acid uptake Lipid rafts in intestinal inflammation Lipid MS analysis of intestinal mucus

scientific

Insitute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics

University Hospital Leipzig

++49-341-9722407

04103

Leipzig

Liebigstraße 27

Germany

phytosterols, oxysterols, eicosanoids

Sterol lipids: sterol metabolismn and atherosclerosis. Application of LC-MS/MS for the measuerment of free and esterified phtyosterols, lanosterol and cholesterol in serum and tissue. Eicosanoids: Evaluation of a LC-MS/MS method for the measurements of eicosanoids as marker of oxidative stress and inflammation.

scientific

Institute of Biochemistry I

University of Cologne

++49-221-4783656

50931

Cologne

Joseph-Stelzmann-Str. 52

Germany

lipid droplets, biosensor technology, membrane protein trafficking, endocytosis

Our interest covers the analysis of lipid droplet associated proteins and their function in lipid turnover. Coming from the field of membrane protein trafficking we apply cell based assay based on life cell imaging and conventional confocal immunofluorescence techniques to localize proteins. We further have a long-standing expertise in the analysis of biomolecular interaction analysis using surface-plasmon-based biosensors (BIAcore 3000, T-100). We analyse protein-protein interactions as well as membrane protein interactions by this very sensitive method.

scientific

Biocenter Klein Flottbekand Botanical Garden/Plant Physiology

University of Hamburg

+49-(0)40-42816-343

D-22609

Hamburg

Ohnhorststr. 18

Germany

sphingolipid, desaturase, glycosyltransferase

Molecularbiology and biochemistry of plant and fungal lipids: Functional characterizations of sterol-, sphingolipid and glycerol lipid-modifying enzymes such as glycosyltransferases and desaturases/hydroxylases.

scientific

Institut of Nutrition

University of jena

++49-03641-949610

D-07743

Jena

Dornburger Str. 24

Germany

metabolism, CLA, omega-3, sterol

fatty acid metabolism, CLA, omega-3 LC-PUFA, sterol metabolism

scientific

Institut for Food Chemistry

University of Muenster

+49 251 83 33867

48149

Muenster

Corrensstr. 49

Germany

HPLC-MS/MS fumonisin sphingolipids food ceramide synthase

HPLC-MS/MS analysis of sphingolipids Influence of fumonisins on the sphingolipid metabolism

scientific

Leibniz-Institute of Arteriosclerosis Research

University of Münster

+49 251 8356181

48149

Münster

Domagkstr. 3

Germany

coronary heart disease, biomarkers, NMR, mass spectrometry

Research on biomarkers related to chd, stroke, Alzheimer disease risk. Specific interest in technologies: H1-NMR, MS-MS, LC-MS, GC-MS.

scientific

Leibniz-Institute of Arteriosclerosis Research

University of Münster

+49 251 8356181

48149

Münster

Domagkstr. 3

Germany

coronary heart disease, biomarkers, NMR, mass spectrometry

Research on biomarkers related to chd, stroke, Alzheimer disease risk. Specific interest in technologies: H1-NMR, MS-MS, LC-MS, GC-MS.

scientific

Department of Neurology

University of Regensburg

+49 941 9410

93051

Regensburg

Universitätsstr. 84

Germany

alpha-synuclein, Parkinson, Neurodegeneration, synaptic plasticity

membrane microdomains and alpha-synuclein interaction in synaptic vesicle dynamics and plasticity

scientific

Institute for Clinical Chemistry

University of Regensburg

++49-941-944-6201

93053

Regensburg

Franz-Josef-Strauss-Allee 11

Germany

ABC Transporter, Lipid Biomarker, Biobanking

The research of the institute is directed towards the molecular analysis of metabolic and degenerative diseases. The central role of lipids in the pathogenesis of these disorders is a the main focus of the Institute of Clinical Chemistry. Our strategy combines basic research with clinical association studies enabling a rapid transfer of newly identified candidate markers from basic science to clinical diagnostics in order to support prediction, prevention, and therapy of diseases. The clinical part involves the Regensburg Diabetes Endpoint Prediction and Prevention Study (REDEPPS), an interdisciplinary platform that enables the establishment of large sample and data banks from patients with different endpoints or co-morbidities of diabetes, such as coronary heart disease, stroke, hypertension, obesity, nephropathy, retinopathy and neuropathy. Candidate markers which are verified for their diagnostic applicability in these cohorts are derived from basic research project focusing on the regulation of lipid homeostasis and vesicular traffic as related to disease pathogenesis. In this context the group has recently identified the ATP binding cassette transporter ABCA1 as a major regulator of HDL metabolism. The function of ABCA1 in the formation of lipid microdomains and vesicular transport is subject of a current project within the Transregional Collaborative Research Centre 6031 entitled Membrane Microdomains and Their Role in Human Disease funded by the DFG. The available technologies include high throughput genotyping platforms and DNA-arrays for genetic analysis, protein analysis by MALDI-TOF and the 2D-Gel Typhoon system, multicolour flow cytometry and fluorescence imaging as well as tandem mass spectrometry and NMR spectroscopy for lipids analysis. The methodological expertise of the institute is complemented by the competence centre for fluorescent bioanalysis (KFB) a public private partnership involving various institutes of the University of Regensburg.

scientific

Department of Physiological Chemistry

University of Veterinary Medicine Hannover

++49-511-953 8780

D-30559

Hannover

Bünteweg 17

Germany

Lipid microdomains, early detergent-resistant membranes, polarized protein sorting, colitis

Membrane transport, polarized protein sorting: role of lipid microdomains/detergent-resistant membranes, Intestinal membrane topology in IBD

scientific

Laboratory of Lipid Analysis

University of Ioannina

+3026510-98367

451 10

Ioannina

Panepistemioupolis

Greece

Lung surfactant, apoptosis, inflammation

Analysis of lung surfactant lipids and their interaction with surfactant proteins Lipids as substrates for phospholipases: metabolism, structural studies, modeling Lipid modifications Lipids and signal transduction Techniques in Lipid Analysis: TLC, gas chromatography, HPLC (normal and reversed phase), Mass spectrometry (FAB, EI), Light scattering

scientific

Laboratory of Nutritional Bioactivation and Bioanalysis

Department of Biochemistry and Molecular Biology, University of Debrecen

+36 30 320 97 69

4012

Debrecen

Nagyerdei Krt. 98

Hungary

HPLC MS-MS, retinoic acid, carotenoid, PUFA metabolite

HPLC MS-MS of retinoids, carotenoids and PUFA metabolites (HETEs, HODEs, eicosanoids).

scientific

Laboratory of Molecular Stress Biology

Institute of Biochemistry, Biological Research Centre, HUng. Acad. Sci.

+36-62-432-048

H-6726

Szeged

Temesvari krt. 62.

Hungary

stress sensing, lipid molecular species, mass spectrometry, rafts

Regulation of heat shock gene expression by the physical state, lipid composition, mass spectrometry of lipids, microdomain organization of membranes in different systems, the mechanism of thermoadaptation, stress sensing and signaling, cellular thermosensors, etc.

scientific

Dept of Human Morphology and Developmental Biology

Semmelweis University

36 1 215 6924/3610

1094

Budapest

Tuzolto u. 58

Hungary

caveoae, lipid rafts

In my lab we are very much interested in caveolae, mainly the function of these special lipid raft

scientific

Dept. Biophysics and Cell Biology

University of Debrecen

++36-52-455866

4010

Debrecen

Egyetem ter 1.

Hungary

P-glycoprotein, lipid rafts, cytoskeletal anchorage

We wish to understand the relationships between catalytic activity, conformational changes and topological constraints of the ABC transporter P-glycoprotein (Pgp), both in the context of multidrug resistance (mdr) and its physiological roles, also to screen more efficient/ selective Pgp modulators. We are currently focusing on the localization of Pgp within and outside of lipid rafts, in conjunction with its conformational and catalytic characteristics. Our experimental systems include: (A) a novel flow-cytometric method to detect Pgp conformational changes upon its interactions with modulators, based on antibody competition, (B) a flow-cytometric platform for the measurement of raft-, and cytoskeleton-association of cell-surface proteins, applicable for rapid, simple, serial analysis of intermolecular associations between different cell surface proteins, (C) a simple method to completely block Pgp function using an anti-Pgp mAb, (D) a xenotransplantation system in SCID mice to study, by SPECT and PET, the effect of modulators, (E) methods for fluorescence resonance energy transfer (FRET) measurements on cell surface proteins to study inter-, and intramolecular distances. We have flow-cytometers, confocal microscope equipped with FCS, expertise in fluorescence-related methods, with special emphasis on FRET.

scientific

Sackler School of Medicine-Minerva Center

Tel Aviv University

972-3-6407842

69978

Tel Aviv

Levanon

Israel

fatty acid desaturases, nutrition,metabolic syndrome

Our interest is to understand the role of fatty acid metabolism : synthesis and desaturation specifically, in pathologies leading to the alteration of glucose and lipid homeostasis.The main emphasis is on nutritional essential fatty acids. We have developed animal and cell culture models of : hypercholesterolemia ,fatty liver and diabetes. We are employing "in vitro" and "in vivo" labelled precursors to follow up lipid synthesis, fatty acid desaturation, cholesterol , bile acid synthesis and lipid composition.We measure the enzymatic pathways involved. We utilize GC, HPLC and TLC methodologies , as well as biochemical and molecular biology determinations. Our purpose is to contribute to the understanding of the impact of nutritional fatty acids on the amelioration of lipid related diseases .

scientific

Dept of Biological Chemistry

Weizmann Institute

++972-8-9342704

76100

Rehovot

1 Herzl Street

Israel

sphingolipids, ceramide, glycolipids

For 15 years. my lab has worked on sphingolipids. We focus on sphingolipid synthesis, and also defective degradation in lysosomal storage diseases. More details can be found at: http://www.weizmann.ac.il/Biological_Chemistry/scientist/futerman/

scientific

ISOF/BioFreeRadicals

Consiglio Nazionale delle Ricerche

++39-051-639-8309

40129

Bologna

Via Piero Gobetti 101

Italy

trans lipid, radical stress, isomerization

Different aspects of the cis-trans isomerization of fatty acid residues by free radicals are developed: (a) Biomimetic studies using liposomes as a model for cell membranes and correlating the isomerization process with possible mechanisms of lipid alteration occurring in vivo. The role of different vitamins. (b) A general approach has been settled for building-up of trans lipid library with application to lipidomic researches. Analytical data on trans lipids and characterization of these lipid components in living organisms, thus contributing to the lipidomic target. This is also related to the examination of biological samples and correlation between trans lipid and radical stress. Endogenous formation of trans lipids and correlation with quality of life and various pathologies.

scientific

EPR Lipidomics

Dept Biochemistry and Molecular Biology

++39 080 5443367

70126

BARI

V. E. Orabona, 4

Italy

phospholipid bilayer, spin labeling EPR, lipoperoxidation, oxidative stress

Phospholipid bilayer structure and biochemical/biophysical properties. Spin labeling of phospholipids. EPR studies of spin labelled natural and artificial membranes (mitochondria, erythrocyte ghost, lipid vesicles, oriented planar membranes). Lipoperoxidation. Synthesis and purification of oxidized phospholipid species. EPR studies of the effects of oxidized phospholipids in model membranes and in membranes reconstituted with lipidomes from pathological tissues (animal models and humans) and from cultured pathological cell mutants. Mass spectrometry lipidomics of peroxidized phospholipids, mainly lecithins (in collaboration with the local university Chemistry Department).

scientific

Istituto Superiore di Sanità

Dept of Cell Biology and Neurosciences

++39-06-49903087



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