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00161

ROME

viale Regina Elena, 299

Italy

isoprostanes, prostaglandins, oxidative stress

isoprostanes, prostaglandins, oxidative stress

scientific

Anna Maria Vaccaro Unit of Physiopatology of genetic diseases

Istituto Superiore di Sanita

++39 06 4990 2416

00161

Roma

Viale Regina Elena 299

Italy

sphingolipidoses, saposins, glucosylceramidase, anionic phospholids+

We works on a group of genetic diseases, the sphingolipidoses, characterized by the lysosomal storage of sphingolipids due to the defective activities of sphingolipid hydrolases or of their physiological activators. In particular, we have investigated the alterations in glucosylceramide catabolism in Niemann Pick disease type C. We have shown that the storage of cholesterol in Niemann Pick type C modulates the level and subcellular localization of glucosylceramidase affecting in this way the glucosylceramide degradation (Salvioli et al., J.Biol. Chem. 279, 17674-17680, 2004). Moreover, we have shown that the glucosylceramidase N370S mutation, the most common mutation in Gaucher disease, affects the capacity of the enzyme to interact with anionic phospholipid-containing membranes and saposin C, the main physiological activators of glucosylceramidase (Salvioli et al., Biochem. J., 390, 95-105, 2005). Our work is also focused on the study of the mechanism/s of action of saposins and on their interaction with lipids, especially phospholipids. Actually we have contribute to define the mechanism of action of saposin C (Vaccaro et al.,J.Biol.Chem.,272, 16862-16867,1997, Salvioli et al., FEBS Letters, 472, 1721, 2000), of saposin D (Ciaffoni et al., J. Biol. Chem., 276, 31583-31589, 2001) and of saposin B (Ciaffoni et al., J. Lip. Res., 47, 1045-1053, 2006).

scientific

Department of Cell Biology and Oncology

Mario Negri Sud Institute

+39 0872570353

66030

Santa Maria Imbaro

Via Nazionale 8/A

Italy

phosphoinositides, glycerophosphoinositols, phospholipases, LXR-FXR nuclear receptors

Phosphoinositide metabolism and related diseases. Our expertises include: -- phosphoinositide analysis by standard HPLC, TLC, Lipid binding domain recognition -- PI kinase and phosphatase assays -- inositol phospate and glycerophosphoinositol analysis by HPLC and MS -- metabolism of the phosphoinositides and their metabolites -- Morphological approaches with reference to the LBD (IF, EM, Tomography, FRET, FRAP, Correlative light/EM) Our aim is to set and apply the lipidomic approach to the lipid profiling of cell models of different pathological conditions (Overexpressing or KO for proteins/enzymes involved in lipid metabolism and related diseases) Ongoing projects: The glycerophosphoinositols (GPIs) as modulators of the Rho-family cycle: Our studies have indicated that the GPIs, the cellular derivatives of the phosphoinositides, are modulators of actin cytoskeleton assembly through an action on the Rho GTPases (Corda et al., 2002, Mancini et al., 2003). These compounds have thus become the focus of further studies aimed at elucidating their mechanism of action (Mariggiò et al, 2006) and at exploiting them as potential leads for drug development in actin-related diseases (Wiscott-Aldrich syndrome, X-linked mental retardation due to defect in PIX, common variable immunodeficiency). Diseases due to defects in the PI phosphatases: OCRL-1, MTM1 and MTMR2 (Lowe Syndrome, Myotubular Myopathy, and Type 4B Charcot-Marie-Tooth). The aim of our project is to identify and validate the enzymes controlling the PI cycle as pharmacological targets, the activities of which can be modulated by inhibitors and/or activators to re-establish the correct balance between the PIs pools in the above-mentioned conditions in which this balance has been compromised by the lack or deregulation of the PIPtases. A similar approach is currently being undertaken in the case of the deregulation of PI 3-kinase (PI3K) pathway, which occurs in cancer, thrombotic diseases, inflammation and diabetes. Other projects involving aspects of the lipid metabolism are: -role of PLA2 in membrane traffic -mechanism of membrane fissioning mediated by the protein BARS -Regulation of lipid metabolism by LXR and FXR nuclear receptor and relevance in the development of colon cancer

scientific

Department of Cell Biology and Oncology

Mario Negri Sud Institute

+39 0872570353

66030

Santa Maria Imbaro

Via Nazionale 8/A

Italy

phosphoinositides, glycerophosphoinositols, phospholipases, LXR-FXR nuclear receptors

Phosphoinositide metabolism and related diseases. Our expertises include: -- phosphoinositide analysis by standard HPLC, TLC, Lipid binding domain recognition -- PI kinase and phosphatase assays -- inositol phospate and glycerophosphoinositol analysis by HPLC and MS -- metabolism of the phosphoinositides and their metabolites -- Morphological approaches with reference to the LBD (IF, EM, Tomography, FRET, FRAP, Correlative light/EM) Our aim is to set and apply the lipidomic approach to the lipid profiling of cell models of different pathological conditions (Overexpressing or KO for proteins/enzymes involved in lipid metabolism and related diseases) Ongoing projects: The glycerophosphoinositols (GPIs) as modulators of the Rho-family cycle: Our studies have indicated that the GPIs, the cellular derivatives of the phosphoinositides, are modulators of actin cytoskeleton assembly through an action on the Rho GTPases (Corda et al., 2002, Mancini et al., 2003). These compounds have thus become the focus of further studies aimed at elucidating their mechanism of action (Mariggiò et al, 2006) and at exploiting them as potential leads for drug development in actin-related diseases (Wiscott-Aldrich syndrome, X-linked mental retardation due to defect in PIX, common variable immunodeficiency). Diseases due to defects in the PI phosphatases: OCRL-1, MTM1 and MTMR2 (Lowe Syndrome, Myotubular Myopathy, and Type 4B Charcot-Marie-Tooth). The aim of our project is to identify and validate the enzymes controlling the PI cycle as pharmacological targets, the activities of which can be modulated by inhibitors and/or activators to re-establish the correct balance between the PIs pools in the above-mentioned conditions in which this balance has been compromised by the lack or deregulation of the PIPtases. A similar approach is currently being undertaken in the case of the deregulation of PI 3-kinase (PI3K) pathway, which occurs in cancer, thrombotic diseases, inflammation and diabetes. Other projects involving aspects of the lipid metabolism are: -role of PLA2 in membrane traffic -mechanism of membrane fissioning mediated by the protein BARS -Regulation of lipid metabolism by LXR and FXR nuclear receptor and relevance in the development of colon cancer

scientific

Biochemistry group

University Milano-Bicocca

++39-02-6448-8203

20052

Monza

Via Cadore, 48

Italy

Alzheimer, Lipid rafts, signal transduction

Lipids and neurodegenerative diseases Lipid rafts and disease Lipid rafts and signal transduction Lipid-protein interaction

scientific

Department of Experimental Medicine

University Milano-Bicocca

++39-02-6448-8203

20052

Monza

Via Cadore, 48

Italy

Alzheimer, Lipid rafts, signal transduction

Lipids and neurodegenerative diseases Lipid-protein interaction Lipid rafts and signal transduction Lipid rafts and disease

scientific

Dipartimento di Scienze Biochimiche

University of Florence

++390554598328

50143

Firenze

Viale G.B. Morgagni 50

Italy

sphingosine 1-phosphate, lipid microdomain, ceramide, ceramidase

Sphingosine 1-phosphate and its mechanism of action. Regulation of cellular sphingosine 1-phosphate content. Functional role of membrane lipid microdomains in sphingosine 1-phosphate production and signalling.

scientific

Department of Clinical and Experimental Medicine, Physiopathology, University School of Medicine

University of Perugia

++39-075-5729085

06100

Perugia

Policlinico Monteluce, via Brunamonti

Italy

intranuclear lipid, DNA-lipid, RNA-lipid

We have demonstrated that a phospholipid cellular fraction is associated with chromatin. The presence, described by histochemical and biochemical techniques, is not due to membrane contaminations. The chromatin phospholipid fraction (CPF) can be considered a chromatin minor component. The CPF differ from that of microsomes and nuclear membranes as regard composition and turnover and changes in relation to hepatocyte maturation and proliferation. The main modifications, evident in several cellular functions, regard principally phosphatidylcholine (PC) and sphingomyelin (SM) that are metabolised directly in the nuclei in a very short time with a fine modulation of the enzimatic activities, regulated by PLs. The presence of a complex of base exchange enzymes for PC synthesis, a neutral sphingomyelinase (N-SMase), a sphingomyelin-synthase (SM-synthase), a phosphatidylcholine-dependent phospholipase C (PC-PLC) and a sphingomyelin-synthase-reverse (SM-synthase-reverse), which synthesises PC from SM have been demonstrated. These enzymes differ for pH and Km optima from those present in nuclear membranes, suggesting the possible existence of different isoforms. These results strongly support the presence of a metabolic machinery in the chromatin which probably is regulated in relation to cellular function. The PC-PLC and SM-synthase enrich the intranuclear pool of diacylglicerol (DAG), whereas the N-SMase and SM-synthase-reverse enrich the intranuclear pool of ceramide. DAG and ceramide are know as second messengers which play an important role in different physiological conditions, acting as a stimulating (DAG) and inhibitory factor (ceramide) of protein kinase C (PKC) The hypothesis has been made that the lipid messenger can favour the nuclear molecular events which precede DNA synthesis in liver regeneration. The results show that the DAG pool increases at the beginning of S phase, whereas the ceramide pool later increases. In the erythroleukemic cells, treated with DMSO and/or D3 vitamin, the N-SMase activity increases during the apoptotic process, whereas PC-PLC activity increases during cell differentiation. It will be interesting to know if DAG and ceramide, produced directly in chromatin, can play a role in cellular functions. 1. Viola Magni M.P., Gahan P.B., Albi E., Iapoce R. and Gentilucci P.F. "Chromatin phospholipids and DNA synthesis in hepatic cells". Bas. Appl. Histochem. 29, 253-259, 1985. I.F. 1.041 2. .Viola Magni M.P., Gahan P.B., Albi E., Iapoce R. and Gentilucci P.F. "Phospholipids in chromatin: incorporation of 32PO24 in different subcellular fraction of hepatocytes" Cell Biochem. and Function 4, 283-288, 1986. I.F. 1.452 3. Viola Magni M.P., Gahan P.B., Albi E., Iapoce R. and Gentilucci P.F. "Synthesis of chromatin phospholipids" Bas. Appl. Histochem. 31, 355-364, 1987. I.F. 1.041 4. Albi E., Viola Magni M.P. and Gahan P.B. "Age-related changes in chromatin phospholipid fraction" The liver, metabolism and ageing, Eurage 13,189-188, 1989. 5. Gahan P.B., Albi E. and Viola Magni M.P. "Changes with age in phospholipid composition of rat liver cell nuclei and nuclear envelopes". Drug metabolism, liver injury and ageing, Eurage, 16, 229-236, 1991. 6. Albi E., Viola Magni M.P., Lazzarini R. and Gahan P.B. "Chromatin phospholipid changes during rat liver development" Cell Biochem. and Function 9, 119-123, 1991. 1.452. I.F. 1.452 7. Albi E. "Presenza e ruolo dei fosfolipidi nella cromatina" Tesi di Dottorato in Patologia Cellulare e Molecolare. 1991. 8. Fraschini A., Albi E., Gahan P.B. and Viola Magni M.P. "Tem cytochemical study of the localization of phospholipids in interphase chromatin in rat hepatocytes" Histochemistry 97, 225-235, 1992. I.F 9. Albi E. , Mersel M., Leray C., Tomassoni M.L. and Viola-Magni M.P. Rat Liver Chromatin Phospholipids" Lipids 29, 715-719- 1994. I.F. 2.164 10. Albi E., Micheli M., Viola-Magni M.P. "Phospholipids and nuclear RNA" Cell Biol. Intern. 20, 6, 1996. I.F. 1.092 11. Albi E., Viola-Magni M.P. "Choline base exchange activity in rat hepatocyte nuclei and nuclear membrane" Cell Biol.Intern 21, 217-221, 1997. I.F. 1.092 12. Albi E., Tomassoni M.L., Viola-Magni M.P. "Effect of lipid composition on rat liver nuclear membrane fluidity" Cell Biochem. and Funct, 15, 181-190, 1997. I.F. 1.452 13. Albi, E. and Viola Magni, M.P. "Chromatin neutral spingomyelinase and its role in hepatic regeneration. Biochim. Biophys. Res. Commun., 236, 29-33, 1997. I.F. 2.836 14. Micheli,M., Albi,E, Leray,C., and Viola Magni,M.P. "Nuclear sphingomyelin protects RNA from RNase action" FEBS Letters 431, 443-447, 1998. I.F. 3.609 15. Tomassoni,M.L., Albi,E., and Viola Magni,M.P. Changes of nuclear membrane fluidity during rat liver regeneration Biochem. Mol. Biol. Intern. 47, 1049-1059, 1999. 16. Albi,E., Peloso,I., and Viola Magni,M.P. Nuclear Membrane Sphingomyelin-Cholesterol Changes in Rat Liver after Hepatectomy Biochem. Biophys. Res. Commun. 262, 692-695, 1999. I.F. 2.836 17. Albi,E., Viola Magni,M.P. Sphingomyelin-Synthase in Rat Liver Nuclear Membrane and Chromatin FEBS Letters 460, 369-372, 1999. I.F. 3.609 18. Albi,E., and Viola Magni,M.P. Phosphatidylcholine-Dependent Phospholipase C in Rat Liver Chromatin Biochem.Biophys.Res.Commun. 265, 640-643, 1999. I.F. 2.836 19. Albi,E., Viola Magni,M.P. The presence and the role of chromatin cholesterol in rat liver regeneration Journal of Hepatology 36, 395-400, 2002. I.F. 5.285 20. Caso,V., Panarelli,P., Albi,E., Viola-Magni,M.P., Parnetti,L., Gallai,V. Phospholipid autoantibodies: time for a new immuno-assay? Clin Exp Hypertens. 24: 511-516, 2002. I.F. 0.816 21. Panarelli,P., Viola-Magni,M.P., and Albi E. Antiphosphatidylinositol antibody in deep venous thrombosis patients Int.J. Immunopath. Pharm. 2003, 16: 61-6. I.F. 3.927 22. Albi,E., Cataldi,S., Rossi,G., and Viola Magni,M.P. A possible role of cholesterol-sphingomyelin/phosphatidylcholine in nuclear matrix during rat liver regeneration. J. Hepatology 2003, 38: 623-8. I.F. 5.283 23. Albi,E., Pieroni,S., Viola Magni,M.P., and Sartori,C Chromatin sphingomyelin changes in cell proliferation and/or apoptosis induced by ciprofibrate. J. Cell Physiol. 2003, 196:354-61. I.F. 5.436 24. Valeria Caso, Lucilla Parnetti, Paolo Panarelli, Maria Pia Viola Magni, MD, Virgilio Gallai, Elisabetta Albi Selection of Thrombogenetic Antiphospholipid Antibodies In Cerebrovascular Disease Patients J.Neurol 2003, 250: 593-597. I.F. 2.778 25. Albi,E., Rossi,G., Maraldi,N.M., Viola Magni,M.P., Cataldi,S., Solimando, L., Zini,N. Involvement of nuclear Phosphatidylinositol-dependent Phospholipases C in cell cycle progression during rat liver J. Cell Physiol. 2003, 197: 181-188. I.F. 5.463 26. Elisabetta Albi, Remo Lazzarini and Mariapia Viola Magni Reverse Sphingomyelin-Synthase in Rat Liver Chromatin FEBS Letters 2003, 549(1-3):152-156. I.F. 3.609 27. Elisabetta Albi, Samuela Cataldi, Mariapia Viola Magni and Claudia Sartori Plasmalogens in rat liver chromatin: new molecules involved in cell proliferation J. Cell Physiol, 2004, 201(3):439-46. I.F. 5.463 28. Elisabetta Albi, Samuela Cataldi, Elisa Bartoccini, Mariapia Viola Magni, Francesca Marini, Francesca Mazzoni, Giuseppe Rainaldi, Monica Evangelisti, Mercedes Garcia-Gil Nuclear sphingomyelin pathway in serum deprivation-induced apoptosis of embryonic hippocampal cells J Cell Physiol, 2005. I.F. 5.463 29. Elisabetta Albi, Caterina AM La Porta , Samuela Cataldi, Mariapia Viola Magni Nuclear sphingomyelin-synthase and protein kinase C delta in melanoma cells Arch. Biochem Biophys, 2005, 438:156-61. I.F. 2.338

scientific

Department of Clinical and Experimental Medicine, Physiopathology, University School of Medicine

University of Perugia

++39-075-5729085

06100

Perugia

Policlinico Monteluce, via Brunamonti

Italy

intranuclear lipid, DNA-lipid, RNA-lipid

We have demonstrated that a phospholipid cellular fraction is associated with chromatin. The presence, described by histochemical and biochemical techniques, is not due to membrane contaminations. The chromatin phospholipid fraction (CPF) can be considered a chromatin minor component. The CPF differ from that of microsomes and nuclear membranes as regard composition and turnover and changes in relation to hepatocyte maturation and proliferation. The main modifications, evident in several cellular functions, regard principally phosphatidylcholine (PC) and sphingomyelin (SM) that are metabolised directly in the nuclei in a very short time with a fine modulation of the enzimatic activities, regulated by PLs. The presence of a complex of base exchange enzymes for PC synthesis, a neutral sphingomyelinase (N-SMase), a sphingomyelin-synthase (SM-synthase), a phosphatidylcholine-dependent phospholipase C (PC-PLC) and a sphingomyelin-synthase-reverse (SM-synthase-reverse), which synthesises PC from SM have been demonstrated. These enzymes differ for pH and Km optima from those present in nuclear membranes, suggesting the possible existence of different isoforms. These results strongly support the presence of a metabolic machinery in the chromatin which probably is regulated in relation to cellular function. The PC-PLC and SM-synthase enrich the intranuclear pool of diacylglicerol (DAG), whereas the N-SMase and SM-synthase-reverse enrich the intranuclear pool of ceramide. DAG and ceramide are know as second messengers which play an important role in different physiological conditions, acting as a stimulating (DAG) and inhibitory factor (ceramide) of protein kinase C (PKC) The hypothesis has been made that the lipid messenger can favour the nuclear molecular events which precede DNA synthesis in liver regeneration. The results show that the DAG pool increases at the beginning of S phase, whereas the ceramide pool later increases. In the erythroleukemic cells, treated with DMSO and/or D3 vitamin, the N-SMase activity increases during the apoptotic process, whereas PC-PLC activity increases during cell differentiation. It will be interesting to know if DAG and ceramide, produced directly in chromatin, can play a role in cellular functions. 1. Viola Magni M.P., Gahan P.B., Albi E., Iapoce R. and Gentilucci P.F. "Chromatin phospholipids and DNA synthesis in hepatic cells". Bas. Appl. Histochem. 29, 253-259, 1985. I.F. 1.041 2. .Viola Magni M.P., Gahan P.B., Albi E., Iapoce R. and Gentilucci P.F. "Phospholipids in chromatin: incorporation of 32PO24 in different subcellular fraction of hepatocytes" Cell Biochem. and Function 4, 283-288, 1986. I.F. 1.452 3. Viola Magni M.P., Gahan P.B., Albi E., Iapoce R. and Gentilucci P.F. "Synthesis of chromatin phospholipids" Bas. Appl. Histochem. 31, 355-364, 1987. I.F. 1.041 4. Albi E., Viola Magni M.P. and Gahan P.B. "Age-related changes in chromatin phospholipid fraction" The liver, metabolism and ageing, Eurage 13,189-188, 1989. 5. Gahan P.B., Albi E. and Viola Magni M.P. "Changes with age in phospholipid composition of rat liver cell nuclei and nuclear envelopes". Drug metabolism, liver injury and ageing, Eurage, 16, 229-236, 1991. 6. Albi E., Viola Magni M.P., Lazzarini R. and Gahan P.B. "Chromatin phospholipid changes during rat liver development" Cell Biochem. and Function 9, 119-123, 1991. 1.452. I.F. 1.452 7. Albi E. "Presenza e ruolo dei fosfolipidi nella cromatina" Tesi di Dottorato in Patologia Cellulare e Molecolare. 1991. 8. Fraschini A., Albi E., Gahan P.B. and Viola Magni M.P. "Tem cytochemical study of the localization of phospholipids in interphase chromatin in rat hepatocytes" Histochemistry 97, 225-235, 1992. I.F 9. Albi E. , Mersel M., Leray C., Tomassoni M.L. and Viola-Magni M.P. Rat Liver Chromatin Phospholipids" Lipids 29, 715-719- 1994. I.F. 2.164 10. Albi E., Micheli M., Viola-Magni M.P. "Phospholipids and nuclear RNA" Cell Biol. Intern. 20, 6, 1996. I.F. 1.092 11. Albi E., Viola-Magni M.P. "Choline base exchange activity in rat hepatocyte nuclei and nuclear membrane" Cell Biol.Intern 21, 217-221, 1997. I.F. 1.092 12. Albi E., Tomassoni M.L., Viola-Magni M.P. "Effect of lipid composition on rat liver nuclear membrane fluidity" Cell Biochem. and Funct, 15, 181-190, 1997. I.F. 1.452 13. Albi, E. and Viola Magni, M.P. "Chromatin neutral spingomyelinase and its role in hepatic regeneration. Biochim. Biophys. Res. Commun., 236, 29-33, 1997. I.F. 2.836 14. Micheli,M., Albi,E, Leray,C., and Viola Magni,M.P. "Nuclear sphingomyelin protects RNA from RNase action" FEBS Letters 431, 443-447, 1998. I.F. 3.609 15. Tomassoni,M.L., Albi,E., and Viola Magni,M.P. Changes of nuclear membrane fluidity during rat liver regeneration Biochem. Mol. Biol. Intern. 47, 1049-1059, 1999. 16. Albi,E., Peloso,I., and Viola Magni,M.P. Nuclear Membrane Sphingomyelin-Cholesterol Changes in Rat Liver after Hepatectomy Biochem. Biophys. Res. Commun. 262, 692-695, 1999. I.F. 2.836 17. Albi,E., Viola Magni,M.P. Sphingomyelin-Synthase in Rat Liver Nuclear Membrane and Chromatin FEBS Letters 460, 369-372, 1999. I.F. 3.609 18. Albi,E., and Viola Magni,M.P. Phosphatidylcholine-Dependent Phospholipase C in Rat Liver Chromatin Biochem.Biophys.Res.Commun. 265, 640-643, 1999. I.F. 2.836 19. Albi,E., Viola Magni,M.P. The presence and the role of chromatin cholesterol in rat liver regeneration Journal of Hepatology 36, 395-400, 2002. I.F. 5.285 20. Caso,V., Panarelli,P., Albi,E., Viola-Magni,M.P., Parnetti,L., Gallai,V. Phospholipid autoantibodies: time for a new immuno-assay? Clin Exp Hypertens. 24: 511-516, 2002. I.F. 0.816 21. Panarelli,P., Viola-Magni,M.P., and Albi E. Antiphosphatidylinositol antibody in deep venous thrombosis patients Int.J. Immunopath. Pharm. 2003, 16: 61-6. I.F. 3.927 22. Albi,E., Cataldi,S., Rossi,G., and Viola Magni,M.P. A possible role of cholesterol-sphingomyelin/phosphatidylcholine in nuclear matrix during rat liver regeneration. J. Hepatology 2003, 38: 623-8. I.F. 5.283 23. Albi,E., Pieroni,S., Viola Magni,M.P., and Sartori,C Chromatin sphingomyelin changes in cell proliferation and/or apoptosis induced by ciprofibrate. J. Cell Physiol. 2003, 196:354-61. I.F. 5.436 24. Valeria Caso, Lucilla Parnetti, Paolo Panarelli, Maria Pia Viola Magni, MD, Virgilio Gallai, Elisabetta Albi Selection of Thrombogenetic Antiphospholipid Antibodies In Cerebrovascular Disease Patients J.Neurol 2003, 250: 593-597. I.F. 2.778 25. Albi,E., Rossi,G., Maraldi,N.M., Viola Magni,M.P., Cataldi,S., Solimando, L., Zini,N. Involvement of nuclear Phosphatidylinositol-dependent Phospholipases C in cell cycle progression during rat liver J. Cell Physiol. 2003, 197: 181-188. I.F. 5.463 26. Elisabetta Albi, Remo Lazzarini and Mariapia Viola Magni Reverse Sphingomyelin-Synthase in Rat Liver Chromatin FEBS Letters 2003, 549(1-3):152-156. I.F. 3.609 27. Elisabetta Albi, Samuela Cataldi, Mariapia Viola Magni and Claudia Sartori Plasmalogens in rat liver chromatin: new molecules involved in cell proliferation J. Cell Physiol, 2004, 201(3):439-46. I.F. 5.463 28. Elisabetta Albi, Samuela Cataldi, Elisa Bartoccini, Mariapia Viola Magni, Francesca Marini, Francesca Mazzoni, Giuseppe Rainaldi, Monica Evangelisti, Mercedes Garcia-Gil Nuclear sphingomyelin pathway in serum deprivation-induced apoptosis of embryonic hippocampal cells J Cell Physiol, 2005. I.F. 5.463 29. Elisabetta Albi, Caterina AM La Porta , Samuela Cataldi, Mariapia Viola Magni Nuclear sphingomyelin-synthase and protein kinase C delta in melanoma cells Arch. Biochem Biophys, 2005, 438:156-61. I.F. 2.338

scientific

Department of Internal Medicine

University of Perugia

0039 75 5857423

06122

Perugia

Via del Giochetto

Italy

pollen phospholipids, brain mitochondria, anionic phospholipids, cardiolipin

I)Plant pollens are an important source of environmental antigens that stimulate allergic responses. In addition to acting as vehicled for foreign protein antigens, they contain different molecular species of lipids, which are necessary in the reproduction of higher plants. The CD1 family of nonpolymorphic major histocompatibility complex-related molecules is highly conserved in mammals, and has been shown to present microbial and self lipids to T cells. We provided evidence that pollen lipids may be recognized as antigens by human T cell through a CD1-dependent pathway. Amonng phospholipids extracted from cypress grains, phosphatidylcholine (PC)and phosphatidylethanolamine (PE)were able to stimulate the proliferation of T cells from cypresse-sensitive subjects. Particularly, 18:2/18:2 PC, a predominat component of cypress PC, could be one of the specific targets in pollen grain capture and recognition by CD1. Also pollen-derived unsaturated PE are antigens recognized by a variety of T clones derived from allergic subjects. The nature of the acyl chains in the lipid antigens contributes enormously to immunogenicity.(Agea et al. Journal Exp. Med. (2005) 202:295-308. The nature of lipid-protein interaction between phospholipids and CD1 is a target for future investigation. The antigenic properties of glycolipids extracted and purified from pollens will be also evaluated. II) We are also involved in projects aimed at studying the role of anionic phospholipids, particularly cardiolipin, in the functionality of mitochondria in the nervous tissue. A major finding of our previous studies was the effect exerted by exogenous phospholipids on the release of cytochrome c from the inner mitochondrial membrane and on the transmembrane potential of brain mitochondria. Mitochondria were enriched with exogenous phosoholipids through a fusion process in the outer mitochondrial membrane. The experimental system utilized a fluorescent probe inserted in the outer mitochondrial membrane to follow the kinetics of incorporation of lipids in mitochondria. By using a selective probe for cardiolipin, it has been demonstrated thet the fused lipid can reach the inner mitochondrial membrane and influence the membrane potential. We found that exogenous cardiolipin reinforced the binding of cytochrome c with the inner mitochondria, thus hindering its release following different stimuli. The interaction of cytochrome c with the components of the inner mitochondrial membrane is object of investigation. The mode of membrane association of cytochrome c is studied by using a model system of cytochrome c reconstituted in different cardiolipins. (Piccotti et al. JBC 2002, 277:12075-12081, Piccotti et al. J. Membr. Biol. 2004, 198:43-53)

scientific

Department of Internal Medicine, Section of Biochemistry

University of Perugia

++39-075-585-7420

I-06122

Perugia

Via del Giochetto

Italy

phospholipase A2, lipid mediators, PAF

This group is interested in the identification and localization of various phospholipases A2 in intracellular compartments. Particular attention is devoted to secretory PLA2s with the aim of uderstanding their functions in normal and pathological conditions. We have already demonstrated the presence of a group IIA sPLA2 from cerebral cortex mitochondria and its release under energy-deficient conditions and the location of group V sPLA2 in the nuclei of cultured PC-12 and U251 astrocytome cells of group V sPLA2 (Macchioni et al., J Biol Chem. (2004) Vol. 279, 36 (3), 37860-37869). Furthermore we have set up a procedure for a continuous monitoring PLA2 activities in cultured cells in vivo or subcellular organelles. Since many cell types release low molecular weight sPLA2, we are also studying the routing for their secretion of their translocation to intracellular compartments. This group is also interested on the formation of lipid mediators and particularly of PAF. We have identified the presence of the enzymes for its synthesis in the nervous tissue.

scientific

Department of Internal Medicine, Section of Biochemistry

University of Perugia

++39-075-585-7420

I-06122

Perugia

Via del Giochetto

Italy

phospholipase A2, lipid mediators, PAF

This group is interested in the identification and localization of various phospholipases A2 in intracellular compartments. Particular attention is devoted to secretory PLA2s with the aim of uderstanding their functions in normal and pathological conditions. We have already demonstrated the presence of a group IIA sPLA2 from cerebral cortex mitochondria and its release under energy-deficient conditions and the location of group V sPLA2 in the nuclei of cultured PC-12 and U251 astrocytome cells of group V sPLA2 (Macchioni et al., J Biol Chem. (2004) Vol. 279, 36 (3), 37860-37869). Furthermore we have set up a procedure for a continuous monitoring PLA2 activities in cultured cells in vivo or subcellular organelles. Since many cell types release low molecular weight sPLA2, we are also studying the routing for their secretion of their translocation to intracellular compartments. This group is also interested on the formation of lipid mediators and particularly of PAF. We have identified the presence of the enzymes for its synthesis in the nervous tissue.

scientific

Funato Group

Hiroshima University

++81-82-424-7925

739-8528

Higashi-Hiroshima

1-4-4, Kagamiyama

Japan

ceramide, GPI, transport

lipid trafficking

scientific

Department of Biochemistry and Molecular Biology

Osaka University Graduate School of Medicine

++81-6-6879-3283

662-0018

Suita

2-2 Yamadaoka

Japan

HPLC/MS, sphingolipids, phospholipids

I has developed an HPLC/ESI/MS-based method capable of analyzing a wider range of lipid classes from neutral lipids to phospholipids on a single chromatographic run with three solvent gradients and post-column mixing of solvent that helps ESI of lipids eluted earlier with non-polar solvents. We used this method to study on lipid anomaly of a variety of gene-targeted mice tissues and subcellular organellae [e.g., Takagi, S., Tojo, H., Tomita, S., Sano, S., Itami, S., Hara, M., Inoue, S., Horie, K., Kondoh, G., Hosokawa, K., Gonzalez, F.J. and Takeda, J. Alteration of the 4-sphingenine scaffolds of ceramides in keratinocyte-specific Arnt deficient mice affects skin barrier function. J. Clin. Invest. 112, 1372-1382 (2003)]. We are now extending the method amenable to analyzing more polar lipids that cantain important bioactive lipids. We eventually would like to develop a very comprehensive lipid analyzing system.

scientific

Department of Metabolome, Graduate School of Medicine

The University ofTokyo

81-3-5841-3651

113-0033

Tokyo

7-3-1, Hongo, Bunkyo-ku

Japan

lipid, database

create lipid database "LipidBank"

scientific

Laboratory Genetic Metabolic Diseases

Academic Medical Center

+31-20-5669111

NL-1100 DD

Amsterdam

P.O. Box 22660

Netherlands

tandem-Mass Spectrometry, glycerophospholipids, cardiolipin, ceramides

HPLC-tandem mass spectrometric analysis of lipid compositions at different levels of complexity, such as cell compartments/organelles, cells, tissues in order to clarify/study biochemical processes in relation to disease based disorders. Research is biomedical orientated in a university hospital setting and findings are where possible translated into diagnostic tests.

scientific

DSM

analysis/spectrometry

0031 152792278

2600 MA

Delft

p.o. box 1

Netherlands

ceramides, sphingolipids

ceramides, sphingolipids

industrial

Lipid laboratory

Dept Internal Medicine, UMC Nijmegen

-

6500 HB

Nijmegen

Geert Grooteplein zuid 8

Netherlands

GC-MS, steroids, lipoproteins

lipidperoxydation and antioxidants, lipid transfer, steroids, nutrients

scientific

Physics of Life Processes

Leiden Institute of Physics, Leiden University

+31 71 527 5982

2333 CA

Leiden

Niels Bohrweg 2

Netherlands

cell signaling, model systems, lipid rafts, single-molecule biophysics, fluorescence microscopy

cell signaling, model systems, lipid rafts, single-molecule biophysics, fluorescence microscopy

scientific

NIOZ

NIOZ

31 222 369582

1790 AB

Den Burg

P.O. Box 59

Netherlands

organic geochemistry, biomarker

As part of the ICOMM (http://icomm.mbl.edu/)work we are building up a lipid database. If possible, we want to combine our data with yours. Firstly, we are interested in a procedure to find data from the database. What programs are developed, how do they work. If there is no such program exists, one has to be created. We hope we can work with you to get things working.

scientific

Philips Research, Molecular Diagnostics

Philips

+31-40-2743794

5656 AE

Eindhoven

High Tech Campus 4

Netherlands

cholsterol, membrane microdomains, sphingolipids, oxidized lipids, lipoproteins

Bioactive lipids relevant to diseases

industrial

Bijvoet Center

Utrecht University

++31-30-2533498

NL-584 CH

Utrecht

Padualaan 8

Netherlands

membrane membrane-protein rafts

Lipids is membranes and as signalling entities

scientific

Institute of Biomembranes

Utrecht University

+31-30-2536616

3584 CH

Utrecht

Padualaan 8

Netherlands

lipid cell biology - lipid mass spectrometry - model membrane biochemistry

The Institute of Biomembranes is an interdisciplinary research institute and graduate school at Utrecht University, accommodating 18 research groups from the faculties of Science, Medicine, and Veterinary Medicine. The famous lipid biochemist Laurens van Deenen was one of the founding members of the institute in 1991, but unfortunately deceased in 1994. To remember him, since 2004 the Institute annually awards the "van Deenen Medal" to a leading active scientist in biomembrane research (http://ib.bio.uu.nl/). The present director chairs a specific support action of the European Commission entitled "The European Lipidomics Initiative" (www.lipidomics.net). A number of groups in the institute work on questions related to the structure and function of lipids in cells, blood (lipoproteins) and model membranes. Notably, a dedicated effort in lipid mass spectrometry is ongoing in the Dept. of Biochemistry and Cell Biology, Faculty of Veterinary Medicine (http://www.vet.uu.nl/bc).

scientific

Nutrition, metabolism and genomics group

Wageningen University

++31-317-485787

6703 HD

Wageningen

Bomenweg 2

Netherlands

transcriptomics, PPARs, fatty acids

Our group is interested in regulation of gene expression by fatty acids. We use transcriptomics (in house affymetrix platform) in combination with knock-out mice models to investigate the overall impact as well as the mechanism of fatty acid-dependent gene regulation in numerous organs (small intestine, liver, heart). Our expertise is: 1) design, analysis and interpretation of transcriptomics experiments, and 2) pathway mapping of transcriptomics data with special emphasis on lipid metabolism

scientific

Preclinical sciences

GE Healthcare

+47 2318 5666

0401

Oslo

Nycoveien 2

Norway

Phospholipids, blood, drug development

Phospholipid analysis related to contrast agents for medical imaging. Analyses of blood samples in order to describe pharmacokinetics/toxicokinetics.

industrial

Inst. for Cancer Research/Sandvigs group:Intracellular transport

The Norwegian Radium Hospital

47 22934294

0310

Oslo

Montebello

Norway

glycosphingolipids, toxins, cholesterol, rafts

Studies of the role of different lipids on intracellular transport of protein toxins. For recent publications, please see the home pages:http://radium.no/sandvig/ We have been studying the role of cholesterol in transport, and we have been investigating cells with mutations in (glyco)sphingolipid synthesis. Studies on the roles of glycospingolipids are in progress.

scientific

Laboratory of Transcriptional Regulation

Centre for Medical Biology PAS

++48-42-2723639

93-232

Lodz

Lodowa 106

Poland

ABC transporters, gene expression, transcription factors

Lipid hormones and second messengers - their direct and indirect effects on gene expression. Lipid-binding transcription factors. Lipid transporters from the ABC superfamily - mechanism of action, regulation of expression.

scientific

Department of Animal Physiology and Biophysics

Faculty of Biology, University of Bucharest

00-40-21-318 15 69

050095

Bucharest

Splaiul Independentei, 91-95

Romania

Plane lipid bilayers, Liposomes, Pharmacology

Our group is interested to test antidepressants (natural and synthetic) and neuroleptics on plane lipid bilayers by electrical recordings and liposomes by fluorescence polarisation.

scientific

Institute of Biophysics, Faculty of Medicine

University of Ljubljana

-386 1 5437600

1000

Ljubljana

Lipiceva 2

Slovenia

vesicle shapes, membrane trafficking, amphitropic proteins

Mechanical properties of lipid membranes. Theoretical and experimental studies of phospholipid vesicle shape behavior. Shape induced mechanisms for the lateral segregation of membrane components. Role of vesicle shapes in intracellular membrane trafficking. Mechanism of action of amphitropic proteins studied by vesicle shape transformations induced by their binding to vesicle membranes.

scientific

INSTITUTO DE PARASITOLOGIA Y BIOMEDICINA LOPEZ-NEYRA, Working Group: BIOCHEMISTRY AND MOLECULAR PHARMACOLOGY. Dr. FRANCISCO GAMARRO

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS

0034958181667

18100

ARMILLA- GRANADA

Parque Tecnológico de Ciencias de la Salud. Avda. del Conocimiento s

Spain

Parasites, lipid translocation, Aminophospholipid translocases, ABC transporters.

Lipid translocation in arasites of health interest: their implications in the biology of parasites, and their potential use as drug targets. Aminophospholipid translocases and ABC transporters in parasites: its role in drug resistance and infectivity of parasites.

scientific

IQAC/Research Unit on BioActive Molecules

CSIC

34-93-4006115

08034

Barcelona

Jordi Girona 18

Spain

combinatorial chemistry, nuclear magnetic ressonance, drug discovery, environmental contaminants

Drug discovery and pharmacological tools: Effects of combinatorial libraries of synthetic chemicals on cell sphingolipid profiles. Toxicity of environmental contaminants: Deciphering whether the toxicity of selected environmental contaminants occurs by altering the sphingolipid composition of affected cells. Sphingolipid maps as sensors of environmental contamination. Analytical methodology: (1) Development of 15N and 31P NMR procedures to construct sphingolipid and phospholipid maps. (2) Application of biocompatible chemical reactions to the construction of pseudolipid maps and validation of this approach to research in cell biology.

scientific

Unidad de Biofísica

CSIC and Universidad del País Vasco

++34-94-601-2625

48940

Leioa

Sarriena s

Spain

membrane lipids, lipid biophysics, membrane domains, lipid-protein interaction

We are interested in the biophysics of membrane lipids, and lipid-protein interactions, with an emphasis on sphingolipids, lipid signalling, membrane domains, and, more recently, lipoproteins. Our techniques include calorimetry (DSC, ITC), spectroscopy (IR, fluorescence, UV-vis, stopped-flow, CD), confocal microscopy, Langmuir balance, and supported membranes.

scientific

National Center of Biotechnology

Spanish Research Council

++34-91-5854840

28049

Madrid

Darwin, 3. Campus Cantoblanco University

Spain

signaling, chemotaxis, leukocyte, HIV

The previous work of my group has been focused in understand how lipid rafts organize cell signaling during leukocyte chemotaxis as well as in the pivotal function of these microdomains at the early steps of the human immunodeficiency virus infection.

scientific

Institute of Molecular Biology and Genetics

Spanish Research Council & University of Valladolid School of Medicine

+34-983-423-062

E-47003

Valladolid

Calle Sanz y Fores s

Spain

Phospholipase A2, Arachidonic Acid, Prostaglandins, Lysophosphatidylcholine

Eicosanoids, Lysophospholipids, Glycerophospholipids

scientific

BIOMIL (BIOphysics of Membranes and Lipid/Protein Interfaces)

Universidad Complutense

++34-91-3944994

28040

Madrid

Fac. Biologia, Dept. Bioquimica

Spain

pulmonary surfactant, monolayers, membrane domains, surface activity

Membrane lateral structure and its role in the establishment of lipid-protein and protein-protein interactions. Lipids and lipid/protein complexes as surface-active agents, with particular emphasis in the structure and molecular mechanisms of membranes and surface layers of natural pulmonary surfactant and clinical preparations used in the therapeutical treatment of respiratory diseases.

scientific

Research Group in Biomembranes, Department of Biochemistry and Molecular Biology

University of Murcia

++34-968364766

E-30100

Murcia

Facultad de Veterinaria, Campus de Espinardo

Spain

lipid-protein interaction, PKCs, diacylglycerol, liposomes

Protein-lipid interactions: interactions with model membranes of signalling proteins, such as PKCs and Bcl-2 family of proteins associated to the regulation of cell apoptosis. Lipid regulation of the activity of PKCs. The action of a variety of bioactive lipìds on membrane translocation and activation of the Protein Kinase C family of proteins associated to cell signalling. The action of lipids such as phosphatidylserine, phosphoinositides, free fatty acids, diacylglycerols and ceramides is invetigated. Techniques used: NMR (CPMAS, HRMAS, deuterium NMR, 31P-NMR), DSC, ITC, Biacore, Fluorescence spectroscopy, confocal microscopy, X-ray diffraction.

scientific

Dept. Physiology, Medical School

University of the Basque Country

++34946012846

48940

Bilbao

Sarriena

Spain

Cholesterol, Liver, VLDL secretion, functional genomics

Disorders of lipid and lipoprotein metabolism involved in hepatic and cardiovascular diseases. Molecular mechanisms underlying nonalcoholic fatty liver disease. Functional genomics in nonalcoholic fatty liver disease.

scientific

The Wenner-Gren Institute

Stockholm University

++46-8-164127

SE10691

Stockholm

Arrhenius lab. F3

Sweden

fatty acid metabolism, elongase, gene expression

Our topic is the regulation of very long chain fatty acid syntersis in mammals and their significance in lipid metabolism.

scientific

Department of Crop Science

Swedish University of Agricultural Sciences

please fill in

23053

Alnarp

Box 44

Sweden

plant oil biosynthesis

please fill in

scientific

Department of Biophysical Chemistry

Umeå University

+46907865228

SE-90187

Umeå

Linaeus väg 10

Sweden

domains, lateral diffusion, Lipid/protein

Lipid/protein interaction, regulation of lipid composition in cell membranes, Formation of domains in lipid membranes, Lipid lateral diffusion, Molecular ordering in membranes,.

scientific

LC-MS Support

Applera Europe B.V.

+41 41 799 7742

6343

Rotkreuz

Grundstrasse 10

Switzerland

phospholipid, eiscosanoids, PAF, biomarker

Understanding of succession at developing and self stabilising cell populations and response/adaptation in the membrane lipid composition to stress conditions. Reveal cell signaling processes and interactions with the environment depending on lipid molecules. Lipid synthesis and degradation depend on enzyme activities and specific genes. Supressing specific lipid synthesis process or facilitating adaptation processes may identify genes involved and clarify function of so far unknown gene sequences. Assessment of regulatory lipids (eicosanoids, PAFs) as presymptomatic harbingers of pulmonary pathobiology. Development of rapid and easy-to-use methods for assessemnt and analysis of phospholipid profiles based on mass spectrometry. Rapid and sensitive quantification of signaling lipid molecules. Since 2007 LC-MS Support for Switzerland and South of germany Area, providing expertise in Lipidanalysis by LC-MS

industrial

Life Sciences Mass Spectrometry

School of Pharmaceutical Sciences - University of Geneva

+41-22-3796344

1211

Geneva

Bd Yvoy 20

Switzerland

Mass spectrometry, Pharmaceuticals, Proteins, C.elegans

please fill in

scientific

University of Basel, Experimental Immunology

University Hospital, Department of Research

+41 61 2652365

4031

Basel

Hebelstrasse, 20

Switzerland

immune recognition of lipids

Identification of llipids with immunogenic activity and involved in activating lipid-specific T cells in diseases

scientific

Division of Biochemistry

University of Fribourg

0041 26 300 8630

CH-1700

Fribourg

5, chemin du musée

Switzerland

Ceramide, GPI, cell wall, heat shock, cholesterol, ergosterol, steryl esters, lipases, lipid bodies

Biosynthesis, Remodeling and transport and cell wall integration of GPI anchored proteins of Saccharomaces cerevisiae. Sphingolipid biosynthesis of S. cerevisiae. Sterol transport, neutral lipid storage and degradation. Lipid rafts transport and sortining of integral membrane proteins.

scientific

Institute of Cellular and Organismic Biology, Section of Stem Cells

Academia Sinica

++886-2-27899531

11529

Taipei

Nankang

Taiwan

farnesyl pyrophosphate, geranylgeranyl pyrophosphate, E-Ras

Prenyl pyrophosphates as differentiation signals for stem cells

scientific

Institute of Bioinformatics and Structural Biology

National Tsing Hua University

886-3-5742752

30043

Hinchu

Kung-Fu Rd

Taiwan

glycosphingolipid, cardiotoxin, phospholipase A2

Our main interest is to understand the role of various lipids and/or lipid domain responsible for the action of cobra venom components such as cobra cardiotoxins or phospholipase A2.

scientific

Dept. of Medical Biochemistry & Immunology, School of Medicine

Cardiff University

0044 29 2074 8447

CF14 4XN

Cardiff

Heath Park

United Kingdom

please fill in

please fill in

scientific

Chemical Engineering Life Science Interface

Chemical and Process Engineering University of Sheffield

+44-07809717355

S3 7RD

Sheffield

40 LeavyGreave Road

United Kingdom

Sulfolipids, sulfoglycolipids, cyanobacteria, bioactive compounds

My area of interest is to mining lipid profile of cyanobacteria for identifying potential drug candidates.

scientific

School of Life Sciences

Heriot-Watt University

++44 131 451 3186

EH14 4AS

Edinburgh

Roccarton

United Kingdom

Diet-gene interaction, lipogenesis,fungal lipid metabolism

We are interested in the influence of diet on the regulation of gene expression in hepatic tissue culture, in particular emphasis on lipogenic genes. A second area of interest focusses on fungal lipid metabolism, particularly in the oleaginous Yarrowia lipolytica.

scientific

The Chemical Biology Centre in the Department of Chemistry

Imperial College London in association with the Institute of Cancer Research and the London Research Institute of CRUK

+44 (0)20 7594 5787

SW7 2AZ

London

Exhibition Road

United Kingdom

Lipid biophysical characterisation

Biophysical properties of lipids Phase behaviour studied by SAXRD, NMR, SSNMR, polarising microscopy, DSC, high pressure SAXRD Mesophase structure and energetics measured by SAXRD osmometry, SSNMR, ITC, DSC, direct vesicle manipulation and modelling using continuum elastic theories, mesoscopic modelling and full atomistic modelling (QMMD) Dynamics of phase transformations (the lipids role in cell division, fusion etc.) using synchrotron X-ray scattering on rapidly perturbed systems Studies of the short and long range coupling of membrane charge and curvature elasticity Lipid-protein and lipid-drug interactions Studies of the effect of membrane elasticity on enzyme activity, extrinsic membrane protein binding, membrane protein refolding dynamics, drug binding Studies of the coupling of membrane chemistry and biochemistry to lipid elastic properties Studies of lipid liquid ordered phases and protein associations within these domains Studies of membrane protein assembly, distribution and dynamics Development of novel single cell membrane proteomics and lipidomics A recently funded project to handle single cells inoptical traps within microfluidic flows, strip off membrane portions, separate components and analyse using a novel 2D-IR spectroscopy

scientific

Nature Reviews Molecular Cell Biology

NPG

+44(0)2078433641

N1 9XW

London

4 Crinan St

United Kingdom

lipids

lipids

scientific

Glycobiology Institute

Oxford University

+44 1865 275725

OX1 3QU

Oxford

South Parks Rd

United Kingdom

Oligosaccharide analysis, lysosomal storage

Protein and lipid glycosylation and diseases, including the lysosomal storage disorders (Gaucher disease for example). Glycolipid (sphingolipid) analysis by HPLC and mass spectrometry. Chemistry and biology of imino sugars that modulate glycolipid biosynthesis

scientific

Shell Global Solutions (UK)

Shell Global Solutions (UK)

+44-151-373-5730

CH13SH

Chester

P.O. Box 1

United Kingdom

extraction analysis

Interest in extraction and analysis techniques for lipids

industrial

Refsum disease group

St Thomas Hospital

++442071881256

SE1 7EH

London

Lambeth Palace Road

United Kingdom

peroxisome, alpha-oxidation, omega-oxidation, isoprenoid, phytanic

The group has been interested in the transport and biochemical pathways of phytanic acid metabolism and thus alpha- and omega-oxidation pathways of alpha-methyl isoprenoid fatty acids. This pathway has recently been defined with the cloning of key enzymes in alpha-oxidation including phytanoyl-CoA 2-hydroxylase (PAHX), 2-hydroxyphytanoyl-CoA lyase, alpha-methylacyl-CoA racemase together with confirmation of their localisation in peroxisomes. PAHX, an iron(II) and 2-oxoglutarate dependent oxygenase is located on chromosome 10p13. Mutant forms of PAHX have been shown to be responsible for some, but not all, cases of Refsums Disease. Certain cases have been shown to be atypical mild variants of rhizomelic chondrodysplasia punctata type 1a. Other atypical cases with low plasma phytanic acid may be caused by a-methylacyl-CoA racemase deficiency. A sterol-carrier protein 2 (SCP-2) knockout mouse model shares a similar clinical phenotype to Refsums Disease, but no mutations in SCP-2 have been described to date in man. SCP-2 acts as a solubilistaion factor/intracellular carrier for these hydrophobic fatty acids. Work on the omega-oxidation pathway for these fatty acids has clarified the role of cytochrome 4A1 enzymes in the intitial hydroxylation to dicarboxylic acids and the subsequent peroxisomal beta-oxidation pathway. It has also led to interest in this pathway as a therapeutic option for peroxiosomal diseases. The pathway for synthesis of phytanic acid from phytol in man and possibly other mammals has also been clarified with the identification of the enzymes in the pathway and identification of FALDH-10 - the enzyme deficient in Sjogren-Larsson syndroem as one of the key enzymes in this pathway. The group is also interested in the role of PhyH outside the preroxisome where it may act as protein regulator and the possible importance of phytanic acid as a PPAR-alpha ligand.

scientific

Mass Spectrometry Facility

The School of Pharmacy

++44-20-77535876

WC1N 1AX

London

29/39 Brunswick Square

United Kingdom

sterols, steroids, lipid rafts

The research in the mass spectrometry group at the School of Pharmacy is focused on lipids and their interactions with proteins. Major efforts are being made in the development of new methodology for the mass spectrometric analysis of steroids and sterols. In parrallel with lipidomic studies, we are investigating the protein content of lipid rafts and studying protein-lipid interactions.

scientific

European application laboratory

Thermo Electron

+44 1442233555

HP2 7GE

Hemel Hempstead

1 Boundary Park

United Kingdom

mass spectrometry, phospholipids, sphingolipids

methods in mass spectrometry for high throughput lipid analysis

industrial

WELLCOME TRUST BIOCENTRE, SCHOOL OF LIFE SCIENCES, UNIV OF DUNDEE

UNIV OF DUNDEE

++44-(0)1382-388688

DD1 5EH

DUNDEE

HAWKHILL

United Kingdom

PROTOZA LIPID BIOSYNTHESIS

MY GROUP WORKS ON PHOSPHO- AND GLYCO- LIPID BIOSYNTHESIS IN PROTOZOAN PARASITES SUCH AS T.BRUCEI, THE CAUSATIVE AGENT OF AFRICAN SLEEPING SICKNESS. WE USE TOOLS SUCH AS FOWARD AND REVESRE GENETICS TO VALIDATE GENES AS DRUG TARGETS PRIOR TO EXPLORING SUBSTRATE AND INHIBITOR SELECTIVITY. WE HAVE START TO SET UP A PARASITE LIPIIDOME DATABASE FOR THE COMMUNITY TO USE AND SUBMIT PROFILES TO.

scientific

Cardiovascular Genetics,

University College London

+44 207 679 6968

WC1E 6JF

London

5 University St

United Kingdom

genes, apolipoproteins, triglyceridie metabolism

Cardiovascular Genetics

scientific

Aberdeen lipidomix group

University of Aberdeen

44 (0)1224 553020

AB25 2ZD

Aberdeen

Polwarth building, Foresterhill

United Kingdom

inflammation-atherosclerosis-endothelial fonction-diet

function of biologically active lipids in cardiovascular disorders. Effect of dietary components on the expression and secretion of biologically active lipid derivatives in relation to inflammatory disorders.

scientific

Department of Pharmacy & Pharmacology

University of Bath

++-44-1225-3867686

BA2 7AY

Bath

Claverton Down

United Kingdom

Enzyme, phytanic acid, isoprenoids, long-chain fatty alcohols

Enzaymes involved in lipid metabolism, branched-chain lipids and their role in diseases, long-chain fatty alcohols,

scientific

Institute for Cancer Studies

University of Birmingham

44-(0)121-414-3293

B15 2TT

Birmingham

Vincent Drive

United Kingdom

phosphoinositide, mass spectrometry, phospholipase, cancer

1. Analysis of lipid signalling in particular phospholipases D and C and PI-3-kinase. 2. Use of MS methods to analyse lipids in mammalian, dictyostelium, yeast and drosophila cells in order to determine functions of signalling pathways. 3. Analysis of lipids in tumour cells isolated by techniques such as laser capture microdissection. 4. Analysis of changes in lipids in other diseases for example vasculitis. 5. Development of MS methods to quantify all phosphoinositides.

scientific

School of Pharmacy

University of Bradford

++44-1274-224717

BD7 1DP

Bradford

Richmond Road

United Kingdom

eicosanoids, brain, skin, mass spectrometry

eicosanoids and other lipid mediators molecular mechanism of action of omega-3 fatty acids with emphasis on eicosapentaenoic acid eicosanoid-mediated pathways and signalling systems mass spectrometry high field NMR computational lipidomics cardiovascular disease neurodegenerative diseases brain function cancer melanocytes

scientific

Department of Pharmacology

University of Cambridge

44-1223-334032

CB2 1PD

Cambridge

Tennis Court Road

United Kingdom

multidrug transporters, steroids, structure/function

We study the molecular bases of the interactions of ATP-binding cassette transporters of human (e.g. ABCG1 and ABCG2) and bacterial origin (e.g., MsbA and LmrA) with chemotherapeutic drug and steroids and other lipids. We also study the potential physiological roles of multidrug transporters in lipid transport.

scientific

Dept. of Biochemistry

University of Oxford

+44 1865 275371

OX1 3QU

Oxford

South Parks Road

United Kingdom

membrane protein

membrane protein/lipid interactions biomolecular simulations

scientific

School of Chemistry

University of Southampton

++44 (0)2380 796161

SO17 1BJ

Southampton

University Road

United Kingdom

phospholipids, mass spectrometry, dynamic lipidomics

Mass spectrometry of lipids Dynamics of phospholipid synthesis Modelling lipid synthetic networks Lipid biomarkers in health and disease Synthesis, composition and function of endonuclear lipids Lung surfactant in health and disease Interaction between genotypic expression and diet in the regulation of the molecular species composition of cell lipids in vivo and in vitro Phospholipase-mediated cell signalling Membrane fusion Lipids in stem cell differentiation Lipids and nutrition Lipids in inflammation Oxidised phospholipids and cardiovascular disease

scientific

Centre for Equine and Animal Science

Writtle College

44 1245 424200

CM1 3RR

Chelmsford

Lordship Road

United Kingdom

poyunsaturated fatty acids, conjugated linoeic acids, immunity, health

Nutritional and biochemical role of fatty acids in the health and performance of farm and companion animals.

scientific

Centre for Equine and Animal Science

Writtle College

44 1245 424200

CM1 3RR

Chelmsford

Lordship Road

United Kingdom

poyunsaturated fatty acids, conjugated linoeic acids, immunity, health

Nutritional and biochemical role of fatty acids in the health and performance of farm and companion animals.

scientific

University of Iowa

Dept. of Molecular Physiology and Biophysics

1-319-335-7874

52246

Iowa City

6-530 Bowen Science Building

United States

multidrug resistance, sphingolipids, mitochondria

We study the interaction of sphingolipids and phospholipids with multidrug transporters in the yeast Saccharomyces cerevisiae. Transcription factors that modulate the expression of membrane transporters, often in the plasma membrane, have recently been found to also control the expression of genes involved in sphingolipid biosynthesis. Our goal is to understand the physiological rationale this coordinate control.

scientific

Indiana Umiversity -Purdue University Indianapolis

Indiana Umiversity -Purdue University Indianapolis

317-274-0593

46202

Indianapolis

723 W. Michigan St.

United States

yeast, sterol, ergosterol

yeast sterol biosynthesis

scientific

Institute of Molecular Medicine and Genetics

Medical College of Georgia

01-706-721-0699

GA 30912

Augusta

1120 15th Street

United States

Sphiogolipids, glycolipids, developmental biology, neurodegenerative diseases, neurochemistry

Analysis of glycolipid structure and metabolism in the nervous system, lipid signaling molecules and signal trnaduction, cell-cell recognition and adhesion, cell migration.

scientific

COBRE in Lipidomics & Pathobiology

Medical University of South Carolina

843-792-4323

29425

Charleston

PO Box 250509

United States

cell growth, cell death, cell aging, inflammation

Define the function of these fatty molecules in human disease, especially cancer, aging, neurologic disease and fungal pathogenesis.

scientific

COBRE in Lipidomics & Pathobiology

Medical University of South Carolina

843-792-4323

29425

Charleston

PO Box 250509

United States

cell growth, cell death, cell aging, inflammation

Define the function of these fatty molecules in human disease, especially cancer, aging, neurologic disease and fungal pathogenesis.

scientific

Center for Developmental Genetics

Stony Brook University

please fill in

11794

Stony Brook

438 CMM

United States

please fill in

please fill in

scientific

The Polt Group

The University of Arizona

++01-520-621-6322

AZ 85721

Tucson

Department of Chemistry

United States

PDMP, glycosphingolipid, sphingosine

Glycosphingolipids. Synthesis and structure elucidation. We are involved with the design and synthesis of glycosidase and glycosyltransferase inhibitors. We use Manduca sexta (tobacco horn worm) as a model system to explore the effects of GSLs on development. http://www.chem.arizona.edu/faculty/profile/profile.php?fid_call=polt

scientific

San Diego Supercomputer Center / LIPID MAPS Bioinformatics Core

University of California San Diego

858-822-3619

92093

La Jolla

9500 Gilman Drive, Mail Code 0412

United States

lipid classification, lipid database, lipid proteome, lipid pathways

The LIPID Metabolites and Pathways Strategy (LIPID MAPS) Consortium represents a multi-institutional effort to develop a detailed understanding of lipid structure and function. As part of this effort, we will develop ‘parts lists’ of lipid metabolites and assemble these into metabolic networks. These networks will then provide an infrastructure for subsequent modeling using quantitative data from LIPID MAPS experiments.

scientific

Nutrition and Genomics

USDA-Human Nutrition Research Center on Aging at Tufts University

++1-617-556-3102

02111

Boston

711 Washington St

United States

genetic polymorphisms, gene-diet interactions, perilipins,

The Nutrition and Genomics Laboratory has been pionnering the study of gene-diet interactions in the area of cardiovascular diseases, utilizing both genetic epidemiology approaches as well as controlled dietary intervention studies. This research involves the investigation of nutrient-gene interactions in large and diverse populations around the world with long-standing collaborations with investigators in Europe, Asia, Australia and the United States. More recently our interest has been focusing on the genetic factors involved in fat metabolism in the adypocite and more specifically the PATS family of genes. In addition, we seek to identify genes involved in longevity and healthy aging and to understand their regulation in response to dietary factors. For this research we use model organisms such as Drosophila and mouse. Further comprehension of the relation between genetic factors, nutrients and the rate of aging will provide better understanding of the pathology of age-related diseases and lead to improved strategies for their prevention.

scientific

Department of Biochemistry

Wake Forest University School of Medicine

please fill in

27157

Winston-Salem

Medicial Center Boulevard

United States

phospholipase D, bicelles, eicosanoids, lipoproteins, lipid kinases, virus assesmbly

The Department of Biochemistry (and affiliated faculty) at Wake Forest University has a working group of faculty members (about 14 faculty members) interested in the role of lipids in signal transduction, atherosclerosis, cancer, host defense, and inflammation. Techniques in use include thin-layer chromatography, HPLC, and mass spectrometry to identify phospholipid and fatty acid species, vesicle and solid-phase lipid binding assays, NMR spectroscopy and other biophysical approaches, coupled with site-directed mutagenesis, to study structural features of lipid:protein interactions, and computational modeling of signaling pathways involving lipids. Professor McPhail represents the lipid signaling working group on the departmental Development Committee, which advises the Chair on departmental development and policy.

scientific



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