Bibliographic Information Self-gelatinizable copolymer immobilized glucose biosensor based on prussian blue modified graphite electrode

Bibliographic Information
Probe beam deflection study on electrochemically controlled release of 5-fluorouracil. Huang, Hua; Liu, Changwei; Liu, Baifeng; Cheng, Guangjin; Dong, Shaojun. Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Peop. Rep. China. Electrochim. Acta (1998), 43(9), 999-1004. CODEN: ELCAAV ISSN: 0013-4686. Journal written in English. CAN 129:58691 AN 1998:302591 CAPLUS (Copyright 2001 ACS)
Abstract
The polypyrrole (PPy) film modified electrodes are used as an electro-releasing reservoir. The electrochem. controlled release of 5-fluorouracil (5-FU) from a PPy film modified electrode to aq. electrolytes was studied by the in situ probe beam deflection (PBD) method combined with cyclic voltammetry (CV) and chronoamperometry (CA). The PBD results reveal that the release of 5-FU from PPy film depends on the electrochem. redox process of the PPy film electrode. The released amt. was controlled by the redn. potential and is proportional to the thickness of the film. The exchange of 5-FU anions with Cl- on an open circuit is slow on the time scale of minutes, but the release of 5-FU anions can proceed quickly at -0.6 V (vs Ag/AgCl). The amt. of released 5-FU decreases with the time that the PPy film is soaked in aq. soln.
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Electrocatalytic oxidation of hydrazines at a 4-pyridylhydroquinone self-assembled platinum electrode and its application to amperometric detection in capillary electrophoresis. Niu, Li; You, Tianyan; Gui, John Y.; Wang, Erkang; Dong, Shaojun. Changchun Institute of Applied Chemistry, Laboratory of Electroanalytical Chemistry, Chinese Academy of Sciences, Changchun, Peop. Rep. China. J. Electroanal. Chem. (1998), 448(1), 79-86. CODEN: JECHES ISSN: 0368-1874. Journal written in English. CAN 129:73290 AN 1998:299817 CAPLUS (Copyright 2001 ACS)
Abstract
4-Pyridyl hydroquinone on a platinum electrode adsorbs through the pyridine nitrogen forming stable self-assembled layers. The electrocatalytic oxidn. of hydrazines was performed by the modified electrode. The overpotential of hydrazines was decreased markedly at the self-assembled monolayer (SAM) electrode. The mechanism of hydrazine oxidn. was also investigated. Amperometric detection of hydrazine under zero potential (vs. Ag|AgCl|sat. KCl) was exhibited by the SAM electrode used as an electrochem. detector in a flow system.
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Evaluation of charge transport in polyaniline-modified electrode by square wave voltammetry. Yang, Chunhe; Dong, Shaojun. Lab. Electroanalytical Chem., Changchun Inst. Appl. Chem., Chinese Acad. Sci., Changchun, Peop. Rep. China. Yingyong Huaxue (1998), 15(1), 68-70. CODEN: YIHUED ISSN: 1000-0518. Journal written in Chinese. CAN 128:217899 AN 1998:207651 CAPLUS (Copyright 2001 ACS)
Abstract
The charge transport rate in polyaniline (I) obtained through electropolymn. by cyclic voltammetry was evaluated by the square wave voltammetry, and the time of scale of voltammetry was defined by AC impedance technique. The charge transport in I is controlled by diffusion. The Dapp and k0 of the redox reaction are detd. by COOL algorithm by means of the fast scanning and sampling of the square wave voltammetry.
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A novel electrochemical method for simultaneously measuring diffusion coefficient and ion transference number. Zhou, Huafang; Shi, Zhong; Dong, Shaojun. Changchun Institute of Applied Chemistry, Laboratory of Electroanalytical Chemistry, Chinese Academy of Sciences, Jilin, Peop. Rep. China. J. Electroanal. Chem. (1998), 443(1), 1-3. CODEN: JECHES ISSN: 0368-1874. Journal written in English. CAN 128:263133 AN 1998:132821 CAPLUS (Copyright 2001 ACS)
Abstract
This paper introduces a new method to est. the diffusion coeff. and transference no. of a salt or an electroactive ion in a soln. with little or no supporting electrolyte. The above two parameters can be obtained from a single potential step expt. without previous knowledge of either one. It would appear that the method could also be used in the study of ion transport in a high viscosity solvent or a solid electrolyte.
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Electrochemical investigation of DNA adsorbed on conducting polymer modified electrode. Li, Zhuang; Wang, Hongda; Dong, Shaojun; Wang, Erkang. Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Peop. Rep. China. Anal. Sci. (1997), 13(Suppl., Asianalysis IV), 305-310. CODEN: ANSCEN ISSN: 0910-6340. Journal written in English. CAN 128:190057 AN 1998:102543 CAPLUS (Copyright 2001 ACS)
Abstract
Detection of DNA is a very important task in mol. biol. and biomedical fields. We have investigated cyclic voltammetry electrochem. behavior of double-stranded DNA and single-stranded DNA adsorbed on an electrode modified with polypyrrole conducting polymer in the presence of a cobalt complex. The possibility of using such electrode as gene detector is discussed.
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Studies of ferrocene derivative diffusion and heterogeneous kinetics in polymer electrolyte by using microelectrode voltammetry. Zhou, Huafang; Gu, Ningyu; Dong, Shaojun. Changchun Institute of Applied Chemistry, Laboratory of Electroanalytical Chemistry, Chinese Academy of Sciences, Changchun, Jilin, Peop. Rep. China. J. Electroanal. Chem. (1998), 441(1-2), 153-160. CODEN: JECHES ISSN: 0368-1874. Journal written in English. CAN 128:263123 AN 1998:101502 CAPLUS (Copyright 2001 ACS)
Abstract
The heterogeneous electron transfer rate consts. (ks) of seven ferrocene derivs. were estd. using cyclic voltammograms under mixed spherical/semi-infinite linear diffusion and steady-state voltammetry at a microdisk electrode in polymer electrolyte. The ks and diffusion coeff. (D) are both 100 to 1000-fold smaller in polymer solvent than in monomeric solvents, and the D and ks decrease with increasing polymer chain length. The results conform to the difference of viscosity (h) or relaxation time (tL) for these different solvents. The ks and D increase with increasing temp., and the activation barriers of the electrode reaction are obtained. The influences of the substituting group in the ferrocene ring on ks and D are discussed. The ks are proportional to the D of the ferrocene derivs., which indicates that solvent dynamics control the electrode reaction.
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A study on the diffusion of quinhydrone in polymer electrolyte. Dong, Shaojun; Gu, Ningyu; Zhou, Huafang. Changchun Institute of Applied Chemistry, Laboratory of Electroanalytical Chemistry, Chinese Academy of Sciences, Changchun, Jilin, Peop. Rep. China. J. Electroanal. Chem. (1998), 441(1-2), 95-101. CODEN: JECHES ISSN: 0368-1874. Journal written in English. CAN 128:249778 AN 1998:101492 CAPLUS (Copyright 2001 ACS)
Abstract
The diffusion coeffs. (D) of quinhydrone were estd. in polymer electrolytes by using non-steady-state chronoamperometry and steady-state current voltammetry. The D values have been estd. in polyethylene glycol (PEG) contg. different concns., and cations of supporting electrolytes, and in different solvents over a range of temps. The dependencies of electroactive probe diffusion coeffs. on temp., supporting electrolyte concn. and polymer chain length are discussed. The results show that D increases with increasing temp. and decreasing concn. of supporting electrolyte. The diffusion coeff. depends strongly on the length of polymer chain and decreases sharply with increasing polymer chain length. The contribution of electron self-exchange has been explored and it seems to be negligible here.
Bibliographic Information
Glucose biosensor based on a terpolymer gel from grafting 4-vinylpyridine and acrylamide on poly(vinyl alcohol). Kou, Wenpeng; Li, Bin; Deng, Qing; Cheng, Guangjin; Dong, Shaojun. Laboratory Electroanalytical Chemistry, Chinese Academy Sciences, Changchun Institute Applied Chemistry, Changchun, Peop. Rep. China. Fenxi Huaxue (1998), 26(1), 73-76. CODEN: FHHHDT ISSN: 0253-3820. Journal written in Chinese. CAN 128:215105 AN 1998:90314 CAPLUS (Copyright 2001 ACS)
Abstract
A new terpolymer was synthesized by grafting 4-vinylpyridine and acrylamide on the backbone of poly(vinyl alc.). A glucose biosensor based on immobilization of glucose oxidase in the terpolymer gel is described. The enzyme electrode responds to glucose in 7.apprx.10 s and gives a c.d. of 480 nA/mm2 for 1 mmol/L glucose at 0.65 V (vs. Ag/AgCl). A linear calibration curve is obtained over the range 1´10-5.apprx.5.0´10-3 mol/L and the enzyme electrode retains 85% of initial response after a sue period of two months.
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Synthesis of a self-gelatinizable grafting copolymer of poly(vinyl alcohol) for construction of an amperometric peroxidase electrode. Li, Bin; Niu, Li; Kou, Wenpeng; Deng, Qing; Cheng, Guangjin; Dong, Shaojun. Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Peop. Rep. China. Anal. Biochem. (1998), 256(1), 130-132. CODEN: ANBCA2 ISSN: 0003-2697. Journal written in English. CAN 128:254709 AN 1998:84824 CAPLUS (Copyright 2001 ACS)
Abstract
This article reports the synthesis of PVA-graft-PVP copolymer for the first time. This type of copolymer is self-gelatinizable and can firmly adhere to the electrode surface. Utilizing these merits, the authors established a more temperate method for construction of biosensors by simply syringing the copolymer-enzyme aq. soln. to the electrode surface and allowing water to evap.
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Cobalt octaethyltetrapyrazinoporphyrazine: spectroscopy, and electrocatalytic and kinetic study of dioxygen reduction at a glassy carbon electrode. Dong, Shaojun; Liu, Baifeng; Liu, Junli; Kobayashi, Nagao. Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Jilin, Peop. Rep. China. J. Porphyrins Phthalocyanines (1997), 1(4), 333-340. CODEN: JPPHFZ ISSN: 1088-4246. Journal written in English. CAN 128:209995 AN 1998:68100 CAPLUS (Copyright 2001 ACS)
Abstract
Cobalt octaethyltetrapyrazinoporphyrazine was synthesized and characterized by electronic absorption and magnetic CD spectroscopy. These spectroscopies and MO calcns. within the framework of the PPP approxn. revealed that the effective p-conjugation system of octaethyltetrapyrazinoporphyrazine is smaller than phthalocyanines. For the 1st time, the electrochem. behavior of cobalt octaethyltetrapyrazinoporphyrazine irreversibly adsorbed onto a glassy carbon electrode as a surface modifier is reported. The cobalt deriv./glassy carbon electrode showed catalytic activity for oxygen redn. Surprisingly, the peak potentials for oxygen redn. at this electrode coincide well with those where CoII is reduced to CoI over the whole pH range, indicating that the active species for dioxygen redn. is CoI rather than the CoII species. A possible mechanism for the electrocatalysis of oxygen is discussed.
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Electrochemical studies of a lanthanide heteropoly tungstate/molybdate complex in polypyrrole film electrode and its electrocatalytic reduction of bromate. Dong, Shaojun; Cheng, Long; Zhang, Xiumei. Laboratory of Electroanalytical Chemistry, Chinese Academy of Sciences, Changchun Institute of Applied Chemistry, Changchun, Peop. Rep. China. Electrochim. Acta (1997), Volume Date 1998, 43(5-6), 563-568. CODEN: ELCAAV ISSN: 0013-4686. Journal written in English. CAN 128:160202 AN 1998:11173 CAPLUS (Copyright 2001 ACS)
Abstract
An electrode modified with a polybasic lanthanide heteropoly tungstate/molybdate complex K10H3[Nd(SiMo7W4O39)2] entrapped into polypyrrole (PPy) film, denoted as Nd(SiMo7W4)2PPy, exhibits three couples of two-electron redox waves in pH 1-5 buffer solns. The redox waves are surface-controlled at lower scan rates and diffusion-controlled at higher scan rates. The effects of pH on the electrochem. behavior of Nd(SiMo7W4)2 in PPy film were studied in detail and compared with that of Nd(SiMo7W4)2 in aq. soln. The various charge states of PPy during its redox process have peculiar effects on the relation between pH and formal potentials of Nd(SiMo7W4)2-PPy at different acidities. The Nd(SiMo7W4)2-PPy cme can remarkably catalyze the electrochem. redn. of bromate with good stability. The Nd(SiMo7W4)2-PPy cme can remarkably catalyze the electrochem. redn. of bromate with good stability.
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Advances in solid polymer electrolytes. Ding, Liming; Dong, Shaojun; Wang, Erkang. Laboratory of Electroanalytical Chemistry, Chinese Academy of Sciences, Changchun, Peop. Rep. China. Dianhuaxue (1997), 3(4), 349-361. CODEN: DIANFX ISSN: 1006-3471. Journal; General Review written in Chinese. CAN 128:35336 AN 1998:4111 CAPLUS (Copyright 2001 ACS)
Abstract
A review with 84 refs. on formation, classification, applications and ion-transport mechanism of solid polymer electrolytes.
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Unidirectional current flow of reversible redox couples on a MoO3 film-modified microelectrode. Wang, Baoxing; Li, Xiao-yuan; Dong, Shaojun. Dep. Chemistry, Hong Kong Univ. Sci. and Technology, Kowloon, Hong Kong. J. Electroanal. Chem. (1997), 435(1-2), 23-30. CODEN: JECHES ISSN: 0368-1874. Journal written in English. CAN 128:146634 AN 1997:810837 CAPLUS (Copyright 2001 ACS)
Abstract
The authors have studied the reactivity of the molybdenum oxide film toward some std. redox systems (e.g., ferrocene (Fc) and its derivs.) and obsd. a few interesting phenomena. The electrochem. behavior of Fc and its derivs. at the oxide-modified carbon fiber (CF) microelectrode differs from that at a bare CF microelectrode. The cond. of the molybdenum oxide film is seriously affected by the range and the direction of the potential scan, which influences the electrochem. behavior of these redox systems at the film electrode. If the cycling potential is more pos. than the redn. potential of the molybdenum oxide film, the redn. and oxidn. peak currents of Fc and its derivs. could not be obsd. The molybdenum oxide film on a microelectrode surface cannot transfer electrons between the surface of the electrode and Fc or its derivs. due to the existence of a high resistance between the interface in these potential ranges. However, if the lower limit of the scan potential was extended to a potential more neg. than the redn. peak potential of the film, the oxidn. peak of Fc or its derivs. appeared at about the potential relative to Eo of Fc or its derivs. on the bare electrode, and the peak current is proportional to the concn. of these couples in the electrolyte. To the authors' surprise, the peak height on the modified electrode is much larger than that on the bare CF microelectrode under the same conditions in the range of low concn. of these couples, and the oxidn. peak potential of these couples is more neg. than that on the bare CF microelectrode. From the exptl. observation, probably these redox couples undergo an interaction with the redn. state of the molybdenum oxide film. The new phenomena that the authors obsd. were explained by using this interaction.
Bibliographic Information
Self-assembly of the pre-formed inclusion complexes between cyclodextrins and alkanethiols on gold electrodes. Yan, Juchao; Dong, Shaojun. Changchun Institute of Applied Chemistry, and National Analysis and Research Center of Electrochemistry and Spectroscopy, Laboratory of Electroanalytical Chemistry, Chinese Academy of Sciences, Changchun, 130022, Peop. Rep. China. J. Electroanal. Chem. (1997), 440(1-2), 229-238. CODEN: JECHES ISSN: 0368-1874. Journal written in English. CAN 128:120891 AN 1997:790436 CAPLUS (Copyright 2001 ACS)
Abstract
A new kind of self-assembled monolayer (SAM) formed in aq. soln. through the pre-formed inclusion complexes (abbreviated CD×Cn) between a-, b-cyclodextrins (CDs) and alkanethiols (CH3(CH2)n-1SH, n=10, 14 and 18) was prepd. successfully on gold electrodes. High-resoln. 1H NMR was used to confirm the formation of CD×Cn. XPS, cyclic voltammetry and chronoamperometry were used to characterize the resulting SAMs (denoted as MCD×Cn). It was found that MCD×Cn were more stable against repeated potential cycling in 0.5M H2SO4 than SAMs of CH3(CH2)n-1SH (denoted as MCn), with a relative sequence of Mb-CD×Cn>Ma-CD×Cn>MCn. In addn., an order of blocking the electron transfer between gold electrodes and redox couples (both Fe(CN)63- and Ru(NH3)63+) in soln., MCD×C10>MCD×C14>MCD×C18, was obsd. A plausible explanation is provided to elucidate some of the observations.
Bibliographic Information
Identification of o-phenylenediamine polymerization product catalyzed by cytochrome c. Zhu, Yimin; Li, Jinghong; Liu, Zhiming; Cheng, Guangjin; Dong, Shaojun; Wang, Erkang. Changchun, Changchun Institute of Applied Chemistry, Laboratory of Electroanalytical Chemistry, Chinese Academy of Sciences, Jilin 130022, Peop. Rep. China. J. Mol. Catal. B: Enzym. (1998), 4(1-2), 33-39. CODEN: JMCEF8 ISSN: 1381-1177. Journal written in English. CAN 128:189817 AN 1997:759200 CAPLUS (Copyright 2001 ACS)
Abstract
The hydrogen peroxide (H2O2) and cytochrome c-dependent oxidn. of o-phenylenediamine (o-PD) was investigated by spectrophotometry and electrochem. The results indicated that o-PD underwent facile catalytic oxidn. in the presence of cytochrome c, and that the degrdn. of cytochrome c by hydrogen peroxide can also be partly prevented in the presence of o-PD. The hydroxyl radical scavengers (mannitol and sodium benzoate) and oxo-heme species scavenger (uric acid) do not inhibit the oxidn., which implies that the hydroxylation of o-PD may not be involved in its oxidn. Combining with the results of the mass spectrum, elemental anal., NMR and Fourier transform IR spectrum of the isolated product, a conceivable structure of the product was suggested.
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Formation of self-assembled monolayers on gold electrodes with inclusion complexes of cyclodextrins and viologens. Yan, Juchao; Dong, Shaojun; Li, Jinghong; Chen, Wenqi. Laboratory of Electroanalytical Chemistry, Changchun, Peop. Rep. China. J. Electrochem. Soc. (1997), 144(11), 3858-3865. CODEN: JESOAN ISSN: 0013-4651. Journal written in English. CAN 128:81242 AN 1997:749405 CAPLUS (Copyright 2001 ACS)
Abstract
A novel kind of electroactive self-assembled monolayer (SAM) was successfully prepd. through the following procedure: (1) formation of inclusion complexes (denoted as CD/C8VC10SH) between N-(n-octyl)-N'-(10-mercaptodecyl)-4,4'-bipyridinium dibromide (C8VC10SH) and a-, b-cyclodextrin (CD) under a mild condition; (2) spontaneous formation of SAM of CD/C8VC10SH on gold electrodes at room temp. High-resoln. 1H-NMR spectrum was used to confirm the formation of CD/C8VC10SH. Cyclic voltammetry was used to characterize the redox behavior of the resulting monolayers and chronoamperometry and electrochem. impedance spectroscopy to characterize their electron transfer kinetics. The redox sites in SAM of CD/C8VC10SH are effectively dild., with a larger electron transfer rate const. than that of SAM of C8VC10SH.
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The effect of substrate and solvent properties on the response of an organic phase tyrosinase electrode. Deng, Qing; Dong, Shaojun. Changchun Institute of Applied Chemistry, Laboratory of Electroanalytical Chemistry, Chinese Academy of Sciences, Changchun, Peop. Rep. China. J. Electroanal. Chem. (1997), 435(1-2), 11-15. CODEN: JECHES ISSN: 0368-1874. Journal written in English. CAN 128:151246 AN 1997:734315 CAPLUS (Copyright 2001 ACS)
Abstract
The responses of a cryohydrogel tyrosinase enzyme electrode to 4 substrates in 3 pure water immiscible org. solvents were investigated. Kinetic parameters, the max. kinetic current, Imax, the apparent Michaelis-Menten const., Kappm, and Imax/Kappm, were calcd. The Imax/Kappm value was taken as an indicator of the catalytic efficiency of the sensor. The effect of the substrate hydrophobicity on Imax/Kappm and response time of the sensor were discussed. The effects of both hydrophobicity and dielec. const. of the org. solvent on the catalytic efficiency of the enzyme in the org. phase were studied.
Bibliographic Information
Scanning tunnelling microscopy observation of cytochrome-c denaturation induced by bromopyrogal red on highly oriented pyrolytic graphite. Zhang, Bailin; Zhu, Yimin; Dong, Shaojun; Wang, Erkang. Lab. Electroanalytical Chem., Changchun Inst. Applied Chem., Chinese Academy Sci., Changchun, Peop. Rep. China. Int. J. Biol. Macromol. (1997), 21(3), 251-261. CODEN: IJBMDR ISSN: 0141-8130. Journal written in English. CAN 128:19846 AN 1997:708811 CAPLUS (Copyright 2001 ACS)
Abstract
The denaturation of cytochrome-c (cyt-c) induced by bromopyrogal red (BPR) was studied by scanning tunnelling microscopy (STM) on the electrochem. pretreated highly oriented pyrolytic graphite (HOPG) surface. STM images reveal that denatured cyt-c mols. exist in variable states including aggregates, globular compact, partially unfolded and combined with BPR mol. The apparently low image contrast of denatured cyt-c obsd. in this expt. comparing to that of native cyt-c mols., and the relative low image contrast of the unfolded part comparing with the compact globular part, are ascribed to the unfavorable tunnelling paths for the conformational variations of denatured cyt-c mols.
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Interfacial characteristics of the self-assembly system of poly-L-lysine|3-mercaptopropionic acid|gold electrode. Li, Jinghong; Ding, Lin; Wang, Erkang; Dong, Shaojun. Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Peop. Rep. China. J. Electroanal. Chem. (1997), 431(2), 227-230. CODEN: JECHES ISSN: 0368-1874. Journal written in English. CAN 127:363485 AN 1997:663849 CAPLUS (Copyright 2001 ACS)
Abstract
The interfacial characteristics of poly-L-lysine (PL) attached on self-assembled monolayers (SAMs) of 3-mercaptopropionic acid (MPA) were studied by an electrochem. method. PL|MPA layer inhibited partly the diffusion process of redox species in soln., and the electrode surface behaved like a microelectrode array. Its permeation effect was also strongly affected by Mg2+. The more Mg2+ ions were added into the electrolyte soln., the greater the difficulty with which the electron transfer of potassium ferricyanide took place. The three different conformations of PL on the electrode surface had different influences on the electron transfer processes of ferricyanide. PL in random coil state hindered most strongly the electron transfer behavior of ferricyanide, while the a-helical PL had nearly no effect and the effect of the b-sheet state PL was intermediate of these.
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The electrochemical study of oxidation-reduction properties of horseradish peroxidase. Li, Jinghong; Dong, Shaojun. Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Peop. Rep. China. J. Electroanal. Chem. (1997), 431(1), 19-22. CODEN: JECHES ISSN: 0368-1874. Journal written in English. CAN 127:343271 AN 1997:663822 CAPLUS (Copyright 2001 ACS)
Abstract
Oxidn.-redn. properties of horseradish peroxidase (HRP) have been investigated by using direct electrochem. methods. Two successive sepd. distinct one-electron processes of HRP were obtained and the related physiol. processes were described. The monolayer coverage of HRP at the electrode surface is about 50 pmol/cm2. UV-Vis spectrophotometry and stable amperometry prove that the enzyme electrode possesses catalytic activity for H2O2 in the absence of a mediator and it might offer an opportunity to build the third generation of biosensors for analytes, such as H2O2, glucose and cholesterol etc.