Curriculum vitae prof. Amarnath bhaduri

CURRICULUM VITAE

pROF. AMARNATH BHADURI, D.SC, F.A.SC, F.N.A • INDIAN Institute of Chemical Biology. • 4, Raja S.C. Mullick Road, Jadavpur, • CALCUTTA-700032. • India. •
Phone [phone] Extn: 102, 164(off). 91-33-4178424 (Resi). • Fax [phone] • E-mail: [e-mail]; DOB: 12TH nOVEMBER, 1935•
NAME Prof. AMARNATH BHADURI	DESIGNATION INSA Scientist and Former Director, IICB

ACADEMIC RECORDS

INSTITUTION AND LOCATION	DEGREE (if applicable)	YEAR(s)	FIELD OF STUDY
University of Calcutta, Calcutta, India University of Calcutta, Calcutta, India University of Michigan, USA Harvard Medical School, USA	B.Sc. (Hons) M.Sc D.Sc Research Fellow	1955 1958 1963 1963-66	Chemistry Applied Chemistry Biochemistry Biochemistry

POSITIONS HELD

INSTITUTION AND LOCATION	POSITION	YEAR(s)
Jadavpur University, Calcutta, India Jadavpur University, Calcutta, India Jadavpur University, Calcutta, India Roche Institute of Molecular Biology, USA Indian Institute of Chemical Biology, Calcutta, India Indian Institute of Chemical Biology, Calcutta, India Indian Institute of Chemical Biology, Calcutta, India Indian Institute of Chemical Biology, Calcutta, India	Lecturer Reader Professor Visiting Scientist [Sabbatical] Scientist-in-Director’s Rank Director Emeritus Scientist,CSIR Hon. Sct., In. Natl. Sci. Acad.	1966-71 1971-76 1976-86 1975-76 1986-89 1990-1995 1996-2000 2001 onwards

HONOURS

Fellow Fellow D.Sc [Hon Causa] President

Indian National Science Academy Indian Academy of Sciences Burdwan University Society of Biological Chemists, India

1986 1988 1995 1998-2000

AWARDS

(i)Bhatnagar Award in Biological Science (1979), one of the most prestigious awards in Indian Sciences.

(ii)Prof.B.C.Guha Oration Lecture (1991), University of Calcutta, India

(iii)Prof. J.C.Bose Gold Medal (1995), Indian National Science Academy

(iv)Dr.S.C.Roy Memorial Medal(1996),Calcutta University

(v)Jawaharlal Nehru Birth Centenary Lecture (1997), Indian National Science Academy

(v)Dr.K.P.Basu Memorial Oration Award (1998), Jadavpur University

(vi)Prof.S.P.Raychowdhury Oration Award (1998), Society of Cell Biology, India

(viii)Dr.B.N.Singh Memorial Oration Award (1999), Indian Society of Parasitology.

(viii)Dr.Rajendra Prasad Oration Award (2000), Patna Medical Sciences, I.C.M.R

(ix)J.B.Chatterjee Memorial Oration(2001), Calcutta School of Tropical Medicine

(x) Foundation Day Oration Award (2001) Institute of Microbial Technology, CSIR.

(xi) Prof. J.C. Ghosh Oration Award (2001) Science Association of Bengal.

(xii)B.N.Ghosh Medal(2001),Calcutta University

EXPERT MEMBER [INTERNATIONAL]

Co-opted Member: Steering Committee on the Immunology and Biochemistry of Leishmania Expert Advisory Panel for Parasitic Diseases [Leishmania Sec]

1989 1994-97, 1998-2000, 2001-2003

World Health Organisation, Geneva World Health Organisation, Geneva

EXPERT MEMBER [NATIONAL]

Biochemistry Research funding Committee Biochemistry and Biophysical Research funding Committee Committee of Emeritus Scientist Chairman, Research Advisory Council Member, Research Council of National Laboratories (i)Centre for Biochemical Technology, New Delhi, India (ii)Institute of Microbial Technology, Chandigarh (iii)Central drug Research Institute, Lucknow (iv)Centre for Cellular and Molecular Biology, Hyderabad Member, Drug and Pharmaceuticals promotion committee Member, Scientific Advisory Committee Member, Promotion and Assessment committee Member, Governing Body Chairman, Scientific Advisory Committee Several Other Similar Committees

1986-89, 1989-92 1988-91, 1991-1994 1988-91 1986-89 1989-92, 1992-95 1994-96 1992-95 1994-96 1994-98 1994-97, 2000-2002 1994-98, 1998-2002 1994-till date 2001-2004 [Not Detailed]

Council of Scientific and Industrial Research, New Delhi, India Dept. of Science and Technology, Govt. of India Council of Scientific and Industrial Research, New Delhi, India Centre for Biochemical Technology, New Delhi, India Department of Science and Technology, New Delhi, India Indian Council of Mediacal Research, New Delhi, India Indian Institue of Science, Bangalore, India National Institute of Immunology, New Delhi, India National Institute of Immunology, New Delhi, India

OTHER ACADEMIC ACTIVITIES

(a) Member, Executive Council, Indian Academy of Science, Bangalore, India (1992-95)

(b) Member, Executive Council, Indian National Academy of Science (1997-1999)

(c) Member, Executive Committee, Dept of Science and Technology, West Bengal (1994-98)

(d)Member, National Organizing Committee, XVI IUBMB Congress, New Delhi (1994)

(e) Member, Executive Council of Syndicate, University of Calcutta (1991-94)

(f) Numerous invited talks in last twenty years in International and National Congresses, Symposia etc.

ADMINISTRATIVE EXPERIENCE

Wide administrative experience in scientific and technological areas:

(a) As Director of a premier National Laboratory since 1990, was involved in planning and time-targeted execution of scientific projects of the Institute. Scientific manpower development, budgetary resource mobilization and its proper utilization also had been part of regular administrative activity.

(b) As National Coordinator of the UNDP project $1.35 million entitled, “Molecular Biology and Biotechnology of Leishmania Parasite” (1998-95) gave direction and shape to this coordinated scientific programme. The institute is now internationally known as a major center of Leishmania research.

(c)Organized several Interbnational Scientific Symposia in this Institute. Was closely involved in organizing the XVIth Congress of International Union of Biochemistry and Molecular Biology at New Delhi (Sept. 1994) and International Congress on Leishmania (Leishmania-II) in Creete, Greece (2001).

(d) Made extensive lecture and study tours in UK, USA, Germany, Australia, China, Japan and many other parts of the world as a delegate of different Indian Scientific Organizations and in various other individual capacities.

RESEARCH EXPERIENCE

(a) Extensive research experience in most aspects of enzymology and metabolic biochemistry.

(b) Major experience in Parasite Biochemistry, Parasitology, Drug development.

(c) Guide of 21 Ph.D students.

LIST OF PUBLICATIONS

Annexure-I

RESEARCH CONTRIBUTIONS [SUMMARY]

A.Enzymology

UDPgalactose 4-epimerase, an obligatory enzyme of galactose metabolism has emerged on the basis of its mechanism as the prototype of a new class at oxidoreductase. Unlike in case of classical dehydrogenases or oxidoreductases, the enzyme bound pyridine nucleotide [NAD] for this class of enzyme is used as a true cofactor and not as a cosubstrate. Employing all current techniques of biochemistry and biophysics and exploiting the fluorescent pyridine moiety as the monitor of the active site, Prof. Bhaduri has been able to work out in molecular detail the chemical architecture of the active site of this enzyme. Molecular designing of stacked and quenched uridine nucleotide fluorophores as substrate analogues is a highlight of this work. His recent work in progress indicates the enzyme to be a bifunctional catalyst. Coupled with his work on the assembly pathway of this homodimeric enzyme, his work constitutes a major advancement in the molecular enzymology of this class of enzymes [References 8-39].

B. Parasite Biology

Prof. Bhaduri’s extensive biochemical and cell biological analysis of metabolic networks of Leishmania donovani, the protozoal pathogen for Kala-azar, have provided new leads for drug development and for establishing the role of Ca2+ as a crucial biomodulator in pathogenic transformation of the parasite. The pyrimidine biosynthetic pathway and the respiratory chain of the parasite have been shown to have unique enzymological features, that can be exploited for rational drug designing. Discovery and characterization of a high affinity, calmodulin-regulated plasma-membrane Ca2+-ATPase acting as an extrusion pump for Ca2+; rapid release of Ca²⁺ from internal pool on upward temperature shift and discovery of Ca2+-CaM protein phosphatase have firmly established the role of Ca²⁺ as a signal molecule in morphogenetic transformation. Parallely, he has shown the Leishmania plasma membrane Mg²⁺-ATPase to be a H⁺-K⁺ antiporter that is directly involved in K⁺ accumulation and generation of H⁺ gradient.

In short, the work of Prof. Bhaduri has thrown important new lights on several aspects of Leishmania biology that will have wide implications to understand its life cycle and for drug development [References 40-57].

C. Black Tea

This is a recent area of research for Prof. Bhaduri. Two enzymes involved in flavor and color generation for black tea has been characterized. Black tea and theaflavin, its characteristic constituent have been shown to be powerful scavengers of reactive oxygen and nitrogen species. The chemopreventive role of Black tea in apoptosis and cell proliferation is being explored intensively [References 58-61].

Annexure-I: LIST OF PUBLICATIONS

I.Publications as doctoral candidate [Univ. of Michigan (1960-63)]

1. 
   Incorporation of radioactive citrate into fatty acids. A. Bhaduri and P.A.Srere (1962) Biochem. Biophys. Acta 59,487-89.
   
2. 
   The incorporation of citrate carbon into fatty acids. A. Bhaduri and P.A.Srere (1963) Biochem. Biophys. Acta 70,221-30.
   
3. 
   The citrate-cleavage enzyme: Stereospecificity with Citryl CoA as substrate. A. Bhaduri and P.A.Srere (1964) J.Biol.Chem. 239, 714-18.
   
4. 
   Mitochondrial stimulation of fatty acid biosynthesis. A. Bhaduri and P.A.Srere (1964) J.Biol.Chem. 239, 1357-63.
   

II. Publications as postdoctoral fellow [Harvard Mediacl School (1963-65)]

5. 
   Enhanced fluorescence of an epimerase elicited by 5 uridine nucleotides. A. Bhaduri, A. Christensen and H.M.Kalckar (1965) Biochem. Biophys. Res. Commun. 21, 624-31.
   
6. 
   Molecular transitions in an induced enzyme system. C. Crevling, A. Bhaduri and H.M.Kalckar (1965) Biochem. Biophys. Res. Commun. 21, 631-37.
   

III. Publications as Visiting Scientist at Roche Institute of Molecular Biology

7. 
   Simultaneous reconstitution of Escherechia coli membrane vesicles with D-lactose and D-amino acid dehydrogenase. H.Halder, P. Olseriwski, C. Walsh, G. Kaczorowski, A. Bhaduri and R. Kaback (1982) Biochemistry 21, 4590-96.
   

IV. Publications as independent investigator (1970 onwards)
[A] Molecular Enzymology and related areas:

8.Nucleotide inhibition of UDPglucose 4-epimerase from Saccharomyces fragilis and from goat liver. D.K.Pal and A.N.Bhaduri (1971) Biochem. Biophys. Acta 250, 588-91.

9.Galactose 6-phosphate dhydrogenase: A new enzyme from mammalian liver. M.Ray, D.K.Pal and A. Bhaduri (1972) FEBS Letts. 25, 239-41.

10. Two forms of UDPglucose 4-epimerase from mammalian liver. M.Ray and A. Bhaduri (1973) Biochem. Biophys. Acta. 320, 129-34.

11.UDPglucose 4-epimerase from Saccharomyces fragilis: Interaction with sugar phosphates at an effector site. M.Ray and A.Bhaduri (1974) Biochem. Biophys. Res. Commun. 69, 1081-89.

12. Galactose 6-phosphate dehydrogenase purification and partial characterization. M.Ray and A. Bahduri. (1975) J.Biol. Chem. 250, 3395-3601.

13. UDPglucose 4-epimerase from Saccharomyces fragilis: Allosteric kinetics with UDPglucose as substrate. M.Ray and A. Bhaduri (1975) J.Biol. Chem. 250, 4373-75.

14. UDPglucose 4-epimerase from Saccharomyces fragilis: Desensitization with heat. M.Ray and A. Bhaduri (1975) Biochem. Biophys. Res. Commun. 67, 877-82.

15. Purification and partial characterization of UDPglucose 4-epimearse from goat brain. M.Chandra and A. Bhaduri (1976) J. Neurochem. 27, 641-42.

16. On the interaction of sugar phosphatase and cations with UDPglucose 4-epimerase from Saccharomyces fragilis. M.Ray, K.Kar and A. Bhaduri (1976) Ind. J. Biochem. Biophys. 13, 311-15.

17. UDPglucose 4-epimerase from Saccharomyces fragilis: Inactivation by heat and reconstitution of the inactive enzyme. M.Ray and A. Bhaduri (1976) Eur. J. Biochem. 70, 319-23.

18. UDPglucose 4-epimerase from Saccharomyces fragilis: Activity and fluorescence of native enzyme in relation to sulfydryl groups. M.Ray, K.Kar and A. Bhaduri (1978) Ind. J. Biochem. Biophys. 417-19.

19. UDPglucose 4-epimerase from Saccharomyces fragilis: Involvement of sulfhydryl groups at the active site. M.Ray, K.Kar and A. Bhaduri (1978) Biochem. Biophys. Acta. 526, 635-39.

20. UDPglucose 4-epimerase from Saccharomyces fragilis: Asymmetry in allosteric properties leads to unidirectional catalysis. M.Ray and A. Bhaduri (1978) Biochem. Biophys. Res. Commun. 85, 242-48.

21. Presence of two conformationally vicinal sulfhydryl groups at the active site at UDPglucose 4-epimerase from Saccharomyces fragilis. M.Ray and A. Bhaduri (1980) J. Biol. Chem. 255, 10777-81.

22. Fluorescence properties of reconstituted forms of UDPglucose 4-epimerase from Saccharomyces fragilis. M.Ray and A. Bhaduri (1980) J.Biol. Chem. 255, 10782-86.

23. Interaction of 8-Anilino-1-napthalene sulphonic acid with UDPglucose 4-epimerase from Saccharomyces fragilis. A.Samanta and A. Bhaduri (1982) Ind. J. Biochem. Biophys. 19, 320-23.

24. The presence of elements of a dinucleotide fold in UDPglucose 4-epimerase from Saccharomyces fragilis. A.Samanta and A. Bhaduri (1982) Biochem. Biophys. Acta. 19, 129-32.

25. Characterization of pyridine nucleotide binding site of UDPglucose 4-epimerase from Saccharomyces fragilis. A. Samanta and A. Bhaduri (1983) J. Biol. Chem. 258, 11118-11122.

26. Purification and characterization of UDPglucose 4-epimerase from ascites tumor cells. S. Dutta, M. Ray and A. Bhaduri (1985) J. Biosciences 9, 59-70.

27. Biochemical investigations on galactose-induced toxicity in gal mutants of Escherichia coli. N. Rout and A. Bhaduri (1985) J. Biosciences 9, 71-81.

28. Presence of an arginine residue at the subtrate binding site of UDPglucose 4-epimerase from Saccharomyces fragilis. S. Mukherjee and A. Bhaduri (1986) J. Biol. Chem. 261, 4519-23.

29. Substrate induced suicide inhibition of UDPglucose 4-epimerase during catalytic cycle. S. Dutta, M. Ray and A. Bhaduri (1986) J. Protein Chem. 5, 291-292.

30. Conformational vicinal thiols of UDPglucose 4-epimerase from Saccharomyces fragilis: Selective roles in maintaining coenzyme fluorescence and activity. H. Bhattacharjee and A. Bhaduri (1988) Ind. J. Biochem. Biophys. 25, 149-54 [By Invitation].

31. Metabolic analysis of galactose toxicity in Escherichia coli with 2-dioxygalactose as the probe. N. Rout and A. Bhaduri (1991) Ind. J. Biochem. Biophys. 28, 541-45.

32. Scanning of hydrophobic subsites in the active site of UDPglucose 4-epimerase with synthesized fluorescent substrate derivates. S. Ray, E. Ali and A. Bhaduri (1992) Ind. J. Biochem. Biophys. 29, 209-13 [By Invitation].

33. Synthesis and lysis of format by immobilized cells of Escherichia coli. R. Nandi, P.K. Bhattacharyya, A. Bhaduri and S. Sengupta (1992) Biotechnology and Bioengineering 39, 775-780.

34. An epimerase histidine residue at the active site of UDPglucose 4-epimerase from Saccharomyces fragilis. S. Mukherjee and A. Bhaduri (1992) J. Biol. Chem. 267, 11709-13.

35. Distinct functional roles of two conformationally vicinal thiols at the active site of UDPglucose 4-epimerase from Saccharomyces fragilis. H. Bhattacharjee and A. Bhaduri (1992) J. Biol. Chem. 267, 11714-20.

36. Two trptophans at the active site of UDPglucose 4-epimerase from Saccharomyces fragilis. S. Ray, S. Mukherjee and A. Bhaduri (1995) J. Biol. Chem. 270, 11383-90.

37. UDPglucose 4-epimerase from Kluveromyces fragilis: reconstitution and assembly of holoenzyme S. Majumdar, H. Bhattacharjee and A. Bhaduri (1998) Eur. J. Biochem. 257,427-33.

38. Synthesis and characterization of stacked and quenched nucleotide uridine fluorophores. G. Dhar and A. Bhaduri (1999) J. Biol. Chem. 274, 14568-72.

39. An arginine residue is essential for binding and stretching of substrate for UDPgalactose 4-epimerase from E. coli: Use of stacked and quenched uridine nucleotide fluorophore as probe. U. Bhattacharya, G. Dhar and A. Bhaduri (1999) J. Biol. Chem. 274, 14573-78.
[B] Parasite Biochemistry and Biotechnology

40. Antileishmanial activity of mycobacillin. T. Chatterjee, D.K.Ghosh and A. Bhaduri (1977) J. Antibiotics 30, 262-67.

41. Mechanism of action of Amphotericin B as an antileishmanial agent. A.K.Saha, T. Mukherjee and A. Bhaduri (1986) Mol. Biochem. Parasitol. 19, 195-200.

42. Antileishmanial activity of methylglyoxal. S. Sarkar and A. Bhaduri (1986) Ind. J. Exp. Biol. 24, 296-98.

43. Preparation and characterization of ghosts from Leishmania donovani promastigotes. S. Sarkar and A. Bhaduri (1986) Current Trends in Life Sciences. XIII Biomembrane Structure, Biogenesis and Transport. Ed. L. Packer and C. Rajamanickam (1987) pp 305-310.

44. Aspartate Transcarbamylase from Leishmania donovani promastigotes. A discrete nonregulatory enzyme as a potential chemotherapeutic site. T. Mukherjee, M. Ray and A. Bhaduri (1988) J. Biol. Chem. 263, 708-13.

45. Sugar receptor mediated drug delivery to macrophages in the therapy of experimental visceral leishmaniasis. P.Chakroborty, A. Bhaduri and P.K. Das (1990) Biochem. Biophys. Res. Commun. 166, 404-410.

46. A high affinity Ca²⁺-ATPase on the surface membrane of Leishmania donovani promastigote. J. Ghosh, M. Ray, S. Sarkar and A. Bhaduri (1990) J. Biol. Chem. 265, 11345-51.

47. Neoglycoprotein as carriers for receptor-mediated drug targeting in the treatment of experimental visceral leishmaniasis. P.Chakroborty, A. Bhaduri and P.K. Das (1990) J. Protozool 37, 358-64.

48. Acivicin: A high active potential chemotherapeutic agent against visceral leishmaniasis. T. Mukherjee, K. Ray and A. Bhaduri (1990) Biochem. Biophys. Res. Commun. 170, 426-37.

49. Antileishmanial activity of hamycin: A polyene antibiotic. S. Sarkar, T. Mukherjee and A. Bhaduri (1992) Biochem. Biophys. Res. Commun. 182, 86-91.

50. Allosteric modulation of plasma membrane Ca²⁺-ATPase of Leishmania donovani. S. Majumdar, T. Mukherjee, J. Ghosh, M. Ray and A. Bhaduri (1992) J. Biol. Chem. 267, 18440-46.

51. Possible involvement of Ca²⁺in the in vitro morphogenetic transformation of L.donovani amastigote to promastigote. D. Sarkar, A. Ghosh and A. Bhaduri (1992) Proc. Natl. Acad. Sci. India B58(5), 1-7.

52. Mannose coated liposomal hamycin in the treatment of experimental leishmaniasis in hamsters. G. Banerjee, A. Bhaduri and M.K. Basu (1994) Biochem. Med. Metabol. Biol. 53(1), 1-7.

53. Temperature induced rapid increase in cytoplasmic free Ca²⁺ in pathogenic Leishmania donovani promastigotes. D. Sarkar and A. Bhaduri (1995) FEBS Letters 375, 83-86.

54. Characterization of the respiratory chain of Leishmania donovani promastigotes. K.R. Santhamma and A. Bhaduri (1995) Mol. Biol. Parasitol. 75, 43-53.

55. Ca2+-ATPase of Leishmania donovani is an extrusion pump for Ca²⁺. D. Mandal, T. Mukherjee, S. Sarkar and A. Bhaduri (1997) Biochem J. 322, 251-57.

56. Characterization of a calcium and calmodulin dependant protein phosphatase from Leishmania donovani. C. Banerjee, D. Sarkar and A. Bhaduri (1999) Parasitology 118, 567-73.

57.Leishmania plasma membrane Mg²⁺ -ATPase Is a H⁺/K⁺-antiporter involved in glucose symport. T. Mukherjee, A. Bhaduri et. al. (2001) J Biol Chem. 23; 276(8): 5563-9.
[C] Publications on Tea Research

58. Characterization of -galactosidase from Indian tea leaves (Camellia sinensis). J. Halder and A. Bhaduri (1997) The Journal of Nutritional Biochem. 8, 378-84.

59. Isolation and characterization of polyphenol oxidase from Indian Tea leaves (Camellia sinensis). J. Halder, P. Tamuli and A. Bhaduri (1989) The Journal of Nutritional Biochem.9, 75-80.

60. Protective role of black tea against oxidative damage of human red blood cells. J. Halder and A. Bhaduri (1998) Biochem. Biophys. Res. Commun. 244, 903-7.

61. Black tea is a powerful chemopreventor of reactive oxygen and nitrogen species: comparison with its individual catechin constituents and green tea. A. Sarkar and A. Bhaduri (2001) Biochem Biophys Res Commun. 284(1): 173-8.
[D] Other Publications/Books/Monographs

1. Current Trends in Leishmania Research (A. Bhaduri, M.K.Basu, A.K.Sen and S. Kumar, eds) (1995) Publication and Information Directorate, CSIR.