Electronic Posters: Cardiovascular

Tuesday 13:30-15:30 Computer 33

13:30 3636. Feasibility of 3D Late Enhancement Imaging in Mice with Totally Occluded Left Anterior Ascending (LAD) Artery on a Clinical 1.5T MR Scanner

Christian Kremser¹, Jakob Völkl², Bernhard Haubner², Michael Schocke¹, Bernhard Metzler<sup>2

1</sup>Dept. of Radiology, Innsbruck Medical University, Innsbruck, Tyrol, Austria; ²Dept. of Internal Medicine III (Cardiology), Innsbruck Medical University, Innsbruck, Tyrol, Austria

Delayed enhancement cardiac magnetic resonance imaging is frequently used to detect and quantify the size of myocardial infarction. In this study we demonstrate the feasibility of 3D late enhancement imaging in a mouse model on a clinical 1.5T whole body MR scanner and compare the obtained results with 2D sequences as used for clinical applications and histologic sections.

14:00 3637. Ex Vivo and in Vivo MR Imaging of Ischemia Reperfusion Injury in Mouse Hearts Using Microparticles of Iron Oxide Targeting VCAM-1

Erica Dall'Armellina¹, Craig A. Lygate¹, Martina McAteer¹, Steffen Bohl¹, Lee-Anne Stork¹, Stefan Neubauer¹, Robin P. Choudhury¹, Jurgen E. Schneider<sup>1

1</sup>Cardiovascular Medicine, University of Oxford, Oxford, Oxon, United Kingdom

Ischemia-reperfusion (IR) injury is an important cause of tissue damage in vascular syndromes of the heart, but sensitive markers of early inflammation in reversible myocardial injury are lacking. Our study demonstrates that antibody-conjugated microparticles of iron oxide (MPIO) targeting VCAM-1 enable molecular MR imaging of endothelial activation in murine IR hearts.

14:30 3638. A Fast Black Blood Sequence for 4D Cardiac MEMRI of Mouse Heart

William Lefrançois¹, Sylvain Miraux, Guillaume Calmettes, François Vigneron², Jean-Michel Franconi, Philippe Diolez, Eric Thiaudière

¹Résonance Magnétique des Systèmes Biologiques, UMR 5536 CNRS-Univ. Bordeaux 2, Bordeaux, Gironde, France, Metropolitan; ²INSERM U828, Avenue du Haut Lévêque, Bordeaux, France

This study aimed to develop a new method enabling a fast time-resolved cine 2D and cine 3D (4D) black blood imaging of mouse heart. This sequence has been applied to Manganese-Enhancement MRI (MEMRI) studies i.e. with Mn²⁺ infusion to improve contrast. This new method provided time- and space-resolved 3D images, respectively (200 µm) ³ and one image every 12 ms, for the first time within 30 minutes only. Lastly, associated to manganese infusion, this sequence appeared to be particularly adequate for studying cardiac pathologies such as ischemia on animal models.

15:00 3639. Black-Blood Preparation Improves Accuracy in Murine Phase-Contrast Cine MRI at Ultra-High Mag-Netic Fields

Erica Dall'Armellina¹, Bernd Jung², Hannah Barnes³, Stefan Neubauer¹, Michael Markl², Jurgen E. Schneider<sup>1

1</sup>Cardiovascular Medicine, University of Oxford, Oxford, Oxon, United Kingdom; ²Diagnostic Radiology, Medical Physics, University Hospital Freiburg, Freiburg, Germany; ³University of Oxford, Cardiovascular Medicine, Oxford, Oxon, United Kingdom

Tissue Phase Mapping (TPM) is a well-established technique to assess regional cardiac function in humans and in animal models such as mice. While TPM-studies in humans required suppression of the dominant blood signal in order to provide an accurate measurement of myocardial velocities, the murine studies were conducted without blood suppression. We show that bright-blood contrast can impact on both, absolute velocities and motion pattern, which can potentially and erroneously be identified as a local impairment of cardiac function.