First semester
Yüklə 299,6 Kb.
|
- Bu səhifədəki naviqasiya:
- Paper Code:BO4-554 : Practical: Plant Biotechnology and Plant Breeding
Hard Core 1: PracticalPaper Code:BO4-553 : Practical: Cytogenetics and Molecular Biology
1. Electron microscope, 2. PCR unit, 3. Gel documentation, 4. Isoelectric focusing, 5. Protein / amino acid analysis 6. DNA synthesizer 7. Column chromatography 8. ELISA 5. Enzyme as a function of its pH 6. Enzyme as a function of its concentration 7. Assay of lipase activity 8. Isolation of DNA 9. Estimation of protein 10. Estimation of ascorbic acid 11. Estimation of amylase activity 12. Secondary metabolite extraction and detection of antifungal secondary metabolites produced by plant tissues.
FOURTH SEMESTER Hard core-2 Plant Biotechnology and Plant Breeding Paper Code: BO4-552 80 Hours Objectives : To study how genetic engineering involves the use of recombinant DNA technology for crop improvement. To acquire fundamental knowledge on the application of biotechnology tools and techniques for improvement of microbes and higher plants. To understand the utility of medicinal plants and their conservation. Plant Biotechnology Unit 1 : Scope and importance of tissue culture techniques. Basic aspects of plant tissue culture, tissue culture media components, growth regulators, growth retardants, Vitamins, Amino acids, undefined supplements. Explants, sterilization, inoculation, sub-culturing. Different types of cultures, callus formation, different types of suspension cultures, single cell culture, testing of viability of cells, organogenesis-different types, factors affecting organogenesis. 12h Unit 2 : Somaclonal variation and its importance. Somatic embryogenesis - different methods, factors affecting embryo maturation, application of somatic embryogenesis, synthetic seeds - its significance. 04h Unit 3 : Protoplast isolation and culture, factors affecting. Protoplast fusion and somatic hybridization, fusion methods, fusion products, cybrids, applications of protoplast fusion. Haploids-different methods, anther and pollen culture, significance in crop improvement, ovary, ovule, endosperm and embryo culture, importance, in vitro fertilization and its significance. 08 h Unit 4 : Production of pathogen free plants, different methods, meristem culture and its importance in commercialization, development of disease - free tissue culture plants. Different types of secondary metabolites, production, factors, affecting yield, Biotransformation, different types with examples, immobilization techniques and advantages, biosensor and biochips, secondary metabolites production centers in India 12h Unit 5 : Germplasm storage- In vitro methods, long term and short-term cryo preservation and significance, cryo preservation centers in India. Micropropagation, different stages of micropropagation, rooting and establishments in herbs & woody plants. Restoration of degraded land through micropropagation, and development of stress tolerant plants 08h Unit 6 : Transgenics in crop improvement, plantibodies, oral vaccines, biodegradable plastics from plants 04h Unit 7 : Breeding methods with self pollinated crops (pure line breeding and mass selection, pedigree method, bulk population and back cross breeding, multiline, population breeding approach (diallel selective mating), rapid isolation of homozygous lines, Hybrid varieties - male sterilities). Breeding methods with cross pollinated crops. Selection in cross pollinated crops. Hybrid varieties. Recurrent selection. Synthetic composites varieties 08h Unit 8 : Genetic basis of breeding cross pollinated crops: Genetic basis of self incompatibility and male sterility and their use in hybrid seed production; genetic basis of inbreeding depression and heterosis; exploitation and fixation of heterosis; genetic basis of population improvement 08h Unit 9 : Polyploidy in plant breeding; Aneuploidy (nullisomics, monosomics, trisomics, monoploids and haploids), autopolyploidy, allopolyploidy, classical examples: evolution of bread wheat. Nicotiana tobacum, Gossipium hirsutum, amphideploids in Brassica species 08h Unit 10 : Mutation breeding: distant hybridization ,mutant variety data (MVD)-IAEA. Crop varieties: identification, release and notification of crop varieties, institutions involved in release of varieties. Quality seed, seed industry, seed act, seed production, processing, seed testing , IPR, PBR, breeders and farmers rights 08h Hard Core 2: Practical Paper Code:BO4-554 : Practical: Plant Biotechnology and Plant Breeding1. Instruments:a) Hot air oven, b) Autoclave, c) pH meter, d) Laminar air flow, e) Culture racks, f) Inverted microscope, g) Filtration unit and h) Accessories 2. Types of media and their constituents 3. Explants preparation – Monocot and Dicot 4. Media and explants sterilization 5. T/S of root and shoot primordia 6. Preparation of synthetic seeds 7. Isolation of protoplast and viability testing 8. Micropropagation – stem, leaf, meristem, flower bud, haploid culture (anther), embryo culture
Plant breeding experiments 10 Experimental calculations on Mendelian crosses, .cytoplasmic inheritance, and multiple alleles 7 Floral biology in self and cross pollinating plant species, selfing, crossing 8 ANOVA, means, variances, partitioning of variances in various models 9 Estimation of genetic diversity through D2 statistic, correlation, heritability, and 10 Seed health testing for germination 11. Creation and screening plants for biotic and a biotic stress References
Yüklə 299,6 Kb. Dostları ilə paylaş:
|