Supplemental text


Nicotinamide-nucleotide adenylyltransferase



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Nicotinamide-nucleotide adenylyltransferase

no

no

no

Table S10. LOS biosynthesis genes

Genes identified in H. influenzae 86-028NP (18)


Gene

H. influenzae

86-028NP



H. influenzae Rd

H. somnus 129Pt

H. ducreyi 35000HP

kdsB

3-deoxy-D-manno-octulosonate cytidylyltransferase



NTHI0068

HI0058

HS_0658

HD0334

lpxK

tetraacyldisaccharide-1-P 4'-kinase



NTHI0069

HI0059

HS_0656

HD0217

msbA

Lipid A ABC transporter, ATP-binding protein



NTHI0072

HI0060

HS_1021

HD1630

msbB

lipid A biosynthesis (kdo)2-(lauroyl)-lipid IVA acyltransferase



NTHI0296

HI0199

HS_1202

HD0404

lgtC

glycosyl transferase


repeats?

NTHI0365

GACA repeated 10 times in frame



HI0258

GACA repeated 22 times in frame just after start codon



HS_0636 lob2D

CAGT repeated 19 times in the middle of the CDS



HD1090

no repeats



orfM

Xanthosine triphosphate pyrophosphatase



NTHI0366

HI0260

HS_0125

HD1348

kdkA

3-deoxy-D-manno-octulosonic acid kinase,



NTHI0367

HI0260.1

no

HD1101

opsX/rfaC

ADP-heptose:LPS heptosyltransferase I




NTHI0368

HI0261

HS_1611

lipopolysaccharide heptosyltransferase-1



HD0445

lpt6

phosphorylethanolamine transferase



NTHI0383

HI0275

HS_0234

no

galE

UDP-glucose 4-epimerase



NTHI0471

HI0351

HS_0789

HD0829

lic3A

alpha-2,3-sialyltransferase




NTHI0472
CAAT repeated 18 times in frame

HI0352


no

no

possible acetyltransferase

NTHI0512
AGCA repeated 8 times in frame

HI0391
no repeats

HS_0553
no repeats

no

waaQ

ADP-heptose:LPS heptosyltransferase III



NTHI0649

HI0523


no

HD1202


lic2A

UDP galactose lipooligosaccharide galactosyltransferase



NTHI0677

CAAT repeated 14 times in frame



HI0550

CAAT repeated 21 times in middle of CDS



HS_0637 lob2A

TGAT repeated 36 times in 300 bases upstream of CDS start



Top hit is to lgtB HD0472

no repeats



kdtA

3-deoxy-d-manno-octulosonic-acid transferase



NTHI0772

HI0652

HS_1590

HD0454

lgtF

UDP-glucose--Lipooligosaccharide glucosyltransferase




NTHI0773

HI0653

HS_0291

HD1201

lpxH

UDP-2,3 diacylglucosamine hydrolase




NTHI0892

HI0735

HS_0498

HD1938

manB phosphomannomutase

NTHI0899

HI0740

HS_1670

HD1507

lex2B

UDP-glucose--lipooligosaccharide glucosyltransferase



NTHI0913

this is a family 25 glycosyltransferase



top hit is HI1697

a family 25 glycosyltransferase




HS_0638 lob1 glycosyltransferase, family 25

top hit is HD1721

a family 25 glycosyltransferase



lic2B

glycosyltransferase lpsA homolog



NTHI0926

HI0765

HS_0637 lob2A is the top hit

HD0472 lgtB is the top hit

galU

glucosephosphate uridylyltransferase



NTHI0976

HI0812

HS_1117

HD1431

lic3A2

CMP-neu5Ac--lipooligosaccharide alpha 2-3 sialyltransferase




NTHI1034
CAAT repeated 18 times in frame

HI0352
32 CAAT repeats and an earlier start are 317 bases upstream of the gene start. The earlier start and repeats are out of frame with the NTHI1034 gene.

no

no

rmlB/rffG

DTDP-glucose 4,6-dehydratase



NTHI1037

HI0873

HS_0707

HD0687

lpxD

UDP-3-O-(3-hydroxymyristoyl)-glucosamine N-acyltransferase



NTHI1082

HI0915

HS_0978

HD1189

predicted PE--lipooligosaccharide phosphorylethanolamine transferase

NTHI1180

HI1005

no

HD0371

lpxB

lipid-A-disaccharide synthetase



NTHI1220

HI1060

HS_1358

HD0846

lpxA

UDP-N-acetylglucosamine acetyltransferase



NTHI1222

HI1061

HS_1359

HD1187

predicted PE-lipooligosaccharide phosphorylethanolamine transferase

NTHI1224

HI1064

no

HD1598

rfaF

ADP-heptose--LPS heptosyltransferase II



NTHI1272

HI1105

HS_1612

HD0653

rfaD

ADP-L-glycero-D-mannoheptose-6-epimerase



NTHI1278

HI1114

HS_1613

HD1890

lpxC

UDP-3-0-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase



NTHI1312

HI1144

HS_0364

HD0816

lpcA/gmhA phosphoheptose isomerase

NTHI1350

HI1181

HS_1238

HD1228

lgtA

N-acetylglucosamine glycosyltransferase



NTHI1474

HI1578

no

HD0466

kdsA

2-dehydro-3-deoxyphosphooctonate aldolase



NTHI1576

HI1557

HS_0946

HD0857

licD

lipopolysaccharide choline phosphotransferase



NTHI1594

HI1540

HS_1458

HS_0727 fragment



no

licC

CTP:phosphocholine cytidylyltransferase



NTHI1595

HI1539

HS_1459

no

licB

choline transporter



NTHI1596

HI1538

HS_1460

no

licA

phosphorylcholine kinase



NTHI1597
CAAT repeated 15 times in frame

HI1537
alternative start and CAAT repeated 17 times out of frame prior to the gene start

HS_1461
no repeats

C-terminal only HD1021

no repeats



htrB

lipid A biosynthesis lauroyl acyltransferase



NTHI1606

HI1527

HS_0575

HD1106

rfaE

ADP-heptose synthase



NTHI1607

HI1526

HS_0576

HD1182

mrsA Phosphomannomutase

NTHI1664

HI1337

HS_0730

HD0201

glycosyltransferase

NTHI1750
GACA repeated 14 times in frame

HI0258 is top hit

HS_0636 lob2D is top hit ~50% identity

HD1090?
no repeats

glycosyltransferase

NTHI1769
CCAA repeated 17 times out of frame

HI1386
CCAA repeated 16 times in 300 bases upstream of gene start

no

HD0375 is top hit

no repeats




neuA

acylneuraminate cytidylyltransferase



NTHI1891

HI1279

HS_0706

HD0685

Possible polysaccharide biosynthesis protein

NTHI1921

HI1244

no

HD1508

lsgF

lipopolysaccharide biosynthesis protein



NTHI2002

HI1695

no

HD0886

lsgE

lipopolysaccharide biosynthesis protein



NTHI2003

HI1696

no

HD0885

lsgD

lipopolysaccharide biosynthesis protein



NTHI2004

HI1697

no

HD0883

lsgC

lipopolysaccharide biosynthesis protein, putative




NTHI2005

HI1698

no


no

lsgB

lipopolysaccharide biosynthesis protein

beta-galactoside alpha-2,3-sialyltransferase


NTHI2006

HI1699

no

no

lsgA

lipopolysaccharide biosynthesis protein



NTHI2007

HI1700

HI0867


no

HD0882

rfe/mraY/wecA undecaprenyl-phosphate alpha-N-acetylglucosaminyltransferase

NTHI2025

HI1716

HS_0355

HD1844

B. Genes identified in H. influenzae Rd (20) that are not included above



Gene


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