Analysıs of Endogenous Sırevırus ın Barley (Hordeum Vulgare L.)
In this study, it was investigated activation of SIRE1 retrotransposon on barley by using IRAP technique. It was used 4 root and 4 leaf DNA belong to same seed. We used mature barley embryos as control material. It was observed polymorphic band patterns for embryo, root and leaf DNA at the end of IRAP analysis. It was found that polymorphism rates among embryo, root and leaf between 0-64 %. Polymorphism rates was determined among embryos 0-27 %, among roots 8-60 %, among leaves 11-50 %. Polymorphism which was seen among different roots and leaves, was also seen roots and leaves belong to same seed (% 11-64).
In addition to IRAP analysis, it was analyzed gag, rt and env domains in embryos, roots and leaves. It was detected these domains in embryos, roots and leaves. It was observed different band patterns in samples at the end of rt and env analysis. This variation results from developmental period, sequence duplication or sequence deletion. SIRE1 gag, rt and env domains was sequenced. As a result of BLAST analysis, it was found that similarities of SIRE1 retrotransposon to Ty1- Copia retrotransposons were 79 % for gag domain, 84 % for rt domain and 95 % for env domain.
