Tez özetleri Astronomi ve Uzay Bilimleri Anabilim Dalı

The Effects of Vitamin C on Sodium Fluoride-Induced Oxidative

Damage on TM4 Sertoli Cells
In this study, fluoride-induced oxidative damage and the protective effect of Vitamin C which is a strong antioxidant supplementation against this damage on TM4 Sertoli cells were purposed to research by determination of cell viability, cell proliferation, cytotoxicity, amount of glutathion, antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase), steroidogenic enzymes (3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase), lipid peroxidation, hydroxyl radical, hydrogen peroxide, apoptosis/necrosis rate and γ-glutamyltranspeptidase enzyme activity which is a marker for Sertoli celll function.
Fluorine is found widely in drinking water and foods threat to the environment and human health. Organic and inorganic fluorine is found in all sources of soil and water and is taken by human with plant and animal consumption. Toxicity of fluorine in fluoride is concerned with water solubility. Sodium fluoride which is easily soluble in water is more toxic than calcium fluoride, rock phosphate or cryolite. The most important and indispensable ways of sodium fluoride to take the human body are drinking water, food, drugs, tooth paste and dental products.
It is known that fluoride damage to the cell generating free radicals. Sufficient amount of antioxidant must consume with diet for prevention of oxidative stress that occurs free radicals and decreasing of its effect. Vitamin C is a strong antioxidant for its powerful reductive activity. Vitamin C reacts with superoxide radical and hydroxyl radical, thus scavenges them from environment. Therefore, vitamin C protects the cell from oxidative damage.
Sodium fluoride was exposed to Sertoli cells for 24 and 48 hours at 4 ppm and 20 ppm doses and, in addition to these doses, to vitamin C (50 μM). Cell viability, cell proliferation, amount of glutathion, antioxidant enzymes, steroidogenic enzymes, lipid peroxidation, hydroxyl radical, hydrogen peroxide, cytotoxicity, apoptosis/necrosis rate were measured in Sertoli cells. The results indicated that cell viability, cell proliferation, steroidogenic and antioxidant enzymes are decreased, while lipid peroxidation, hydroxyl radical, hydrogen peroxide, cytotoxicity, apoptosis/necrosis rate are increased. This findings is indicate that sodium fluoride induced apoptosis depending on generation of reactive oxygene species (ROS) and lipid peroxidation and inhibited testosterone biosenthesis by inhibiting steroidogenic enzymes. Furthermore, it was revealed that vitamin C has a protective effect on toxicity caused by sodium fluoride.