Tez özetleri Astronomi ve Uzay Bilimleri Anabilim Dalı

The Effects of Vitamin C, Vitamin E, -Carotene and Selenium on Indomethacin-Induced Gastric Mucosal Damage

Non-steroidal anti-inflammatory drugs (NSAID) are widely used in the treatment of pain, fever and inflammation. These drugs have some side effects especially on the gastrointestinal tract such as gastric mucosal erosions, ulcerations, bleeding and perforations. The gastrointestinal mucosal lesions are experimented by stress, indomethacin, reserpine, ethanol, acids, bases or ischemic-reperfusion. Reactive oxygen species (ROS) play a vital role in the mucosal lesion associated with NSAID induced gastrointestinal damage. ROS damaged numerous biochemical structures particularly lipids, proteins and nucleic acids. Antioxidant defense systems and their components, including antioxidant enzymes, foods and drugs are important in preventing the toxic and disease causing effects of oxygen-derived free radicals. The purpose of the present study was to investigate the gastroprotective effects of combination of vitamin C, vitamin E, -carotene and selenium against indomethacin-induced gastric injury in rats. In this study, male Sprague-Dawley rats were used. The rats were randomly distributed into 6 groups. Group I; intact animals. Group II; control animals receiving vit.C (100 mg/kg), vit.E (100 mg/kg), -carotene (15 mg/kg) and sodium selenate (0.2 mg/kg) for 3 days, orally, daily. Group III, Rats receiving 25 mg/kg indomethacin. Group IV; animals receiving vit.C , vit.E , -carotene and sodium selenate for 3 days (in the same dose and time) , 2 h prior to the administration of indomethacin. Group V; control animals receiving 150 mg/kg ranitidine. Group VI; animals receiving ranitidine for 3 days, 2 h prior to the administration of indomethacin. At 6 h after the indomethacin administration all the animals were sacrificed expose to ether. The administration of indomethacin caused a decrease in the levels of blood glutathione, tissue mucin and hexosamine content and glutathione-S-transferase acitivity and an increase in the serum aspartat amino transferase, alanin amino transferase and alkaline phosphatase activities, tissue lipid peroxidation and protein carbonil content, lactate dehydrogenase, catalase activities. Treatment with vitamin C, vitamin E, -carotene, selenium and ranitidine revised these effects. These results suggested that the gastroprotective effect of vitamin C, vitamin E, -carotene and Se can be attributed to its reducing effect on the oxidative damage.

KARA Beyhan
Danışman :Prof. Dr. Refiye YANARDAĞ

Anabilim Dalı :Kimya

Programı :Biyokimya

Mezuniyet Yılı :2009

Tez Savunma Jürisi : Prof.Dr. Refiye YANARDAĞ

Prof.Dr. Nuriye AKEV

Prof.Dr. Ayşen YARAT

Prof.Dr. İnci ARISAN-ATAÇ

Yrd.Doç.Dr. Özlem SAÇAN