Traditional Posters: Miscellaneous
Microscopy Hall B Monday 14:00-16:00
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| Microscopy
Hall B Monday 14:00-16:00 1037. Characterization of Tumor Microvascular Structure and Permeability by MRI and Intravital Confocal Imaging Nina Kristine Reitan1, Marte Thuen2, Pål Erik Goa3 1Department of Physics, NTNU, Trondheim, Norway; 2Department of Circulation and Medical Imaging, NTNU, Trondheim, Norway; 3Department of Radiology, St. Olavs Hospital, Trondheim, Norway By using confocal laser scanning microscopy (CLSM) and MRI we studied microvascular architecture and permeability in tumors growing in dorsal window chambers in mice. 40 kDa dextran and Gadomer was used as molecular tracers for dynamic CLSM and DCE-MRI, respectively. Correlation was found between permeability measured by the two techniques and permeability further depended on structural parameters, like fractal dimension and vascular density. This study demonstrates that the dorsal window tumor model gives an opportunity to use CLSM and MRI as supplementary techniques and that CLSM provides insight into the spatial heterogeneous microenvironment on a microscopic level that is not accessible with MRI. 1038. In Vivo High-Resolution Magic Angle Spinning Proton MR Spectroscopy of Small Whole-Model Organism C. Elegans Valeria Righi1,2, Alex A. Soukas3, Gary Ruvkun3, A Aria Tzika1,2 1NMR Surgical Laboratory, Department of Surgery, Massachusetts General Hospital and Shriners Burns Institute, Harvard Medical School, Boston, MA, United States; 2Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Athinoula A. Martinos Center for Biomedical Imaging, Boston, MA, United States; 3Department of Genetics, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States We demonstrate metabolic biomarker profiles with high-resolution magic angle spinning proton MR spectroscopy (HRMAS H1 MRS) of living Caenorhabditis elegans (C. elegans) worms. This work opens up perspectives for the use of H1 HRMAS-MRS as a metabolic profiling method for C. elegans. Because it is amenable to high throughput and is shown to be highly informative, this approach may lead to a functional and integrated metabolomic analytic approach of the small organism C. elegans, which has been used extensively in studies of aberrant metabolism, and should help in identifying, investigating, and even validating new pharmaceutical targets for metabolic diseases. 1039. Morphologic Abnormalities of Mucopolysaccharidosis Type VII Characterized by High Resolution MRI in a Mouse Model Ilya Michael Nasrallah1, Sungheon Kim2, Ranjit Ittyerah1, Stephen Pickup1, John H. Wolfe3,4, Harish Poptani1 1Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States; 2New York University; 3Departments of Pathobiology and Pediatrics, University of Pennsylvania; 4Children's Hospital of Philadelphia Mucopolysaccharidosis Type VII (MPS VII) is one of the degenerative lysosomal storage diseases characterized by intracellular vacuolization. Using high-resolution MRI in a mouse model of MPS VII with manual segmentation, we identify decreases in corpus callosum and anterior commisure volume and slight increase in hippocampus volume in mutant mice. A decrease in corpus callosum volume thickness is confirmed at histology. These parameters could be used for monitoring experimental response to gene therapy treatments. 1040. MRI Phenotyping of Craniofacial Development in Transgenic Mice Embryos Yüklə 355,76 Kb. Dostları ilə paylaş:
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