Executive Summary



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scientific

Lab 305

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences

86-10-64889783

100101

Beijing

Datun Road,Chaoyang District

China

lipid, gene cloning, gene function characterization

I am interested in improvement of oilcrops, lipid synthesis related gene cloning and gene function characterization.

scientific

Biochemistry and Nutrition Group, Biocentrum-DTU

The Technical University of Denmark

+45 45252744

2800

Lyngby

Building 224, DTU

Denmark

LC/MS, animal experimental facilities, GC, TLC

Research areas/competencies related with lipidomics: Identification and quantification of triacylglycerols and phospholipids by LC/MS, separation and quantification of different phospholipids and lipid classes by NP-HPLC, separation and quantification of triacylglycerol molecular species by RP-HPLC, separation and purification of lipids by TLC and SPE, analysis of fatty acid profiles by GC, analysis of lipid regiostructures by partial degradation (Grignard degradation for triacylglycerols & phospholipase degradation for phospholipids) followed with GC, GC/MS, LC/MS, analysis of plasma lipids by Cobas Mira, quantification of cholesterol in different lipoproteins using HPLC. Other research activities: Lipid absorption and metabolism, application of stable isotope techniques and GC/C/IRMS in metabolic studies, effect of dietary fats on learning ability and visual function, bioavailability of n-3 fatty acids, applying enzymatic technology in studies on lipid modification including structured triacylglycerols and structured phospholipids, studies on new dietary lipids such as diacylglycerols and conjugated linoleic acids, interactions of lipids with other food components, lipoprotein oxidation, development of analytical methods, especially emphasized on separation techniques and mass spectrometry for analysis of lipids and lipid soluble vitamins, qualitative and quantitative analysis of carbohydrates. We possess extensive expertise in lipid biochemistry and lipid analysis, and we have the following facilities: Analytical instruments for lipid analysis of food and biological systems (GC, HPLC, TLC, GC/MS, LC/MS, GC/C/IRMS, Cobas Mira, Spectrophotometer) Animal experimental facilities

scientific

University of Helsinki

Department of Medicine

+358 50 563 6899

00020

Helsinki

PO Box 700

Finland

liver insulin marker

Profiling of human serum in individuals with a fatty vs no-fatty liver in collaboration woth Matej Oresic.

scientific

Helsinki Biophysics & Biomembrane Group

Institute of Biomedicine

+358-9-19125400

FIN-00014 University of Helsinki

Helsinki

POB 63

Finland

lipids, drugs, proteins, amyloids

lipid phase behavior, bioactive lipids, lipid-protein interactions, drug-lipid interactions, cationic lipids in gene delivery, lateral organization of biomembranes

scientific

Institute of Biomedicine/Käkelä Group

University of Helsinki

++358-9-19125409

P.O. Box 63

Helsinki

Haartmaninkatu 8/P.O. Box 63

Finland

Lipid-protein interactions, Mass spectrometry, Ion channels, Fatty acids

-Interactions of lipid membrane and membrane proteins/ion channels -Cellular metabolism of lipids and fatty acids -Mass spectrometry of lipids

scientific

Institute of Biomedicine/Somerharju group

University of Helsinki

358-9-19125410

00014

Helsinki

Haartmaninkatu 8/PL 63

Finland

mass-spectrometry, trafficking, membrane,phospholipase

1)Lipid trafficking 2)Regulation of lipid composition of cells (lipid homeostasis) 3)Mode of action of phospholipases 4)Membrane structure, expecially lateral distribution of lipids 5)Atherosklerosis and other lipid related diseases 6)Development of MS tools for lipid analysis

scientific

Department of Biochemisty

University of Oulu

+358-8-553 1150

FIN-90014

Oulu

POB 3000

Finland

mitochondria, peroxisomes, structural enzymology

Lipids and metabolic disorders, yeast lipids, fatty acid synthesis, membrane lipidomics, lipid metabolism, lipid metabolizing enzymes,

scientific

Quantitative Biology and Bioinformatics

VTT Technical Research Centre of Finland

++358-20-720-4491

FIN-02044 VTT

Espoo

Tietotie 2, P.O. Box 1500

Finland

systems biology, early markers, metabolomics, phenotype characterization

Animal model phenotype characterization using lipid (and general metabolite) profiling approaches - Bioinformatics method for integration of lipid profile information from mass spectrometry based approches with other levels of data such as gene expression and protein profiles - Clinical applications of lipidomics for early disease detection and disease progression studies, focus on diabetes

scientific

Biological Chemistry Laboratory

Biological Chemistry LaboratoryNational Institute for Agronomic Research (INRA)

++33-1-30815474

78850

Thiverval Grignon

BP 1

France

oil, oil bodies, A thaliana, Y lipolytica, B napus

We are interested in understanding the biology of oil bodies, with special emphasis on assembly and degradation. We are currently studying two organisms: - A. thaliana, a model for for oil crop. -- Y. lipolytica, yeast capable to use and transform lipids. We combine a multiscale approach going from: -the identification of oil bodies proteins (proteomics, enzymatic studies, immunochemistry) --the study of isolated proteins (purified of recombined) in solution (enzymology, interfacial properties using hanging drop methods), or within model interfaces (Langmuir balance experiment) --- the study of proteins within complete organelle (from wt or mutants organisms) ----the study of wt and mutant model organism. ----- transcriptomic analysis of yeasts genes expressed or repressed upon shift from glucose to oleic acid medium. -Our goals are - to identify all protein component involved in oil bodies biogenesis -- to understand their role and identify their activity ---to study the influence of oil body protein composition on lipid content (nature, amount) Principal collaborations: -Within France Dr. M Miquel, Dr. L Lepiniec, Seed Biology Laboratory, INRA, Versailles: Molecular biology of A. thaliana seeds, Dr JM Nicaud Molecular Genetic Laboratory, INRA CNRS, Grignon: Molecular biology of Y. lipolytica. Dr. M Axelos: BIA Laboratory, INRA, Nantes. Interfacial methods -Within European Union Prof. G Daum, Dr K Athensdaedt, technical University, Graz: Lipid particles from yeast Dr. S Papanikolaou, Agronomic University of Athens, Biotechnology of Oleaginous yeast

scientific

INSERM-538 (biomembranes)

CHU Saint-Antoine

33-01-40011340

75571

PARIS 12°

27 rue Chaligny

France

membrane, microdomains, traffic, phospholipids

Membrane heterogeneity and polarized cellular traffic. Cholesterol and sphingolipids enriched membrane microdomains Rotavirus traffic in Caco-2 cells. Synthesis of bile phospholipids

scientific

Laboratory of Enzymology at Interfaces and Physiology of Lipolysis

CNRS

33 4 91 16 41 34

13402

Marseille cedex 20

31 chemin Joseph Aiguier

France

lipases, TLC-FID, monolayers

Lipolytic enzymes and their use for the characterization lipids Production facilities, purification and kinetic characterization of several recombinan t lipases, including gastric and pancreatic lipases, pancreatic lipase-related proteins, hormone-sensitive lipase, microbial lipases, Plant phospholipase D, phospholipase A2, phospholipase A1, galactolipase Use of thin-layer chromatography coupled to flame ionization detection (TLC-FID, Iatroscan) for quantitative measurement of neutral and polar lipid species and their lipolysis products. Use of the monomolecular film technique for studying lipid-protein intercations and lipolytic enzymes kinetics. This technique allows measurement of lipolytic activity with very low amounts of substrate (g)and is therefore suitable for rare lipids.

scientific

CIML

CNRS INSERM

33 491269404

13288

marseille

parc sxcientific de luminy

France

abc transporters, abca1, malaria, flippase

lipid transport /flip by abac transporters of the A class lipidomic analysis of microparticles during malaria infection

scientific

Laboratoire de Biogenèse Membranaire

CNRS UMR 5200 Université V. Segalen Bordeaux 2

+33 (0)5 57 57 10 45

33076

Bordeaux cedex

146 Rue Léo Saignat

France

Plant lipids, waxes, fatty acids, GC, Lipid analysis

The Membrane Biogenesis Laboratory (MBL) has a long history of research in membrane biogenesis and lipid biosynthesis. This lab is internationally known and has been recently chosen to organise the International Plant Lipid Symposium in 2008. The aims of the research concern the identification and the characterisation of the genes involved in the regulation of lipid metabolism. Using Arabidopsis thaliana as the main plant model, the topics currently developed in the unit are: i/ research project I: Genomics of epicuticular wax metabolism. The objective is to characterise the genes involved in the biosynthesis of epicuticular waxes in order to understand the regulatory mechanisms of the expression of genes, which control the composition of the wax layer in response to various stress conditions. ii/ research project II: Membrane biogenesis and homeostasis. This project aims to characterise the mechanisms controlling the membrane lipid composition, the lipid transfer between organelles and membranes, and the lipid composition of lipid rafts in relation to hormonal signalling. iii/ research project III: SNAREs, lipids and endomembrane dynamics. The objective is to study the role of SNAREs in membrane dynamics between the ER and the Golgi apparatus in the secretory pathway leading to the biogenesis of plasmalemma and the formation of lipid rafts through the secretory pathway. The MBL is one unit of the IFR 103 Integrative Biology which involves 6 labs covering the different areas of plant research (pathogenesis, biotechnology, agronomy), and technological platforms (imaging, lipidomic, metabolism, transcriptome). The MBL has in charge the lipidomic platform which consist of 3 GLC,1 GC/-MS 1 densitometer, 1 phosphor imager and 2 TLC spotters. This platform allows lipid and fatty acid analysis and lipid metabolism studies.

scientific

Institute of molecular and cellular pharmacology

CNRS UMR6097

++33-(0)4-93957733

06560

Valbonne

Sophia Antipolis- 660 route des lucioles

France

phospholipase A2, lipid mediator, cancer, inflammation

Eleven genes coding for secreted phospholipases A2 (sPLA2s, 14-18 kDa) and 2 types of receptors (M and N) have now been identified in mammalian tissues. sPLA2s and their receptors are found in several tissues, and their expression levels are increased in inflammation, associated diseases, different types of cancer, and neurodegenerative diseases. Although the biological functions of sPLA2s and their receptors are still ill-defined, some sPLA2s are mitogenic and/or apoptotic, pro-inflammatory and/or pro-tumoral, but also anti-tumoral, anti-bacterial, anti-viral, and anti-parasitic. At the molecular level, sPLA2s are most likely bifunctional proteins, acting as both enzymes and ligands for a variety of soluble and membrane proteins. sPLA2 enzymatic activity participates in the control of the rate-limiting step in the production of lipid mediators such as prostaglandins and leukotrienes which are involved in a myriad of biological effects. We have previously identified for the first time the M and N type receptors for sPLA2s using snake venom sPLA2s as ligands. More recently, we have cloned 7 of the 11 known mammalian sPLA2s, and we have shown that several of these enzymes are the natural ligands of the M-type receptor, suggesting that mammalian sPLA2s are true ligands for mammalian proteins. The main objective of our research program is to determine the function of sPLA2s, while continuing their molecular characterization. Our specific aims are : 1) To analyze the molecular properties of sPLA2s and to develop key tools to determine sPLA2 function (recombinant production, native structure, crystallization, enzymatic and binding properties, search of specific inhibitors, characterization of receptors, transgenic mice), 2) To analyze the tissue distribution of sPLA2s and their receptors in normal and pathological states in relation with the following item, 3) To study the role of sPLA2s in three major research areas : colorectal cancer, inflammation, and host defense against viruses, bacteria, and parasites.

scientific

INSERM U467/IFR94 Proteomics Platform

Faculté de Médecine René Descartes Paris 5

+33 1 40 61 56 21

75015

Paris

156 rue de Vaugirard

France

lipid rafts, eicosanoids, epithelium

Protein-lipid interactions, proteomics and lipidomics of lipid microdomains, and their relevance in physiopathology of epithelial cells. Regulation of inflammation in cystic fibrosis.

scientific

French Institute for Fats and Oils (ITERG)

Industrial Technical Center

+33 5 56 36 00 44

33600

PESSAC

rue Monge

France

nutrition bioavailability cancer CHD

Nutritional impact of lipids (in relation to diseases : CHD, cancer, obesity). Biomarkers studies (adipose tissue, plasma, erythrocytes).

industrial

IMBL-Lipid Signaling (UMR 585 INSERM / INSA-Lyon)

INSA-Lyon and CNRS / INSERM / Univ Lyon1

33-4-72 43 82 40

69621

Villeurbanne

20 Ave A. Einstein

France

Signaling , Fatty acids , Lipid mediators , Analysis

INSERM UMR 585 / INSA-Lyon is part of IMBL (Institute for Multidisciplinary Biochemistry of Lipids). This UMR works on lipid signaling, in blood, vascular cells and adipocytes, in the context of aging, atherosclerosis, diabetes and obesity. Lipids of interest are membrane phospholipids as reservoirs of polyunsaturated fatty acids which serve as precursors of eicosanoids and docosanoids. The production of those oxygenated metabolites through cyclooxygenase and lipoxygenase pathways is the main focus. The role of oxidative stress/lipid peroxidation in those pathways as well as their control by polyunsaturated fatty acids of nutritional value are of special interest. The sphingomyelin / ceramide / sphingosine / sphingosine-1-phosphate is also taken into consideration.

scientific

INSERM U671

INSERM

++33142346923

75006

Paris

15 rue de l ecole de medecine

France

adipocyte obesity lipid droplet

Synopsis of current research interest in the field of lipid droplet biology: We are working in the field of metabolic diseases associated with obesity in an INSERM unit in Paris (UMR 671). In these diseases, ectopic lipid storage is a key deleterious event at the whole body level, associated with grave metabolic complications. In this regard, we are interested in lipid trafficking in adipose cells specialized in the storage of fat. Caveolins have been found at the surface of lipid droplets, but their functional role in the dynamics of fat storage remains unknown. Since caveolins are highly expressed in adipocytes, and caveolin-KO mice have revealed a striking inability to store fatty acids from extracellular sources into their adipose tissue, we focused our research on the role of caveolins in lipid homeostasis. In a recently published paper (Le Lay et al., Traffic 2006), we have shown that adipocyte lipid droplet composition was dependent on caveolin expression and that exogenous cholesterol was able to induce caveolin targetting to these lipid droplets through a process sharing many features in common with caveolar endocytosis. We are now interesting in elucidating the dynamic relationship between cell surface and lipid droplets through caveolin trafficking, and identifying the adipocyte lipid species that can be stored through a caveolin dependent pathway. In this line, our future projects deal with the functional role of caveolins in lipid targeting to the fat storage compartment, the lipid droplet.

scientific

INSERM U586 (Obesity Research Unit)

INSERM,

33-5-62172956

31432

Toulouse

CHU Rangueil BP84225

France

lysophosphatidic acid, autotaxin, lysophospholipase D, adipocyte,

My group is interested in the regulations and the biological consequences of the release of lysophosphatidic acid (LPA) by adipocytes. We are particularly interrested in a lysophospholipase D (autotaxin) which appears to be the main source of LPA release by adipocytes but which could also be involved in synthesis of several other bioactive phospholipids. ATX expressio is adipose tissue is up-regulated in association with insulin-resistance and type 2 diabetes. In paralelle, we are also interrested in understanding the transduction pathways (particularly the receptors) involved in LPA actions on the different cell types surrounding adipocytes in the adipose tissue including preadipocytes, macrophages, endothelial cells.

scientific

IFR30 Lipid Analysis Platform

INSERM/Toulouse Genopole

33 (0)561779414

31024

Toulouse

CPTP Bat C, Hopital Purpan BP3028

France

neutral lipid, ceramide, bile acid, phytosterol

Our Lipid Analysis Platform belongs to the French Nationale Institute for Health and Medical Research (INSERM). It is located in the Federative Research Institute (IFR30) in Toulouse, France and is associated to the Toulouse Genopole. The platform created in 2003 proposes a number of lipid analysis using chromatographic methods, either adapted from the litterature or original. We are equiped with 3 gas chromatographs (GC) with FID detection, one GC coupled to a mass spectrometer and one liquid chromatograph (HPLC) coupled to a light scattering detector (LSD). We propose qualitative and quantitative analysis of lipid molecules following extraction from micro-samples (biological fluids, tissues, cells). We thus have access to the molecular species of the following lipids : - Neutral lipids including cholesterol and related sterols, diacylglycerol, cholesterol ester and triacylglycerol (on the basis of carbon number) - Free or total fatty acids - Sphingomyelines and usual ceramides - Phytosterols - Bilary acids (conjugated or free) - We are in progress to propose in the near future the analysis of major phospholipids classes, galactolipids and cerebrosides by HPLC-LSD. Analysis are proposed on the basis of a price per sample. Collaboration on specific projects is opened. Present ongoing projects include the fields of intestinal lipid absorption, cancer and apoptosis, lipoprotein metabolism, nervous system functionnning, lipid transduction


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