2011 YÜksek lisans tez özetleri

Production Of Cellulose-Silver Nanoparticles And Determination Of Their Biological Activity As Disinfectant

The antibacterial properties of a novel cellulose- Ag-nanoparticle composite is investigated in this study. According to the previous studies, cellulose alone has not antibacterial activity, although cellulose-Ag nanoparticle composite has good antibacterial activity. Experiments indicated that cellulose and cellulose acetate have no antibacterial properties. However, they have antibacterial properties when they became composite with silver nanoparticles. Antibacterial effect of cellulose acetate increased by increasing AgNO₃ concentration. But cellulose’s effect is mostly for 0,2 M AgNO₃ concentration.

Cellulose-silver nanoparticle composite synthesized by two different methods, NaBH₄ as a reducer in AgNO₃ solution and at 95°C in NaOH solution in AgNO₃ solution. In this study, cellulose acetate-silver nanoparticle composite is synthesized by stirring mixture of AgNO₃ and 2-methoxyethanol during 2 hours at 50°C. Silver nitrate incorporated into cellulose acetate (CA) polymer as a dispersion medium and these silver-containing polymer films are investigated to understand the interactions between silver complexes and cellulose acetate molecules, the formation of Ag nanoparticles with their size control, and their reaction chemistry in cellulose acetate polymer. X-ray Fluorescence (XRF) patterns indicated the existence of silver nanoparticles in the cellulose, structure changes investigated by FTIR and silver concentration investigated by UV in cellulose acetate and scanning electron microscopy (SEM) images showed that the silver nanoparticles well dispersed on the surface of cellulose and penetrated into the cellulose network.

Escherichia coli (ATCC 25922), Acinetobacter baumannii (ATCC19606), Staphylococcus aureus (ATCC25923), Enterococcus faecalis (ATCC29212), Pseudomonas aeruginosa (ATCC27853), Streptococcus pneumoniae (ATCC49619) is used to test the bactericidal efficacy of cellulose and cellulose acetate–Ag-nanoparticle composite. The biological activity is determined by the minimum inhibitory concentration (MIC) of the composite.