Bibliographic Information Self-gelatinizable copolymer immobilized glucose biosensor based on prussian blue modified graphite electrode

Bibliographic Information
Study on charge transport in polypyrrole film doped with electroactive ions. Li, Yijun; Yang, Chunhe; Dong, Shaojun. Changchun Institute Applied Chemistry, Chinese Academy Sciences, Changchun, Peop. Rep. China. Fenxi Huaxue (1997), 25(1), 1-4. CODEN: FHHHDT ISSN: 0253-3820. Journal written in Chinese. CAN 126:111479 AN 1997:85375 CAPLUS (Copyright 2001 ACS)
Abstract
Cyclic voltammetry and chronocoulometry were used to study the charge transport in polypyrrole film doped with electroactive ions. Charge transport in the film could be treated as the diffusion of electron within the film and the rate of charge transport could be estd. by apparent electron diffusion coeff. Dapp. The value of Dapp was detd. by the structure of film, electron hopping between the electroactive centers and the movement of counterions into and out of film.
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Probe beam deflection combined with cyclic voltammetry studies of C60-modified electrode film. Liu, Changwei; Dong, Shaojun; Cheng, Guangjin; Sun, Da Yong. Changchun Inst. Appl. Chem., Chinese Acad. Sci., Jilin, Peop. Rep. China. J. Electrochem. Soc. (1996), 143(12), 3874-3880. CODEN: JESOAN ISSN: 0013-4651. Journal written in English. CAN 126:217694 AN 1997:15378 CAPLUS (Copyright 2001 ACS)
Abstract
C60 films, prepd. by soln. casting, were studied by in situ probe beam deflection (PBD) combined with cyclic voltammetry (CV). PBD is a powerful technique for study of phenomena at the electrode/electrolyte interface in acetonitrile with quaternary ammonium and alkali metal salts as supporting electrolytes. In tetra-n-butylammonium (TBA+) salt soln., a stable CV can be obtained during the 1st two redn./reoxidn. waves. On redn., injection of cations to maintain charge balance and dissoln. of small amt. of C60-(TBA+) and/or C602-(TBA+)2 are detected. During the reoxidn. process, ejection of cations and injection of anions occur simultaneously, esp. for the 2nd reoxidn. wave. In the case where TBABr is the supporting electrolyte, the accompanied behavior is more complicated than in TBABF4, TBAClO4, and TBAPF6 solns. A small pair of prewaves in CV are proposed due to oxidn./redn. of C60 domains but not dissoln./redeposition of C60 film. Extending the potential scan range to the 3rd redn. wave, no apparent corresponding reoxidn. wave is related to the 3rd redn. wave, the electroactivity of the film disappears rapidly and dissoln. of C60 film is obsd. In tetraethylammonium (TEA+) and NaClO4 solns., the electrochem. of the C60 films is unstable, and potential scans lead to dissoln. of flaking of the film.
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Amperometric biosensor for tyrosinase inhibitors in a pure organic phase. Deng, Qing; Dong, Shaojun. Laboratory of Electroanalytical Chemistry, Chinese Academy of Sciences, Changchun, Peop. Rep. China. Analyst (Cambridge, U. K.) (1996), 121(12), 1979-1982. CODEN: ANALAO ISSN: 0003-2654. Journal written in English. CAN 126:139265 AN 1997:5238 CAPLUS (Copyright 2001 ACS)
Abstract
The detn. of benzoic acid, thiourea and 2-mercaptoethanol in three pure org. solvents, viz., chloroform, chlorobenzene and 1,2-dichlorobenzene, by using an amperometric cryohydrogel tyrosinase biosensor is described. Measurements were carried out with phenol as the enzyme substrate. Kinetic parameters (Ki and I50) were detd. in the three solvents for various inhibitors. The sensor showed the most sensitive measurements to these inhibitors in pure chloroform. The solvent-induced deviation of the biosensor to thiourea was evaluated by means of Hill coeffs. The smallest deviation was obsd. in 1,2-dichlorobenzene,owing to the high hydrophobicity of this solvent. The nature of the inhibition process and its reversibility were also examd.
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Electrocatalytic reduction of nitrite using Dawson-type tungstodiphosphate anions in aqueous solutions, adsorbed on a glassy carbon electrode and doped in polypyrrole film. Xi, Xiaodan; Dong, Shaojun. Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, Peop. Rep. China. J. Mol. Catal. A: Chem. (1996), 114(1-3), 257-265. CODEN: JMCCF2 ISSN: 1381-1169. Journal written in English. CAN 126:95978 AN 1996:737403 CAPLUS (Copyright 2001 ACS)
Abstract
The electrochem. behavior of Dawson-type P2W18O626- adsorbed on a glassy carbon electrode and doped in a polypyrrole film electrode was described. These modified electrodes all display catalytic activity for nitrite redn., either in acid solns. or in pH>4.0 solns.
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Supported phospholipid membranes: comparison among different deposition methods for a phospholipid monolayer. Ding, Lin; Li, Jinghong; Dong, Shaojun; Wang, Erkang. Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, Peop. Rep. China. J. Electroanal. Chem. (1996), 416(1-2), 105-112. CODEN: JECHES ISSN: 0368-1874. Journal written in English. CAN 126:81185 AN 1996:663268 CAPLUS (Copyright 2001 ACS)
Abstract
Supported lipid membranes consisting of self-assembled alkanethiol and lipid monolayers on gold substrates could be produced by three different deposition methods: the Langmuir-Blodgett (L-B) technique, the painted method, and the paint-freeze method. By using cyclic voltammetry, chronoamperometry/chronocoulometry and a.c. impedance measurements, lipid membranes prepd. by these three deposition methods had obvious differences in specific capacitance, resistance and thickness. The specific capacitance of lipid membranes prepd. by depositing an L-B monolayer on the alkanethiol alkylated surfaces was 0.53 mF cm-2, 0.44 mF cm-2 by the painted method and 0.68 mF cm-2 by the paint-freeze method. The specific cond. of lipid membranes prepd. by the L-B method was over three times lower than that of the painted lipid membranes, while that of the paint-freeze method was the lowest. The difference among the three types of lipid membranes was ascribed to the influence of the org. solvent in lipid films and the changes in d. of the films. The lipid membranes prepd. by the usual painted method contained a trace amt. of the org. solvent. The org. solvent existing in the hydrocarbon core of the membrane reduced the d. of the membrane and increased the thickness of the membrane. The membrane prepd. by depositing an L-B monolayer contg. no solvent had higher d. and the lowest fluidity, and the thickness of the membrane was smaller. The lipid membrane prepd. by the paint-freeze method changed its structure sharply at the lower temp. The org. solvent was frozen out of the membrane while the d. of the membrane increased greatly. All these caused the membrane to exist in a tilted state and the thickness of this membrane was the smallest. The lipid membrane produced by the paint-freeze method was a membrane not contg. org. solvent. This method was easier in manipulation and had better reproducibility than that of the usual painting method and the method of forming free-standing lipid film. The solvent-free membrane had a long lifetime and a higher mech. stability. This model membrane would be useful in many areas of scientific research.
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Direct electron transfer to cytochrome c oxidase in self-assembled monolayers on gold electrodes. Li, Jinghong; Cheng, Guangjin; Dong, Shaojun. Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, Peop. Rep. China. J. Electroanal. Chem. (1996), 416(1-2), 97-104. CODEN: JECHES ISSN: 0368-1874. Journal written in English. CAN 126:86503 AN 1996:663267 CAPLUS (Copyright 2001 ACS)
Abstract
A monolayer of cytochrome c oxidase (I) was obtained, which maintained catalytic activity and was attached covalently to self-assembled monolayers of 3-mercaptopropionic acid (II) on a Au electrode. The results of cyclic voltammetry showed that direct electron transfer between I and the electrode surface is a fast and diffusionless process. II played a dual role as both electrode modifier and as a bridging mol. which kept I at an appropriate orientation without denaturation and enabled direct electron transfer between the protein and the modified electrode. Immobilized I exhibited biphasic phenomena between the concn. of the electrolyte and the normal potentials; meanwhile, its electrochem. behavior was also influenced by the buffer components. The quasi-reversible electron transfer process of I with a formal potential of 385 mV vs. SHE in 5 mM phosphate buffer soln. (pH 6.4) corresponded to the redox reaction of cytochrome a3 in I; the heterogeneous electron transfer rate const. obtained was 1.56 s-1. By cyclic voltammetry measurements, it was obsd. that the oxidn. and redn. of cytochrome c in soln. were catalyzed by immobilized I. Thus, this I|II|Au system provides a good mimetic model to study the physiol. functions of membrane-assocd. enzymes and hopefully to build a 3rd-generation biosensor without using a mediator.
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Transmission spectroelectrochemistry. Niu, Jianjun; Dong, Shaojun. Changchun Institute Applied Chemistry, Chinese Academy Sciences, Changchun, Peop. Rep. China. Rev. Anal. Chem. (1996), 15(1-2), 1-171. CODEN: RACYAX ISSN: 0048-752X. Journal; General Review written in English. CAN 125:310864 AN 1996:650528 CAPLUS (Copyright 2001 ACS)
Abstract
A review with 380 refs. is given on spectroelectrochem. (SEC) which is a method allowing simultaneous acquisition of electrochem. and optical information in an electrochem. cell. Transmission SEC includes optically transparent SEC (OT-SEC) and parallel SEC (PL-SEC). OT-SEC involves passing the optical beam directly through transparent electrode and the adjacent soln. PL-SEC lets the optical beam pass parallel through the electrode surface and adjacent soln. The spectroelectrochem. cell, thin-layer spectroelectrochem. , semi-diffusional spectroelectrochem., and anal. spectroelectrochem. are discussed. Electrode reaction kinetics and mechanisms using transmission spectroelectrochem. are discussed.
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A study of the dependence of ferrocene diffusion on chain length in polymer electrolytes. Zhou, Huafang; Dong, Shaojun. Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, Peop. Rep. China. J. Electroanal. Chem. (1996), 414(2), 121-125. CODEN: JECHES ISSN: 0368-1874. Journal written in English. CAN 125:287218 AN 1996:609218 CAPLUS (Copyright 2001 ACS)
Abstract
The diffusion rates of ferrocene were estd. in 5 kinds of poly(ethylene glycol) soln., contg. the electrolyte LiClO4, by using nonsteady-state chronoamperometry. The Dapp of ferrocene increases with increasing temp., and the dependency of Dapp on temp. obeys the Williams-Landel-Ferry equation. The Dapp of ferrocene decreases with increasing polymer chain length. Both the chain length and temp. dependence conform to a simple free vol. model. A relation between current and polymer chain length is suggested at room and high temps.
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Application of self-assembled monolayers in electroanalytical chemistry. Li, Jinghong; Cheng, Guangjin; Dong, Shaojun. Lab. of Electroanalytical Chem., Chinese Academy of Sci., Changchun, Peop. Rep. China. Fenxi Huaxue (1996), 24(9), 1093-1099. CODEN: FHHHDT ISSN: 0253-3820. Journal; General Review written in Chinese. CAN 125:346219 AN 1996:597471 CAPLUS (Copyright 2001 ACS)
Abstract
A review with 33 refs. A crit. review on the self-assemble monolayers (SAMs) for electroanal. applications (esp. take SAMs of thiols on gold electrode as an example) is presented. The emphasis is on the application of SAMs in many fields of electroanal. chem. and electrochem., such as microelectrode, bioelectrochem. and biosensor, liq. chromatog.-electrochem. and FIA, electrocatalysis and spectroelectrochem. etc. The opportunities for the research in the electrochem. and electroanal. applications of SAMs are recommended.
Bibliographic Information
The ion selectivity of monensin incorporated phospholipid/alkanethiol bilayers. Li, Jinghong; Ding, Lin; Wang, Erkang; Dong, Shaojun. Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, Peop. Rep. China. J. Electroanal. Chem. (1996), 414(1), 17-21. CODEN: JECHES ISSN: 0368-1874. Journal written in English. CAN 125:322104 AN 1996:579465 CAPLUS (Copyright 2001 ACS)
Abstract
Monensin was incorporated into phospholipid/alkanethiol bilayers on the gold electrode surface by a new, paint-freeze method to deposit a lipid monolayer on the self-assembled monolayers (SAMs) of alkanethiol. The advantages of this assembly system with a suitable function for investigating the ion selective transfer across the mimetic biomembrane are based on the characteristics of SAMs of alkanethiols and monensin. On the one hand, the SAMs of alkanethiols bring out their efficiency of packing and coverage of the metal substrate and relatively long-term stability; monensin improves the ion selectivity noticeably. The selectivity coeffs. K Na+,K+, KNa+,Rb+ and KNa+,Ag+ are 6´10-2, 7.2´10-3 and 30 resp. However, the selectivity coeff. KNa+,Li+ could not be obtained by a potentiometric method due to the specific interaction between Li+ and phospholipid and the lower degree of complexion between Li+ and monensin. The potential response of this bilayer system to monovalent ions is fairly good. For example, the slope of the response to Na + is close to 60 mV per decade and its linearity range is from 10 -1 to 10-5 M with a detection limit of 2´10-6 M. The bilayer is stable for at least two months without changing its properties. This monensin incorporated lipid/alkanethiol bilayer is a good mimetic biomembrane system, which provides great promise for investigating the ion transfer mechanism across the biomembrane and developing a practical biosensor.
Bibliographic Information
The fabrication and performance of a Ag/AgCl reference electrode in human serum. Yu, Pengguang; Dong, Shaojun. Lab. Electroanalytical Chem., Chinese Acad. Scis., Changchun, Peop. Rep. China. Anal. Chim. Acta (1996), 330(2-3), 167-174. CODEN: ACACAM ISSN: 0003-2670. Journal written in English. CAN 125:296351 AN 1996:579189 CAPLUS (Copyright 2001 ACS)
Abstract
A simple set of elec. circuits was used to assemble a pulse generator. With pulse potentials and under galvanostatic control, a clean silver wire was anodized electrochem. for 0.2-0.5 min in 1.0M HCl with a pulse c.d. of 20 mA/cm, and the pulse wave parameters of ta/tc = 1 and a cycle of 4 s forming a Ag/AgCl ref. electrode. Even though the AgCl layer was consumed during the working period when the Ag/AgCl electrode was used as a cathode, the AgCl layer could be in situ recovered electrochem. in serum used when a reversed potential was applied to the electrode system immediately after the measuring program was finished. The current response curve of the anode indicated that a AgCl layer of high d. was basically accomplished during the 1st 6 pulse cycles in human serum. To keep a stable and uniform AgCl layer on the ref. electrode after each measuring cycle, the ratio of the recovery time (tr) to the working time (tw) was measured and the smallest value was obtained at 0.03. The open-circuit potential of the Ag/AgCl electrode with respect to a SCE in 0.1M KCl was monitored over a period of 14 days and the mean value was 40.09 mV vs. SCE with a std. deviation of 2.55 mV. The potential of the Ag/AgCl ref. electrode did remain const. when the measurements were repeated >600 times in undild. human serum with a std. deviation of 1.89 mV. This study indicated that the Ag/AgCl ref. electrode could be rapidly fabricated with a pulse potential and could be used as a ref. electrode with long-term stable properties in human serum samples.
Bibliographic Information
Electrochemical identification of intermediate forms of urea denaturation of horse heart cytochrome c. Zhu, Yimin; Dong, Shaojun. Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, Peop. Rep. China. Bioelectrochem. Bioenerg. (1996), 41(1), 107-113. CODEN: BEBEBP ISSN: 0302-4598. Journal written in English. CAN 125:269639 AN 1996:566454 CAPLUS (Copyright 2001 ACS)
Abstract
The electrochem. identification of the urea denaturation of horse heart cytochrome c in bulk soln. at the 4,4'-dithiodipyridine -modified gold electrode is reported. The results are similar to the three-step transitions of equil. studies (Myer et al., Biochem., 19 (1980) 199) of urea denaturation of cytochrome c in bulk soln. This method permits a clear resoln. of which of the three steps of urea denaturation is electrochem. related. In addn., by analyzing the effects of urea on the structural forms of cytochrome c and on the soln. properties, as well as the cyclic voltammetric responses of the protein, the individual forms of the urea denaturation of cytochrome c can be understood. The results reflect the superposition of protein denaturation on the electrode surface and in soln.
Bibliographic Information
Interaction of brilliant cresyl blue and methylene green with DNA studied by spectrophotometric and voltammetric methods. Liu, Junli; Li, Jinghong; Dong, Shaojun. Inst. Applied Chem., Chinese Academy Sciences, Changchun, Peop. Rep. China. Electroanalysis (1996), 8(8-9), 803-807. CODEN: ELANEU ISSN: 1040-0397. Journal written in English. CAN 125:215023 AN 1996:554615 CAPLUS (Copyright 2001 ACS)
Abstract
The binding behavior of two cationic dyes, brilliant cresyl blue (BCB) and methylene green (MG) to calf thymus DNA was studied by spectrophotometry and voltammetry. A red shift of the absorption spectra and hypochromism accompanied the binding of BCB and MG to calf thymus DNA. In 5 ´ 10-2 mol/dm3 NaCl, 5 ´ 10-3 mol/dm3 tris-HCl pH 6.87 buffer soln., the apparent binding consts. are: KBCB+ 3.0 ´ 104 M-1 (N = 4.13) and KMG+ = 8.8 ´ 104 M-1 (n = 4.44). The formal potentials shifted neg. upon addn. of DNA, indicating that the oxidized forms of the dyes have stronger affinity to DNA than the reduced ones. KBCB+/KBCBH and KMG+/KMGH are evaluated to be 10.39 and 7.04, resp. The 2 cationic dyes interacted with DNA predominantly via electrostatic interaction.
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Cryoimmobilized enzyme for biosensor construction. Wang, Ying; Guo, Yizhu; Zhu, Guoyi; Dong, Shaojun. Changchun Inst. Applied Chemistry, Chinese Academy Sciences, Changchun, Peop. Rep. China. Sens. Actuators, B (1996), B31(3), 193-196. CODEN: SABCEB ISSN: 0925-4005. Journal written in English. CAN 125:215676 AN 1996:547840 CAPLUS (Copyright 2001 ACS)
Abstract
A new immobilization material and an immobilization method for a glucose sensor with HEFc (hydroxyethylferrocene) as mediator is described. In the course of three months, the enzyme electrode shows almost no deterioration in its response characteristics. The response time is less than 30 s. The electrode has a wide linear range up to 10 mmol l-1 with good repeatability. The kinetic parameters have also been calcd.
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Study of SiW12 heteropolyacid modified microelectrode. Tian, Min; Xi, Xiaodan; Dong, Shaojun. Lab. Electroanalytical Chem., Changchun Inst. Appl. Chem., Chanchun, Peop. Rep. China. Fenxi Huaxue (1996), 24(8), 902-905. CODEN: FHHHDT ISSN: 0253-3820. Journal written in Chinese. CAN 125:264427 AN 1996:547143 CAPLUS (Copyright 2001 ACS)
Abstract
The electrochem. behavior of SiW12 heteropolyacid on conventional electrode and microelectrode was studied. The effect of soln. pH on the electrochem. behavior of SiW12 was discussed. The modified microelectrode of SiW12 heteropolyacid was made, and its electrocatalytic redn. of nitrite was assessed. It showed that the modified microelectrode greatly reduced the overpotential of nitrite redn.
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Detection of Hydrazine, Methylhydrazine, and Isoniazid by Capillary Electrophoresis with a Palladium-Modified Microdisk Array Electrode. Liu, Jun; Zhou, Weihong; You, Tianyan; Li, Fenglei; Wang, Erkang; Dong, Shaojun. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Peop. Rep. China. Anal. Chem. (1996), 68(19), 3350-3353. CODEN: ANCHAM ISSN: 0003-2700. Journal written in English. CAN 125:211423 AN 1996:529613 CAPLUS (Copyright 2001 ACS)
Abstract
A palladium particle-modified carbon fiber microdisk array electrode was designed and employed in capillary electrophoresis for the simultaneous detection of hydrazine, methylhydrazine, and isoniazid. The Pd-modified microdisk electrode had high catalytic ability for hydrazines and exhibited good reproducibility and stability. The response for hydrazine was linear over 3 orders of magnitude with a correlation coeff. of 0.993. The detection limits for hydrazine, methylhydrazine, and isoniazid were 1.2, 2.1, and 6.2 pg, resp.
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Acetylcholinesterase amperometric detection system based on a cobalt(II) tetraphenylporphyrin-modified electrode. Deng, Qing; Dong, Shaojun. Lab. Electroanalytical Chem., Changchun Inst. Applied Chem., Changchun, Peop. Rep. China. Analyst (Cambridge, U. K.) (1996), 121(8), 1123-1126. CODEN: ANALAO ISSN: 0003-2654. Journal written in English. CAN 125:161732 AN 1996:512991 CAPLUS (Copyright 2001 ACS)
Abstract
An acetylcholinesterase (AChE) activity detection system was fabricated based on the electrocatalysis of cobalt(II) tetraphenylporphyrin of the electrooxidn. of thiocholine chloride, which is the product of the hydrolysis of acetylthiocholine chloride by AChE. A simple modified method was used to form the base electrode. AChE was cross-linked on the base electrode by glutaraldehyde. The optimum working conditions are discussed and the characteristics of the detection system are evaluated.
Bibliographic Information
Study on ferrocene derivatives diffusion dynamics in polymer electrolyte by solid-state voltammetry. Zhou, Huafang; Dong, Shaojun. Lab. Electroanalytical Chem., Chinese Acad. Sci., Changchun, Peop. Rep. China. Electrochim. Acta (1996), 41(15), 2395-2398. CODEN: ELCAAV ISSN: 0013-4686. Journal written in English. CAN 125:206845 AN 1996:487288 CAPLUS (Copyright 2001 ACS)
Abstract
The diffusion rates of seven ferrocene derivs. were estd. in polyelectrolyte PEG×LiClO4 by using nonsteady-state chronoamperometry. The Dapp of ferrocene derivs. increases with temp., and the dependency of Dapp on temp. obeys the Arrhenius equation. The Dapp of ferrocene derivs. decreases with increasing size of electroactive species. The DDapp values of DT>Tm and DT Tm in the polyelectrolyte. However, the diffusion behavior of ferrocene derivs. is qual. analyzed by using cyclic voltammetry.
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Influence of nicotinic acid or its isomers on direct electrochemistry of cytochrome c. Qu, Xiaogang; Lu, Tianhong; Dong, Shaojun. Changchun Institute Applied Chemistry, Chinese Academy Sciences, Changchun, Peop. Rep. China. Chin. Sci. Bull. (1996), 41(4), 348-349. CODEN: CSBUEF ISSN: 1001-6538. Journal; General Review written in English. CAN 125:107920 AN 1996:471232 CAPLUS (Copyright 2001 ACS)
Abstract
A review, with 3 refs. The position of the functional group in the promoter mol. affects the interaction between cytochrome c and the promoter, and further the promoting effect of the compd. The use of isomer-modified functional electrodes is important for biomol. mimic recognition.