İNŞaat mühendiSLİĞİ anabiLİm dali

Effect of Vitamin U on Liver Injury Induced by Amiodarone in Rats
Amiodarone, 2-butyl-3-(3’,5’-diiodo-4’--diethyl amino etoxy benzoyl)-benzofuran, is an antiarrhtymic drug that is used for prevention of the diseases like heart rhythm disorders and tachycardia nowadays. This drug shows toxic effect by accumulation specially in liver and many tissues and organs. Vitamin U, S-methylmethionine sulfonium chloride, has mainly lipid lowering, and besides antiulcer, antidepressant and antioxidant effects. The aim of this study was the investigaton of the protective effect of Vitamin U on liver injury induced by amiodarone. In this study, 3,5-4 months old Sprague-Dawley male rats were randomly divided into four groups. Group I; control animals receiving corn oil for 7 days. Group II; animals receiving Vit U (50 mg/kg) for 7 days. Group III; animals receiving amiodarone (100 mg/kg) for 7 days. Group IV; animals receiving Vit U (50 mg/kg) for 7 days 1 h prior to the administration of amiodarone. Amiodarone and vitamin U were administered by gavage to rats. On the 8^th day, all the animals which were fastened night over were sacrificed, blood and liver samples were taken under anesthesia. In serum samples, aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase enzyme activities and cholesterol, total lipid and total bilirubin levels were determined. In liver samples, glutathione and lipid peroxidation levels and lactate dehydrogenase, gamma glutamyl transferase, myeloperoxidase, paraoxonase, adenosine desaminase, xanthine oxidase, arginase, sodium potassium ATPase, prolidase and DT-diaphorase and thromboplastic activities, protein carbonyl content and tissue factor, fucose, hexose and hexosamine levels were measured. It was determined that serum aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase activities and cholesterol, total lipid and bilirubin levels of amiodarone group made an increase when compared with control group. In liver tissue samples of amiodarone group, it was found that lipid peroxidation levels and lactate dehydrogenase, gamma glutamyl transferase, myeloperoxidase, adenosine desaminase, xanthine oxidase, arginase, prolidase, DT-diaforase and thromboplastic activities, protein carbonyl content, fucose, hexose and hexosamine levels were increased compared to control group, glutathione levels and paraoxonase and sodium-potassium ATPase activities were decreased as compared to control group. Treatment with vitamin U reversed these effects. These results demonstrated that administration of vitamin U is a potentially beneficial agent to reduce the liver damage in amiodarone hepatotoxicity.

DUMAN Songül

Danışman : Prof. Dr. Bahri ÜLKÜSEVEN

Anabilim Dalı : Kimya

Programı : Anorganik Kimya

Mezuniyet Yılı : 2014

Tez Savunma Jürisi : Prof. Dr. Bahri ÜLKÜSEVEN

Prof. Dr. İrfan KIZILCIKLI

Prof. Dr. Esma TÜTEM

Prof. Dr. Ulvi AVCIATA

Prof. Dr. Ahmet Gül