Figure 7: ROS-induced mitochondrial alterations in C2C12 muscle cells. A- ROS production, measured by NBT reduction, measured in differentiated C2C12 myotubes incubated with glucose (25mM) or palmitate (200µM), in presence or not of NAC (10mM), for 96 hours (n=4). Data are expressed relative to respective control (dotted line). B- Effect of H2O2 (0.1mM) , glucose (25mM) and palmitate (200µM) on mtDNA levels in differentiated C2C12 myotubes. Myotubes were treated for 96 hours in presence or not of 10mM NAC (n=4). Data are expressed relative to control condition (dotted line). C: Citrate synthase (CS) activity measured in total lysates of myotubes treated for 96 hours with H2O2 (0.1mM), glucose (25mM) or palmitate (200µM), in presence or not of NAC (10mM) (n=4). D: mRNA levels of POLG2, SSBP1 and PGC-1 genes, determined by real-time RT-PCR, in H2O2, glucose or palmitate-treated myotubes, in presence or not of NAC, for 96 hours (n=4). All results are expressed as fold change relative to the values of untreated cells set to one unit (dotted line). *p<0.05, ** p<0.01 vs. respective control. # p<0.05 with and without NAC for each treatment.